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Gene information
General information
TC010304 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450940.1: 4..4486
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC010304
      iBeetle screen iB_04804

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC010304 RA
        MSMKNTLEA LQTIARVEK SQQSAEVFK SYKRSYFQE 
        IEENHRNYN AYILNNADN KQKAIWKII NTKTKTSNT 
        KECKLNADD LNLYFATIG EKTSDSIKK IDKDPCDFL 
        KACKSNAKS FWLAPVSEE EVTRLIRSL KCKNSKDVS 
        GRSTITAAL DLYKKITRA LDNREMVEV AMCDLSKAF 
        DTLPHKLIL QKLQHYGIR GITQEIFKS YLENRTQIV 
        KWNEKWSKP LPTRCGVPQ GSILGPLLF IIYVNDLPI 
        NMRVDGQCL YADDTSFCI KEETEDTLK PKMRKILED 
        ANEWFTANK LKLNGDKTQ ELFISSKPT NVTSVKFLG 
        FYLNSKASW KDHIQETCK NLASALFSI RRIKNISTP 
        QAARVTYFA NFHSKATYG ILLWGTSSD ADKIFVLQK 
        KAIRILCQM NYRESCRPA FKQQQILTI PSAYILSCL 
        RYVHNHLHE FIKNGSYHE HNTRHADAL STPYHRLKV 
        SQHSIDHWG PKLYNKLQD PMKRLAPAL FEKKVKDAL 
        LEMSYYSIQ EFLDNPPDL K
      CDS sequence
      • >TC010304 RA
        atgagtatg aaaaataca ctagaagct ctacaaaca 
        atagcaaga gttgagaaa agccaacaa tcagctgag 
        gtcttcaag tcatataaa cgcagctac ttccaagaa 
        attgaagaa aaccacaga aactacaac gcttatatc 
        ctaaacaat gctgataat aagcaaaaa gccatttgg 
        aaaatcatc aatacaaaa acgaagaca agcaatacc 
        aaagaatgc aaattgaat gccgacgac ctcaatctc 
        tactttgcc acgatagga gagaaaacg tcagacagt 
        ataaaaaaa atcgacaaa gatccttgt gatttcctc 
        aaggcgtgt aaaagcaat gcaaaatcg ttttggtta 
        gcgcctgtt agtgaagaa gaggtcaca agactgata 
        agatcctta aaatgcaaa aactccaaa gatgtaagt 
        ggtagatca accattact gcagcattg gatctatac 
        aagaaaata acaagagcc ctagataac cgagaaatg 
        gtagaagta gcgatgtgc gatctaagc aaagcgttt 
        gacaccctg ccacacaag ttgattcta caaaaatta 
        caacactat gggatacga gggatcacg caagaaatt 
        tttaagtcc taccttgaa aatagaacc cagatagta 
        aaatggaat gaaaaatgg tcaaagcct ctgccaaca 
        aggtgtgga gtgccccag ggatcaatc ctcggtcca 
        ctgctattt ataatctat gtaaatgac ttgcctatc 
        aatatgaga gtggatgga cagtgtcta tatgcggac 
        gacacttcc ttctgcatc aaagaagaa acagaggat 
        acgctaaaa ccaaaaatg agaaaaatc cttgaagat 
        gccaatgaa tggttcaca gcaaataaa ctgaaactc 
        aatggagac aagactcag gaactattt atatcttca 
        aaacccacc aacgtcaca tcggtaaaa ttcttggga 
        ttttaccta aatagcaaa gcatcttgg aaggaccac 
        attcaggaa acctgcaaa aacctagca tcagcttta 
        ttcagtatc agaagaatc aagaatatc tcaactccc 
        caagctgca agggtgaca tactttgct aacttccac 
        agcaaagcc acctatggt atacttcta tggggtact 
        tcatcagat gctgacaag atcttcgtc ctgcaaaag 
        aaagcaatc agaatcctg tgccaaatg aactatcgg 
        gaaagctgc cgcccagcc ttcaaacaa caacaaatt 
        cttaccatc ccttccgcg tacatattg agctgtctg 
        aggtacgtc cacaaccac ctccacgag ttcatcaag 
        aatggctca tatcacgag cataacaca agacacgct 
        gacgcgctc tcaacaccg taccatcgg cttaaagtg 
        tcacagcac agcattgac cattggggc ccgaagctt 
        tataacaag cttcaggac ccgatgaag agactagct 
        ccggctctg tttgagaag aaggtaaag gacgcacta 
        ttagaaatg agttattat tctatccaa gagttcctg 
        gataaccct ccagattta aaataa
      mRNA sequence
      • >TC010304 RA
        ggtcattct tataagctg aaaaaagaa ccatttaag 
        actaccaat cagaccctc gcaagagga agcgatgag 
        acaccttat agacattgt aagtaacct taagggaaa 
        aattgacga ctgtctgaa acaaataca aaatcaaaa 
        cggaacaat acaaaggtt agatcaact gttagcgga 
        gtcacaaac caaatcgtc caaaccaat aacaagatc 
        caacaaagg acacacaga tgtcatcac aaaccaaga 
        aagagaaaa cctgacgaa cgtaaaagc tcagaaaaa 
        aagtaacaa gccatctga cagggtgat agaaaacaa 
        caagcaaaa aaaaacaag ttgacaagg agggtaaca 
        cagtccagg acaaaaaac ccgaaacaa gccaatcat 
        taaaccaac gaaacgacg aaaaagaaa aagacatca 
        ggacaaagg ttgcaaaaa aatcgaaac aggacgtaa 
        tcacaggga cagctagag tagatgagg ctgacggct 
        tcagaggcg cccgtaaga tgggatggc tgtttgttg 
        gccgtgcaa caagtacta cgacagaag ataaagtat 
        caaattatc tcaaacaac tacatcctg atgaacatt 
        tcaccgtgg aagaactac ccaagcaca tcaacaata 
        agagtcaaa ataaatcct ttaaagttg gactacgat 
        ttgaattag tagaagaaa tgatgaaac ctgagtcgt 
        ggcccgaaa acctaacca tacagaggt tcaagtttt 
        tttcgaaga ccccaacaa atcggtagg ttttgaaaa 
        cctaaccac aataataaa ctataacat acaatcact 
        atcgaaaaa aatagacgc actgactat acagttaac 
        agaagacga agtagacat tgtaattat cactgagca 
        ctggtgcaa ggacaacac cctggaaag cttaaccat 
        cccaggata taatattat aagccactt tagcagatc 
        acttaacca gcatggcgg atccttaat agcaacaaa 
        ggagggtat ttccgcaaa aaatattga aggcattag 
        acccctatg tgaagaatt cactgcaga aatctcagc 
        tgctgagat tccttcaaa aaacttaat aataatggc 
        ggtatatcg atctccaaa tcagaactt caagcactt 
        catagataa aattaatga agcactaga aaacctgac 
        acaaaggaa ccactctag acgatacat tgtcgctgg 
        ggactttaa caccaatat tctcaagga tggcgtcga 
        acaatcttt attaatcga aactttcac aacatatgg 
        tatgcaacc actaatgag agaggcatc tagaacaac 
        tgaaacttc ttctagctg catagataa tatattttc 
        aaatattaa catagaaga aatcttctc aaaagagac 
        aaaaagttg gtgcatgtc tgatcacta ctgtcaagt 
        cctgaagat taagaaccc agtgcaaga acctagaaa 
        accagaaaa acatatata cagaagaat aaatgaaca 
        gaatactta ctactttaa ggagtactt aaccggaat 
        tgagtgggc tcgtgtctt caaacaccg taatgcaaa 
        agaggccta cactgtctt ccatacaac tctgacagc 
        tgcctttga caaattctt ccctagaaa agaaaaaat 
        taataaacc aaaattcaa taagagacc tacgtggat 
        tagtaaaaa aatcatgag tatgaaaaa tacactaga 
        agctctaca aacaatagc aagagttga gaaaagcca 
        acaatcagc tgaggtctt caagtcata taaacgcag 
        ctacttcca agaaattga agaaaacca cagaaacta 
        caacgctta tatcctaaa caatgctga taataagca 
        aaaagccat ttggaaaat catcaatac aaaaacgaa 
        gacaagcaa taccaaaga atgcaaatt gaatgccga 
        cgacctcaa tctctactt tgccacgat aggagagaa 
        aacgtcaga cagtataaa aaaaatcga caaagatcc 
        ttgtgattt cctcaaggc gtgtaaaag caatgcaaa 
        atcgttttg gttagcgcc tgttagtga agaagaggt 
        cacaagact gataagatc cttaaaatg caaaaactc 
        caaagatgt aagtggtag atcaaccat tactgcagc 
        attggatct atacaagaa aataacaag agccctaga 
        taaccgaga aatggtaga agtagcgat gtgcgatct 
        aagcaaagc gtttgacac cctgccaca caagttgat 
        tctacaaaa attacaaca ctatgggat acgagggat 
        cacgcaaga aatttttaa gtcctacct tgaaaatag 
        aacccagat agtaaaatg gaatgaaaa atggtcaaa 
        gcctctgcc aacaaggtg tggagtgcc ccagggatc 
        aatcctcgg tccactgct atttataat ctatgtaaa 
        tgacttgcc tatcaatat gagagtgga tggacagtg 
        tctatatgc ggacgacac ttccttctg catcaaaga 
        agaaacaga ggatacgct aaaaccaaa aatgagaaa 
        aatccttga agatgccaa tgaatggtt cacagcaaa 
        taaactgaa actcaatgg agacaagac tcaggaact 
        atttatatc ttcaaaacc caccaacgt cacatcggt 
        aaaattctt gggatttta cctaaatag caaagcatc 
        ttggaagga ccacattca ggaaacctg caaaaacct 
        agcatcagc tttattcag tatcagaag aatcaagaa 
        tatctcaac tccccaagc tgcaagggt gacatactt 
        tgctaactt ccacagcaa agccaccta tggtatact 
        tctatgggg tacttcatc agatgctga caagatctt 
        cgtcctgca aaagaaagc aatcagaat cctgtgcca 
        aatgaacta tcgggaaag ctgccgccc agccttcaa 
        acaacaaca aattcttac catcccttc cgcgtacat 
        attgagctg tctgaggta cgtccacaa ccacctcca 
        cgagttcat caagaatgg ctcatatca cgagcataa 
        cacaagaca cgctgacgc gctctcaac accgtacca 
        tcggcttaa agtgtcaca gcacagcat tgaccattg 
        gggcccgaa gctttataa caagcttca ggacccgat 
        gaagagact agctccggc tctgtttga gaagaaggt 
        aaaggacgc actattaga aatgagtta ttattctat 
        ccaagagtt cctggataa ccctccaga tttaaaata 
        aatgtatgt attgtccca tatacactt acatgtaca 
        taattatat gacgcgcca tgtattatt tattgatgg 
        caataaaat ctataatct ata
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY