Status
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Gene information
General information
TC031215
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010221,TC010222
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 16143607..16169746
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031215
    iBeetle screen iB_04789

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 6 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031215 RA
      MFLNYVIAA VSVLLYLHG FFPIPTRPI EKAPPSNRT 
      HTPNAKKLV LVVIDALRL DFISATKTP FLSKSLRNN 
      GCFIHLKVE TPTVTLPRI KALTTGNVP QFVDIILNL 
      ANPTKVEDS FIHRAHAAG KKIVFYGDD IWVKLFSDE 
      FVRSEGTSS FFVNDFTEV DDNVTRNVK LEVKRSDWD 
      IMILHYLGL DHIGHVYGP KSPLILSKL KEMDYVIEE 
      IYKTDAILM VTGDHGMRD SGGHGGSTH PETNVPFIV 
      LGVPCINDS FAQIDIPAN LAILQNFDV PTTSIGQLH 
      KSLLSHLQQ SEFLQALRY NTFLLTNKL DLCEETIHT 
      ADHLFDLFL LNQKKELAE SAAQLYENC ARKISETLQ 
      KLSIQQNAA SLIVATATT VVILLKVIQ KLFGVSPKT 
      DRFEQLTML ALLFLQFAH FHVAMDMLL TVTILFLVV 
      RNLACFETK HLQCANKSF LMFMSIVHP LSFLSSSYL 
      EEEHQFWEI LDFAFCSTQ TRSHT
    • >TC031215 RB
      MYTLLTLIE SCMNSRPIT SLSNDPNDL EPLTPGHWG 
      PLDGAVRNS HVVAAGQPP VQVTIGGKN KADILEKME 
      ERISVAVPS QEQVEDSID YFLATGHLG DFVGGQPAS 
      LTLAVRTHH RNPRGRRRH HQGRISEDR EWNHKKRSL 
      DHIGHVYGP KSPLILSKL KEMDYVIEE IYKTDAILM 
      VTGDHGMRD SGGHGGSTH PETNVPFIV LGVPCINDS 
      FAQIDIPAN LAILQNFDV PTTSIGQLH KSLLSHLQQ 
      SEFLQALRY NTFLLTNKL DLCEETIHT ADHLFDLFL 
      LNQKKELAE SAAQLYENC ARKISETLQ KLSIQQNAA 
      SLIVATATT VVILLKVIQ KLFGVSPKT DRFEQLTML 
      ALLFLQFAH FHVAMDMLL TVTILFLVV RNLACFETK 
      HLQCANKSF LMFMSIVHP LSFLSSSYL EEEHQFWEI 
      LDFAFCSTQ TRSHT
    CDS sequence
    • >TC031215 RA
      atgttcctc aattatgtc attgccgcc gtctccgtg 
      ctcctctac ttgcacgga ttttttcca atcccgacc 
      cggcccatt gaaaaggcg ccccccagc aacagaacc 
      catactcca aacgccaag aaattggtc ttggtagtg 
      attgacgcc ctacgtctc gacttcatt tccgcaacc 
      aaaacgcca ttcttatca aaatcatta agaaataat 
      ggctgtttt attcatctc aaagttgaa acaccaact 
      gttacacta ccacggata aaagcgctg acgacagga 
      aatgtgccg caatttgtc gatattatc ctgaatttg 
      gccaatccg acaaaagtt gaggacagt tttattcat 
      cgagctcat gccgccggc aagaaaatt gttttttat 
      ggtgatgat atttgggtg aaacttttt tctgatgag 
      tttgtcagg agtgaaggc acttcgtca ttttttgtt 
      aatgatttt acggaagtg gatgataat gtgacgaga 
      aatgttaaa ctggaagtc aagaggagc gattgggac 
      atcatgatt ttgcactat ttaggtttg gatcatata 
      ggccacgtc tacggtccc aaatctccg cttatttta 
      tccaaactc aaagaaatg gattacgtt atagaagaa 
      atctacaaa accgacgcg attttgatg gtaacaggt 
      gatcacgga atgagagac agcggaggg catgggggc 
      tccacacat cctgaaacc aacgtcccc tttatagtt 
      ttgggtgtg ccttgcatt aatgactct tttgcccaa 
      attgacatt cccgctaat ttagccatt ttacaaaat 
      ttcgatgtt ccaaccact agcattggc caacttcac 
      aaaagtctg ttgtctcat ttgcaacaa tcagaattt 
      ttacaagct ttgagatac aacaccttt ttattgaca 
      aataagctg gatttgtgc gaagaaaca attcacact 
      gcagaccat cttttcgat ctatttcta cttaatcag 
      aaaaaagaa ttggccgaa agcgcggcc caattgtac 
      gaaaactgt gcacgaaaa attagcgaa acgttacaa 
      aaactttca attcaacaa aacgcagca tctctgatt 
      gtcgcaact gctacaacc gttgtaatt ttgttaaaa 
      gtaatccaa aaactgttc ggggtttct ccgaaaact 
      gatcgcttc gaacaatta acaatgttg gcactgcta 
      tttttacag tttgcccat tttcacgtt gccatggat 
      atgttactt accgtgaca atattattt ttagttgtt 
      agaaatctt gcgtgtttt gagacaaaa catttgcag 
      tgcgctaac aaatctttt ttgatgttt atgtcaatt 
      gtgcatccg ttgtctttc ctctcctcg agttacttg 
      gaagaagag caccaattt tgggaaata ctggatttt 
      gctttttgt agcactcag actcgttcg cacacttaa
    • >TC031215 RB
      atgtacacc ttgctgaca ttaatagaa tcatgcatg 
      aactcccga cctattaca tctttgagt aacgaccca 
      aatgacttg gaaccattg acgccgggc cattggggc 
      ccccttgac ggcgccgtt agaaactca catgttgtc 
      gctgccgga caaccgcct gtccaggtg acaattggt 
      ggaaaaaat aaggcagac attttggaa aagatggag 
      gagcgaata tctgtcgca gttccaagt caggaacaa 
      gtggaagac agcatcgac tatttcctt gcaaccggg 
      caccttggt gattttgtg ggaggacaa cctgcctcc 
      cttacactg gcagttagg acgcatcac agaaaccca 
      cgagggcgc agacggcat caccagggt cgtatcagt 
      gaagaccgc gagtggaat cacaaaaag aggagtttg 
      gatcatata ggccacgtc tacggtccc aaatctccg 
      cttatttta tccaaactc aaagaaatg gattacgtt 
      atagaagaa atctacaaa accgacgcg attttgatg 
      gtaacaggt gatcacgga atgagagac agcggaggg 
      catgggggc tccacacat cctgaaacc aacgtcccc 
      tttatagtt ttgggtgtg ccttgcatt aatgactct 
      tttgcccaa attgacatt cccgctaat ttagccatt 
      ttacaaaat ttcgatgtt ccaaccact agcattggc 
      caacttcac aaaagtctg ttgtctcat ttgcaacaa 
      tcagaattt ttacaagct ttgagatac aacaccttt 
      ttattgaca aataagctg gatttgtgc gaagaaaca 
      attcacact gcagaccat cttttcgat ctatttcta 
      cttaatcag aaaaaagaa ttggccgaa agcgcggcc 
      caattgtac gaaaactgt gcacgaaaa attagcgaa 
      acgttacaa aaactttca attcaacaa aacgcagca 
      tctctgatt gtcgcaact gctacaacc gttgtaatt 
      ttgttaaaa gtaatccaa aaactgttc ggggtttct 
      ccgaaaact gatcgcttc gaacaatta acaatgttg 
      gcactgcta tttttacag tttgcccat tttcacgtt 
      gccatggat atgttactt accgtgaca atattattt 
      ttagttgtt agaaatctt gcgtgtttt gagacaaaa 
      catttgcag tgcgctaac aaatctttt ttgatgttt 
      atgtcaatt gtgcatccg ttgtctttc ctctcctcg 
      agttacttg gaagaagag caccaattt tgggaaata 
      ctggatttt gctttttgt agcactcag actcgttcg 
      cacacttaa
    mRNA sequence
    • >TC031215 RA
      tatcaacaa aattatccg tttaatcac aataataaa 
      ggatactta ccgcagtta tcaaaatac cttctaaaa 
      tgcttaaaa atcgcagca agtatgtat ccgccgaat 
      taatacagt aacagtgaa agtaaatta ataacttaa 
      taagtgaaa actgacaac caactgtgt caacaaaca 
      gtaatgttc ctcaattat gtcattgcc gccgtctcc 
      gtgctcctc tacttgcac ggatttttt ccaatcccg 
      acccggccc attgaaaag gcgcccccc agcaacaga 
      acccatact ccaaacgcc aagaaattg gtcttggta 
      gtgattgac gccctacgt ctcgacttc atttccgca 
      accaaaacg ccattctta tcaaaatca ttaagaaat 
      aatggctgt tttattcat ctcaaagtt gaaacacca 
      actgttaca ctaccacgg ataaaagcg ctgacgaca 
      ggaaatgtg ccgcaattt gtcgatatt atcctgaat 
      ttggccaat ccgacaaaa gttgaggac agttttatt 
      catcgagct catgccgcc ggcaagaaa attgttttt 
      tatggtgat gatatttgg gtgaaactt ttttctgat 
      gagtttgtc aggagtgaa ggcacttcg tcatttttt 
      gttaatgat tttacggaa gtggatgat aatgtgacg 
      agaaatgtt aaactggaa gtcaagagg agcgattgg 
      gacatcatg attttgcac tatttaggt ttggatcat 
      ataggccac gtctacggt cccaaatct ccgcttatt 
      ttatccaaa ctcaaagaa atggattac gttatagaa 
      gaaatctac aaaaccgac gcgattttg atggtaaca 
      ggtgatcac ggaatgaga gacagcgga gggcatggg 
      ggctccaca catcctgaa accaacgtc ccctttata 
      gttttgggt gtgccttgc attaatgac tcttttgcc 
      caaattgac attcccgct aatttagcc attttacaa 
      aatttcgat gttccaacc actagcatt ggccaactt 
      cacaaaagt ctgttgtct catttgcaa caatcagaa 
      tttttacaa gctttgaga tacaacacc tttttattg 
      acaaataag ctggatttg tgcgaagaa acaattcac 
      actgcagac catcttttc gatctattt ctacttaat 
      cagaaaaaa gaattggcc gaaagcgcg gcccaattg 
      tacgaaaac tgtgcacga aaaattagc gaaacgtta 
      caaaaactt tcaattcaa caaaacgca gcatctctg 
      attgtcgca actgctaca accgttgta attttgtta 
      aaagtaatc caaaaactg ttcggggtt tctccgaaa 
      actgatcgc ttcgaacaa ttaacaatg ttggcactg 
      ctattttta cagtttgcc cattttcac gttgccatg 
      gatatgtta cttaccgtg acaatatta tttttagtt 
      gttagaaat cttgcgtgt tttgagaca aaacatttg 
      cagtgcgct aacaaatct tttttgatg tttatgtca 
      attgtgcat ccgttgtct ttcctctcc tcgagttac 
      ttggaagaa gagcaccaa ttttgggaa atactggat 
      tttgctttt tgtagcact cagactcgt tcgcacact 
      taaccaagt tggtgataa atgggctgc attgccgga 
      tttagctga ctggttgtt acaaaacga aaattattt 
      atatttgca agtaatttt tatttcggg tttagcagc 
      ctcgtacct ttgtaccat tatgtttgc aaaaacgaa 
      attgagtaa gtttcttga tgctttagt cttaatttt 
      aatttatgt ctttcgctc agtgttcgt gaatataat 
      cttgggcca aattttgtg gtttctaat ttttataaa 
      atttctact ttgtgtatc aaccaaaga aagttttgc 
      attagtttg gttactact tattagttt gttacttaa 
      gccctacaa tgtgatact tattccatt gtgtgtatt 
      aggtagtca acttttcca caaagcttt agaaaatga 
      aacgttaac gatggccca ttggtggtt aggtaattt 
      gttattttt tgctcaagg gcatgataa tagtttagc 
      aagtgttga tatttcaag cggttatat tggattaca 
      cgagtataa ccctttttt agtctttcc tcaagtatt 
      gtgtcacac atatgccct cccagtatt aagccactt 
      attactttt cacaaacag aaaggtaaa tgtttttaa 
      aagtctgtc tatttttat attgtttag attatatat 
      tgttgtgct agtttttat agtaacttt catacacag 
      acaccattt gtttatttg gtcagtttt tgccccaaa 
      aatgtttgt tgaggcaag ccatttttt ctttttatt 
      tgttgaagt atcttgcta ctatttaat agatttaat 
      aaaaaaaaa ttgttactg aaattgtgt ttttttaat 
      aaaacatct gttacaaa
    • >TC031215 RB
      ctcgaagag caaatagag gaacgcgag tcatatggg 
      aaacatttt tatgagacg ttaatcgcg gcagaagaa 
      ataataaac aaacgggaa aataacgca aaggaggac 
      tcatctccc tcaggtggc caatcgttt gttcaacta 
      ccgtcaatc aatttaccg tcgtttagt ggccaatat 
      gtggaatgg acgaatttt catgatagt tttacagtc 
      ttgattcat aataataca gcgttaggg aatatagaa 
      aagtttcat tatttgaaa aattgtcta acagggtta 
      aaaacaggg tttgactag tcaggaaac gcgtctcca 
      aaatacgca tttagggtt tgtaatgga tgggccact 
      tacaatcgc tgctttaga gcatcattc tcgtaatca 
      aatgtgtca tgtatcaaa ctcgaattt ggaaaagca 
      cgtcataaa attctggga gttagaaaa tttcgaaaa 
      cgtgaaggg cacaacagc tgaagaaaa atcgtgtga 
      atctcattt tgtgaaaac tcattgtag aaataagga 
      tggccgggc cggttatag ttaccgcaa aaattattt 
      tacaaaaat tatggctac taaaaatta attgggacg 
      atccaattc cccaaacat tacgaagta gattttgta 
      tgttcttag aagaattac accttatta gtagttaca 
      atatttcgc gaaaagtta ttccttttt ttccatata 
      aaactattc aattacacg gattttgcg atgctagcc 
      tagaagcgt atggtgtag tgatttata tcagaacaa 
      cagatttag atggtgaag tggcaacgc gtcttttgt 
      gtgcgaaat cgcgcgtag tgccgctta agcaaataa 
      cgttaccac gattagaat taagcgcag cggtacttt 
      tggctaaac tctttgaga aagtcataa ccacagtag 
      gggtaacat ttgacaaat attttttat ggtcggatt 
      caacaataa cgttagctt ggatcaaag gagcaccaa 
      gtaagtggc aacaatttg tggcatctc gggtcggtc 
      taatccaat caaatacgg aaatggcta actgggttc 
      atgtaaatt caggtgata accccgctg atattattt 
      cgagaggcg caagtcctc aaacgttga aaagttcgt 
      cgttgtggt ggaacggac ccacaaggt taaacaaaa 
      atgagcatg agtggccaa agtccaatg taaatgtgt 
      caaaaattg aagctccag agcaaaaaa gcataagct 
      taattaatt gtagcgaca cgtcggagt taccaattt 
      ttgctaaag tgtcctcat tttcaaaat taataaaca 
      tcatgggct actgtttaa gattcgcta ataattgtc 
      gtaataaaa cggctaaac attctgaaa tattgctga 
      cacgacaag aaagagatg cggcttgta acactattg 
      taaaattgg tacaattaa gaatatttc gcaggacag 
      taacagagt taaaaaatg ataaagaag tcagtaaag 
      aaaataatt tacgatcac ttaacttat ttctagaca 
      gtaatgaaa ttattagag ttggtggcc gtctaagga 
      atgcaagaa tacaatttg atagtaaac accaaatgt 
      tattaccaa aagatcata tagttacaa aattaataa 
      ttcaataca ttcacgaga agaatttgc acgcgggaa 
      ctcaagcaa cgttagcag cgataagac aaaagtact 
      ggccaatct cgggacgca gtgtagttc gtcaagtaa 
      tacataagt gcataattt gttttaaac taaaacctt 
      tgccttttg aacaactaa tgggagatt taccaaccg 
      aaagggtta ctcctgcgc gaccattcg cgaattgtg 
      gggtcgatt atgcaggcc ctatactaa ttaaagaag 
      cgcgaggct gaggcaaaa gaactgtaa agtcatatg 
      tagcgttgt ttatttgtt tggtaacga gagcagtcc 
      atctagaat tggtccttg attatacct cggagtaat 
      tttgaatgc cttaaaacg catgatggc acgacgcgg 
      taaagtaga gtcaattat atcggacaa tggtacaaa 
      tttcgtggg tgcaaacag agagcgttg caggaaatt 
      gaacattta tttaagtca aatgaattt gaaaagaaa 
      gtggtcaaa gaattaaat aatgaaggt attaaatga 
      aagtttatt cctgccaag tctccatat atcggggga 
      atttgggaa gcgggggtt aaagcattt aagcatcat 
      attaaacgt gttgtggga ctctcgtta ttaacatat 
      gaagaaatg tacaccttg ctgacatta atagaatca 
      tgcatgaac tcccgacct attacatct ttgagtaac 
      gacccaaat gacttggaa ccattgacg ccgggccat 
      tggggcccc cttgacggc gccgttaga aactcacat 
      gttgtcgct gccggacaa ccgcctgtc caggtgaca 
      attggtgga aaaaataag gcagacatt ttggaaaag 
      atggaggag cgaatatct gtcgcagtt ccaagtcag 
      gaacaagtg gaagacagc atcgactat ttccttgca 
      accgggcac cttggtgat tttgtggga ggacaacct 
      gcctccctt acactggca gttaggacg catcacaga 
      aacccacga gggcgcaga cggcatcac cagggtcgt 
      atcagtgaa gaccgcgag tggaatcac aaaaagagg 
      agtttggat catataggc cacgtctac ggtcccaaa 
      tctccgctt attttatcc aaactcaaa gaaatggat 
      tacgttata gaagaaatc tacaaaacc gacgcgatt 
      ttgatggta acaggtgat cacggaatg agagacagc 
      ggagggcat gggggctcc acacatcct gaaaccaac 
      gtccccttt atagttttg ggtgtgcct tgcattaat 
      gactctttt gcccaaatt gacattccc gctaattta 
      gccatttta caaaatttc gatgttcca accactagc 
      attggccaa cttcacaaa agtctgttg tctcatttg 
      caacaatca gaattttta caagctttg agatacaac 
      accttttta ttgacaaat aagctggat ttgtgcgaa 
      gaaacaatt cacactgca gaccatctt ttcgatcta 
      tttctactt aatcagaaa aaagaattg gccgaaagc 
      gcggcccaa ttgtacgaa aactgtgca cgaaaaatt 
      agcgaaacg ttacaaaaa ctttcaatt caacaaaac 
      gcagcatct ctgattgtc gcaactgct acaaccgtt 
      gtaattttg ttaaaagta atccaaaaa ctgttcggg 
      gtttctccg aaaactgat cgcttcgaa caattaaca 
      atgttggca ctgctattt ttacagttt gcccatttt 
      cacgttgcc atggatatg ttacttacc gtgacaata 
      ttattttta gttgttaga aatcttgcg tgttttgag 
      acaaaacat ttgcagtgc gctaacaaa tcttttttg 
      atgtttatg tcaattgtg catccgttg tctttcctc 
      tcctcgagt tacttggaa gaagagcac caattttgg 
      gaaatactg gattttgct ttttgtagc actcagact 
      cgttcgcac acttaacca agttggtga taaatgggc 
      tgcattgcc ggatttagc tgactggtt gttacaaaa 
      cgaaaatta tttatattt gcaagtaat ttttatttc 
      gggtttagc agcctcgta cctttgtac cattatgtt 
      tgcaaaaac gaaattgag taagtttct tgatgcttt 
      agtcttaat tttaattta tgtctttcg ctcagtgtt 
      cgtgaatat aatcttggg ccaaatttt gtggtttct 
      aatttttat aaaatttct actttgtgt atcaaccaa 
      agaaagttt tgcattagt ttggttact acttattag 
      tttgttact taagcccta caatgtgat acttattcc 
      attgtgtgt attaggtag tcaactttt ccacaaagc 
      tttagaaaa tgaaacgtt aacgatggc ccattggtg 
      gttaggtaa tttgttatt ttttgctca agggcatga 
      taatagttt agcaagtgt tgatatttc aagcggtta 
      tattggatt acacgagta taacccttt tttagtctt 
      tcctcaagt attgtgtca cacatatgc cctcccagt 
      attaagcca cttattact tttcacaaa cagaaaggt 
      aaatgtttt taaaagtct gtctatttt tatattgtt 
      tagattata tattgttgt gctagtttt tatagtaac 
      tttcataca cagacacca tttgtttat ttggtcagt 
      ttttgcccc aaaaatgtt tgttgaggc aagccattt 
      tttcttttt atttgttga agtatcttg ctactattt 
      aatagattt aataaaaaa aaattgtta ctgaaattg 
      tgttttttt aataaaaca tctgttaca aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY