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Gene information
General information
TC010239 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450712.1: 109173..111929
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010239
    iBeetle screen iB_04794

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010239 RA
      MFTFVGVAI ALLATLFAY FKWTYKYWE RRNLQYLEP 
      KIPYGNGTG PFKRTENAG FRFKRQYEE LKSRGWKHG 
      GLYSILNPI YLVADLDYI KNIMTKDFH YFTDRGIYY 
      NEKNDPLSA HLFALGGPK WRHLRTKLS PTFTSGKMK 
      QMFQTLVDC VPQLLQQID KNVPVDIKE VLGCFTTDI 
      IGSCAFGLE CKTFEDENS PFREYGRKF FQVSNLKIA 
      KFLFSGNFP NVARTLGVT ITSKDVSDF FISVVEDTV 
      KYREKHNLV RKDFMQLLI DLKNTEKEQ MLTIEELAA 
      QCFVFFIAG FETSSTTMT FALFELAKR PDLQQQVRD 
      EIETVLAKH GNITYDAIQ DLKFMDQVI DETLRMYPP 
      VPVLTRKCV KDYKIPDQD VIIQKGTRV FIPVLGIHY 
      DSDLYPNPS QFDPDRFSE EKKKSRHGY AHLPFGEGP 
      RICIGMRFG LMQTKVGLT ALLKNYKFS VNSKTQTPL 
      KMKPNSFIL AAEGDVWLN AQKV
    CDS sequence
    • >TC010239 RA
      atgtttact tttgtgggc gttgcaatc gctcttttg 
      gctaccctt ttcgcctat ttcaaatgg acctacaaa 
      tactgggaa agacgcaac cttcaatac cttgaacct 
      aaaattccg tacggaaac ggaaccggt ccttttaaa 
      cgaacagaa aatgccggt tttcggttc aaacgccaa 
      tacgaggaa ctgaaatca agggggtgg aaacacggg 
      ggtttgtac tcaatcctc aaccccatt tacctagtc 
      gccgattta gactacatc aaaaatatt atgaccaaa 
      gacttccac tactttaca gaccgtggg atttactat 
      aacgaaaag aacgatcct cttagtgcc catttgttt 
      gctcttgga ggccccaag tggcggcat ttgagaacc 
      aaactaagc ccaactttc acatccggc aaaatgaaa 
      caaatgttt cagactttg gtcgattgt gttccacag 
      ttgttgcaa cagattgat aaaaatgta ccagttgat 
      attaaagaa gtgttaggg tgttttacc actgatata 
      atcgggtcg tgtgctttt ggactggaa tgcaagact 
      ttcgaagat gaaaactct ccgttcaga gagtatggg 
      cgaaaattt ttccaagtg tccaactta aaaattgcg 
      aaatttttg ttttcgggt aattttccc aatgtagca 
      cgaacgcta ggagtgacg atcacgtca aaggacgtt 
      tcagatttc tttatctca gtggtggaa gacacagta 
      aaatatcgc gaaaagcac aatttggtc cgcaaagac 
      ttcatgcaa ctgctcatc gatttgaaa aatactgag 
      aaagaacaa atgttaact attgaagaa ttggcagcc 
      cagtgcttc gtttttttc attgctggg tttgaaaca 
      tcttcaaca actatgact tttgcttta ttcgaactg 
      gccaaacga cccgatttg cagcaacaa gttcgtgac 
      gaaattgaa acggttttg gccaaacat ggaaatatc 
      acttacgat gccattcaa gacttgaag tttatggac 
      caagtgata gacgagaca ttgcggatg taccctcca 
      gttcccgta cttacacga aagtgtgtt aaggattac 
      aaaatccca gaccaggat gtgatcatc caaaaagga 
      accagagtc tttatcccg gttcttggc atccactat 
      gattctgat ttgtaccca aatccgagt caatttgac 
      ccagatcgg ttcagtgaa gagaagaaa aaatcaagg 
      catgggtat gcccacttg ccgtttggt gaaggaccc 
      agaatttgc atcggtatg cggttcggg ttaatgcaa 
      accaaggtt ggcctcact gctttatta aaaaattac 
      aagttttcg gtaaatagt aagacccaa acacccttg 
      aaaatgaaa cccaattcg tttattttg gccgcagag 
      ggagacgtg tggttgaat gcgcaaaaa gtttag
    mRNA sequence
    • >TC010239 RA
      ctattacaa actagaaga aatgtttac ttttgtggg 
      cgttgcaat cgctctttt ggctaccct tttcgccta 
      tttcaaatg gacctacaa atactggga aagacgcaa 
      ccttcaata ccttgaacc taaaattcc gtacggaaa 
      cggaaccgg tccttttaa acgaacaga aaatgccgg 
      ttttcggtt caaacgcca atacgagga actgaaatc 
      aagggggtg gaaacacgg gggtttgta ctcaatcct 
      caaccccat ttacctagt cgccgattt agactacat 
      caaaaatat tatgaccaa agacttcca ctactttac 
      agaccgtgg gatttacta taacgaaaa gaacgatcc 
      tcttagtgc ccatttgtt tgctcttgg aggccccaa 
      gtggcggca tttgagaac caaactaag cccaacttt 
      cacatccgg caaaatgaa acaaatgtt tcagacttt 
      ggtcgattg tgttccaca gttgttgca acagattga 
      taaaaatgt accagttga tattaaaga agtgttagg 
      gtgttttac cactgatat aatcgggtc gtgtgcttt 
      tggactgga atgcaagac tttcgaaga tgaaaactc 
      tccgttcag agagtatgg gcgaaaatt tttccaagt 
      gtccaactt aaaaattgc gaaattttt gttttcggg 
      taattttcc caatgtagc acgaacgct aggagtgac 
      gatcacgtc aaaggacgt ttcagattt ctttatctc 
      agtggtgga agacacagt aaaatatcg cgaaaagca 
      caatttggt ccgcaaaga cttcatgca actgctcat 
      cgatttgaa aaatactga gaaagaaca aatgttaac 
      tattgaaga attggcagc ccagtgctt cgttttttt 
      cattgctgg gtttgaaac atcttcaac aactatgac 
      ttttgcttt attcgaact ggccaaacg acccgattt 
      gcagcaaca agttcgtga cgaaattga aacggtttt 
      ggccaaaca tggaaatat cacttacga tgccattca 
      agacttgaa gtttatgga ccaagtgat agacgagac 
      attgcggat gtaccctcc agttcccgt acttacacg 
      aaagtgtgt taaggatta caaaatccc agaccagga 
      tgtgatcat ccaaaaagg aaccagagt ctttatccc 
      ggttcttgg catccacta tgattctga tttgtaccc 
      aaatccgag tcaatttga cccagatcg gttcagtga 
      agagaagaa aaaatcaag gcatgggta tgcccactt 
      gccgtttgg tgaaggacc cagaatttg catcggtat 
      gcggttcgg gttaatgca aaccaaggt tggcctcac 
      tgctttatt aaaaaatta caagttttc ggtaaatag 
      taagaccca aacaccctt gaaaatgaa acccaattc 
      gtttatttt ggccgcaga gggagacgt gtggttgaa 
      tgcgcaaaa agtttagtt taagttgca gatgtagga 
      agaatagac gatgttact catgcgttg tttcaatga 
      agttatcga attgacttc gaaccccaa atatttcga 
      tattttcaa ataccccaa atacgtttt taaaacact 
      tatgtataa aatataagt taggaattt tttaagaac 
      aaggagcaa gaaaatata aagtgtaat taagtaggt 
      actcatatc tcgaacagc taaacttat agcttggaa 
      tagcgactt gcaagaaaa taggaaaaa aaattaatc 
      cggagataa aaaatttaa atattatct aaaaaataa 
      tgaagacta ataaattag attaatact ttattttgg 
      gtgtttatt ccaagcttt agaattcat ttaacataa 
      atttttttt tatagagat tatgttcga ttagaacga 
      acaatcgct tattacgaa caaattcgt aagtccctt 
      gactgaagt ctaagctaa aacatctaa gaaaacaat 
      aataaaaac taaaataag acaaaaata atatttaga 
      aagaggaaa aaatatttt tagaaaatc ataaaaact 
      tacctattt acaaaaatt taaagaata aatttattc 
      taaaactaa atatgatta ccaaattgc attttagcg 
      ctttaacta acttaacta atttatttg caaaaatgt 
      cacattcat cttctaaac aactgcaac tcttatgtg 
      aaagtggtt aagaagctg attatggta gaactagct 
      tggtgaata atgacttta caataaagg aattttaaa 
      aatcgcctt tgttctgtt tctagcacc atcaaacca 
      acttgtaaa atggtgact aaattaagg agaaataaa 
      ttataaaca tttaattgc attgatgtt attaaaaga 
      tttaatatt tctaaataa aaagcctaa agttttgtg 
      gaccaaatt aacagaagc atttatgtg ggcatttat 
      ttaagtatt taaaaaata ttcccaaat ctttaaata 
      cgttttttc tttgatctc aagatagcg ttgaaataa 
      ttttgaagt tcgctgcaa tctgtcgtt gctaataaa 
      tttatacat ttataagta aatgtgcca ggagtagtg 
      gtagttttt tcttcctga caaacttcg aaaagtatc 
      taagatcag agtgtacag actgctgca cagcatctt 
      agtatgaat tacttgcta caattaaat tgattgtga 
      taaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY