Status
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Gene information
General information
TC010251 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450712.1: 76704..78317
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010251
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04797

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands size decreased - Number of animals showing the phenotype: 7 (41 dpi)
    Days post injection



    abdominal odoriferous glands colour lighter - Number of animals showing the phenotype: 7 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010251 RA
      MNIPSPYFE ILGTVLALL VAIIFYYHH AFQFWARKN 
      IPFIPPRFP LGNTNVVLP RGLTIGLLS KTFYDYFKQ 
      RGHKIGGVF LVTEPNLMI LDPDYIKNI LLKDFHHFV 
      DRGFYFNEK TDPISANLF TIDGDQWRN LRTKLTPTF 
      TSSKMKIMF QTVKNCCNN MLEAIASNT DLDKDLKEF 
      LGRYTVDVV GSCAFGIEC NSFKHPDAE FRKMGLRAF 
      HFSFLRSIR AFFIAYLPK LSRQMGLPS NNKDIREFF 
      YKVVMETIQ KREETQIKR NDFLQILMD LRKTEILSL 
      DEVAAQVFL FFTAGFETS SSTMAMCLY EIAKEPEYQ 
      EKLRQEICE ITNGEITYE NLFEMKYLD QVFSETLRK 
      YPPGQTLNR RCVKDYTLP GTSTIIEKG TPILISAIG 
      VHRDPEYYP DPEKFDPER FSEENKKLR HPFVYLPFG 
      DGPRNCIGM RFGTMQSKL GIASVVKNF KVSVSPITK 
      RVELDPNTF LLNTIDKIY FRVEKIVK
    CDS sequence
    • >TC010251 RA
      atgaacatc ccaagcccc tatttcgag attttaggc 
      actgtttta gcccttcta gttgcaata attttctat 
      taccaccac gccttccag ttctgggcc agaaaaaac 
      atccctttt atccctcca cgctttcca ctcggcaac 
      accaatgta gttctacca cgaggatta acaatcgga 
      ttacttagc aagactttt tacgactat ttcaagcaa 
      cgtggccat aaaatcggt ggtgttttc ctagtgact 
      gaaccaaac ctcatgatt ttggacccc gactacatc 
      aagaatatc cttttgaaa gactttcac cattttgta 
      gatcgaggg ttttacttc aatgagaaa actgaccca 
      atcagcgcc aatttgttc acaattgat ggcgatcag 
      tggagaaat ctccgaact aaactcacc ccaactttc 
      acctcaagt aaaatgaaa atcatgttc caaaccgtg 
      aaaaactgc tgcaacaac atgcttgaa gctattgca 
      agtaacact gacctggac aaagatttg aaggagttt 
      ctgggacgt tacactgtt gatgttgtt gggtcttgt 
      gcttttggt attgagtgt aatagtttc aagcaccca 
      gatgccgaa tttcgaaaa atgggtctt agggcgttt 
      cactttagt tttttgaga tcaattcgg gcttttttt 
      attgcttat ttgcccaaa ttgtcgcgc caaatgggc 
      cttccaagc aacaataaa gacattagg gagtttttc 
      tacaaagtt gtgatggag acaatacag aaacgggag 
      gaaactcaa atcaaacgt aatgatttt ttacaaatc 
      ttgatggat ttgagaaag acggaaatt ttgagtttg 
      gacgaagta gcagctcag gtgtttctc tttttcaca 
      gctgggttt gagacgagt tctagcact atggcaatg 
      tgtctgtat gagattgcc aaagaacca gaatatcaa 
      gagaagttg agacaagag atttgcgaa ataactaac 
      ggcgaaatc acctatgaa aatctattt gaaatgaaa 
      tatctagac caggttttt tcagaaacc cttcgaaaa 
      tatccccca ggtcaaact ttgaatcgc cgctgtgtt 
      aaagactac acccttcca ggaactagc acaatcatt 
      gaaaaaggg actcccatc cttatttct gcaatagga 
      gttcacagg gatcctgag tattatcca gatcctgag 
      aaatttgat cctgagagg tttagtgaa gaaaataaa 
      aaattgaga catccgttc gtttatttg ccatttggg 
      gatggacca agaaattgt attggaatg cgatttgga 
      actatgcag tctaagtta gggattgca tcagttgtg 
      aaaaatttt aaagtgtct gttagtccc ataacaaag 
      cgggttgaa ctggatccg aacactttt ctcctgaat 
      acaattgat aaaatttat tttagagta gaaaaaatt 
      gtgaaatag
    mRNA sequence
    • >TC010251 RA
      tcgtctacg aagtgacaa tgaacatcc caagcccct 
      atttcgaga ttttaggca ctgttttag cccttctag 
      ttgcaataa ttttctatt accaccacg ccttccagt 
      tctgggcca gaaaaaaca tccctttta tccctccac 
      gctttccac tcggcaaca ccaatgtag ttctaccac 
      gaggattaa caatcggat tacttagca agacttttt 
      acgactatt tcaagcaac gtggccata aaatcggtg 
      gtgttttcc tagtgactg aaccaaacc tcatgattt 
      tggaccccg actacatca agaatatcc ttttgaaag 
      actttcacc attttgtag atcgagggt tttacttca 
      atgagaaaa ctgacccaa tcagcgcca atttgttca 
      caattgatg gcgatcagt ggagaaatc tccgaacta 
      aactcaccc caactttca cctcaagta aaatgaaaa 
      tcatgttcc aaaccgtga aaaactgct gcaacaaca 
      tgcttgaag ctattgcaa gtaacactg acctggaca 
      aagatttga aggagtttc tgggacgtt acactgttg 
      atgttgttg ggtcttgtg cttttggta ttgagtgta 
      atagtttca agcacccag atgccgaat ttcgaaaaa 
      tgggtctta gggcgtttc actttagtt ttttgagat 
      caattcggg cttttttta ttgcttatt tgcccaaat 
      tgtcgcgcc aaatgggcc ttccaagca acaataaag 
      acattaggg agtttttct acaaagttg tgatggaga 
      caatacaga aacgggagg aaactcaaa tcaaacgta 
      atgattttt tacaaatct tgatggatt tgagaaaga 
      cggaaattt tgagtttgg acgaagtag cagctcagg 
      tgtttctct ttttcacag ctgggtttg agacgagtt 
      ctagcacta tggcaatgt gtctgtatg agattgcca 
      aagaaccag aatatcaag agaagttga gacaagaga 
      tttgcgaaa taactaacg gcgaaatca cctatgaaa 
      atctatttg aaatgaaat atctagacc aggtttttt 
      cagaaaccc ttcgaaaat atcccccag gtcaaactt 
      tgaatcgcc gctgtgtta aagactaca cccttccag 
      gaactagca caatcattg aaaaaggga ctcccatcc 
      ttatttctg caataggag ttcacaggg atcctgagt 
      attatccag atcctgaga aatttgatc ctgagaggt 
      ttagtgaag aaaataaaa aattgagac atccgttcg 
      tttatttgc catttgggg atggaccaa gaaattgta 
      ttggaatgc gatttggaa ctatgcagt ctaagttag 
      ggattgcat cagttgtga aaaatttta aagtgtctg 
      ttagtccca taacaaagc gggttgaac tggatccga 
      acacttttc tcctgaata caattgata aaatttatt 
      ttagagtag aaaaaattg tgaaatagg caaaataat 
      tcgttgctt tgatgtaat gaaatgaaa ataaatcat 
      attttaaca a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY