Status
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Gene information
General information
TC031763
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010279,TC010278,TC010277,TC010280
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451049.1: 110063..139461
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC031763
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
      {{ blurEvidenceResult }}
      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_04803

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection


      eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 5 (16 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC031763 RA
        MSGKEHRKV VTDQGVGSP DPAGVELLA RGSPRSSSS 
        DRDAVRLGN QGTLQEIAT GTVKKRGKD EGKPTGTTL 
        VHSRLNFMV GNIQRSNSL SHAEKKRKY MDTSAENLN 
        PEDGVYIKK ECPEVFVLK EELNQIVRL VKELEKKTG 
        EMYNPKKEL VNLVSKLSR SVQVLNRTS ILTWLENHR 
        YEPVEKMTI DADVQTDAE SSGDEVQTK TPKATMVDC 
        ATQIENWSQ RRPELTTWT QVTNFKEFQ EISKKEWPS 
        NIYSSTEIK IGNPLATKD SVVKVVFVE PEDTQMTRS 
        IQSLYKDRY PELLEIEED YGTLEQVTK IGNMESRRK 
        VIKILATNP EDLWEKLNR LKENTDNGE WIVMHHLTW 
        MDNKQLQKV VEAVFHETE TKVVVQSGG WYCDLRIDA 
        AVKVLSRAV SVIDCRSGN GFVWIETDK YKIYSCYIS 
        PNCAYEEYH ETLERLRDH LVVNRNRSL VTGDFNAKS 
        YLWGNVSED KRGKELADL IGELEMWVL NQGNQPTFV 
        RGQSSSIID ITFASPKVA QCVKGWHVH EDAPLGDHL 
        PIVFALQPQ RNRANEGEP PSRWRWSET CRERLFASL 
        RNKLKSHMA ISPEKLTSL VIEACKENL TRYESGGGS 
        RRKVYWWND QIRSSRTKC VKARRTLMR ANRRKRKEA 
        IELMTLEAD YAIAKKELR KQIKIAKDN AWKKLIDEL 
        EEDPWGKTY QVVMKKLKR ETPPTLNEK LRNVKALFP 
        QKIPILWEP PMVQTWDPF TREELATAA AKIRVKKAS 
        GPDGIPPEV VRLACEAVP EVVLGTMNR IMKSGVFPP 
        NWKTARLVL IPKKGMDEE GNRKMRPIC LLDVFGKLL 
        EHLIRARLN EDIDDNGGL SDQQFGFRE GRCTLDAVT 
        EVMKLAKFA NNGTWGRKD YCALVTLDI ENAFNSASW 
        LHIIEQLRR RGIKKELLR LIMSYLEGR RIEVTPNHI 
        VDITCGVPQ GSVLGPSLW NILYDGVLK QDMPLGVKL 
        VAFADDLAV AAIGRNEED LMRNTNQAL ARVASWLEE 
        NELKLAAEK TEAVLLVGR RRPRAVQFQ IMGAKIEPK 
        KVIKYLGIY IDQACTFSD HLAKTAAKA EGTISALSR 
        IMPNIGGPG SNRRKLLAG VATSMLLYG CEVWHTALA 
        TKKNVVVLE STHRRILAR VACAYRTVS TEAAQVITG 
        VPPARLLVE ERVKQRCTG MSKSMARSH TLNLWQENW 
        QQTTKARWT RRLIPDIEK WVKRKHGEI DYHLTQALT 
        GHGCFGTYL CRIGKILNA SCWYCGEED TPSHTLFSC 
        SYFDPLRMD VMKKILVWP EETEKENLG PVIPVQCYR 
        RRAAEALKI AENTIKNVI KENLGEKEA PQNTRPKKK 
        KKTEDVCNS VKMDVRQTI YQMIKENTY VTIKGLKQV 
        LEDKRIYQG SWSSLRRLL LSIGFHYKR DCNRRALCE 
        KPQIVSKRL NFLRKYKEY KAMNLYDFV FLDETWIFA 
        KGGNRHSWQ DDSIKSCRS ANVGSVVKG HKTSAEAEV 
        PARTTSRVG NDRGQLDAI LTTAVLAMT VSDREKMSV 
        EEQQRQTIA NLEQQLAEA RAAQAALQS DRTGTRDID 
        ANIEEIKNT NLKLPSFWQ RDPALWFAQ VEAQFHAYR 
        IKADQSRYY TVVAALDCS VLQAVSDIL TNPPETNKY 
        ETIKTKLIS AYSDSQEKQ LRRLLNELE LGDRKPSQL 
        LREMRTLAG VQVNEEVLR TLWLQRLPA QIHLWLSAS 
        ESTGIAVSN SRPSEMMAP D
      CDS sequence
      • >TC031763 RA
        atgagcggc aaagaacac aggaaagtt gttactgat 
        cagggggta ggatcccca gatccggca ggggtggag 
        ttgttagcg cggggctcc cccaggtcg tcatccagc 
        gaccgcgat gcagtgcgg ctagggaac cagggcaca 
        ctgcaggaa atagcgact ggaacggtc aagaaacgt 
        gggaaagat gaaggaaaa ccgactggc acaacactt 
        gtgcatagc cgacttaac tttatggta ggaaacata 
        cagagaagt aactctctt agtcacgca gaaaagaaa 
        aggaagtat atggatact tcagcggag aacctcaat 
        ccagaggat ggtgtatat attaagaaa gaatgccca 
        gaagtattc gtactgaag gaggaactc aaccagatt 
        gtcagactg gtgaaagaa cttgagaaa aaaaccgga 
        gaaatgtat aacccaaaa aaagaactg gttaatcta 
        gttagcaaa ctttcccga agcgttcag gttttgaac 
        agaacttca atcctgaca tggctggaa aaccatagg 
        tatgaaccc gtcgaaaag atgacgata gatgcggat 
        gtgcaaaca gacgcagag agcagcgga gatgaagta 
        cagactaag acaccaaaa gctacgatg gtagactgc 
        gccacacaa atagaaaat tggagtcaa agaaggccc 
        gaacttacg acttggacg caagtaaca aattttaag 
        gagtttcag gaaatatca aaaaaggag tggccatct 
        aacatatat agcagcacg gaaatcaaa atcggaaat 
        cctctagcg acaaaagac agcgtagta aaggtggtt 
        tttgtagaa ccagaagat acacaaatg acgcgaagt 
        atacagtca ctatataaa gatagatat ccagaactt 
        cttgaaatc gaagaagat tatggtacc ctggaacaa 
        gttacaaag ataggaaat atggaaagc cgccgaaaa 
        gttatcaag attcttgcc acaaatcct gaagacttg 
        tgggaaaaa ttgaatagg ttaaaagaa aatacagat 
        aacggggaa tggatcgtt atgcaccat cttacttgg 
        atggataat aagcagctc cagaaagtt gtggaagca 
        gtttttcat gaaaccgaa acaaaggtg gtagtgcaa 
        tcgggtgga tggtattgt gatctccga atcgacgct 
        gctgtcaaa gttttatcg agggcggtc agcgtaatc 
        gactgccgg tcaggaaat ggctttgtt tggatcgaa 
        acggataag tataagatc tacagttgt tacatatcc 
        cccaattgt gcttacgag gaatatcat gagactctc 
        gaaagacta cgggatcac ttagtagtc aaccgtaac 
        cgctctctt gtaaccggt gacttcaat gccaaatcg 
        tatttatgg ggaaacgtg agcgaagat aaaagaggc 
        aaggagtta gcggatcta atcggtgaa ctagagatg 
        tgggtgcta aatcagggc aatcagccg actttcgtc 
        agaggacaa agctcatca ataattgat ataactttt 
        gcttcccca aaggtagct caatgtgtg aagggttgg 
        catgttcac gaggatgcc cctctaggt gatcacttg 
        ccgattgtt ttcgctcta cagccccag agaaaccgc 
        gcaaacgaa ggagaacct ccaagcaga tggcgatgg 
        tccgaaaca tgccgggag agactattt gcctcccta 
        agaaacaaa ttaaaatca catatggcg atatcccca 
        gagaaatta acctctttg gtgatagaa gcctgcaag 
        gagaatctc acgagatac gaaagcggg ggaggtagc 
        agacgcaag gtctactgg tggaacgac caaataaga 
        agcagtagg acaaaatgc gtgaaggcc agaagaact 
        cttatgcgc gctaacaga aggaaacgg aaagaagca 
        atagagttg atgactcta gaagccgat tacgctatc 
        gctaaaaaa gagcttcgg aagcaaatt aaaattgcc 
        aaggacaat gcctggaaa aagcttatt gacgagttg 
        gaagaggat ccatgggga aaaacatac caagttgta 
        atgaaaaag ctaaaaaga gaaacgccc ccaactctg 
        aacgagaag ttgcgaaac gttaaggca ctattcccc 
        caaaaaatt ccaatctta tgggagcca ccgatggtt 
        cagacatgg gatccgttt actagggaa gagttggca 
        acagcggcg gctaaaatt agggttaaa aaagcctcc 
        ggcccggat gggatacca ccagaggtc gtgcgcctt 
        gcatgcgag gcagtacct gaggtggtc ctcgggaca 
        atgaatcgg atcatgaaa tcgggagtt ttccctccg 
        aactggaaa acggcaagg ctagtgttg atccctaag 
        aaaggtatg gacgaagag gggaacagg aaaatgagg 
        ccaatctgc ttgttggat gtattcggc aagttgtta 
        gaacaccta ataagagcg agactgaac gaggacatt 
        gacgacaat gggggcctc tccgaccaa cagtttggc 
        ttcagggag ggacgatgc accctagac gcggttacg 
        gaagtgatg aagttggcc aagtttgca aataacgga 
        acgtgggga cgcaaagat tattgcgca ctggtgacc 
        ctggatatt gaaaatgct tttaactcg gcatcgtgg 
        ctgcacata atagaacaa cttagaaga cgagggata 
        aaaaaggag cttcttaga ctcattatg agttatctg 
        gagggtagg aggatcgaa gtaactcca aaccacatt 
        gtggacatt acctgcgga gtcccccaa ggctcagtt 
        cttgggccg tccctatgg aatatatta tatgatggg 
        gtattaaag caggatatg cccctggga gttaaacta 
        gtagccttc gcagatgac ctggcggtg gctgctata 
        ggacgcaac gaggaagat cttatgagg aacacaaat 
        caggccctc gcacgggta gcaagctgg ttggaagaa 
        aacgaactg aagctggcc gcggaaaaa acggaggct 
        gtgctgctc gttggaaga cgacgaccc agggcggtt 
        cagttccaa ataatgggt gccaagatc gagccaaag 
        aaagtgata aaatacctc ggtatttat atagaccaa 
        gcgtgtact ttttctgac catctcgcc aagaccgca 
        gcaaaagca gaggggaca atatcggca ttgagtcgc 
        attatgcca aacattgga ggacctggg agtaacagg 
        cgaaaactt ctcgcaggg gtggcaact tcaatgctc 
        ctttatggc tgcgaggta tggcacacg gcgctagca 
        acgaaaaag aatgtagtc gtactagaa agcactcat 
        aggaggatc ctggcgcgc gtcgcttgc gcttaccgc 
        acggtttcg acagaagcg gcacaagtc attactgga 
        gtgccgcca gcccggctg ctggtcgaa gaaagggtt 
        aagcaaaga tgtacaggc atgtcaaaa tcaatggcc 
        cgctcacac acgctaaat ctatggcag gaaaactgg 
        cagcagacc accaaagct aggtggacc agaagatta 
        atccctgat atcgagaaa tgggtaaag agaaaacat 
        ggggaaatt gactatcat ctgacgcag gcgctaacg 
        gggcatggg tgcttcggt acctacctg tgccgcatt 
        ggcaaaata ctaaatgca agttgctgg tattgtggc 
        gaggaggac actccgtca catacatta ttttcgtgc 
        tcgtacttc gatccactg aggatggac gtgatgaag 
        aaaattcta gtgtggcca gaagaaact gaaaaagaa 
        aacttgggg ccagtaatt cctgttcag tgctacaga 
        agacgtgct gcggaagca ttaaaaatt gctgagaac 
        actataaaa aatgtcata aaagaaaat ttgggtgaa 
        aaggaggct ccccaaaat actcgaccc aaaaagaag 
        aaaaaaact gaagatgtt tgtaattct gtaaaaatg 
