Status
Please wait ...
Gene information
General information
TC010267 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 28564213..28577124
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010267
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04802

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010267 RA
      MTNIYVKKI LDNQETARK AAKEKCISM FGPGVLQEF 
      AEPVYNETN KTLKCPAFW DTILCWPDT PSGTVINQS 
      CPVYVAGFL ASSNATRQC MENGSWYIR DNRTWSNYS 
      SCYKDKITT IYLDLNKTN ISGVLQNYA PVVQVISET 
      GYIVSFATL IIAFAIMLF IKKLHCARN ILHMHLFAS 
      FILRALTFI VIKSTFVEG LGLPSDLNY RNGSLYFDI 
      NSETNNWAC KLLTSLWQY FITANYSWI LMEGLYLHN 
      LIFRALFAD SSNSIKWYV VMGWGLPLI IVGFWVAAR 
      LLVEDNLCW TTHENYDVF LIIGIPTMV SILINLLLF 
      MRISMVLYS KLRSPINED SRRYQKWVK STLVLVPLF 
      GVHYALFLA LYYLIKTNK IVEVVWLFC DLLFGSFQG 
      FFVAILYCF LNGEVKSEI QPHLYYFLT YLATNKYSK 
      CLFPCRKKF LRSVVGRSS VCTTMSCSS LYTNGVLHR 
      NSKCRLDSL PKIKTTDKP KDHFCNNTS RRNSRSQRH 
      SQAFNSSAT IPTAETTLC IENNSGKCR SESEINEES 
      LKMVVHSDF
    CDS sequence
    • >TC010267 RA
      atgaccaac atctacgtc aagaaaatc ctggataat 
      caggagacg gcgcgaaag gcggccaag gaaaaatgc 
      atttcgatg ttcggtcca ggagtcttg caagaattt 
      gcggaacca gtttacaac gagaccaac aaaactctg 
      aaatgccca gccttttgg gacacgatc ctttgttgg 
      cccgatact ccctctggt actgtcata aatcagtcg 
      tgtccggtc tatgtcgct ggttttctt gcgtccagt 
      aatgcgacg cgtcaatgt atggaaaat ggcagttgg 
      tatattagg gataatagg acgtggagc aattatagc 
      agttgctac aaggataaa atcaccacg atttacctc 
      gatttgaac aagaccaac atatcaggg gttctacag 
      aattatgcc ccagtcgtc caagtcata tcagaaacg 
      ggatatatt gtctcgttt gccactctt atcatcgcc 
      tttgctatc atgttgttt atcaagaaa ctccattgt 
      gccagaaac attctccac atgcacctc ttcgcctcc 
      ttcattctc cgagccctt acgttcatc gtaatcaaa 
      agcactttc gtcgaaggg cttggttta ccttcagat 
      ctcaattac cgaaacggc tctctatac ttcgacatc 
      aattccgag accaataac tgggcttgc aaactcctc 
      acgagtctg tggcagtac ttcatcaca gctaattac 
      tcgtggatt ttgatggaa gggctgtat ttgcataat 
      ctgatcttc agggcgctt tttgcagac tcgagtaac 
      agcattaag tggtatgtt gtgatgggc tgggggctg 
      cctttgatt atcgtgggg ttctgggtc gctgcaaga 
      cttctcgtt gaagataat ctctgttgg accacgcat 
      gaaaattac gacgtgttt ttgatcatt ggaataccc 
      accatggtg tcaattttg atcaatttg ttactgttt 
      atgcggatc tccatggta ctttacagc aaattacga 
      tcaccaatt aatgaagac tccagaagg taccaaaag 
      tgggtcaag tcaacccta gtcctagta cctttattt 
      ggcgtccac tacgcacta ttcctagca ctgtactat 
      ttgattaaa accaacaaa atcgtagaa gtggtttgg 
      cttttctgc gacttgttg tttggcagt ttccagggc 
      ttctttgtg gccatcctt tactgcttc ctcaacggc 
      gaagtgaaa tcagagatc cagccccac ctctactac 
      ttcttgact tacctcgcc actaacaaa tactccaag 
      tgcttgttt ccttgccga aagaagttc ctaagatca 
      gttgtgggc aggtcctca gtttgcacg actatgtct 
      tgtagctct ctctacacc aacggcgtt ttgcaccgc 
      aacagcaaa tgccggctg gattctctt ccaaaaatc 
      aagaccaca gataagcca aaagaccac ttttgtaac 
      aacacttcg aggaggaat tccaggtcg cagcgacat 
      tcccaagct ttcaactcg tctgcaacc attcctaca 
      gcagagacg acactttgc atcgaaaac aactcaggg 
      aaatgtcgc tccgaatcg gaaatcaac gaagaaagt 
      ctcaaaatg gtagtgcac agtgacttc taa
    mRNA sequence
    • >TC010267 RA
      catgatttc gagatttgt cttatcaca gataaatac 
      agctgaact agtgatggt ttaagaata gtgtgatgg 
      ttcgttcga ataaaacca agtggtttg tgacatgtg 
      tcactcgtt aattggctg aagtgagtc gcgtcgaaa 
      attatggag ttccaagac taaaaatcg accacgatg 
      accaacatc tacgtcaag aaaatcctg gataatcag 
      gagacggcg cgaaaggcg gccaaggaa aaatgcatt 
      tcgatgttc ggtccagga gtcttgcaa gaatttgcg 
      gaaccagtt tacaacgag accaacaaa actctgaaa 
      tgcccagcc ttttgggac acgatcctt tgttggccc 
      gatactccc tctggtact gtcataaat cagtcgtgt 
      ccggtctat gtcgctggt tttcttgcg tccagtaat 
      gcgacgcgt caatgtatg gaaaatggc agttggtat 
      attagggat aataggacg tggagcaat tatagcagt 
      tgctacaag gataaaatc accacgatt tacctcgat 
      ttgaacaag accaacata tcaggggtt ctacagaat 
      tatgcccca gtcgtccaa gtcatatca gaaacggga 
      tatattgtc tcgtttgcc actcttatc atcgccttt 
      gctatcatg ttgtttatc aagaaactc cattgtgcc 
      agaaacatt ctccacatg cacctcttc gcctccttc 
      attctccga gcccttacg ttcatcgta atcaaaagc 
      actttcgtc gaagggctt ggtttacct tcagatctc 
      aattaccga aacggctct ctatacttc gacatcaat 
      tccgagacc aataactgg gcttgcaaa ctcctcacg 
      agtctgtgg cagtacttc atcacagct aattactcg 
      tggattttg atggaaggg ctgtatttg cataatctg 
      atcttcagg gcgcttttt gcagactcg agtaacagc 
      attaagtgg tatgttgtg atgggctgg gggctgcct 
      ttgattatc gtggggttc tgggtcgct gcaagactt 
      ctcgttgaa gataatctc tgttggacc acgcatgaa 
      aattacgac gtgtttttg atcattgga atacccacc 
      atggtgtca attttgatc aatttgtta ctgtttatg 
      cggatctcc atggtactt tacagcaaa ttacgatca 
      ccaattaat gaagactcc agaaggtac caaaagtgg 
      gtcaagtca accctagtc ctagtacct ttatttggc 
      gtccactac gcactattc ctagcactg tactatttg 
      attaaaacc aacaaaatc gtagaagtg gtttggctt 
      ttctgcgac ttgttgttt ggcagtttc cagggcttc 
      tttgtggcc atcctttac tgcttcctc aacggcgaa 
      gtgaaatca gagatccag ccccacctc tactacttc 
      ttgacttac ctcgccact aacaaatac tccaagtgc 
      ttgtttcct tgccgaaag aagttccta agatcagtt 
      gtgggcagg tcctcagtt tgcacgact atgtcttgt 
      agctctctc tacaccaac ggcgttttg caccgcaac 
      agcaaatgc cggctggat tctcttcca aaaatcaag 
      accacagat aagccaaaa gaccacttt tgtaacaac 
      acttcgagg aggaattcc aggtcgcag cgacattcc 
      caagctttc aactcgtct gcaaccatt cctacagca 
      gagacgaca ctttgcatc gaaaacaac tcagggaaa 
      tgtcgctcc gaatcggaa atcaacgaa gaaagtctc 
      aaaatggta gtgcacagt gacttctaa cttgaagtt 
      gtttctttt ttgtgatat ctgtcgatg tcaatcaat 
      ttatttgat gtaatatga ggtggttct agtcggttt 
      gacgttccg aacggagga acgtgtgta tttttttaa 
      acgtggatg taagactgc taaaatgtt aaatgtgat 
      gttcaaggt acacttttg tcacatgta tgattacaa 
      tttttgaat aaaatattt catcttac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY