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Gene information
General information
TC010201 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 13115318..13116771
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC010201
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04784

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): less than 30%
    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    No observeable cuticle phenotype found.
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010201 RA
      MATRTLPKN FYSNPAFSQ QSEFYNGKP LSQIVQNVR 
QSPVGAQTQ PQYRENEEY YDEEFYEEM IVDDAGIIK 
QKKVLVVSS PTKDQKSRY EYIPMQEQN RYIICQKQP 
PQVHYAEEF VQGRPNHRY EYIPEESPP KSVTRNRYE 
YIQFEPPSP RKPSNPIAT QKLHELLST PKKPPVMSP 
QAHRRVPPS PQRDPFITP RKSQTRQKL NYALGTRQA 
QDKRHTAIV APMCSSPIQ SVYSETTFS KTDSWMNLG 
PRKPTQVTL AVAAVMMML CGGVTSGLC FYMVSIMGR 
LYFLDFGIV AGFTCLVLG FLGFRTRNC YWLPNRNYI 
SGYIVLSVF SLLMCAGLL VLLVLQPKP GTPLADMTS 
GAVCGISVL SLVLASAGV MTSYCCKYP PPDNRVEHS 
VPGFTV
    CDS sequence
    • >TC010201 RA
      atggccacg cgaaccctt ccgaaaaac ttctactcg 
aacccggcc ttcagccaa caaagtgaa ttttacaac 
ggaaaaccg ctctcccaa atcgtccaa aacgtgcgc 
cagtcgccc gtcggggcg caaacccag ccccagtac 
cgcgaaaac gaagaatac tacgatgaa gaattctac 
gaagaaatg atcgtcgac gatgctgga atcatcaag 
cagaagaaa gtcctagtc gtatcctcg cccaccaag 
gaccaaaag tcccggtac gagtacatc cccatgcag 
gagcaaaac cgctacatc atctgccaa aaacaaccg 
ccgcaagtg cactacgcc gaagagttc gtccaaggc 
aggcccaac caccgctac gagtacatc ccggaggag 
tccccgcca aaatccgtt acccggaac cgttacgaa 
tacatccag ttcgaaccg ccaagtccc cggaaaccc 
tccaacccc atcgcgacc caaaagctc cacgagctc 
ctctcaacc cccaaaaag ccccccgta atgtccccc 
caagcccac cgaagagtc cctccatcc ccccagcgg 
gaccccttc ataacgccg cgcaaaagc caaacccgc 
caaaaacta aactacgcc ctcggcacg cgccaagcg 
caggacaag cgccacacg gccatcgtg gcccccatg 
tgctccagc cccatccag agcgtctac agcgagacc 
acgttctcc aaaaccgac tcttggatg aacttaggg 
ccgaggaaa ccgacccaa gtgacgctg gccgtggcc 
gcggtgatg atgatgctc tgcgggggg gtcacctcg 
gggctgtgc ttctacatg gtgtccatc atgggccgg 
ctgtatttc ctggatttc gggattgtg gcagggttc 
acttgtctg gtgttgggg tttttgggg ttccggacg 
cggaattgt tactggctg cccaaccgg aattacatt 
tccgggtat atcgttttg agtgtgttt agtcttctg 
atgtgtgcc gggcttctg gtgctgctt gtgctgcag 
cccaagccc ggcacgcct ctcgcggat atgacttcc 
ggtgccgtt tgcgggatt tccgttttg tctctagtg 
ttggcatct gcaggagtc atgacgagt tattgttgc 
aaatatccg ccacctgac aacagggtg gagcacagc 
gtcccagga ttcacggtt taa
    mRNA sequence
    • >TC010201 RA
      ccaggcgac cagtcatca ccgggccgt gccccaaac 
gactacaaa tgacggttg cgagcgcat ttcgacttt 
aaaatggcc acgcgaacc cttccgaaa aacttctac 
tcgaacccg gccttcagc caacaaagt gaattttac 
aacggaaaa ccgctctcc caaatcgtc caaaacgtg 
cgccagtcg cccgtcggg gcgcaaacc cagccccag 
taccgcgaa aacgaagaa tactacgat gaagaattc 
tacgaagaa atgatcgtc gacgatgct ggaatcatc 
aagcagaag aaagtccta gtcgtatcc tcgcccacc 
aaggaccaa aagtcccgg tacgagtac atccccatg 
caggagcaa aaccgctac atcatctgc caaaaacaa 
ccgccgcaa gtgcactac gccgaagag ttcgtccaa 
ggcaggccc aaccaccgc tacgagtac atcccggag 
gagtccccg ccaaaatcc gttacccgg aaccgttac 
gaatacatc cagttcgaa ccgccaagt ccccggaaa 
ccctccaac cccatcgcg acccaaaag ctccacgag 
ctcctctca acccccaaa aagcccccc gtaatgtcc 
ccccaagcc caccgaaga gtccctcca tccccccag 
cgggacccc ttcataacg ccgcgcaaa agccaaacc 
cgccaaaaa ctaaactac gccctcggc acgcgccaa 
gcgcaggac aagcgccac acggccatc gtggccccc 
atgtgctcc agccccatc cagagcgtc tacagcgag 
accacgttc tccaaaacc gactcttgg atgaactta 
gggccgagg aaaccgacc caagtgacg ctggccgtg 
gccgcggtg atgatgatg ctctgcggg ggggtcacc 
tcggggctg tgcttctac atggtgtcc atcatgggc 
cggctgtat ttcctggat ttcgggatt gtggcaggg 
ttcacttgt ctggtgttg gggtttttg gggttccgg 
acgcggaat tgttactgg ctgcccaac cggaattac 
atttccggg tatatcgtt ttgagtgtg tttagtctt 
ctgatgtgt gccgggctt ctggtgctg cttgtgctg 
cagcccaag cccggcacg cctctcgcg gatatgact 
tccggtgcc gtttgcggg atttccgtt ttgtctcta 
gtgttggca tctgcagga gtcatgacg agttattgt 
tgcaaatat ccgccacct gacaacagg gtggagcac 
agcgtccca ggattcacg gtttaaaac cacttattt 
attttatag taatataat tattatggt ttatattat 
ttaatgact ggaaattgt aaatattat ttattgtag 
gtattgatt gtttttatc taataaacg gttttataa 
caa

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY