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Gene information
General information
TC010205 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 15936320..15938025
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010205
    iBeetle screen iB_04786

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010205 RA
      MSIYKSCRK LCPDFIVND FVTNTIKTI PIRAGYKTI 
      RVTLKNADL NDKNVIFCA TTSLSIVCR KGCLVDIVD 
      IVIKSTPTN KTLRQIIPL HEFFARETF FYNTILVDL 
      VEFYNGSVK IEVPTLIAA TLDDEDEAI ILQNAGWVP 
      KYEKWVGEL TQGHIFKVL QEFARLHAL SFAFSKQQP 
      EKDQQHRKQ LNTGYFSVF ENKQTVNIV RNNLNQVLK 
      FFNDDNVII KLRKFGDEI ETFFDNVLI EIPNKSVLV 
      HGDPTSNFL FYYEKNESG NIPSRAKLI DWHLVKFGS 
      PVLDLSLFF FTNCDDINN VDTFLKSYH VNLNIFLKE 
      LGCDIEEVF SQKNLEDHW TKYAKIGLA LAILTLKIL 
      TLEVNKRPN ANNEKLSIE EFIDAFQGP PKEDYVRKI 
      KDIVKYCVN NGLI
    CDS sequence
    • >TC010205 RA
      atgagtatc tacaagagt tgtcgaaaa ttgtgcccc 
      gatttcatt gtcaacgat ttcgtcacc aacactatt 
      aaaacaata ccaattcga gcgggttat aaaacgata 
      agagtgacc ttgaaaaac gctgattta aacgataaa 
      aatgtaatt ttctgtgca accacatcc ctgtctata 
      gtgtgtcga aaagggtgt cttgtcgat atcgtcgat 
      atagtgatt aaaagcacc ccaacgaat aaaaccctg 
      cgacagatt attcctctg catgaattt ttcgcgaga 
      gaaacattt ttttataac acgattctg gtggatttg 
      gtggaattt tacaatgga agtgttaaa attgaagtc 
      ccgacactc atcgctgcg accttagat gacgaagat 
      gaagcaata atcctgcaa aatgcagga tgggtgcca 
      aaatacgaa aagtgggtt ggagaatta acccaaggt 
      catattttc aaagtcttg caggaattt gccagatta 
      cacgcgctg agtttcgct ttcagtaaa caacaaccg 
      gaaaaggat cagcagcat agaaagcaa ctcaacacg 
      ggctatttt agtgttttc gaaaataaa caaactgtt 
      aacattgta cgaaacaat ttaaaccaa gtgctaaaa 
      tttttcaac gacgataat gttattata aagttgaga 
      aaattcgga gatgaaatt gaaacattc ttcgacaac 
      gttttaatt gaaattcca aacaaaagt gttttagta 
      catggagat cccaccagc aattttctg ttttattac 
      gagaaaaat gaaagtggt aacatccca tcaagggcc 
      aagttaatt gattggcat ttagttaaa tttggatca 
      ccagtgttg gatctctcg ctgtttttc tttacgaat 
      tgtgatgat attaataat gtggatact tttctcaag 
      agttatcac gtcaacttg aatattttt ttgaaagag 
      ttgggttgt gacattgaa gaagttttc tcacagaaa 
      aatttggaa gatcattgg actaaatat gcaaaaatt 
      ggtcttgct cttgcaatt ttaacattg aagattctt 
      acattggaa gttaataaa aggccgaat gcaaataat 
      gaaaaatta tcaattgaa gaatttatt gacgctttt 
      caaggcccc ccaaaggag gattatgtg agaaaaatt 
      aaggatatt gttaagtac tgtgtgaat aatggactg 
      atttga
    mRNA sequence
    • >TC010205 RA
      ttcattaat tggcggcaa attcaaaca aaacgcttc 
      acatctcaa ttacataac aaaaataac taacccaaa 
      taaaagtgc gcgttaata aaccacgcc cgagttatt 
      cgctttcag caatgagta tctacaaga gttgtcgaa 
      aattgtgcc ccgatttca ttgtcaacg atttcgtca 
      ccaacacta ttaaaacaa taccaattc gagcgggtt 
      ataaaacga taagagtga ccttgaaaa acgctgatt 
      taaacgata aaaatgtaa ttttctgtg caaccacat 
      ccctgtcta tagtgtgtc gaaaagggt gtcttgtcg 
      atatcgtcg atatagtga ttaaaagca ccccaacga 
      ataaaaccc tgcgacaga ttattcctc tgcatgaat 
      ttttcgcga gagaaacat ttttttata acacgattc 
      tggtggatt tggtggaat tttacaatg gaagtgtta 
      aaattgaag tcccgacac tcatcgctg cgaccttag 
      atgacgaag atgaagcaa taatcctgc aaaatgcag 
      gatgggtgc caaaatacg aaaagtggg ttggagaat 
      taacccaag gtcatattt tcaaagtct tgcaggaat 
      ttgccagat tacacgcgc tgagtttcg ctttcagta 
      aacaacaac cggaaaagg atcagcagc atagaaagc 
      aactcaaca cgggctatt ttagtgttt tcgaaaata 
      aacaaactg ttaacattg tacgaaaca atttaaacc 
      aagtgctaa aatttttca acgacgata atgttatta 
      taaagttga gaaaattcg gagatgaaa ttgaaacat 
      tcttcgaca acgttttaa ttgaaattc caaacaaaa 
      gtgttttag tacatggag atcccacca gcaattttc 
      tgttttatt acgagaaaa atgaaagtg gtaacatcc 
      catcaaggg ccaagttaa ttgattggc atttagtta 
      aatttggat caccagtgt tggatctct cgctgtttt 
      tctttacga attgtgatg atattaata atgtggata 
      cttttctca agagttatc acgtcaact tgaatattt 
      ttttgaaag agttgggtt gtgacattg aagaagttt 
      tctcacaga aaaatttgg aagatcatt ggactaaat 
      atgcaaaaa ttggtcttg ctcttgcaa ttttaacat 
      tgaagattc ttacattgg aagttaata aaaggccga 
      atgcaaata atgaaaaat tatcaattg aagaattta 
      ttgacgctt ttcaaggcc ccccaaagg aggattatg 
      tgagaaaaa ttaaggata ttgttaagt actgtgtga 
      ataatggac tgatttgaa caagtggtt gaagcttga 
      tgggactta ctaaaaatt gtttttttg cttaacagg 
      aaatagaaa atgcgagca ctgctactt cgcagaaga 
      aaacgatta caagaattt aactaactt tttcctgct 
      gccgataac gcaaataag tcttaccac catagcaac 
      atagcccgt aagtcgacg ataacattg gtttctatg 
      acagaattt aatatttgt acaaaagtc cttggaaaa 
      taataataa tataataat aaataatga cactaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY