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Gene information
General information
TC010319 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451153.1: 25978..27019
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010319
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04807

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010319 RA
      MSLTNYETA FTAILFVPC VYMFKKLYS 
      TYKSLKQQY EDEELFYQR HNCVVMYSK 
      THITGWPPE YKMSISTKN LDKFLDPYL 
      YFINTSKHS IDLAVMTFS LKPLMEALQ 
      SALTRGVKV RLIVNYLSV KNQAKQYNE 
      LMKSGIKVA FYIEKTTSL SNIMHCKYS 
      VKDYDGSKG FLFMGSMNL TGMSTILNN 
      YEDVTFTSN IYLVETFHK SFQDSWNMI 
      MEDNENFYN KTVLADSGF SV
    CDS sequence
    • >TC010319 RA
      atgtcttta acaaattac gaaacagct tttaccgca 
      attttgttc gtgccctgt gtttacatg ttcaaaaag 
      ctttattca acctacaag tccttaaaa cagcagtat 
      gaggacgaa gaactattc taccaaaga cacaactgt 
      gttgttatg tactccaag actcacatc actggctgg 
      cctcccgaa tataaaatg agcatttcg accaaaaat 
      ttggacaaa tttttggat ccttatttg tattttata 
      aatacatca aaacactcc attgacctg gctgtgatg 
      accttttca ttaaaacct ttaatggag gctttacaa 
      agcgcttta acacgtggg gttaaagta agactgatt 
      gtaaattat ttatcggtc aaaaaccaa gccaaacaa 
      tacaatgag ttgatgaaa tcagggatt aaggtggcc 
      ttttatatt gagaaaact accagtctg agtaatata 
      atgcattgt aaatatagt gtcaaggat tatgatgga 
      tccaagggt tttctcttt atgggttca atgaattta 
      actggaatg tcaacaata ttaaataat tatgaagat 
      gttactttc acgtcgaat atttatctt gttgaaaca 
      ttccataaa agttttcaa gattcgtgg aatatgatt 
      atggaagat aatgaaaat ttttacaac aagactgtt 
      ctagctgat agtggcttt tcggtttga
    mRNA sequence
    • >TC010319 RA
      caaaaactg tgataaaac aacgtgtaa acatgtctt 
      taacaaatt acgaaacag cttttaccg caattttgt 
      tcgtgccct gtgtttaca tgttcaaaa agctttatt 
      caacctaca agtccttaa aacagcagt atgaggacg 
      aagaactat tctaccaaa gacacaact gtgttgtta 
      tgtactcca agactcaca tcactggct ggcctcccg 
      aatataaaa tgagcattt cgaccaaaa atttggaca 
      aatttttgg atccttatt tgtatttta taaatacat 
      caaaacact ccattgacc tggctgtga tgacctttt 
      cattaaaac ctttaatgg aggctttac aaagcgctt 
      taacacgtg gggttaaag taagactga ttgtaaatt 
      atttatcgg tcaaaaacc aagccaaac aatacaatg 
      agttgatga aatcaggga ttaaggtgg ccttttata 
      ttgagaaaa ctaccagtc tgagtaata taatgcatt 
      gtaaatata gtgtcaagg attatgatg gatccaagg 
      gttttctct ttatgggtt caatgaatt taactggaa 
      tgtcaacaa tattaaata attatgaag atgttactt 
      tcacgtcga atatttatc ttgttgaaa cattccata 
      aaagttttc aagattcgt ggaatatga ttatggaag 
      ataatgaaa atttttaca acaagactg ttctagctg 
      atagtggct tttcggttt gaagtttat ttctaatac 
      aatatttat gatttatat tttacttct attatttga 
      tatttttcg ttaatattt ttatttctg ttccatgcg 
      ttattcgca tgtcactgg aaaaccttc cttgaaaat 
      gaaaaaatc gcttttgtt gggtaattt agtggaata 
      tttggaaat ttttagtca aaagtttct cgacgactt 
      aaaaaaatt gaagatttc cgttttttt tttttaata 
      aaaaagcat taattca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY