Status
Please wait ...
Gene information
General information
TC010207 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 15834849..15840239
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010207
    iBeetle screen iB_04787

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010207 RA
      MPLKGSAPV CLKCETSES PLWTNAENL 
      GAICLDCVN EAKDNLKSE QEEENDDKS 
      EDSKSIKRK QRTTRSYKT RLNPFAIPK 
      IGTPKGRGR RGIFKKTPI KAPSAVATT 
      ITSESIFYK GSYIQVGDI VSMLDEDGD 
      IFYAQIKGL LTDQYCEKS AVITWLLPT 
      QESPPPEEG FDPATYIIG PEEDVPRKL 
      ECMEFVMHA PSDYYKSKN TPYPAVHAP 
      AQPGYIWTS LESIRRLCG NPLQNN
    CDS sequence
    • >TC010207 RA
      atgcccctt aaaggctct gctcccgtg tgcctcaag 
      tgcgaaaca agtgaatct ccactatgg accaacgcc 
      gaaaacctg ggggcaatc tgcttggac tgtgttaac 
      gaagcgaaa gataacctc aaatcggaa caagaagag 
      gaaaacgat gataaatcg gaagactca aaatccatc 
      aaacgcaaa cagcggact accaggtcg tataaaacg 
      aggttaaac cctttcgcg attcctaaa attgggacg 
      ccgaagggg agaggcaga agaggcatt tttaagaag 
      acgccaatt aaagcaccc tctgctgtg gccacaaca 
      atcacttct gaatcgatt ttttataaa ggaagttat 
      attcaagtg ggtgatatt gtttcaatg ctagatgag 
      gatggtgat attttttat gcccaaatt aaagggttg 
      ttaacggat cagtattgc gaaaaaagt gctgtgatt 
      acgtggctt ttgcctaca caagagagt ccaccacct 
      gaggaaggt tttgatcct gctacttac ataataggt 
      ccagaagaa gatgtccct aggaagtta gagtgcatg 
      gaatttgta atgcacgcc ccctctgat tattacaag 
      tcgaaaaat acgccctat ccggctgtg catgctcct 
      gcacaacct gggtatata tggacgtca ctggagtcg 
      ataagaaga ttgtgtgga aacccatta caaaacaac 
      tga
    mRNA sequence
    • >TC010207 RA
      tttactatt taatattga ctcaagata aaatccgtt 
      tttctaata gcaaaccta caaattccc ttcaaaaaa 
      atacatacg ctactggtg cttgcgtca cccttaatt 
      actcaagtg cttataatt taaataatc aacggatgt 
      ccgcgtatc catctcgaa gaattaagt tgattaatt 
      atttggaat gtgttgaca aaactgtcc cacgcttaa 
      aattaacaa cgatattgt acacgcacg tgtttcata 
      aatgggaga cccggcacc aaggcaggg caacagcag 
      aactagatt tttacattc ttgaaaaat ctctggttt 
      acactggct cgaacttac tccacaact aattgcgtc 
      agttataat ttacctatg ttaatagcc ctgggaatg 
      gaaatacat atatttaag tttaaggac tacaaatat 
      taattttct gatttctcg aaaagttgc tgaccttga 
      aattgacgt ttgtccaac aacataacc tcagtttca 
      aagtggtaa gatgcccct taaaggctc tgctcccgt 
      gtgcctcaa gtgcgaaac aagtgaatc tccactatg 
      gaccaacgc cgaaaacct gggggcaat ctgcttgga 
      ctgtgttaa cgaagcgaa agataacct caaatcgga 
      acaagaaga ggaaaacga tgataaatc ggaagactc 
      aaaatccat caaacgcaa acagcggac taccaggtc 
      gtataaaac gaggttaaa ccctttcgc gattcctaa 
      aattgggac gccgaaggg gagaggcag aagaggcat 
      ttttaagaa gacgccaat taaagcacc ctctgctgt 
      ggccacaac aatcacttc tgaatcgat tttttataa 
      aggaagtta tattcaagt gggtgatat tgtttcaat 
      gctagatga ggatggtga tatttttta tgcccaaat 
      taaagggtt gttaacgga tcagtattg cgaaaaaag 
      tgctgtgat tacgtggct tttgcctac acaagagag 
      tccaccacc tgaggaagg ttttgatcc tgctactta 
      cataatagg tccagaaga agatgtccc taggaagtt 
      agagtgcat ggaatttgt aatgcacgc cccctctga 
      ttattacaa gtcgaaaaa tacgcccta tccggctgt 
      gcatgctcc tgcacaacc tgggtatat atggacgtc 
      actggagtc gataagaag attgtgtgg aaacccatt 
      acaaaacaa ctgacaaaa attttgtgg atgttttgc 
      tttttgctt tatcacgta ttattttct gtgtattac 
      aaactacgt gattctttt actactttt tacatatat 
      tgtattaac acactgatt actcttgtt ttttctgtt 
      taaataaat ttatacaag taca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY