Status
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Gene information
General information
TC031668
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010256,TC010257
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450712.1: 164451..173482
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031668
    iBeetle screen iB_04800

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head appendages partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax segment partiallynot present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs pretarsus duplicated - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs tibiotarsus shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031668 RA
      MFSVLVFLI IVVVFLYYK WAFTYWHRR NIPYLEPSF 
      PFGNLPNLF QRKENIGLT VEKFYNEMK RRKWKHGGV 
      YFLVSPVYC VIDLEYVKN IMNRDFEYF TNRSIYYNE 
      KDDPLSAHL FAIGGQKWR NLRTKLTAT FTSGKMKAM 
      FQTLLECET NLLKQIEDC NNPINIKEI LSCFTTDVI 
      GSVGFGLEC KTFEEKNSP FRIYGKKVF KDSVLRSLK 
      RAFLTSNFP ELARKLGIL AFPSEITDF FLKVVADTV 
      KYREENNRS RNDFLQLLI DLKNSGTLS LEEIAAQCF 
      LFFLAGFET SSTTMTFAL YELAQHQDI QDKVREEID 
      AVLKKYGGK ITYEAIHDM KYMNQVIDE TLRKYPAAS 
      IITRTCVKD YKIPDQDIV IEKGTSVII PVLGIHHDE 
      KFYPNPEKF DPERFTEEN KAARHHYAH LPFGEGPRI 
      CIDYQFERV SSCYTVKMV LSLCRWEGK VAVVTGASS 
      GIGAAIADA LVENGLIVA GFARRSELI EARAKNLEG 
      KKGKLHAVK TDMSKEDEI IKAFKWVED NLGHVHILI 
      NNAGVSKES FLANGDAEA WRNTFDVNV IGLCIATRE 
      AVKSMTANN INGHIIHIN SIAGHKVVN FPGINVYSA 
      SKYAVAALA ETLRHEFLA LGSKIKITS ISPGLVISE 
      MTTLSTGYS PERLEMLKS LPILKSEDI ADAVCYVLS 
      TPETVQINE LTITPVGEK L
    CDS sequence
    • >TC031668 RA
      atgttttcg gttttggtg tttctaatt atagttgta 
      gtgtttctt tattacaaa tgggcgttc acatactgg 
      caccgaaga aacatacca tatttagag cccagtttt 
      ccctttggc aatttaccc aaccttttc caacgcaag 
      gaaaatata ggacttacg gtagaaaaa ttctacaac 
      gaaatgaaa aggcgcaag tggaaacac ggaggagtt 
      tactttctt gtaagtcca gtctattgt gtcattgat 
      ttggaatat gtaaagaac ataatgaat cgagatttc 
      gaatatttt accaaccgc tcgatttat tacaacgag 
      aaagacgac cctcttagt gcacattta tttgcaatc 
      ggtggccaa aaatggcga aacttgcga actaaatta 
      acagcaact ttcacatca ggtaaaatg aaagcaatg 
      tttcaaaca ctgctagaa tgtgaaacc aatttattg 
      aaacaaatc gaagactgt aacaaccca attaacatt 
      aaagaaatt ttaagttgc tttactact gacgtcatc 
      ggttcggtc ggttttggt ctcgaatgc aagactttc 
      gaagaaaaa aattcaccg tttcgcatt tatggcaaa 
      aaagtcttc aaggattca gtgttgaga agtttgaaa 
      cgcgcgttt ctcacatcg aattttcca gagttagct 
      cggaaactc ggaatttta gcgtttcca tcggagata 
      accgatttt ttcctcaaa gttgtagct gatactgtt 
      aagtacaga gaggaaaac aaccgtagc aggaatgat 
      ttcttgcaa cttttgatt gatttgaag aatagtgga 
      actttgtca cttgaagaa attgccgct caatgtttt 
      cttttcttt ttggcgggt tttgaaacg tcttcaacc 
      acaatgact tttgctttg tacgaattg gcccaacat 
      caagacatt caagataaa gtgagggaa gaaattgat 
      gcagttttg aagaaatat ggtggcaaa atcacttat 
      gaggccatt catgacatg aaatacatg aatcaagtt 
      attgatgaa acgttaaga aaataccca gcagcttca 
      atcatcacc aggacttgt gtcaaagat tacaaaatt 
      cctgatcag gatattgtg attgaaaaa gggacttcg 
      gtcataatc ccagttttg ggtatccac catgatgag 
      aaattttat ccaaatcct gaaaaattt gatcctgaa 
      agatttact gaagaaaat aaagctgca agacatcat 
      tatgcgcat ctacctttt ggagaaggg ccacgaatt 
      tgtatcgat tatcaattc gagcgagta tcatcttgt 
      tacacagtc aaaatggtt ctttcacta tgcagatgg 
      gagggcaaa gtggctgta gtgaccggt gcaagttca 
      ggaatcggt gctgcaatt gctgacgct ttggtagaa 
      aacggtctc atcgttgct ggatttgct cgtcgctct 
      gaactgatc gaagcgcgg gccaaaaat ctcgaagga 
      aaaaaaggg aaactccac gctgttaaa actgacatg 
      agcaaagaa gatgaaatc atcaaagct ttcaaatgg 
      gttgaagac aacctcgga catgtccac atcttaata 
      aacaatgct ggtgtttct aaggaaagt ttcttagca 
      aacggagat gctgaggct tggaggaac acttttgat 
      gttaacgta ataggatta tgtattgcc acaagagaa 
      gctgtgaaa tcaatgaca gcaaacaac ataaatggg 
      catattatt cacattaat agcattgca gggcataaa 
      gttgtgaat tttcccggg attaatgtc tactcagcc 
      agcaaatat gcagtcgca gctttagct gagactttg 
      aggcacgag tttctcgct ctggggtcg aaaatcaaa 
      attacgagc attagtcct ggattggtt ataagtgaa 
      atgacaact cttagtaca ggatacagt cccgaacga 
      ttggaaatg ctgaaaagt ctaccaata ctaaagtct 
      gaagacatt gcagatgca gtatgctat gtattgtcc 
      acacctgaa actgtccag ataaacgaa ctgacaatc 
      actccagtc ggcgaaaaa ctttga
    mRNA sequence
    • >TC031668 RA
      ctgttgaga aattacaag tttacagtt aataacatg 
      actagagaa cccctgaga atgcaagtg gactcgttt 
      gttttggca gccgaaggg gaaatttgg ttagatgca 
      cacaagttg tgaaaattg ctgtaaaaa attgagcaa 
      taaaaatta aattctagt gaggtgttg agagattct 
      cagttctaa tcatatcat ttatttggc gtatcagcg 
      accactcaa agcgcatct aaaaataaa acttgaaaa 
      ctgtgtaga taataattc taaagaaaa aatatgacg 
      tttattatc atgtcacaa cataaaaat cactatcac 
      acttttaag cgatgtgtc taatcaaca atgatgttg 
      caagtagtt cacgatata aatagcttc acactttca 
      ttgatacat gtattcaat ctcagaaaa aatgttttc 
      ggttttggt gtttctaat tatagttgt agtgtttct 
      ttattacaa atgggcgtt cacatactg gcaccgaag 
      aaacatacc atatttaga gcccagttt tccctttgg 
      caatttacc caacctttt ccaacgcaa ggaaaatat 
      aggacttac ggtagaaaa attctacaa cgaaatgaa 
      aaggcgcaa gtggaaaca cggaggagt ttactttct 
      tgtaagtcc agtctattg tgtcattga tttggaata 
      tgtaaagaa cataatgaa tcgagattt cgaatattt 
      taccaaccg ctcgattta ttacaacga gaaagacga 
      ccctcttag tgcacattt atttgcaat cggtggcca 
      aaaatggcg aaacttgcg aactaaatt aacagcaac 
      tttcacatc aggtaaaat gaaagcaat gtttcaaac 
      actgctaga atgtgaaac caatttatt gaaacaaat 
      cgaagactg taacaaccc aattaacat taaagaaat 
      tttaagttg ctttactac tgacgtcat cggttcggt 
      cggttttgg tctcgaatg caagacttt cgaagaaaa 
      aaattcacc gtttcgcat ttatggcaa aaaagtctt 
      caaggattc agtgttgag aagtttgaa acgcgcgtt 
      tctcacatc gaattttcc agagttagc tcggaaact 
      cggaatttt agcgtttcc atcggagat aaccgattt 
      tttcctcaa agttgtagc tgatactgt taagtacag 
      agaggaaaa caaccgtag caggaatga tttcttgca 
      acttttgat tgatttgaa gaatagtgg aactttgtc 
      acttgaaga aattgccgc tcaatgttt tcttttctt 
      tttggcggg ttttgaaac gtcttcaac cacaatgac 
      ttttgcttt gtacgaatt ggcccaaca tcaagacat 
      tcaagataa agtgaggga agaaattga tgcagtttt 
      gaagaaata tggtggcaa aatcactta tgaggccat 
      tcatgacat gaaatacat gaatcaagt tattgatga 
      aacgttaag aaaataccc agcagcttc aatcatcac 
      caggacttg tgtcaaaga ttacaaaat tcctgatca 
      ggatattgt gattgaaaa agggacttc ggtcataat 
      cccagtttt gggtatcca ccatgatga gaaatttta 
      tccaaatcc tgaaaaatt tgatcctga aagatttac 
      tgaagaaaa taaagctgc aagacatca ttatgcgca 
      tctaccttt tggagaagg gccacgaat ttgtatcga 
      ttatcaatt cgagcgagt atcatcttg ttacacagt 
      caaaatggt tctttcact atgcagatg ggagggcaa 
      agtggctgt agtgaccgg tgcaagttc aggaatcgg 
      tgctgcaat tgctgacgc tttggtaga aaacggtct 
      catcgttgc tggatttgc tcgtcgctc tgaactgat 
      cgaagcgcg ggccaaaaa tctcgaagg aaaaaaagg 
      gaaactcca cgctgttaa aactgacat gagcaaaga 
      agatgaaat catcaaagc tttcaaatg ggttgaaga 
      caacctcgg acatgtcca catcttaat aaacaatgc 
      tggtgtttc taaggaaag tttcttagc aaacggaga 
      tgctgaggc ttggaggaa cacttttga tgttaacgt 
      aataggatt atgtattgc cacaagaga agctgtgaa 
      atcaatgac agcaaacaa cataaatgg gcatattat 
      tcacattaa tagcattgc agggcataa agttgtgaa 
      ttttcccgg gattaatgt ctactcagc cagcaaata 
      tgcagtcgc agctttagc tgagacttt gaggcacga 
      gtttctcgc tctggggtc gaaaatcaa aattacgag 
      cattagtcc tggattggt tataagtga aatgacaac 
      tcttagtac aggatacag tcccgaacg attggaaat 
      gctgaaaag tctaccaat actaaagtc tgaagacat 
      tgcagatgc agtatgcta tgtattgtc cacacctga 
      aactgtcca gataaacga actgacaat cactccagt 
      cggcgaaaa actttgaag aatgtttta attttttaa 
      cagtacgat tcacataac actacgagc ctacattta 
      catctgcat ttgcccgtc agtttcaca gtgaatgac 
      tctgtacag gtgtctgga aagtcgtgc tcctcatta 
      aaggggagt aattgggta gtcccttag ggtatcaaa 
      aaaatgttt aaaagaaat acacatgct ccatcaga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY