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Gene information
General information
TC010234 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451053.1: 17001..38153
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010234
    iBeetle screen iB_04791

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larva segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head capsule not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    labrum not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    antenna not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    mandible not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    mesothorax not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    mesothoracic leg femur duplicated - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs partially shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen complete not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 9 (16 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010234 RA
      MINAFMDTG HHHHLASYG LKMSPGHQA QTPAAAMPA 
      PDQNHHFQN YGHMGHMGG YAARDFLLR REDFPPQTA 
      PSEPVLFHH HGDVPQHHL PHHHQMLYS RPDAYHQPQ 
      HQYLNPMGV PAHHSHGAF FRYMRQPIK QEMQCLWVD 
      PEQPPPRKI CAKHFTSMH EIVTHLTVE HVGGPECTT 
      HACFWQNCS RNGRPFKAK YKLVNHIRV HTGEKPFPC 
      PFPGCGKVF ARSENLKIH KRTHTGEKP FKCEYEGCD 
      RRFANSSDR KKHSHVHTS DKPYNCRVS GCDKSYTHP 
      SSLRKHMKV HGCSGRSPP HYDSDGEES NSSSAGSIS 
      VAASPQVSG PQVPPNTLS EWYVACQPP PPVTPDPLG 
      GLGHFGNLH HSGAATAY
    CDS sequence
    • >TC010234 RA
      atgatcaac gccttcatg gacacgggc caccaccac 
      cacctcgcc tcctacggc ctgaagatg agccccggc 
      caccaggcc cagacgccg gcggcggcg atgcccgcc 
      cccgaccag aaccaccac ttccagaac tacggccac 
      atgggccac atggggggc tacgccgcg cgggacttc 
      ctgctgcgg agggaggac ttcccgccg cagacggcg 
      cccagcgag ccggtgctc ttccaccac cacggcgac 
      gtgccccag caccacctg ccgcaccac caccagatg 
      ctctactcg cgccccgac gcctaccac cagccccag 
      caccagtac ctcaacccc atgggggtg cccgcgcac 
      cactcgcac ggcgccttc ttcaggtac atgcgccag 
      cccatcaag caggagatg cagtgtctg tgggtggac 
      cccgagcag ccgccgccg aggaagatc tgcgccaag 
      cacttcacc agcatgcac gagatcgtc acccacctc 
      acggtggag cacgtcgga gggcccgag tgcaccacg 
      catgcctgc ttctggcag aactgctcc aggaacggg 
      aggcccttc aaagccaaa tataaatta gtcaatcat 
      ataagggtg cacacgggg gagaagccc tttccgtgc 
      ccgtttcca ggctgtggc aaggttttc gcgaggagc 
      gagaacctg aagattcac aagaggacg cacacaggc 
      gagaagccg ttcaaatgc gagtacgaa gggtgcgac 
      cgacgcttc gccaactcc tcggaccgg aaaaagcac 
      tcgcacgtc cacacctcc gacaagccc tacaactgc 
      cgcgtctcc ggctgcgac aagtcctac acccacccc 
      tcgtcgctc cgcaagcac atgaaggtg cacggctgc 
      tccgggcgc tcgcccccg cactacgac agcgacggc 
      gaggagtcc aactcctcc tcggcgggc agcatctcg 
      gtggcggcc agcccgcag gtctccggc ccgcaggtg 
      ccccccaac acgctgagc gagtggtac gtggcgtgc 
      cagccgccg cccccggtc acgcccgac ccgctgggc 
      ggcctgggg cacttcggg aatctgcac cacagtggc 
      gccgccacc gcctattag
    mRNA sequence
    • >TC010234 RA
      cagttcccc aagaatggc ctactgcgc gctcagtcc 
      agctgtgat caccgtccg tggcagttg tttgcgcgt 
      cttcggtat catgtgttc agtgacctt cttgtagtt 
      gtttttccg accgcttcc acgatgatc aacgccttc 
      atggacacg ggccaccac caccacctc gcctcctac 
      ggcctgaag atgagcccc ggccaccag gcccagacg 
      ccggcggcg gcgatgccc gcccccgac cagaaccac 
      cacttccag aactacggc cacatgggc cacatgggg 
      ggctacgcc gcgcgggac ttcctgctg cggagggag 
      gacttcccg ccgcagacg gcgcccagc gagccggtg 
      ctcttccac caccacggc gacgtgccc cagcaccac 
      ctgccgcac caccaccag atgctctac tcgcgcccc 
      gacgcctac caccagccc cagcaccag tacctcaac 
      cccatgggg gtgcccgcg caccactcg cacggcgcc 
      ttcttcagg tacatgcgc cagcccatc aagcaggag 
      atgcagtgt ctgtgggtg gaccccgag cagccgccg 
      ccgaggaag atctgcgcc aagcacttc accagcatg 
      cacgagatc gtcacccac ctcacggtg gagcacgtc 
      ggagggccc gagtgcacc acgcatgcc tgcttctgg 
      cagaactgc tccaggaac gggaggccc ttcaaagcc 
      aaatataaa ttagtcaat catataagg gtgcacacg 
      ggggagaag ccctttccg tgcccgttt ccaggctgt 
      ggcaaggtt ttcgcgagg agcgagaac ctgaagatt 
      cacaagagg acgcacaca ggcgagaag ccgttcaaa 
      tgcgagtac gaagggtgc gaccgacgc ttcgccaac 
      tcctcggac cggaaaaag cactcgcac gtccacacc 
      tccgacaag ccctacaac tgccgcgtc tccggctgc 
      gacaagtcc tacacccac ccctcgtcg ctccgcaag 
      cacatgaag gtgcacggc tgctccggg cgctcgccc 
      ccgcactac gacagcgac ggcgaggag tccaactcc 
      tcctcggcg ggcagcatc tcggtggcg gccagcccg 
      caggtctcc ggcccgcag gtgcccccc aacacgctg 
      agcgagtgg tacgtggcg tgccagccg ccgcccccg 
      gtcacgccc gacccgctg ggcggcctg gggcacttc 
      gggaatctg caccacagt ggcgccgcc accgcctat 
      tagtgatcg ttgagttgt gtcttgtac atagaggcg 
      gccgggaca attgacaaa gaactacac gtttggata 
      tttaaggcc cggttttgc attttgact gtgaatatt 
      ttatttaat tatttaatg ttttaaatg gttcccgtg 
      caattacta ctggtatac agttgctga gttttcttc 
      gtatcatga gttccgaac tattggata agtttttat 
      gataatgcg tatttgtat atttgtata aaactttat 
      tattgatat ttaattgta ggaccacaa ttgtataaa 
      gagaaatta ttgtacata tatcttatc agggatctc 
      tgtatatct aaagtttat tataacttg aatttattg 
      aaaataaat ctgactgat gatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY