GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_04800 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head appendages partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax segment partiallynot present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
legs pretarsus duplicated - Penetrance (percentage of animals/eggs on slide): less than 30%
legs tibiotarsus shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
- >TC031668 RA
MFSVLVFLI IVVVFLYYK WAFTYWHRR NIPYLEPSF PFGNLPNLF QRKENIGLT VEKFYNEMK RRKWKHGGV YFLVSPVYC VIDLEYVKN IMNRDFEYF TNRSIYYNE KDDPLSAHL FAIGGQKWR NLRTKLTAT FTSGKMKAM FQTLLECET NLLKQIEDC NNPINIKEI LSCFTTDVI GSVGFGLEC KTFEEKNSP FRIYGKKVF KDSVLRSLK RAFLTSNFP ELARKLGIL AFPSEITDF FLKVVADTV KYREENNRS RNDFLQLLI DLKNSGTLS LEEIAAQCF LFFLAGFET SSTTMTFAL YELAQHQDI QDKVREEID AVLKKYGGK ITYEAIHDM KYMNQVIDE TLRKYPAAS IITRTCVKD YKIPDQDIV IEKGTSVII PVLGIHHDE KFYPNPEKF DPERFTEEN KAARHHYAH LPFGEGPRI CIDYQFERV SSCYTVKMV LSLCRWEGK VAVVTGASS GIGAAIADA LVENGLIVA GFARRSELI EARAKNLEG KKGKLHAVK TDMSKEDEI IKAFKWVED NLGHVHILI NNAGVSKES FLANGDAEA WRNTFDVNV IGLCIATRE AVKSMTANN INGHIIHIN SIAGHKVVN FPGINVYSA SKYAVAALA ETLRHEFLA LGSKIKITS ISPGLVISE MTTLSTGYS PERLEMLKS LPILKSEDI ADAVCYVLS TPETVQINE LTITPVGEK L
- >TC031668 RA
atgttttcg gttttggtg tttctaatt atagttgta gtgtttctt tattacaaa tgggcgttc acatactgg caccgaaga aacatacca tatttagag cccagtttt ccctttggc aatttaccc aaccttttc caacgcaag gaaaatata ggacttacg gtagaaaaa ttctacaac gaaatgaaa aggcgcaag tggaaacac ggaggagtt tactttctt gtaagtcca gtctattgt gtcattgat ttggaatat gtaaagaac ataatgaat cgagatttc gaatatttt accaaccgc tcgatttat tacaacgag aaagacgac cctcttagt gcacattta tttgcaatc ggtggccaa aaatggcga aacttgcga actaaatta acagcaact ttcacatca ggtaaaatg aaagcaatg tttcaaaca ctgctagaa tgtgaaacc aatttattg aaacaaatc gaagactgt aacaaccca attaacatt aaagaaatt ttaagttgc tttactact gacgtcatc ggttcggtc ggttttggt ctcgaatgc aagactttc gaagaaaaa aattcaccg tttcgcatt tatggcaaa aaagtcttc aaggattca gtgttgaga agtttgaaa cgcgcgttt ctcacatcg aattttcca gagttagct cggaaactc ggaatttta gcgtttcca tcggagata accgatttt ttcctcaaa gttgtagct gatactgtt aagtacaga gaggaaaac aaccgtagc aggaatgat ttcttgcaa cttttgatt gatttgaag aatagtgga actttgtca cttgaagaa attgccgct caatgtttt cttttcttt ttggcgggt tttgaaacg tcttcaacc acaatgact tttgctttg tacgaattg gcccaacat caagacatt caagataaa gtgagggaa gaaattgat gcagttttg aagaaatat ggtggcaaa atcacttat gaggccatt catgacatg aaatacatg aatcaagtt attgatgaa acgttaaga aaataccca gcagcttca atcatcacc aggacttgt gtcaaagat tacaaaatt cctgatcag gatattgtg attgaaaaa gggacttcg gtcataatc ccagttttg ggtatccac catgatgag aaattttat ccaaatcct gaaaaattt gatcctgaa agatttact gaagaaaat aaagctgca agacatcat tatgcgcat ctacctttt ggagaaggg ccacgaatt tgtatcgat tatcaattc gagcgagta tcatcttgt tacacagtc aaaatggtt ctttcacta tgcagatgg gagggcaaa gtggctgta gtgaccggt gcaagttca ggaatcggt gctgcaatt gctgacgct ttggtagaa aacggtctc atcgttgct ggatttgct cgtcgctct gaactgatc gaagcgcgg gccaaaaat ctcgaagga aaaaaaggg aaactccac gctgttaaa actgacatg agcaaagaa gatgaaatc atcaaagct ttcaaatgg gttgaagac aacctcgga catgtccac atcttaata aacaatgct ggtgtttct aaggaaagt ttcttagca aacggagat gctgaggct tggaggaac acttttgat gttaacgta ataggatta tgtattgcc acaagagaa gctgtgaaa tcaatgaca gcaaacaac ataaatggg catattatt cacattaat agcattgca gggcataaa gttgtgaat tttcccggg attaatgtc tactcagcc agcaaatat gcagtcgca gctttagct gagactttg aggcacgag tttctcgct ctggggtcg aaaatcaaa attacgagc attagtcct ggattggtt ataagtgaa atgacaact cttagtaca ggatacagt cccgaacga ttggaaatg ctgaaaagt ctaccaata ctaaagtct gaagacatt gcagatgca gtatgctat gtattgtcc acacctgaa actgtccag ataaacgaa ctgacaatc actccagtc ggcgaaaaa ctttga
- >TC031668 RA
ctgttgaga aattacaag tttacagtt aataacatg actagagaa cccctgaga atgcaagtg gactcgttt gttttggca gccgaaggg gaaatttgg ttagatgca cacaagttg tgaaaattg ctgtaaaaa attgagcaa taaaaatta aattctagt gaggtgttg agagattct cagttctaa tcatatcat ttatttggc gtatcagcg accactcaa agcgcatct aaaaataaa acttgaaaa ctgtgtaga taataattc taaagaaaa aatatgacg tttattatc atgtcacaa cataaaaat cactatcac acttttaag cgatgtgtc taatcaaca atgatgttg caagtagtt cacgatata aatagcttc acactttca ttgatacat gtattcaat ctcagaaaa aatgttttc ggttttggt gtttctaat tatagttgt agtgtttct ttattacaa atgggcgtt cacatactg gcaccgaag aaacatacc atatttaga gcccagttt tccctttgg caatttacc caacctttt ccaacgcaa ggaaaatat aggacttac ggtagaaaa attctacaa cgaaatgaa aaggcgcaa gtggaaaca cggaggagt ttactttct tgtaagtcc agtctattg tgtcattga tttggaata tgtaaagaa cataatgaa tcgagattt cgaatattt taccaaccg ctcgattta ttacaacga gaaagacga ccctcttag tgcacattt atttgcaat cggtggcca aaaatggcg aaacttgcg aactaaatt aacagcaac tttcacatc aggtaaaat gaaagcaat gtttcaaac actgctaga atgtgaaac caatttatt gaaacaaat cgaagactg taacaaccc aattaacat taaagaaat tttaagttg ctttactac tgacgtcat cggttcggt cggttttgg tctcgaatg caagacttt cgaagaaaa aaattcacc gtttcgcat ttatggcaa aaaagtctt caaggattc agtgttgag aagtttgaa acgcgcgtt tctcacatc gaattttcc agagttagc tcggaaact cggaatttt agcgtttcc atcggagat aaccgattt tttcctcaa agttgtagc tgatactgt taagtacag agaggaaaa caaccgtag caggaatga tttcttgca acttttgat tgatttgaa gaatagtgg aactttgtc acttgaaga aattgccgc tcaatgttt tcttttctt tttggcggg ttttgaaac gtcttcaac cacaatgac ttttgcttt gtacgaatt ggcccaaca tcaagacat tcaagataa agtgaggga agaaattga tgcagtttt gaagaaata tggtggcaa aatcactta tgaggccat tcatgacat gaaatacat gaatcaagt tattgatga aacgttaag aaaataccc agcagcttc aatcatcac caggacttg tgtcaaaga ttacaaaat tcctgatca ggatattgt gattgaaaa agggacttc ggtcataat cccagtttt gggtatcca ccatgatga gaaatttta tccaaatcc tgaaaaatt tgatcctga aagatttac tgaagaaaa taaagctgc aagacatca ttatgcgca tctaccttt tggagaagg gccacgaat ttgtatcga ttatcaatt cgagcgagt atcatcttg ttacacagt caaaatggt tctttcact atgcagatg ggagggcaa agtggctgt agtgaccgg tgcaagttc aggaatcgg tgctgcaat tgctgacgc tttggtaga aaacggtct catcgttgc tggatttgc tcgtcgctc tgaactgat cgaagcgcg ggccaaaaa tctcgaagg aaaaaaagg gaaactcca cgctgttaa aactgacat gagcaaaga agatgaaat catcaaagc tttcaaatg ggttgaaga caacctcgg acatgtcca catcttaat aaacaatgc tggtgtttc taaggaaag tttcttagc aaacggaga tgctgaggc ttggaggaa cacttttga tgttaacgt aataggatt atgtattgc cacaagaga agctgtgaa atcaatgac agcaaacaa cataaatgg gcatattat tcacattaa tagcattgc agggcataa agttgtgaa ttttcccgg gattaatgt ctactcagc cagcaaata tgcagtcgc agctttagc tgagacttt gaggcacga gtttctcgc tctggggtc gaaaatcaa aattacgag cattagtcc tggattggt tataagtga aatgacaac tcttagtac aggatacag tcccgaacg attggaaat gctgaaaag tctaccaat actaaagtc tgaagacat tgcagatgc agtatgcta tgtattgtc cacacctga aactgtcca gataaacga actgacaat cactccagt cggcgaaaa actttgaag aatgtttta attttttaa cagtacgat tcacataac actacgagc ctacattta catctgcat ttgcccgtc agtttcaca gtgaatgac tctgtacag gtgtctgga aagtcgtgc tcctcatta aaggggagt aattgggta gtcccttag ggtatcaaa aaaatgttt aaaagaaat acacatgct ccatcaga
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
