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Gene information
General information
TC010254 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450712.1: 140121..142632
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC010254
    iBeetle screen iB_04799

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010254 RA
      MFVSNSLLG DLIALFVTL GVLLTFYFK WTYQYWTRR 
NVPQIDPHI PFGNISESI KGREIVGVT FKKIYQQLK 
SQGCRHGGI YIFTQPSYF PIDLDYIRN ILTKDFQYF 
VDRPFYVNE KDPLQAHLL NLSGTRWRN MRSKLTPTF 
TSGKMKLMF HIMAESQSD LLKRMDKEA ESLEPINIK 
EVLACFTTN IIGSCAFGL DCKALEDEN SPFRQFGKK 
LFKISVLHR LKRTFAATF PDFAKFLRM SNSRKEIVK 
FIMDMVEGT IEYREKNNY SRNDFMQLL IDMKNNKPK 
GDHDGKPLT LDEVASQAI VFFAAGFET SSTLMTFAF 
YELAKNPHI QDKLREEIN LILAEHNNE ISYDAIQEM 
KYLSQVIDE TLRLHPPAA QTSRKCIKD YKIPDQDIT 
IEKGTSVTV SILGIHHDP DYYPDPEKF DPERFTEEN 
KSLRHNYAF LPFGEGPRN CIGMRFGLL QSKMGLVSL 
IKNYKFTVN EKTKEPITY QPAHVVLAA QGEIWLNAQ 
KV
    CDS sequence
    • >TC010254 RA
      atgtttgtt tccaactcg ttacttggc gatttgatc 
gctcttttt gtaactctt ggggtgctc ctaactttt 
tatttcaaa tggacttac cagtactgg accagacgt 
aacgtccct caaattgac cctcacata ccttttgga 
aacatctct gagtcaatc aagggccga gagattgtg 
ggagtgaca ttcaagaaa atctaccaa caactcaaa 
agtcaaggg tgcagacat ggtggcatt tacattttc 
acacaacca tcgtatttt cccattgac ttggattat 
attcgtaat attttgacg aaagatttt caatatttc 
gtcgatcgg cccttttat gtcaatgag aaagatcca 
ctccaagca catctcttg aatttgagt ggcactagg 
tggcgcaat atgaggtcc aagttgact ccaactttc 
acctcaggt aagatgaaa ctgatgttc catataatg 
gcagaaagt cagagcgat ttactgaaa cgaatggat 
aaagaagct gagtcactt gaaccgatc aatatcaaa 
gaggttttg gcatgtttt actacaaat atcatcggt 
tcctgtgct tttggactc gactgtaag gctctggaa 
gatgaaaat tctccgttt cgtcagttt gggaaaaaa 
ttatttaag atatcagtt ctccaccgc ttgaaacgc 
acctttgct gcgactttc cccgacttt gcaaagttt 
ttacggatg tcaaacagt cggaaagaa atcgtcaag 
tttattatg gacatggta gaaggtacg attgaatat 
cgagagaaa aacaattac tccagaaac gattttatg 
caacttttg atcgacatg aagaacaac aaaccaaag 
ggcgatcac gatggcaaa cccttgacc cttgacgaa 
gtcgcatct caagctata gttttcttt gctgcaggt 
tttgaaact tcgtcaact ctcatgact ttcgccttc 
tacgaactt gccaaaaac cctcacatc caagacaag 
ttgagagag gaaattaat ttaattctg gcagaacat 
aataatgaa attagttat gacgccatc caagaaatg 
aaatactta agccaagtg attgacgag actttaaga 
ctgcaccct ccagcagca caaactagt cgcaagtgc 
attaaagat tacaaaatt ccggaccaa gatattaca 
atcgagaaa ggaacaagt gttacagtt tcaattctg 
gggattcat cacgacccg gattattat ccggacccg 
gaaaaattc gacccagaa cgattcact gaagaaaat 
aagagttta aggcataat tatgcgttt ttgccgttt 
ggtgaaggt cccagaaac tgcattgga atgcggttt 
ggtctcttg cagtcgaag atgggcttg gtttcatta 
atcaaaaat tataaattt acagtgaat gaaaagacc 
aaagagcca attacttac cagccagcg catgttgtt 
ttggctgct cagggagaa atatggctc aatgcccaa 
aaagtataa
    mRNA sequence
    • >TC010254 RA
      tgtttacaa tattttgtc agcatgagt gcgtgcacc 
gagaaaata attctatat aaatatgtt ggctcgttt 
cttaatcta gtgtaatca agccatgtt tgtttccaa 
ctcgttact tggcgattt gatcgctct ttttgtaac 
tcttggggt gctcctaac tttttattt caaatggac 
ttaccagta ctggaccag acgtaacgt ccctcaaat 
tgaccctca cataccttt tggaaacat ctctgagtc 
aatcaaggg ccgagagat tgtgggagt gacattcaa 
gaaaatcta ccaacaact caaaagtca agggtgcag 
acatggtgg catttacat tttcacaca accatcgta 
ttttcccat tgacttgga ttatattcg taatatttt 
gacgaaaga ttttcaata tttcgtcga tcggccctt 
ttatgtcaa tgagaaaga tccactcca agcacatct 
cttgaattt gagtggcac taggtggcg caatatgag 
gtccaagtt gactccaac tttcacctc aggtaagat 
gaaactgat gttccatat aatggcaga aagtcagag 
cgatttact gaaacgaat ggataaaga agctgagtc 
acttgaacc gatcaatat caaagaggt tttggcatg 
ttttactac aaatatcat cggttcctg tgcttttgg 
actcgactg taaggctct ggaagatga aaattctcc 
gtttcgtca gtttgggaa aaaattatt taagatatc 
agttctcca ccgcttgaa acgcacctt tgctgcgac 
tttccccga ctttgcaaa gtttttacg gatgtcaaa 
cagtcggaa agaaatcgt caagtttat tatggacat 
ggtagaagg tacgattga atatcgaga gaaaaacaa 
ttactccag aaacgattt tatgcaact tttgatcga 
catgaagaa caacaaacc aaagggcga tcacgatgg 
caaaccctt gacccttga cgaagtcgc atctcaagc 
tatagtttt ctttgctgc aggttttga aacttcgtc 
aactctcat gactttcgc cttctacga acttgccaa 
aaaccctca catccaaga caagttgag agaggaaat 
taatttaat tctggcaga acataataa tgaaattag 
ttatgacgc catccaaga aatgaaata cttaagcca 
agtgattga cgagacttt aagactgca ccctccagc 
agcacaaac tagtcgcaa gtgcattaa agattacaa 
aattccgga ccaagatat tacaatcga gaaaggaac 
aagtgttac agtttcaat tctggggat tcatcacga 
cccggatta ttatccgga cccggaaaa attcgaccc 
agaacgatt cactgaaga aaataagag tttaaggca 
taattatgc gtttttgcc gtttggtga aggtcccag 
aaactgcat tggaatgcg gtttggtct cttgcagtc 
gaagatggg cttggtttc attaatcaa aaattataa 
atttacagt gaatgaaaa gaccaaaga gccaattac 
ttaccagcc agcgcatgt tgttttggc tgctcaggg 
agaaatatg gctcaatgc ccaaaaagt ataatttta 
tccttatac aatagccaa attgttcat taaaaagat 
aagaaataa aaaacacaa gattcccat tcgtatctc 
ttgagtaaa aaatgtgta gtgttcgtt taaatttgc 
ggtcaaatg tttcaaagc taaaataat taatttaat 
gtagatatt cagacttta cttgcgatc ccttgtgat 
atacaaggt gtgttaaaa tgattctcg tcaaattat 
ccttgaaat tggtttaca tgtaaaaca atttctacc 
gctttgctc atttgaatt cgctgtcaa taaatgtta 
aaactgtca gtagtgaaa ttcaattaa aaagcaaat 
aacatgtta cactttgcg gaaaacact tttattacc 
gaagcatat taataaaca tagtcatta atatgtcac 
ttttgcaca gtcactccc caaatacaa aattcatat 
ctgcatatt ctttgtttg aatattcga ccatattgc 
aaaacttta aattagata tttgtttta tcaaaaaat 
tgtataaag gttttcgtt cccattttg cctcataaa 
ctcaccctg aaattcttg ggagacaat tgcctaaca 
ccctgtgta cgaaattca caggcgacc agctgaaaa 
tcattttaa cactacttg tataatgaa tcgtttgta 
attcaagag aaataaaaa aattaacta tgtaaacta 
aagtcagtt tttcttatt tctacattt gtcattttg 
tacaaatgt atgaatgat tttaaaata aatgtttgg 
tgtgccgag ttttaaact gtctttttg taaatcgac 
atcatgttt atacctaca tttataata aacacagaa 
ataaagtaa tttggtctt a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY