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Gene information
General information
TC010254 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450712.1: 140121..142632
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010254
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04799

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010254 RA
      MFVSNSLLG DLIALFVTL GVLLTFYFK WTYQYWTRR 
      NVPQIDPHI PFGNISESI KGREIVGVT FKKIYQQLK 
      SQGCRHGGI YIFTQPSYF PIDLDYIRN ILTKDFQYF 
      VDRPFYVNE KDPLQAHLL NLSGTRWRN MRSKLTPTF 
      TSGKMKLMF HIMAESQSD LLKRMDKEA ESLEPINIK 
      EVLACFTTN IIGSCAFGL DCKALEDEN SPFRQFGKK 
      LFKISVLHR LKRTFAATF PDFAKFLRM SNSRKEIVK 
      FIMDMVEGT IEYREKNNY SRNDFMQLL IDMKNNKPK 
      GDHDGKPLT LDEVASQAI VFFAAGFET SSTLMTFAF 
      YELAKNPHI QDKLREEIN LILAEHNNE ISYDAIQEM 
      KYLSQVIDE TLRLHPPAA QTSRKCIKD YKIPDQDIT 
      IEKGTSVTV SILGIHHDP DYYPDPEKF DPERFTEEN 
      KSLRHNYAF LPFGEGPRN CIGMRFGLL QSKMGLVSL 
      IKNYKFTVN EKTKEPITY QPAHVVLAA QGEIWLNAQ 
      KV
    CDS sequence
    • >TC010254 RA
      atgtttgtt tccaactcg ttacttggc gatttgatc 
      gctcttttt gtaactctt ggggtgctc ctaactttt 
      tatttcaaa tggacttac cagtactgg accagacgt 
      aacgtccct caaattgac cctcacata ccttttgga 
      aacatctct gagtcaatc aagggccga gagattgtg 
      ggagtgaca ttcaagaaa atctaccaa caactcaaa 
      agtcaaggg tgcagacat ggtggcatt tacattttc 
      acacaacca tcgtatttt cccattgac ttggattat 
      attcgtaat attttgacg aaagatttt caatatttc 
      gtcgatcgg cccttttat gtcaatgag aaagatcca 
      ctccaagca catctcttg aatttgagt ggcactagg 
      tggcgcaat atgaggtcc aagttgact ccaactttc 
      acctcaggt aagatgaaa ctgatgttc catataatg 
      gcagaaagt cagagcgat ttactgaaa cgaatggat 
      aaagaagct gagtcactt gaaccgatc aatatcaaa 
      gaggttttg gcatgtttt actacaaat atcatcggt 
      tcctgtgct tttggactc gactgtaag gctctggaa 
      gatgaaaat tctccgttt cgtcagttt gggaaaaaa 
      ttatttaag atatcagtt ctccaccgc ttgaaacgc 
      acctttgct gcgactttc cccgacttt gcaaagttt 
      ttacggatg tcaaacagt cggaaagaa atcgtcaag 
      tttattatg gacatggta gaaggtacg attgaatat 
      cgagagaaa aacaattac tccagaaac gattttatg 
      caacttttg atcgacatg aagaacaac aaaccaaag 
      ggcgatcac gatggcaaa cccttgacc cttgacgaa 
      gtcgcatct caagctata gttttcttt gctgcaggt 
      tttgaaact tcgtcaact ctcatgact ttcgccttc 
      tacgaactt gccaaaaac cctcacatc caagacaag 
      ttgagagag gaaattaat ttaattctg gcagaacat 
      aataatgaa attagttat gacgccatc caagaaatg 
      aaatactta agccaagtg attgacgag actttaaga 
      ctgcaccct ccagcagca caaactagt cgcaagtgc 
      attaaagat tacaaaatt ccggaccaa gatattaca 
      atcgagaaa ggaacaagt gttacagtt tcaattctg 
      gggattcat cacgacccg gattattat ccggacccg 
      gaaaaattc gacccagaa cgattcact gaagaaaat 
      aagagttta aggcataat tatgcgttt ttgccgttt 
      ggtgaaggt cccagaaac tgcattgga atgcggttt 
      ggtctcttg cagtcgaag atgggcttg gtttcatta 
      atcaaaaat tataaattt acagtgaat gaaaagacc 
      aaagagcca attacttac cagccagcg catgttgtt 
      ttggctgct cagggagaa atatggctc aatgcccaa 
      aaagtataa
    mRNA sequence
    • >TC010254 RA
      tgtttacaa tattttgtc agcatgagt gcgtgcacc 
      gagaaaata attctatat aaatatgtt ggctcgttt 
      cttaatcta gtgtaatca agccatgtt tgtttccaa 
      ctcgttact tggcgattt gatcgctct ttttgtaac 
      tcttggggt gctcctaac tttttattt caaatggac 
      ttaccagta ctggaccag acgtaacgt ccctcaaat 
      tgaccctca cataccttt tggaaacat ctctgagtc 
      aatcaaggg ccgagagat tgtgggagt gacattcaa 
      gaaaatcta ccaacaact caaaagtca agggtgcag 
      acatggtgg catttacat tttcacaca accatcgta 
      ttttcccat tgacttgga ttatattcg taatatttt 
      gacgaaaga ttttcaata tttcgtcga tcggccctt 
      ttatgtcaa tgagaaaga tccactcca agcacatct 
      cttgaattt gagtggcac taggtggcg caatatgag 
      gtccaagtt gactccaac tttcacctc aggtaagat 
      gaaactgat gttccatat aatggcaga aagtcagag 
      cgatttact gaaacgaat ggataaaga agctgagtc 
      acttgaacc gatcaatat caaagaggt tttggcatg 
      ttttactac aaatatcat cggttcctg tgcttttgg 
      actcgactg taaggctct ggaagatga aaattctcc 
      gtttcgtca gtttgggaa aaaattatt taagatatc 
      agttctcca ccgcttgaa acgcacctt tgctgcgac 
      tttccccga ctttgcaaa gtttttacg gatgtcaaa 
      cagtcggaa agaaatcgt caagtttat tatggacat 
      ggtagaagg tacgattga atatcgaga gaaaaacaa 
      ttactccag aaacgattt tatgcaact tttgatcga 
      catgaagaa caacaaacc aaagggcga tcacgatgg 
      caaaccctt gacccttga cgaagtcgc atctcaagc 
      tatagtttt ctttgctgc aggttttga aacttcgtc 
      aactctcat gactttcgc cttctacga acttgccaa 
      aaaccctca catccaaga caagttgag agaggaaat 
      taatttaat tctggcaga acataataa tgaaattag 
      ttatgacgc catccaaga aatgaaata cttaagcca 
      agtgattga cgagacttt aagactgca ccctccagc 
      agcacaaac tagtcgcaa gtgcattaa agattacaa 
      aattccgga ccaagatat tacaatcga gaaaggaac 
      aagtgttac agtttcaat tctggggat tcatcacga 
      cccggatta ttatccgga cccggaaaa attcgaccc 
      agaacgatt cactgaaga aaataagag tttaaggca 
      taattatgc gtttttgcc gtttggtga aggtcccag 
      aaactgcat tggaatgcg gtttggtct cttgcagtc 
      gaagatggg cttggtttc attaatcaa aaattataa 
      atttacagt gaatgaaaa gaccaaaga gccaattac 
      ttaccagcc agcgcatgt tgttttggc tgctcaggg 
      agaaatatg gctcaatgc ccaaaaagt ataatttta 
      tccttatac aatagccaa attgttcat taaaaagat 
      aagaaataa aaaacacaa gattcccat tcgtatctc 
      ttgagtaaa aaatgtgta gtgttcgtt taaatttgc 
      ggtcaaatg tttcaaagc taaaataat taatttaat 
      gtagatatt cagacttta cttgcgatc ccttgtgat 
      atacaaggt gtgttaaaa tgattctcg tcaaattat 
      ccttgaaat tggtttaca tgtaaaaca atttctacc 
      gctttgctc atttgaatt cgctgtcaa taaatgtta 
      aaactgtca gtagtgaaa ttcaattaa aaagcaaat 
      aacatgtta cactttgcg gaaaacact tttattacc 
      gaagcatat taataaaca tagtcatta atatgtcac 
      ttttgcaca gtcactccc caaatacaa aattcatat 
      ctgcatatt ctttgtttg aatattcga ccatattgc 
      aaaacttta aattagata tttgtttta tcaaaaaat 
      tgtataaag gttttcgtt cccattttg cctcataaa 
      ctcaccctg aaattcttg ggagacaat tgcctaaca 
      ccctgtgta cgaaattca caggcgacc agctgaaaa 
      tcattttaa cactacttg tataatgaa tcgtttgta 
      attcaagag aaataaaaa aattaacta tgtaaacta 
      aagtcagtt tttcttatt tctacattt gtcattttg 
      tacaaatgt atgaatgat tttaaaata aatgtttgg 
      tgtgccgag ttttaaact gtctttttg taaatcgac 
      atcatgttt atacctaca tttataata aacacagaa 
      ataaagtaa tttggtctt a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY