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Gene information
General information
TC010238 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450712.1: 114163..125309
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010238
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04793

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010238 RA
      MFRFLDLVG VFIALFAIL FAYFKWTYQ YWSQKNVPF 
      IQPTIPFGN SNLFRQKES GGIRMKNFY DEMKAQGWK 
      HGGLYSILK PSYMVIDLD YLKNIMTKD FDYFSDRGF 
      YYNEKDDPL SAHLFAIGG EKWRNLRIK LTPTFTSGK 
      MKMMFQTLV DCAPGLVKQ IDRKMESID IKEVLGCFT 
      TDIIGSCAF GLDFNTSND NNSPFREYG RKAFVPTKF 
      DIVKIIFAM SFPRLAQLF RLTLTRKDV SEFFLKVVR 
      ETVEYREKN NYRRKDFIQ LLIDLKGED GKTLTINEI 
      AAQSFVFFI AGFETSSTT MAFVLYELS RRPDLQQKL 
      RDEINTVLS RYEGSITYE ATQEMKYMD QVINEALRM 
      YPPVPMLGR KCVKDYKIP DQDVIIEKG TSILIPVLG 
      IHYDQEYYP DPKTFDPER FNEENRKAR HHYAHLPFG 
      EGPRICIGM RFGLMQTKV GLATLLKNY KFKVGERTQ 
      EPLKFKVAS FVLAAEGEI WLDAEKL
    • >TC010238 RB
      MSLKTYTQI NHFLASLKP TTKKVVCVT HYTTLRSHR 
      MFRFLDLVG VFIALFAIL FAYFKWTYQ YWSQKNVPF 
      IQPTIPFGN SNLFRQKES GGIRMKNFY DEMKAQGWK 
      HGGLYSILK PSYMVIDLD YLKNIMTKD FDYFSDRGF 
      YYNEKDDPL SAHLFAIGG EKWRNLRIK LTPTFTSGK 
      MKMMFQTLV DCAPGLVKQ IDRKMESID IKEVLGCFT 
      TDIIGSCAF GLDFNTSND NNSPFREYG RKAFVPTKF 
      DIVKIIFAM SFPRLAQLF RLTLTRKDV SEFFLKVVR 
      ETVEYREKN NYRRKDFIQ LLIDLKGED GKTLTINEI 
      AAQSFVFFI AGFETSSTT MAFVLYELS RRPDLQQKL 
      RDEINTVLS RYEGSITYE ATQEMKYMD QVINEALRM 
      YPPVPMLGR KCVKDYKIP DQDVIIEKG TSILIPVLG 
      IHYDQEYYP DPKTFDPER FNEENRKAR HHYAHLPFG 
      EGPRICIGM RFGLMQTKV GLATLLKNY KFKVGERTQ 
      EPLKFKVAS FVLAAEGEI WLDAEKL
    CDS sequence
    • >TC010238 RA
      atgtttaga tttctcgac ctagtgggc gtgttcatt 
      gcactcttc gccattctc ttcgcttac tttaaatgg 
      acctaccaa tactggagt caaaaaaac gtccctttc 
      attcaacct accattcct tttggcaac tcaaacctt 
      ttccgccag aaggaaagt ggcggtatc cgcatgaaa 
      aacttctac gatgagatg aaagcacaa ggatggaaa 
      cacggaggt ctttactct attctcaaa cccagctat 
      atggttatc gatttggac tacttgaag aacatcatg 
      acgaaagat ttcgactat ttttccgac cgtggcttc 
      tactacaat gaaaaagac gaccctctt agtgcacat 
      ttatttgca atcggtggt gaaaagtgg cgcaactta 
      cgaattaaa ctaactccg actttcacc tccggaaaa 
      atgaaaatg atgtttcag actttggtc gactgtgcc 
      ccaggttta gtcaaacaa atcgatcga aaaatggaa 
      tcaattgat atcaaagaa gttttgggg tgtttcaca 
      actgatatc atcgggtca tgtgctttt gggcttgac 
      tttaacacc tcaaacgac aataattca ccattccgg 
      gagtatgga cgcaaagct ttcgttcca acaaaattc 
      gatatagtc aagataatt tttgcgatg agttttccg 
      cgacttgca caactgttc agattaaca ttgacacgg 
      aaagacgtt tcagaattt ttccttaaa gtagtgagg 
      gaaactgtt gaatatcgc gaaaaaaat aattacaga 
      cgcaaagat tttattcaa ttattgatt gacttgaag 
      ggtgaagac ggtaaaacc ctcacaatt aacgaaatc 
      gctgctcaa agctttgtg tttttcatt gccggtttt 
      gaaacatca tccacgacc atggccttt gtcctctac 
      gaattaagt cgaagacct gacttgcaa caaaagctc 
      agggatgaa attaacaca gttttaagt agatatgag 
      gggagtata acatacgaa gccacccaa gaaatgaaa 
      tatatggac caagtcata aacgaggct ttaaggatg 
      tacccacct gttcccatg cttggccgg aaatgtgtc 
      aaagactat aaaattccc gaccaggat gtaattatt 
      gaaaaaggg acatcaatc ctaatccca gttttggga 
      atccattac gaccaagaa tattatccg gatcctaaa 
      acttttgat cctgagagg tttaatgaa gagaataga 
      aaggcaagg catcattat gcacatctt ccgtttggt 
      gaagggcct aggatttgt atagggatg cggtttggt 
      ctaatgcag accaaagtt ggattggca acactgttg 
      aaaaactac aaatttaag gttggggaa aggacacaa 
      gaaccgttg aaattcaaa gttgcatcg tttgttttg 
      gcagctgaa ggagaaatt tggctcgat gctgaaaag 
      ttgtga
    • >TC010238 RB
      atgtcccta aaaacatac acacagatt aatcatttc 
      ctcgcgtca ttgaaacca acaacgaaa aaggtcgtg 
      tgcgtcaca cattacacc acattgaga tcacacaga 
      atgtttaga tttctcgac ctagtgggc gtgttcatt 
      gcactcttc gccattctc ttcgcttac tttaaatgg 
      acctaccaa tactggagt caaaaaaac gtccctttc 
      attcaacct accattcct tttggcaac tcaaacctt 
      ttccgccag aaggaaagt ggcggtatc cgcatgaaa 
      aacttctac gatgagatg aaagcacaa ggatggaaa 
      cacggaggt ctttactct attctcaaa cccagctat 
      atggttatc gatttggac tacttgaag aacatcatg 
      acgaaagat ttcgactat ttttccgac cgtggcttc 
      tactacaat gaaaaagac gaccctctt agtgcacat 
      ttatttgca atcggtggt gaaaagtgg cgcaactta 
      cgaattaaa ctaactccg actttcacc tccggaaaa 
      atgaaaatg atgtttcag actttggtc gactgtgcc 
      ccaggttta gtcaaacaa atcgatcga aaaatggaa 
      tcaattgat atcaaagaa gttttgggg tgtttcaca 
      actgatatc atcgggtca tgtgctttt gggcttgac 
      tttaacacc tcaaacgac aataattca ccattccgg 
      gagtatgga cgcaaagct ttcgttcca acaaaattc 
      gatatagtc aagataatt tttgcgatg agttttccg 
      cgacttgca caactgttc agattaaca ttgacacgg 
      aaagacgtt tcagaattt ttccttaaa gtagtgagg 
      gaaactgtt gaatatcgc gaaaaaaat aattacaga 
      cgcaaagat tttattcaa ttattgatt gacttgaag 
      ggtgaagac ggtaaaacc ctcacaatt aacgaaatc 
      gctgctcaa agctttgtg tttttcatt gccggtttt 
      gaaacatca tccacgacc atggccttt gtcctctac 
      gaattaagt cgaagacct gacttgcaa caaaagctc 
      agggatgaa attaacaca gttttaagt agatatgag 
      gggagtata acatacgaa gccacccaa gaaatgaaa 
      tatatggac caagtcata aacgaggct ttaaggatg 
      tacccacct gttcccatg cttggccgg aaatgtgtc 
      aaagactat aaaattccc gaccaggat gtaattatt 
      gaaaaaggg acatcaatc ctaatccca gttttggga 
      atccattac gaccaagaa tattatccg gatcctaaa 
      acttttgat cctgagagg tttaatgaa gagaataga 
      aaggcaagg catcattat gcacatctt ccgtttggt 
      gaagggcct aggatttgt atagggatg cggtttggt 
      ctaatgcag accaaagtt ggattggca acactgttg 
      aaaaactac aaatttaag gttggggaa aggacacaa 
      gaaccgttg aaattcaaa gttgcatcg tttgttttg 
      gcagctgaa ggagaaatt tggctcgat gctgaaaag 
      ttgtga
    mRNA sequence
    • >TC010238 RA
      aagtattat aatattgat tcattgttt caaatcaaa 
      tatagtata gcacagata acataaaca ttcgcctct 
      aaaatagct aacagtgtt tagaaaaac atacacaca 
      ggtttgcac aaccagtaa atgcttact tacttacca 
      tctcatcta acagattaa tcatttcct cgcgtcatt 
      gaaaccaac aacgaaaaa ggtcgtgtg cgtcacaca 
      ttacaccac attgagatc acacagaat gtttagatt 
      tctcgacct agtgggcgt gttcattgc actcttcgc 
      cattctctt cgcttactt taaatggac ctaccaata 
      ctggagtca aaaaaacgt ccctttcat tcaacctac 
      cattccttt tggcaactc aaacctttt ccgccagaa 
      ggaaagtgg cggtatccg catgaaaaa cttctacga 
      tgagatgaa agcacaagg atggaaaca cggaggtct 
      ttactctat tctcaaacc cagctatat ggttatcga 
      tttggacta cttgaagaa catcatgac gaaagattt 
      cgactattt ttccgaccg tggcttcta ctacaatga 
      aaaagacga ccctcttag tgcacattt atttgcaat 
      cggtggtga aaagtggcg caacttacg aattaaact 
      aactccgac tttcacctc cggaaaaat gaaaatgat 
      gtttcagac tttggtcga ctgtgcccc aggtttagt 
      caaacaaat cgatcgaaa aatggaatc aattgatat 
      caaagaagt tttggggtg tttcacaac tgatatcat 
      cgggtcatg tgcttttgg gcttgactt taacacctc 
      aaacgacaa taattcacc attccggga gtatggacg 
      caaagcttt cgttccaac aaaattcga tatagtcaa 
      gataatttt tgcgatgag ttttccgcg acttgcaca 
      actgttcag attaacatt gacacggaa agacgtttc 
      agaattttt ccttaaagt agtgaggga aactgttga 
      atatcgcga aaaaaataa ttacagacg caaagattt 
      tattcaatt attgattga cttgaaggg tgaagacgg 
      taaaaccct cacaattaa cgaaatcgc tgctcaaag 
      ctttgtgtt tttcattgc cggttttga aacatcatc 
      cacgaccat ggcctttgt cctctacga attaagtcg 
      aagacctga cttgcaaca aaagctcag ggatgaaat 
      taacacagt tttaagtag atatgaggg gagtataac 
      atacgaagc cacccaaga aatgaaata tatggacca 
      agtcataaa cgaggcttt aaggatgta cccacctgt 
      tcccatgct tggccggaa atgtgtcaa agactataa 
      aattcccga ccaggatgt aattattga aaaagggac 
      atcaatcct aatcccagt tttgggaat ccattacga 
      ccaagaata ttatccgga tcctaaaac ttttgatcc 
      tgagaggtt taatgaaga gaatagaaa ggcaaggca 
      tcattatgc acatcttcc gtttggtga agggcctag 
      gatttgtat agggatgcg gtttggtct aatgcagac 
      caaagttgg attggcaac actgttgaa aaactacaa 
      atttaaggt tggggaaag gacacaaga accgttgaa 
      attcaaagt tgcatcgtt tgttttggc agctgaagg 
      agaaatttg gctcgatgc tgaaaagtt gtgaagtac 
      tataaatgt cattttgat attgtgatt gttagcatt 
      tttccatat ttattactt gctttgtag tcctttatg 
      ttgtattag ttcttgtta tgaaaactt ttttcttta 
      aaatggtcg aggggcacc tattgacca aattttatt 
      tcaaaatac tgattattt gtaaagaaa atgaaaaac 
      ttataaaag taatagttt atgtggcga aaatgaatg 
      tttattttg ccgaatgca atgaggcaa ataatttag 
      ctgagccac ataaactca catttatgc cgaataaaa 
      tatactatt ttatccgca aacgtaatg gtgaagagt 
      aaagagcaa ttttaagca aaactaatt taaaggtaa 
      atgaataaa ataatcagg aagctttgc acgtggttc 
      attatcata acaatgctt ataagcaaa attcagtct 
      cgaattttt gtgacgaga ttttcgaat gtgtatttt 
      atactttaa ttagtgcag catctagtg cattgtctc 
      ccgtttcac aatgaggaa atcatcacc tctccagcg 
      aagcagcga aagacacaa gaaacaact gacaacatt 
      agaaaaaat cttgataac aggcaaaag taaaaatgc 
      tttatctcg taaaatgcc gaaaactag atcttttat 
      cagcgacac tcagtctca aatgttgtc acttgtgac 
      gagtgcagg taataacta ttttctatc agaggagat 
      tatttttgc ttatatatt aggatttta aaatttaaa 
      aaaatttgg gaacagtgt ttaagaaag taattctgt 
      aatg
    • >TC010238 RB
      tccaccctc gtgtcttca tttcttcgt agatatttt 
      gaaaagtca cagcaatat tttccttac gattaaaag 
      gacttgtga tgttgccat aaggaatgg tgggcttca 
      aatacggca actttttgc gttcccaat actgataaa 
      tccatttgt aatacgcga aggtaacag ttatcaaag 
      taataaata cactaatca cataaaaca ttttgattt 
      aattataac tggatgaat aacacaact cttgtgagc 
      aaaacacgg caagtattt ttaatctca agacaaaca 
      cagactttg atacattat tatactccc aaccgggga 
      taataacac gtctacttt ttacaagtc atcgtttga 
      cctgacgtc agtcagctg tttactgtc attttctaa 
      tgctaccaa tttaaaaaa tgtccctaa aaacataca 
      cacagatta atcatttcc tcgcgtcat tgaaaccaa 
      caacgaaaa aggtcgtgt gcgtcacac attacacca 
      cattgagat cacacagaa tgtttagat ttctcgacc 
      tagtgggcg tgttcattg cactcttcg ccattctct 
      tcgcttact ttaaatgga cctaccaat actggagtc 
      aaaaaaacg tccctttca ttcaaccta ccattcctt 
      ttggcaact caaaccttt tccgccaga aggaaagtg 
      gcggtatcc gcatgaaaa acttctacg atgagatga 
      aagcacaag gatggaaac acggaggtc tttactcta 
      ttctcaaac ccagctata tggttatcg atttggact 
      acttgaaga acatcatga cgaaagatt tcgactatt 
      tttccgacc gtggcttct actacaatg aaaaagacg 
      accctctta gtgcacatt tatttgcaa tcggtggtg 
      aaaagtggc gcaacttac gaattaaac taactccga 
      ctttcacct ccggaaaaa tgaaaatga tgtttcaga 
      ctttggtcg actgtgccc caggtttag tcaaacaaa 
      tcgatcgaa aaatggaat caattgata tcaaagaag 
      ttttggggt gtttcacaa ctgatatca tcgggtcat 
      gtgcttttg ggcttgact ttaacacct caaacgaca 
      ataattcac cattccggg agtatggac gcaaagctt 
      tcgttccaa caaaattcg atatagtca agataattt 
      ttgcgatga gttttccgc gacttgcac aactgttca 
      gattaacat tgacacgga aagacgttt cagaatttt 
      tccttaaag tagtgaggg aaactgttg aatatcgcg 
      aaaaaaata attacagac gcaaagatt ttattcaat 
      tattgattg acttgaagg gtgaagacg gtaaaaccc 
      tcacaatta acgaaatcg ctgctcaaa gctttgtgt 
      ttttcattg ccggttttg aaacatcat ccacgacca 
      tggcctttg tcctctacg aattaagtc gaagacctg 
      acttgcaac aaaagctca gggatgaaa ttaacacag 
      ttttaagta gatatgagg ggagtataa catacgaag 
      ccacccaag aaatgaaat atatggacc aagtcataa 
      acgaggctt taaggatgt acccacctg ttcccatgc 
      ttggccgga aatgtgtca aagactata aaattcccg 
      accaggatg taattattg aaaaaggga catcaatcc 
      taatcccag ttttgggaa tccattacg accaagaat 
      attatccgg atcctaaaa cttttgatc ctgagaggt 
      ttaatgaag agaatagaa aggcaaggc atcattatg 
      cacatcttc cgtttggtg aagggccta ggatttgta 
      tagggatgc ggtttggtc taatgcaga ccaaagttg 
      gattggcaa cactgttga aaaactaca aatttaagg 
      ttggggaaa ggacacaag aaccgttga aattcaaag 
      ttgcatcgt ttgttttgg cagctgaag gagaaattt 
      ggctcgatg ctgaaaagt tgtgaagta ctataaatg 
      tcattttga tattgtgat tgttagcat ttttccata 
      tttattact tgctttgta gtcctttat gttgtatta 
      gttcttgtt atgaaaact tttttcttt aaaatggtc 
      gaggggcac ctattgacc aaattttat ttcaaaata 
      ctgattatt tgtaaagaa aatgaaaaa cttataaaa 
      gtaatagtt tatgtggcg aaaatgaat gtttatttt 
      gccgaatgc aatgaggca aataattta gctgagcca 
      cataaactc acatttatg ccgaataaa atatactat 
      tttatccgc aaacgtaat ggtgaagag taaagagca 
      attttaagc aaaactaat ttaaaggta aatgaataa 
      aataatcag gaagctttg cacgtggtt cattatcat 
      aacaatgct tataagcaa aattcagtc tcgaatttt 
      tgtgacgag attttcgaa tgtgtattt tatacttta 
      attagtgca gcatctagt gcattgtct cccgtttca 
      caatgagga aatcatcac ctctccagc gaagcagcg 
      aaagacaca agaaacaac tgacaacat tagaaaaaa 
      tcttgataa caggcaaaa gtaaaaatg ctttatctc 
      gtaaaatgc cgaaaacta gatctttta tcagcgaca 
      ctcagtctc aaatgttgt cacttgtga cgagtgcag 
      gtaataact attttctat cagaggaga ttatttttg 
      cttatatat taggatttt aaaatttaa aaaaatttg 
      ggaacagtg tttaagaaa gtaattctg taatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY