GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04799 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
- >TC010254 RA
MFVSNSLLG DLIALFVTL GVLLTFYFK WTYQYWTRR NVPQIDPHI PFGNISESI KGREIVGVT FKKIYQQLK SQGCRHGGI YIFTQPSYF PIDLDYIRN ILTKDFQYF VDRPFYVNE KDPLQAHLL NLSGTRWRN MRSKLTPTF TSGKMKLMF HIMAESQSD LLKRMDKEA ESLEPINIK EVLACFTTN IIGSCAFGL DCKALEDEN SPFRQFGKK LFKISVLHR LKRTFAATF PDFAKFLRM SNSRKEIVK FIMDMVEGT IEYREKNNY SRNDFMQLL IDMKNNKPK GDHDGKPLT LDEVASQAI VFFAAGFET SSTLMTFAF YELAKNPHI QDKLREEIN LILAEHNNE ISYDAIQEM KYLSQVIDE TLRLHPPAA QTSRKCIKD YKIPDQDIT IEKGTSVTV SILGIHHDP DYYPDPEKF DPERFTEEN KSLRHNYAF LPFGEGPRN CIGMRFGLL QSKMGLVSL IKNYKFTVN EKTKEPITY QPAHVVLAA QGEIWLNAQ KV
- >TC010254 RA
atgtttgtt tccaactcg ttacttggc gatttgatc gctcttttt gtaactctt ggggtgctc ctaactttt tatttcaaa tggacttac cagtactgg accagacgt aacgtccct caaattgac cctcacata ccttttgga aacatctct gagtcaatc aagggccga gagattgtg ggagtgaca ttcaagaaa atctaccaa caactcaaa agtcaaggg tgcagacat ggtggcatt tacattttc acacaacca tcgtatttt cccattgac ttggattat attcgtaat attttgacg aaagatttt caatatttc gtcgatcgg cccttttat gtcaatgag aaagatcca ctccaagca catctcttg aatttgagt ggcactagg tggcgcaat atgaggtcc aagttgact ccaactttc acctcaggt aagatgaaa ctgatgttc catataatg gcagaaagt cagagcgat ttactgaaa cgaatggat aaagaagct gagtcactt gaaccgatc aatatcaaa gaggttttg gcatgtttt actacaaat atcatcggt tcctgtgct tttggactc gactgtaag gctctggaa gatgaaaat tctccgttt cgtcagttt gggaaaaaa ttatttaag atatcagtt ctccaccgc ttgaaacgc acctttgct gcgactttc cccgacttt gcaaagttt ttacggatg tcaaacagt cggaaagaa atcgtcaag tttattatg gacatggta gaaggtacg attgaatat cgagagaaa aacaattac tccagaaac gattttatg caacttttg atcgacatg aagaacaac aaaccaaag ggcgatcac gatggcaaa cccttgacc cttgacgaa gtcgcatct caagctata gttttcttt gctgcaggt tttgaaact tcgtcaact ctcatgact ttcgccttc tacgaactt gccaaaaac cctcacatc caagacaag ttgagagag gaaattaat ttaattctg gcagaacat aataatgaa attagttat gacgccatc caagaaatg aaatactta agccaagtg attgacgag actttaaga ctgcaccct ccagcagca caaactagt cgcaagtgc attaaagat tacaaaatt ccggaccaa gatattaca atcgagaaa ggaacaagt gttacagtt tcaattctg gggattcat cacgacccg gattattat ccggacccg gaaaaattc gacccagaa cgattcact gaagaaaat aagagttta aggcataat tatgcgttt ttgccgttt ggtgaaggt cccagaaac tgcattgga atgcggttt ggtctcttg cagtcgaag atgggcttg gtttcatta atcaaaaat tataaattt acagtgaat gaaaagacc aaagagcca attacttac cagccagcg catgttgtt ttggctgct cagggagaa atatggctc aatgcccaa aaagtataa
- >TC010254 RA
tgtttacaa tattttgtc agcatgagt gcgtgcacc gagaaaata attctatat aaatatgtt ggctcgttt cttaatcta gtgtaatca agccatgtt tgtttccaa ctcgttact tggcgattt gatcgctct ttttgtaac tcttggggt gctcctaac tttttattt caaatggac ttaccagta ctggaccag acgtaacgt ccctcaaat tgaccctca cataccttt tggaaacat ctctgagtc aatcaaggg ccgagagat tgtgggagt gacattcaa gaaaatcta ccaacaact caaaagtca agggtgcag acatggtgg catttacat tttcacaca accatcgta ttttcccat tgacttgga ttatattcg taatatttt gacgaaaga ttttcaata tttcgtcga tcggccctt ttatgtcaa tgagaaaga tccactcca agcacatct cttgaattt gagtggcac taggtggcg caatatgag gtccaagtt gactccaac tttcacctc aggtaagat gaaactgat gttccatat aatggcaga aagtcagag cgatttact gaaacgaat ggataaaga agctgagtc acttgaacc gatcaatat caaagaggt tttggcatg ttttactac aaatatcat cggttcctg tgcttttgg actcgactg taaggctct ggaagatga aaattctcc gtttcgtca gtttgggaa aaaattatt taagatatc agttctcca ccgcttgaa acgcacctt tgctgcgac tttccccga ctttgcaaa gtttttacg gatgtcaaa cagtcggaa agaaatcgt caagtttat tatggacat ggtagaagg tacgattga atatcgaga gaaaaacaa ttactccag aaacgattt tatgcaact tttgatcga catgaagaa caacaaacc aaagggcga tcacgatgg caaaccctt gacccttga cgaagtcgc atctcaagc tatagtttt ctttgctgc aggttttga aacttcgtc aactctcat gactttcgc cttctacga acttgccaa aaaccctca catccaaga caagttgag agaggaaat taatttaat tctggcaga acataataa tgaaattag ttatgacgc catccaaga aatgaaata cttaagcca agtgattga cgagacttt aagactgca ccctccagc agcacaaac tagtcgcaa gtgcattaa agattacaa aattccgga ccaagatat tacaatcga gaaaggaac aagtgttac agtttcaat tctggggat tcatcacga cccggatta ttatccgga cccggaaaa attcgaccc agaacgatt cactgaaga aaataagag tttaaggca taattatgc gtttttgcc gtttggtga aggtcccag aaactgcat tggaatgcg gtttggtct cttgcagtc gaagatggg cttggtttc attaatcaa aaattataa atttacagt gaatgaaaa gaccaaaga gccaattac ttaccagcc agcgcatgt tgttttggc tgctcaggg agaaatatg gctcaatgc ccaaaaagt ataatttta tccttatac aatagccaa attgttcat taaaaagat aagaaataa aaaacacaa gattcccat tcgtatctc ttgagtaaa aaatgtgta gtgttcgtt taaatttgc ggtcaaatg tttcaaagc taaaataat taatttaat gtagatatt cagacttta cttgcgatc ccttgtgat atacaaggt gtgttaaaa tgattctcg tcaaattat ccttgaaat tggtttaca tgtaaaaca atttctacc gctttgctc atttgaatt cgctgtcaa taaatgtta aaactgtca gtagtgaaa ttcaattaa aaagcaaat aacatgtta cactttgcg gaaaacact tttattacc gaagcatat taataaaca tagtcatta atatgtcac ttttgcaca gtcactccc caaatacaa aattcatat ctgcatatt ctttgtttg aatattcga ccatattgc aaaacttta aattagata tttgtttta tcaaaaaat tgtataaag gttttcgtt cccattttg cctcataaa ctcaccctg aaattcttg ggagacaat tgcctaaca ccctgtgta cgaaattca caggcgacc agctgaaaa tcattttaa cactacttg tataatgaa tcgtttgta attcaagag aaataaaaa aattaacta tgtaaacta aagtcagtt tttcttatt tctacattt gtcattttg tacaaatgt atgaatgat tttaaaata aatgtttgg tgtgccgag ttttaaact gtctttttg taaatcgac atcatgttt atacctaca tttataata aacacagaa ataaagtaa tttggtctt a
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
