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Gene information
General information
TC010240 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450712.1: 104798..106289
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010240
    iBeetle screen iB_04795

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010240 RA
      MVLSMEKWV GKVAIVTGA SSGIGAAIA EKLVEQGLT 
      VVGVARRVA LIETQAQKL SNKKGKLHA VKADLTVET 
      DVLDAFKWT LENLGPVHI LVNNAGILK EELLTRGQS 
      EDWKRAFEV NVLALCIAT REAVKIMNA HNIDGHIIH 
      INSILGHHV AIEPKLNVY PATKHAVTA LTETLRQEL 
      NSLGSKIKV TSISPGLVV SELTLLSKN ISEERRKNF 
      EGRPILQAE DIADGVVYA LSTPPHVQV HELTIKPVG 
      EPI
    CDS sequence
    • >TC010240 RA
      atggttctc tcgatggaa aaatgggtt gggaaagtt 
      gcaatagta acaggtgct agttccgga attggggct 
      gctattgca gaaaaactt gttgaacaa ggcttaacg 
      gttgttgga gtcgcccgt cgagtcgca cttatcgaa 
      acccaagcc caaaaacta tcaaacaaa aaaggaaaa 
      ttacacgcc gtcaaagcc gatttaact gtggaaact 
      gatgttttg gacgcattt aaatggact ttggaaaat 
      ttaggcccg gtacacatt cttgtaaac aatgccggc 
      attttgaaa gaagaatta ctaacaagg ggccaatct 
      gaagactgg aaaagagca tttgaagtc aatgtttta 
      gccctgtgt attgcgaca agagaggca gttaaaata 
      atgaatgct cacaatatt gatggtcat attatccat 
      attaatagc attctaggc catcacgtg gcaattgag 
      ccaaaatta aacgtttac ccggcgact aaacatgcc 
      gtcacagct ttgacggag actttaaga caagaattg 
      aattctctg gggagcaaa ataaaagtg acgagcata 
      agtcctgga cttgtagta agtgaattg actcttttg 
      agtaaaaac atttcagaa gaaaggagg aaaaatttt 
      gagggaaga cctatttta caagcggaa gatattgca 
      gatggggtt gtttatgcc ttatcgact cctcctcac 
      gtccaggtg cacgagcta acaatcaaa ccggtagga 
      gaacccatt taa
    mRNA sequence
    • >TC010240 RA
      acattgacc ttacaccca cttatctgc tagcagcgt 
      taaacatgg ttctctcga tggaaaaat gggttggga 
      aagttgcaa tagtaacag gtgctagtt ccggaattg 
      gggctgcta ttgcagaaa aacttgttg aacaaggct 
      taacggttg ttggagtcg cccgtcgag tcgcactta 
      tcgaaaccc aagcccaaa aactatcaa acaaaaaag 
      gaaaattac acgccgtca aagccgatt taactgtgg 
      aaactgatg ttttggacg catttaaat ggactttgg 
      aaaatttag gcccggtac acattcttg taaacaatg 
      ccggcattt tgaaagaag aattactaa caaggggcc 
      aatctgaag actggaaaa gagcatttg aagtcaatg 
      ttttagccc tgtgtattg cgacaagag aggcagtta 
      aaataatga atgctcaca atattgatg gtcatatta 
      tccatatta atagcattc taggccatc acgtggcaa 
      ttgagccaa aattaaacg tttacccgg cgactaaac 
      atgccgtca cagctttga cggagactt taagacaag 
      aattgaatt ctctgggga gcaaaataa aagtgacga 
      gcataagtc ctggacttg tagtaagtg aattgactc 
      ttttgagta aaaacattt cagaagaaa ggaggaaaa 
      attttgagg gaagaccta ttttacaag cggaagata 
      ttgcagatg gggttgttt atgccttat cgactcctc 
      ctcacgtcc aggtgcacg agctaacaa tcaaaccgg 
      taggagaac ccatttaat cagttttaa agtcaaatg 
      aacgatttt gtgatttta gtacaagtt atttgaaat 
      aaaaatgct tacttttaa actttaaaa agtcaatca 
      atctatttg tttttgaca aaaatgcta atttttaaa 
      cccttttaa atattatct ggtatctta tcgcaaaac 
      agtttgtta cttatactg tgtcactct ctagaccac 
      tatcaaata aaaatgttt tttgactcg attagtctt 
      aacgtgtta agtgtattc attgctctg acagccgtt 
      atctttgct tactacaaa tggacttat cactattgg 
      gagcgccaa aaattacca tttttgacc ccgacaatc 
      ccttatggt aacatattg agtccctta agcaagacc 
      gaaaatttt gccatcact tgtaaacac ctttataac 
      aaaattaag caaagagag tggaaacat ggagggatt 
      tatgcaatt ctagcccct atttacttt gtcgtgaac 
      ttggattat gtgaaaaat ataatgacg aaagattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY