GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04797 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
abdominal odoriferous glands colour lighter - Number of animals showing the phenotype: 7 (41 dpi)
- >TC010251 RA
MNIPSPYFE ILGTVLALL VAIIFYYHH AFQFWARKN IPFIPPRFP LGNTNVVLP RGLTIGLLS KTFYDYFKQ RGHKIGGVF LVTEPNLMI LDPDYIKNI LLKDFHHFV DRGFYFNEK TDPISANLF TIDGDQWRN LRTKLTPTF TSSKMKIMF QTVKNCCNN MLEAIASNT DLDKDLKEF LGRYTVDVV GSCAFGIEC NSFKHPDAE FRKMGLRAF HFSFLRSIR AFFIAYLPK LSRQMGLPS NNKDIREFF YKVVMETIQ KREETQIKR NDFLQILMD LRKTEILSL DEVAAQVFL FFTAGFETS SSTMAMCLY EIAKEPEYQ EKLRQEICE ITNGEITYE NLFEMKYLD QVFSETLRK YPPGQTLNR RCVKDYTLP GTSTIIEKG TPILISAIG VHRDPEYYP DPEKFDPER FSEENKKLR HPFVYLPFG DGPRNCIGM RFGTMQSKL GIASVVKNF KVSVSPITK RVELDPNTF LLNTIDKIY FRVEKIVK
- >TC010251 RA
atgaacatc ccaagcccc tatttcgag attttaggc actgtttta gcccttcta gttgcaata attttctat taccaccac gccttccag ttctgggcc agaaaaaac atccctttt atccctcca cgctttcca ctcggcaac accaatgta gttctacca cgaggatta acaatcgga ttacttagc aagactttt tacgactat ttcaagcaa cgtggccat aaaatcggt ggtgttttc ctagtgact gaaccaaac ctcatgatt ttggacccc gactacatc aagaatatc cttttgaaa gactttcac cattttgta gatcgaggg ttttacttc aatgagaaa actgaccca atcagcgcc aatttgttc acaattgat ggcgatcag tggagaaat ctccgaact aaactcacc ccaactttc acctcaagt aaaatgaaa atcatgttc caaaccgtg aaaaactgc tgcaacaac atgcttgaa gctattgca agtaacact gacctggac aaagatttg aaggagttt ctgggacgt tacactgtt gatgttgtt gggtcttgt gcttttggt attgagtgt aatagtttc aagcaccca gatgccgaa tttcgaaaa atgggtctt agggcgttt cactttagt tttttgaga tcaattcgg gcttttttt attgcttat ttgcccaaa ttgtcgcgc caaatgggc cttccaagc aacaataaa gacattagg gagtttttc tacaaagtt gtgatggag acaatacag aaacgggag gaaactcaa atcaaacgt aatgatttt ttacaaatc ttgatggat ttgagaaag acggaaatt ttgagtttg gacgaagta gcagctcag gtgtttctc tttttcaca gctgggttt gagacgagt tctagcact atggcaatg tgtctgtat gagattgcc aaagaacca gaatatcaa gagaagttg agacaagag atttgcgaa ataactaac ggcgaaatc acctatgaa aatctattt gaaatgaaa tatctagac caggttttt tcagaaacc cttcgaaaa tatccccca ggtcaaact ttgaatcgc cgctgtgtt aaagactac acccttcca ggaactagc acaatcatt gaaaaaggg actcccatc cttatttct gcaatagga gttcacagg gatcctgag tattatcca gatcctgag aaatttgat cctgagagg tttagtgaa gaaaataaa aaattgaga catccgttc gtttatttg ccatttggg gatggacca agaaattgt attggaatg cgatttgga actatgcag tctaagtta gggattgca tcagttgtg aaaaatttt aaagtgtct gttagtccc ataacaaag cgggttgaa ctggatccg aacactttt ctcctgaat acaattgat aaaatttat tttagagta gaaaaaatt gtgaaatag
- >TC010251 RA
tcgtctacg aagtgacaa tgaacatcc caagcccct atttcgaga ttttaggca ctgttttag cccttctag ttgcaataa ttttctatt accaccacg ccttccagt tctgggcca gaaaaaaca tccctttta tccctccac gctttccac tcggcaaca ccaatgtag ttctaccac gaggattaa caatcggat tacttagca agacttttt acgactatt tcaagcaac gtggccata aaatcggtg gtgttttcc tagtgactg aaccaaacc tcatgattt tggaccccg actacatca agaatatcc ttttgaaag actttcacc attttgtag atcgagggt tttacttca atgagaaaa ctgacccaa tcagcgcca atttgttca caattgatg gcgatcagt ggagaaatc tccgaacta aactcaccc caactttca cctcaagta aaatgaaaa tcatgttcc aaaccgtga aaaactgct gcaacaaca tgcttgaag ctattgcaa gtaacactg acctggaca aagatttga aggagtttc tgggacgtt acactgttg atgttgttg ggtcttgtg cttttggta ttgagtgta atagtttca agcacccag atgccgaat ttcgaaaaa tgggtctta gggcgtttc actttagtt ttttgagat caattcggg cttttttta ttgcttatt tgcccaaat tgtcgcgcc aaatgggcc ttccaagca acaataaag acattaggg agtttttct acaaagttg tgatggaga caatacaga aacgggagg aaactcaaa tcaaacgta atgattttt tacaaatct tgatggatt tgagaaaga cggaaattt tgagtttgg acgaagtag cagctcagg tgtttctct ttttcacag ctgggtttg agacgagtt ctagcacta tggcaatgt gtctgtatg agattgcca aagaaccag aatatcaag agaagttga gacaagaga tttgcgaaa taactaacg gcgaaatca cctatgaaa atctatttg aaatgaaat atctagacc aggtttttt cagaaaccc ttcgaaaat atcccccag gtcaaactt tgaatcgcc gctgtgtta aagactaca cccttccag gaactagca caatcattg aaaaaggga ctcccatcc ttatttctg caataggag ttcacaggg atcctgagt attatccag atcctgaga aatttgatc ctgagaggt ttagtgaag aaaataaaa aattgagac atccgttcg tttatttgc catttgggg atggaccaa gaaattgta ttggaatgc gatttggaa ctatgcagt ctaagttag ggattgcat cagttgtga aaaatttta aagtgtctg ttagtccca taacaaagc gggttgaac tggatccga acacttttc tcctgaata caattgata aaatttatt ttagagtag aaaaaattg tgaaatagg caaaataat tcgttgctt tgatgtaat gaaatgaaa ataaatcat attttaaca a