        gatgttaga caaacgata taccagatg attaaagaa 
        aatacctac gtgacaatt aagggactg aaacaagta 
        ttggaggac aaacgcatc taccaaggc agttggtcg 
        agtttgaga aggcttctt ctgtctatt gggtttcat 
        tataaaaga gactgcaat cgacgagct ttgtgtgag 
        aagccacaa attgtttca aaaagatta aattttttg 
        aggaaatac aaggagtat aaggccatg aatttatac 
        gattttgta tttctcgac gagacatgg atctttgcc 
        aaaggagga aatcgtcat tcttggcaa gatgattct 
        atcaaatcg tgccgaagt gctaacgtg gggagtgta 
        gtcaagggc cacaaaacg tcggctgaa gccgaggta 
        ccggcaaga acgacgagc cgggtcggc aacgacagg 
        ggccagtta gacgccata ctgacgaca gcagttctc 
        gcgatgacc gttagtgac cgggagaaa atgtcggtg 
        gaggagcaa cagagacaa actatcgcc aacttggaa 
        cagcagttg gccgaagcc cgagccgca caagccgca 
        cttcagtcg gaccgaaca ggaacacgc gacatagac 
        gctaacata gaagagatc aagaatacc aacttaaag 
        ttgccgtcg ttttggcag agagacccc gcgctgtgg 
        tttgcccaa gtggaggcg cagttccac gcgtatcga 
        atcaaagca gaccagagc agatattat acggtcgtc 
        gcagcgctc gactgcagc gtgctccag gcagtctcc 
        gatattttg acaaaccct cctgagaca aacaaatac 
        gaaaccatc aagacaaaa ttaatatcg gcgtacagc 
        gactcgcag gagaaacag ctccggagg cttttgaat 
        gaactggag ttgggcgac cgaaaacca tcccagttg 
        ctacgggag atgagaacc ctagcagga gtacaggtg 
        aacgaggaa gttctccgt actttgtgg ttacaacgc 
        ctaccggcc caaattcat ctttggctt tcggcgagc 
        gaaagcacc ggcattgcc gtctcgaac tccaggccg 
        tcagagatg atggctccc gactaa
      mRNA sequence
      • >TC031763 RA
        gagcgggcg gacggagaa gtgctggca gtagctcta 
        aaatgcaat cggattgga acttataat gagcggcaa 
        agaacacag gaaagttgt tactgatca gggggtagg 
        atccccaga tccggcagg ggtggagtt gttagcgcg 
        gggctcccc caggtcgtc atccagcga ccgcgatgc 
        agtgcggct agggaacca gggcacact gcaggaaat 
        agcgactgg aacggtcaa gaaacgtgg gaaagatga 
        aggaaaacc gactggcac aacacttgt gcatagccg 
        acttaactt tatggtagg aaacataca gagaagtaa 
        ctctcttag tcacgcaga aaagaaaag gaagtatat 
        ggatacttc agcggagaa cctcaatcc agaggatgg 
        tgtatatat taagaaaga atgcccaga agtattcgt 
        actgaagga ggaactcaa ccagattgt cagactggt 
        gaaagaact tgagaaaaa aaccggaga aatgtataa 
        cccaaaaaa agaactggt taatctagt tagcaaact 
        ttcccgaag cgttcaggt tttgaacag aacttcaat 
        cctgacatg gctggaaaa ccataggta tgaacccgt 
        cgaaaagat gacgataga tgcggatgt gcaaacaga 
        cgcagagag cagcggaga tgaagtaca gactaagac 
        accaaaagc tacgatggt agactgcgc cacacaaat 
        agaaaattg gagtcaaag aaggcccga acttacgac 
        ttggacgca agtaacaaa ttttaagga gtttcagga 
        aatatcaaa aaaggagtg gccatctaa catatatag 
        cagcacgga aatcaaaat cggaaatcc tctagcgac 
        aaaagacag cgtagtaaa ggtggtttt tgtagaacc 
        agaagatac acaaatgac gcgaagtat acagtcact 
        atataaaga tagatatcc agaacttct tgaaatcga 
        agaagatta tggtaccct ggaacaagt tacaaagat 
        aggaaatat ggaaagccg ccgaaaagt tatcaagat 
        tcttgccac aaatcctga agacttgtg ggaaaaatt 
        gaataggtt aaaagaaaa tacagataa cggggaatg 
        gatcgttat gcaccatct tacttggat ggataataa 
        gcagctcca gaaagttgt ggaagcagt ttttcatga 
        aaccgaaac aaaggtggt agtgcaatc gggtggatg 
        gtattgtga tctccgaat cgacgctgc tgtcaaagt 
        tttatcgag ggcggtcag cgtaatcga ctgccggtc 
        aggaaatgg ctttgtttg gatcgaaac ggataagta 
        taagatcta cagttgtta catatcccc caattgtgc 
        ttacgagga atatcatga gactctcga aagactacg 
        ggatcactt agtagtcaa ccgtaaccg ctctcttgt 
        aaccggtga cttcaatgc caaatcgta tttatgggg 
        aaacgtgag cgaagataa aagaggcaa ggagttagc 
        ggatctaat cggtgaact agagatgtg ggtgctaaa 
        tcagggcaa tcagccgac tttcgtcag aggacaaag 
        ctcatcaat aattgatat aacttttgc ttccccaaa 
        ggtagctca atgtgtgaa gggttggca tgttcacga 
        ggatgcccc tctaggtga tcacttgcc gattgtttt 
        cgctctaca gccccagag aaaccgcgc aaacgaagg 
        agaacctcc aagcagatg gcgatggtc cgaaacatg 
        ccgggagag actatttgc ctccctaag aaacaaatt 
        aaaatcaca tatggcgat atccccaga gaaattaac 
        ctctttggt gatagaagc ctgcaagga gaatctcac 
        gagatacga aagcggggg aggtagcag acgcaaggt 
        ctactggtg gaacgacca aataagaag cagtaggac 
        aaaatgcgt gaaggccag aagaactct tatgcgcgc 
        taacagaag gaaacggaa agaagcaat agagttgat 
        gactctaga agccgatta cgctatcgc taaaaaaga 
        gcttcggaa gcaaattaa aattgccaa ggacaatgc 
        ctggaaaaa gcttattga cgagttgga agaggatcc 
        atggggaaa aacatacca agttgtaat gaaaaagct 
        aaaaagaga aacgccccc aactctgaa cgagaagtt 
        gcgaaacgt taaggcact attccccca aaaaattcc 
        aatcttatg ggagccacc gatggttca gacatggga 
        tccgtttac tagggaaga gttggcaac agcggcggc 
        taaaattag ggttaaaaa agcctccgg cccggatgg 
        gataccacc agaggtcgt gcgccttgc atgcgaggc 
        agtacctga ggtggtcct cgggacaat gaatcggat 
        catgaaatc gggagtttt ccctccgaa ctggaaaac 
        ggcaaggct agtgttgat ccctaagaa aggtatgga 
        cgaagaggg gaacaggaa aatgaggcc aatctgctt 
        gttggatgt attcggcaa gttgttaga acacctaat 
        aagagcgag actgaacga ggacattga cgacaatgg 
        gggcctctc cgaccaaca gtttggctt cagggaggg 
        acgatgcac cctagacgc ggttacgga agtgatgaa 
        gttggccaa gtttgcaaa taacggaac gtggggacg 
        caaagatta ttgcgcact ggtgaccct ggatattga 
        aaatgcttt taactcggc atcgtggct gcacataat 
        agaacaact tagaagacg agggataaa aaaggagct 
        tcttagact cattatgag ttatctgga gggtaggag 
        gatcgaagt aactccaaa ccacattgt ggacattac 
        ctgcggagt cccccaagg ctcagttct tgggccgtc 
        cctatggaa tatattata tgatggggt attaaagca 
        ggatatgcc cctgggagt taaactagt agccttcgc 
        agatgacct ggcggtggc tgctatagg acgcaacga 
        ggaagatct tatgaggaa cacaaatca ggccctcgc 
        acgggtagc aagctggtt ggaagaaaa cgaactgaa 
        gctggccgc ggaaaaaac ggaggctgt gctgctcgt 
        tggaagacg acgacccag ggcggttca gttccaaat 
        aatgggtgc caagatcga gccaaagaa agtgataaa 
        atacctcgg tatttatat agaccaagc gtgtacttt 
        ttctgacca tctcgccaa gaccgcagc aaaagcaga 
        ggggacaat atcggcatt gagtcgcat tatgccaaa 
        cattggagg acctgggag taacaggcg aaaacttct 
        cgcaggggt ggcaacttc aatgctcct ttatggctg 
        cgaggtatg gcacacggc gctagcaac gaaaaagaa 
        tgtagtcgt actagaaag cactcatag gaggatcct 
        ggcgcgcgt cgcttgcgc ttaccgcac ggtttcgac 
        agaagcggc acaagtcat tactggagt gccgccagc 
        ccggctgct ggtcgaaga aagggttaa gcaaagatg 
        tacaggcat gtcaaaatc aatggcccg ctcacacac 
        gctaaatct atggcagga aaactggca gcagaccac 
        caaagctag gtggaccag aagattaat ccctgatat 
        cgagaaatg ggtaaagag aaaacatgg ggaaattga 
        ctatcatct gacgcaggc gctaacggg gcatgggtg 
        cttcggtac ctacctgtg ccgcattgg caaaatact 
        aaatgcaag ttgctggta ttgtggcga ggaggacac 
        tccgtcaca tacattatt ttcgtgctc gtacttcga 
        tccactgag gatggacgt gatgaagaa aattctagt 
        gtggccaga agaaactga aaaagaaaa cttggggcc 
        agtaattcc tgttcagtg ctacagaag acgtgctgc 
        ggaagcatt aaaaattgc tgagaacac tataaaaaa 
        tgtcataaa agaaaattt gggtgaaaa ggaggctcc 
        ccaaaatac tcgacccaa aaagaagaa aaaaactga 
        agatgtttg taattctgt aaaaatgga tgttagaca 
        aacgatata ccagatgat taaagaaaa tacctacgt 
        gacaattaa gggactgaa acaagtatt ggaggacaa 
        acgcatcta ccaaggcag ttggtcgag tttgagaag 
        gcttcttct gtctattgg gtttcatta taaaagaga 
        ctgcaatcg acgagcttt gtgtgagaa gccacaaat 
        tgtttcaaa aagattaaa ttttttgag gaaatacaa 
        ggagtataa ggccatgaa tttatacga ttttgtatt 
        tctcgacga gacatggat ctttgccaa aggaggaaa 
        tcgtcattc ttggcaaga tgattctat caaatcgtg 
        ccgaagtgc taacgtggg gagtgtagt caagggcca 
        caaaacgtc ggctgaagc cgaggtacc ggcaagaac 
        gacgagccg ggtcggcaa cgacagggg ccagttaga 
        cgccatact gacgacagc agttctcgc gatgaccgt 
        tagtgaccg ggagaaaat gtcggtgga ggagcaaca 
        gagacaaac tatcgccaa cttggaaca gcagttggc 
        cgaagcccg agccgcaca agccgcact tcagtcgga 
        ccgaacagg aacacgcga catagacgc taacataga 
        agagatcaa gaataccaa cttaaagtt gccgtcgtt 
        ttggcagag agaccccgc gctgtggtt tgcccaagt 
        ggaggcgca gttccacgc gtatcgaat caaagcaga 
        ccagagcag atattatac ggtcgtcgc agcgctcga 
        ctgcagcgt gctccaggc agtctccga tattttgac 
        aaaccctcc tgagacaaa caaatacga aaccatcaa 
        gacaaaatt aatatcggc gtacagcga ctcgcagga 
        gaaacagct ccggaggct tttgaatga actggagtt 
        gggcgaccg aaaaccatc ccagttgct acgggagat 
        gagaaccct agcaggagt acaggtgaa cgaggaagt 
        tctccgtac tttgtggtt acaacgcct accggccca 
        aattcatct ttggctttc ggcgagcga aagcaccgg 
        cattgccgt ctcgaactc caggccgtc agagatgat 
        ggctcccga ctaacctta ccaagacag agactaacc 
        cttaaaagt gatgcttat ccatcactc ttccctgac 
        cttcctgct tatctttcc gaaaatgtt cctgtcaaa 
        cgaaaaaat ccagccaag agattctgt tccctgaat 
        gacgttcac catgaaacg cgggttgaa aaatgtgat 
        gaaatctta aatgaaatt gaaagttta aatgaaatt 
        attgccaag ttacagtgc aaagaatta atatttaat 
        aattggatt gtggaaatg aaagaaagc agcatttat 
        tttttcaaa attgatttt aacgcaatc ccagaaatt 
        gttaaaact atagtactt aatcataac ttaactgtt 
        acaataata tttaaaaaa gttacactg caaacaaaa 
        aaatatcag tatctatta ggaccaaat ttattatgt 
        gactcatac agtaaatta cgagattta gttaatgct 
        cttgataat ttaagtgag gtagaagta acccggtcg 
        gaagtagtt gatacggta tcaagccta attacagag 
        tatgttgga aaaggtcag acagaacaa caaaatagc 
        aaaacaaaa tttttcatt gaacagcta aattttctt 
        aagtcagaa aaacattgc tattccaca gattttcta 
        ctattttgc tgcacattt tcgtttact ttccccgct 
        gcttacaaa tttttaaga gagcaaaat gtaatcaca 
        ttacctaat ccaacatat ctcaaaact tttaatatt 
        aatagtaat ttaggttcc ggcattaat gagtcatta 
        cagatgtat ttaaggaaa aagtcagaa aatttaaac 
        gaccatgaa cgtctggtg tctctttac atagacgaa 
        atacacgtt aaacctaaa ataacatac gcagcggga 
        aaaattgta gggttagct gaaaacagt gataatcta 
        gatgctgct accacaatt caagcattt atgtttaag 
        tctgtatta agtaaaaac aaagaaatg gtagcgtta 
        tttccagtg aaaaagtta gatgcagat tatttgcat 
        gaaactact attaaagtt ttacaattg cttcacagt 
        tccagtttt agagttgta tcaattact tccgacaat 
        aacaaagtt aatgtaaaa ttatttaaa aaattatct 
        aacggaaat atggcccct tatatattg aatcctaat 
        aatgaaaat gaaagaatt tacttactt tttgataca 
        gttcacttg ttaaaaaat atacgtaat aattggctt 
        aatgaaaaa acaaaaacc ttttttact ccttcattt 
        tcagatcac tcttctata atatgctgc tcatttaca 
        aacataatt aatttatat gaatctgaa aaattctct 
        gttgtaaaa aaagctccc ggtctttca tataaagtt 
        tgttatcct aacaactta gaacgacaa aatgttaat 
        cttgcatta caaattttt aatgaaaaa accatagca 
        ggtgtaaca gaccaaggt acaaaacaa tttttaacc 
        attgttaat cgatggtgg agggtagtt aacgtaaaa 
        caccctagc aaaggcaaa cacttgcgg gacatttat 
        gccaatcca atcattagt tcatgtagt ctaggtctt 
        caattcttg caggccttt gtgtcctgg cttaaaaaa 
        tgggaagat attgtaatt gtaggcgtc cctaaaaaa 
        gcccataaa cttagcaaa gaaacacat ttcgcttta 
        acatttacc acaagtaat ttgatattg ttaagtgaa 
        tatctttta tataactta aatttaaaa tatgtattg 
        ttaggaaat ctacaaaca gatgtatta gaacgtcga 
        tttgggcaa tatcggaaa cttagtggg tgcaattac 
        aacgtgtct gtaaaacaa attcttgag tctgaaaaa 
        aaaatcaaa gttaaaagt ttgttgcgg ttaaacacc 
        aataaaatt cagataaag ga
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