Status
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Gene information
General information
TC010212 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 15646160..15650301
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC010212
      Gene ontology

      GO terms for Tribolium

      • {{ goTerm.term }}
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        Status: {{ goTerm.status }}
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        Quotation: {{ goTerm.quotation }}
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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
      {{ blurIdResult }}
      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
      {{ blurEvidenceResult }}
      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
        {{ unavailableText }}

      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_04788

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection


      iBeetle screen iB_07723

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC010212 RA
        MPDNPPDFN AAVQVEIER VTVKPPPFW KGDPKLWFI 
        QLEAQFNLG KITSDTTKY HYVVSAIDT SVLQQIAAF 
        VTNPPAVNQ YEGIKQRLI STFSDSQER QLRKLLSEI 
        DLGDKKPSQ LLNEMKRLG DSAVSDELL KTLWLQRLP 
        THVQSVLAT SSDPIQNLA QMADKIVEI QHMSSVCTV 
        TTPTEDMSA AILQLTKEV AALKAAYNT EIKPRRSRS 
        RPQTPDRSS RDSCWYHRT FGNKAKKCI TPCNFNKSE 
        NSTPRHRRL TITDPTTGI SFLIDSGSD ISIIPKKAR 
        QSTVSDLVL YAANGTAIQ TYGYERLTI TLGLRRRLI 
        WNFIIADTK RAIIGADLL HYFDLLIDI RRARLIDST 
        TGISSAGTC ISASHTAVL SVSPNARYR DLIREFPAL 
        TTFHNVEPI LPHATQHLI ETTGPPVAS KPRRLPPDK 
        LQIAKREFE HMMALGICR PSNSPWASP LHMVPKKDS 
        NWRPVGDYR RLNAVTKED RYPIPHLHD FAHLLAGKT 
        IFSTVDLVR AYHQIPVEA SSIPKTAIT TPFGLFEFT 
        RMQFGLRNA AQSFQRFIH EVLNGLDFC FPYLDDILI 
        ASTSEREHT DHLRKVFER LLKYGLTIN PEKCSFGNS 
        KVNFLGYEV SADGTKPLT DRVKVILNY PLPKTSKDA 
        RRFLAAHQA PIHNLVKNS KKNDKTPLS WTPESKAAF 
        ELCKHDLAQ ATLLVHPTT TDTISLTVD ASDFAMRAV 
        LEQNQGGSW KPLSFFSKK LTPAQQKYS TYDRELLAI 
        YSAVKAFQH FLEGRHFVI YTDHKPLVY AFTQKSDKA 
        TPRQARHLD YISQFTTVI NHISGKSNV VADTLSRIS 
        EIDTPTPID YQTLRESQD VDPELQTLL AQPDSTALK 
        LKLFTVPGS EKQIYCDDS NRRIRPFIP KTSRYELFK 
        HFHGMAHPG IKATTKLLT DRFVWPGIN KDVSRWTRA 
        CINCQRSKV QRHTQPPIT EIGTSDERF AVINIDLIG 
        PLPPSNGFT YCLTCIDRY TSWTEAIPL TDITAESVA 
        KALYSGWIS RFGTPLKII TDQGRQFES SLFASLSTL 
        MGFRRARCT PYHPATNGK IERWHRTLK TAIKAHASP 
        NWTEHLPTV LLGLRTVIR DDTPVSASE MVYGSTIRL 
        PGEFFQDST NTIDPATFV GQLKANIAN VRPASAPHH 
        DNRQIFVPQ NLDTCTHVF VRRDAVRKP FDPPYDGPC 
        KVLNRTEKY FTVDLNNKS TNISLSRLK PAFLLNDNP 
        IRHDHTYAN WAITPEKTD LKKTVRFKL
      CDS sequence
      • >TC010212 RA
        atgcccgat aacccacca gatttcaac gccgctgtc 
        caggtcgag atcgagagg gttactgtt aaaccgcca 
        cccttttgg aaaggtgat cccaagctg tggtttatc 
        caacttgaa gcccaattc aacctcggc aagataaca 
        agcgataca actaagtat cactacgtg gtcagtgct 
        atcgacaca tcagttctt cagcaaatt gccgctttc 
        gtgaccaat cctcccgct gtcaaccaa tatgaaggt 
        atcaaacaa cggcttatt tctacattt tcggattct 
        caggaacgc caattgcgt aaactactg tcggaaata 
        gacttaggc gacaagaaa ccttcgcaa ttgcttaac 
        gaaatgaag cgtctgggt gattcggct gtatccgac 
        gaattactg aaaactttg tggttacaa agattgccg 
        acccacgtc caatccgtg ctcgcaacc agctccgac 
        ccgattcag aaccttgcg caaatggca gacaaaatc 
        gtggagata caacacatg tcctcagtt tgtacagtt 
        acgacccca accgaagat atgtctgca gctatcctg 
        caactcact aaggaagtc gcagcatta aaggctgcc 
        tacaatact gaaattaag ccgcgacga tcacgatca 
        cgaccacag acgccagac cgttcaagc agggactct 
        tgttggtac caccgaaca tttggcaat aaagcaaag 
        aagtgtatc actccctgc aacttcaac aagtcggaa 
        aactccacg ccgcgccat cgacgcctc accataacc 
        gatccaacc accggtatt tcgtttttg attgactct 
        ggttcggat atttcaatc atccccaag aaagcacga 
        cagtcaaca gtaagtgac ctcgtactt tacgctgcc 
        aatggcacc gccattcaa acctacggt tacgagaga 
        cttaccatt actttggga ttacgacgt cgattaatt 
        tggaacttc atcatcgct gataccaaa cgagcaata 
        attggcgcc gatttatta cattatttc gatctttta 
        atcgatatt agacgggcc agactaatt gatagcacg 
        acaggaatc tccagcgca ggcacatgc atttccgcc 
        tcgcacact gcagtgttg tcagtttca ccgaacgct 
        cggtatcgc gacctgatc agggaattt ccagctctg 
        acaactttt cacaatgtc gaaccaatc cttccacat 
        gcgacccag catctaatc gaaaccacc ggacctcca 
        gtagcttca aaaccacgg agactccct ccagacaaa 
        ctacaaata gccaaaaga gagttcgaa catatgatg 
        gcgctaggg atttgcaga ccctccaat agtccctgg 
        gcaagtccc ctccatatg gtccctaaa aaggacagc 
        aactggcgt ccggtaggt gactataga cgactcaac 
        gcggtcact aaagaggat cgttaccca attcctcat 
        ttacatgac ttcgctcac ctgcttgct ggtaagacc 
        attttttca accgttgac ctcgttaga gcttaccac 
        cagatcccg gtcgaagcg agctctatt ccgaaaacc 
        gccattacc acacctttt ggccttttc gaatttaca 
        agaatgcaa tttggtctt cgtaacgcc gctcaatcc 
        tttcaaaga ttcatccac gaagtcttg aatggtctc 
        gacttttgc ttcccctac ctggatgac attctcatc 
        gcctccact agcgaaagg gaacacact gaccacctt 
        cgcaaagtc tttgaaaga ttattaaag tatggcctt 
        actattaat cccgaaaaa tgttctttt ggaaattcc 
        aaagtgaat ttcctgggg tacgaagtt tcagcagat 
        ggcaccaag cccttaacc gaccgagtc aaggtcatt 
        ttgaactac ccgctacca aagacatcc aaagacgct 
        agaaggttc ctagccgct catcaagcc ccgatacac 
        aatctagtc aaaaacagc aaaaagaac gataaaaca 
        ccgctatct tggaccccc gaatcgaaa gcagcattc 
        gagctgtgt aaacacgac ctcgctcaa gcgacgctc 
        ttagtacac cctacaact actgatact atttcgctc 
        acagtcgac gcttctgac ttcgcaatg agggcagtg 
        ctcgaacaa aatcaagga ggtagttgg aaaccctta 
        agtttcttt tcgaaaaaa ttgacaccg gcccaacag 
        aaatatagc acgtatgat agggaactg ttggcaatt 
        tattccgcg gtcaaagcg ttccaacac tttcttgaa 
        ggccgtcat ttcgtaatt tacacagat cacaaaccc 
        cttgtgtat gctttcacg caaaaatct gacaaggca 
        actccgcga caagcacga catctcgac tacataagc 
        cagtttaca accgtcatc aaccacatt tctggcaaa 
        tccaatgta gttgccgac accttgtcc agaatcagc 
        gaaattgac acaccaact cctatcgat taccagaca 
        ctacgcgaa tcacaagac gtagatccc gaactgcaa 
        acacttctg gcacaaccc gattctaca gcactgaaa 
        ctgaaattg ttcaccgtc cctggttca gagaaacaa 
        atttattgc gacgattct aaccgcaga atccgtccg 
        ttcatcccc aaaacctca cggtatgaa cttttcaag 
        cactttcat ggtatggct caccccggt atcaaagcc 
        accacaaaa ctcctcact gacagattt gtctggcct 
        ggtattaac aaggacgtc agccgctgg actagagct 
        tgcataaac tgccagaga tccaaagtg cagaggcat 
        acacaacca ccgatcaca gaaattggt acttctgac 
        gaacgattc gcagttatc aacatagat ttgattgga 
        ccattacca ccttcaaac ggctttacc tattgccta 
        acttgtatc gaccgctac acttcttgg accgaagcc 
        attccgcta accgatatc acagccgaa tccgttgct 
        aaagccctg tattccggc tggatctcc cggtttggt 
        acgccgctg aaaattatc acggatcaa ggcaggcaa 
        ttcgaaagt tcgctattt gcttcactt tccacatta 
        atgggtttt cgccgcgcg aggtgcaca ccgtaccat 
        ccagccacc aacggcaaa attgaacgt tggcataga 
        acactaaag actgccatt aaagcccac gcttcacca 
        aactggacg gaacattta cccaccgtt ctcctcggg 
        ctgaggaca gtcatccgc gatgacacc ccagtctca 
        gcttcggaa atggtttat gggtcaacc attcggctc 
        cccggtgaa ttttttcaa gattcaacc aacaccatc 
        gaccctgca actttcgtg ggacaactc aaagcaaat 
        atcgcaaat gttcgacca gcgtcagct ccacatcat 
        gacaacagg cagatattt gttcctcaa aatcttgac 
        acctgcact cacgttttt gtccgaaga gacgcagtc 
        aggaaacct tttgaccca ccttatgat ggaccctgt 
        aaagttctc aatcgaacc gaaaagtat tttacggtt 
        gatctcaac aacaagtca accaacatt tctttgtcc 
        agactgaaa ccagctttt ctgctcaac gacaacccc 
        attcggcac gaccatact tatgccaat tgggctatt 
        acacccgaa aagactgat ttgaagaag actgtcaga 
        ttcaaatta taa
      mRNA sequence
      • >TC010212 RA
        gcgcatcga aaacaccac gcaacagtg cacgttaca 
        acatgcccg ataacccac cagatttca acgccgctg 
        tccaggtcg agatcgaga gggttactg ttaaaccgc 
        caccctttt ggaaaggtg atcccaagc tgtggttta 
        tccaacttg aagcccaat tcaacctcg gcaagataa 
        caagcgata caactaagt atcactacg tggtcagtg 
        ctatcgaca catcagttc ttcagcaaa ttgccgctt 
        tcgtgacca atcctcccg ctgtcaacc aatatgaag 
        gtatcaaac aacggctta tttctacat tttcggatt 
        ctcaggaac gccaattgc gtaaactac tgtcggaaa 
        tagacttag gcgacaaga aaccttcgc aattgctta 
        acgaaatga agcgtctgg gtgattcgg ctgtatccg 
        acgaattac tgaaaactt tgtggttac aaagattgc 
        cgacccacg tccaatccg tgctcgcaa ccagctccg 
        acccgattc agaaccttg cgcaaatgg cagacaaaa 
        tcgtggaga tacaacaca tgtcctcag tttgtacag 
        ttacgaccc caaccgaag atatgtctg cagctatcc 
        tgcaactca ctaaggaag tcgcagcat taaaggctg 
        cctacaata ctgaaatta agccgcgac gatcacgat 
        cacgaccac agacgccag accgttcaa gcagggact 
        cttgttggt accaccgaa catttggca ataaagcaa 
        agaagtgta tcactccct gcaacttca acaagtcgg 
        aaaactcca cgccgcgcc atcgacgcc tcaccataa 
        ccgatccaa ccaccggta tttcgtttt tgattgact 
        ctggttcgg atatttcaa tcatcccca agaaagcac 
        gacagtcaa cagtaagtg acctcgtac tttacgctg 
        ccaatggca ccgccattc aaacctacg gttacgaga 
        gacttacca ttactttgg gattacgac gtcgattaa 
        tttggaact tcatcatcg ctgatacca aacgagcaa 
        taattggcg ccgatttat tacattatt tcgatcttt 
        taatcgata ttagacggg ccagactaa ttgatagca 
        cgacaggaa tctccagcg caggcacat gcatttccg 
        cctcgcaca ctgcagtgt tgtcagttt caccgaacg 
        ctcggtatc gcgacctga tcagggaat ttccagctc 
        tgacaactt ttcacaatg tcgaaccaa tccttccac 
        atgcgaccc agcatctaa tcgaaacca ccggacctc 
        cagtagctt caaaaccac ggagactcc ctccagaca 
        aactacaaa tagccaaaa gagagttcg aacatatga 
        tggcgctag ggatttgca gaccctcca atagtccct 
        gggcaagtc ccctccata tggtcccta aaaaggaca 
        gcaactggc gtccggtag gtgactata gacgactca 
        acgcggtca ctaaagagg atcgttacc caattcctc 
        atttacatg acttcgctc acctgcttg ctggtaaga 
        ccatttttt caaccgttg acctcgtta gagcttacc 
        accagatcc cggtcgaag cgagctcta ttccgaaaa 
        ccgccatta ccacacctt ttggccttt tcgaattta 
        caagaatgc aatttggtc ttcgtaacg ccgctcaat 
        cctttcaaa gattcatcc acgaagtct tgaatggtc 
        tcgactttt gcttcccct acctggatg acattctca 
        tcgcctcca ctagcgaaa gggaacaca ctgaccacc 
        ttcgcaaag tctttgaaa gattattaa agtatggcc 
        ttactatta atcccgaaa aatgttctt ttggaaatt 
        ccaaagtga atttcctgg ggtacgaag tttcagcag 
        atggcacca agcccttaa ccgaccgag tcaaggtca 
        ttttgaact acccgctac caaagacat ccaaagacg 
        ctagaaggt tcctagccg ctcatcaag ccccgatac 
        acaatctag tcaaaaaca gcaaaaaga acgataaaa 
        caccgctat cttggaccc ccgaatcga aagcagcat 
        tcgagctgt gtaaacacg acctcgctc aagcgacgc 
        tcttagtac accctacaa ctactgata ctatttcgc 
        tcacagtcg acgcttctg acttcgcaa tgagggcag 
        tgctcgaac aaaatcaag gaggtagtt ggaaaccct 
        taagtttct tttcgaaaa aattgacac cggcccaac 
        agaaatata gcacgtatg atagggaac tgttggcaa 
        tttattccg cggtcaaag cgttccaac actttcttg 
        aaggccgtc atttcgtaa tttacacag atcacaaac 
        cccttgtgt atgctttca cgcaaaaat ctgacaagg 
        caactccgc gacaagcac gacatctcg actacataa 
        gccagttta caaccgtca tcaaccaca tttctggca 
        aatccaatg tagttgccg acaccttgt ccagaatca 
        gcgaaattg acacaccaa ctcctatcg attaccaga 
        cactacgcg aatcacaag acgtagatc ccgaactgc 
        aaacacttc tggcacaac ccgattcta cagcactga 
        aactgaaat tgttcaccg tccctggtt cagagaaac 
        aaatttatt gcgacgatt ctaaccgca gaatccgtc 
        cgttcatcc ccaaaacct cacggtatg aacttttca 
        agcactttc atggtatgg ctcaccccg gtatcaaag 
        ccaccacaa aactcctca ctgacagat ttgtctggc 
        ctggtatta acaaggacg tcagccgct ggactagag 
        cttgcataa actgccaga gatccaaag tgcagaggc 
        atacacaac caccgatca cagaaattg gtacttctg 
        acgaacgat tcgcagtta tcaacatag atttgattg 
        gaccattac caccttcaa acggcttta cctattgcc 
        taacttgta tcgaccgct acacttctt ggaccgaag 
        ccattccgc taaccgata tcacagccg aatccgttg 
        ctaaagccc tgtattccg gctggatct cccggtttg 
        gtacgccgc tgaaaatta tcacggatc aaggcaggc 
        aattcgaaa gttcgctat ttgcttcac tttccacat 
        taatgggtt ttcgccgcg cgaggtgca caccgtacc 
        atccagcca ccaacggca aaattgaac gttggcata 
        gaacactaa agactgcca ttaaagccc acgcttcac 
        caaactgga cggaacatt tacccaccg ttctcctcg 
        ggctgagga cagtcatcc gcgatgaca ccccagtct 
        cagcttcgg aaatggttt atgggtcaa ccattcggc 
        tccccggtg aattttttc aagattcaa ccaacacca 
        tcgaccctg caactttcg tgggacaac tcaaagcaa 
        atatcgcaa atgttcgac cagcgtcag ctccacatc 
        atgacaaca ggcagatat ttgttcctc aaaatcttg 
        acacctgca ctcacgttt ttgtccgaa gagacgcag 
        tcaggaaac cttttgacc caccttatg atggaccct 
        gtaaagttc tcaatcgaa ccgaaaagt attttacgg 
        ttgatctca acaacaagt caaccaaca tttctttgt 
        ccagactga aaccagctt ttctgctca acgacaacc 
        ccattcggc acgaccata cttatgcca attgggcta 
        ttacacccg aaaagactg atttgaaga agactgtca 
        gattcaaat tataaatag ttttttcta ttactcatc 
        gtggctcca ccactggca ggggagtac tgtggcggc 
        actggaacc acacaggcg attcacggt tgatcgtaa 
        ccgtagcca caacacttc ccatgacaa gtgacagcc 
        cgccagaac gacactagc gacaccacc tctcttggc 
        gtcgagcgt cactcggtc acacgtgca taattttag 
        ttttgtaac cgtttcaat ttgttgcgt taataaagg 
        agtttatgt aaa
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