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Gene information
General information
TC010251 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450712.1: 76704..78317
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC010251
    iBeetle screen iB_04797

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection

    Adult morphology
    abdominal odoriferous glands size decreased - Number of animals showing the phenotype: 7 (41 dpi)
    Days post injection


    abdominal odoriferous glands colour lighter - Number of animals showing the phenotype: 7 (41 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010251 RA
      MNIPSPYFE ILGTVLALL VAIIFYYHH AFQFWARKN 
IPFIPPRFP LGNTNVVLP RGLTIGLLS KTFYDYFKQ 
RGHKIGGVF LVTEPNLMI LDPDYIKNI LLKDFHHFV 
DRGFYFNEK TDPISANLF TIDGDQWRN LRTKLTPTF 
TSSKMKIMF QTVKNCCNN MLEAIASNT DLDKDLKEF 
LGRYTVDVV GSCAFGIEC NSFKHPDAE FRKMGLRAF 
HFSFLRSIR AFFIAYLPK LSRQMGLPS NNKDIREFF 
YKVVMETIQ KREETQIKR NDFLQILMD LRKTEILSL 
DEVAAQVFL FFTAGFETS SSTMAMCLY EIAKEPEYQ 
EKLRQEICE ITNGEITYE NLFEMKYLD QVFSETLRK 
YPPGQTLNR RCVKDYTLP GTSTIIEKG TPILISAIG 
VHRDPEYYP DPEKFDPER FSEENKKLR HPFVYLPFG 
DGPRNCIGM RFGTMQSKL GIASVVKNF KVSVSPITK 
RVELDPNTF LLNTIDKIY FRVEKIVK
    CDS sequence
    • >TC010251 RA
      atgaacatc ccaagcccc tatttcgag attttaggc 
actgtttta gcccttcta gttgcaata attttctat 
taccaccac gccttccag ttctgggcc agaaaaaac 
atccctttt atccctcca cgctttcca ctcggcaac 
accaatgta gttctacca cgaggatta acaatcgga 
ttacttagc aagactttt tacgactat ttcaagcaa 
cgtggccat aaaatcggt ggtgttttc ctagtgact 
gaaccaaac ctcatgatt ttggacccc gactacatc 
aagaatatc cttttgaaa gactttcac cattttgta 
gatcgaggg ttttacttc aatgagaaa actgaccca 
atcagcgcc aatttgttc acaattgat ggcgatcag 
tggagaaat ctccgaact aaactcacc ccaactttc 
acctcaagt aaaatgaaa atcatgttc caaaccgtg 
aaaaactgc tgcaacaac atgcttgaa gctattgca 
agtaacact gacctggac aaagatttg aaggagttt 
ctgggacgt tacactgtt gatgttgtt gggtcttgt 
gcttttggt attgagtgt aatagtttc aagcaccca 
gatgccgaa tttcgaaaa atgggtctt agggcgttt 
cactttagt tttttgaga tcaattcgg gcttttttt 
attgcttat ttgcccaaa ttgtcgcgc caaatgggc 
cttccaagc aacaataaa gacattagg gagtttttc 
tacaaagtt gtgatggag acaatacag aaacgggag 
gaaactcaa atcaaacgt aatgatttt ttacaaatc 
ttgatggat ttgagaaag acggaaatt ttgagtttg 
gacgaagta gcagctcag gtgtttctc tttttcaca 
gctgggttt gagacgagt tctagcact atggcaatg 
tgtctgtat gagattgcc aaagaacca gaatatcaa 
gagaagttg agacaagag atttgcgaa ataactaac 
ggcgaaatc acctatgaa aatctattt gaaatgaaa 
tatctagac caggttttt tcagaaacc cttcgaaaa 
tatccccca ggtcaaact ttgaatcgc cgctgtgtt 
aaagactac acccttcca ggaactagc acaatcatt 
gaaaaaggg actcccatc cttatttct gcaatagga 
gttcacagg gatcctgag tattatcca gatcctgag 
aaatttgat cctgagagg tttagtgaa gaaaataaa 
aaattgaga catccgttc gtttatttg ccatttggg 
gatggacca agaaattgt attggaatg cgatttgga 
actatgcag tctaagtta gggattgca tcagttgtg 
aaaaatttt aaagtgtct gttagtccc ataacaaag 
cgggttgaa ctggatccg aacactttt ctcctgaat 
acaattgat aaaatttat tttagagta gaaaaaatt 
gtgaaatag
    mRNA sequence
    • >TC010251 RA
      tcgtctacg aagtgacaa tgaacatcc caagcccct 
atttcgaga ttttaggca ctgttttag cccttctag 
ttgcaataa ttttctatt accaccacg ccttccagt 
tctgggcca gaaaaaaca tccctttta tccctccac 
gctttccac tcggcaaca ccaatgtag ttctaccac 
gaggattaa caatcggat tacttagca agacttttt 
acgactatt tcaagcaac gtggccata aaatcggtg 
gtgttttcc tagtgactg aaccaaacc tcatgattt 
tggaccccg actacatca agaatatcc ttttgaaag 
actttcacc attttgtag atcgagggt tttacttca 
atgagaaaa ctgacccaa tcagcgcca atttgttca 
caattgatg gcgatcagt ggagaaatc tccgaacta 
aactcaccc caactttca cctcaagta aaatgaaaa 
tcatgttcc aaaccgtga aaaactgct gcaacaaca 
tgcttgaag ctattgcaa gtaacactg acctggaca 
aagatttga aggagtttc tgggacgtt acactgttg 
atgttgttg ggtcttgtg cttttggta ttgagtgta 
atagtttca agcacccag atgccgaat ttcgaaaaa 
tgggtctta gggcgtttc actttagtt ttttgagat 
caattcggg cttttttta ttgcttatt tgcccaaat 
tgtcgcgcc aaatgggcc ttccaagca acaataaag 
acattaggg agtttttct acaaagttg tgatggaga 
caatacaga aacgggagg aaactcaaa tcaaacgta 
atgattttt tacaaatct tgatggatt tgagaaaga 
cggaaattt tgagtttgg acgaagtag cagctcagg 
tgtttctct ttttcacag ctgggtttg agacgagtt 
ctagcacta tggcaatgt gtctgtatg agattgcca 
aagaaccag aatatcaag agaagttga gacaagaga 
tttgcgaaa taactaacg gcgaaatca cctatgaaa 
atctatttg aaatgaaat atctagacc aggtttttt 
cagaaaccc ttcgaaaat atcccccag gtcaaactt 
tgaatcgcc gctgtgtta aagactaca cccttccag 
gaactagca caatcattg aaaaaggga ctcccatcc 
ttatttctg caataggag ttcacaggg atcctgagt 
attatccag atcctgaga aatttgatc ctgagaggt 
ttagtgaag aaaataaaa aattgagac atccgttcg 
tttatttgc catttgggg atggaccaa gaaattgta 
ttggaatgc gatttggaa ctatgcagt ctaagttag 
ggattgcat cagttgtga aaaatttta aagtgtctg 
ttagtccca taacaaagc gggttgaac tggatccga 
acacttttc tcctgaata caattgata aaatttatt 
ttagagtag aaaaaattg tgaaatagg caaaataat 
tcgttgctt tgatgtaat gaaatgaaa ataaatcat 
attttaaca a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY