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Gene information
General information
TC010201 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 13115318..13116771
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010201
    iBeetle screen iB_04784

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): less than 30%
    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    No observeable cuticle phenotype found.

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010201 RA
      MATRTLPKN FYSNPAFSQ QSEFYNGKP LSQIVQNVR 
      QSPVGAQTQ PQYRENEEY YDEEFYEEM IVDDAGIIK 
      QKKVLVVSS PTKDQKSRY EYIPMQEQN RYIICQKQP 
      PQVHYAEEF VQGRPNHRY EYIPEESPP KSVTRNRYE 
      YIQFEPPSP RKPSNPIAT QKLHELLST PKKPPVMSP 
      QAHRRVPPS PQRDPFITP RKSQTRQKL NYALGTRQA 
      QDKRHTAIV APMCSSPIQ SVYSETTFS KTDSWMNLG 
      PRKPTQVTL AVAAVMMML CGGVTSGLC FYMVSIMGR 
      LYFLDFGIV AGFTCLVLG FLGFRTRNC YWLPNRNYI 
      SGYIVLSVF SLLMCAGLL VLLVLQPKP GTPLADMTS 
      GAVCGISVL SLVLASAGV MTSYCCKYP PPDNRVEHS 
      VPGFTV
    CDS sequence
    • >TC010201 RA
      atggccacg cgaaccctt ccgaaaaac ttctactcg 
      aacccggcc ttcagccaa caaagtgaa ttttacaac 
      ggaaaaccg ctctcccaa atcgtccaa aacgtgcgc 
      cagtcgccc gtcggggcg caaacccag ccccagtac 
      cgcgaaaac gaagaatac tacgatgaa gaattctac 
      gaagaaatg atcgtcgac gatgctgga atcatcaag 
      cagaagaaa gtcctagtc gtatcctcg cccaccaag 
      gaccaaaag tcccggtac gagtacatc cccatgcag 
      gagcaaaac cgctacatc atctgccaa aaacaaccg 
      ccgcaagtg cactacgcc gaagagttc gtccaaggc 
      aggcccaac caccgctac gagtacatc ccggaggag 
      tccccgcca aaatccgtt acccggaac cgttacgaa 
      tacatccag ttcgaaccg ccaagtccc cggaaaccc 
      tccaacccc atcgcgacc caaaagctc cacgagctc 
      ctctcaacc cccaaaaag ccccccgta atgtccccc 
      caagcccac cgaagagtc cctccatcc ccccagcgg 
      gaccccttc ataacgccg cgcaaaagc caaacccgc 
      caaaaacta aactacgcc ctcggcacg cgccaagcg 
      caggacaag cgccacacg gccatcgtg gcccccatg 
      tgctccagc cccatccag agcgtctac agcgagacc 
      acgttctcc aaaaccgac tcttggatg aacttaggg 
      ccgaggaaa ccgacccaa gtgacgctg gccgtggcc 
      gcggtgatg atgatgctc tgcgggggg gtcacctcg 
      gggctgtgc ttctacatg gtgtccatc atgggccgg 
      ctgtatttc ctggatttc gggattgtg gcagggttc 
      acttgtctg gtgttgggg tttttgggg ttccggacg 
      cggaattgt tactggctg cccaaccgg aattacatt 
      tccgggtat atcgttttg agtgtgttt agtcttctg 
      atgtgtgcc gggcttctg gtgctgctt gtgctgcag 
      cccaagccc ggcacgcct ctcgcggat atgacttcc 
      ggtgccgtt tgcgggatt tccgttttg tctctagtg 
      ttggcatct gcaggagtc atgacgagt tattgttgc 
      aaatatccg ccacctgac aacagggtg gagcacagc 
      gtcccagga ttcacggtt taa
    mRNA sequence
    • >TC010201 RA
      ccaggcgac cagtcatca ccgggccgt gccccaaac 
      gactacaaa tgacggttg cgagcgcat ttcgacttt 
      aaaatggcc acgcgaacc cttccgaaa aacttctac 
      tcgaacccg gccttcagc caacaaagt gaattttac 
      aacggaaaa ccgctctcc caaatcgtc caaaacgtg 
      cgccagtcg cccgtcggg gcgcaaacc cagccccag 
      taccgcgaa aacgaagaa tactacgat gaagaattc 
      tacgaagaa atgatcgtc gacgatgct ggaatcatc 
      aagcagaag aaagtccta gtcgtatcc tcgcccacc 
      aaggaccaa aagtcccgg tacgagtac atccccatg 
      caggagcaa aaccgctac atcatctgc caaaaacaa 
      ccgccgcaa gtgcactac gccgaagag ttcgtccaa 
      ggcaggccc aaccaccgc tacgagtac atcccggag 
      gagtccccg ccaaaatcc gttacccgg aaccgttac 
      gaatacatc cagttcgaa ccgccaagt ccccggaaa 
      ccctccaac cccatcgcg acccaaaag ctccacgag 
      ctcctctca acccccaaa aagcccccc gtaatgtcc 
      ccccaagcc caccgaaga gtccctcca tccccccag 
      cgggacccc ttcataacg ccgcgcaaa agccaaacc 
      cgccaaaaa ctaaactac gccctcggc acgcgccaa 
      gcgcaggac aagcgccac acggccatc gtggccccc 
      atgtgctcc agccccatc cagagcgtc tacagcgag 
      accacgttc tccaaaacc gactcttgg atgaactta 
      gggccgagg aaaccgacc caagtgacg ctggccgtg 
      gccgcggtg atgatgatg ctctgcggg ggggtcacc 
      tcggggctg tgcttctac atggtgtcc atcatgggc 
      cggctgtat ttcctggat ttcgggatt gtggcaggg 
      ttcacttgt ctggtgttg gggtttttg gggttccgg 
      acgcggaat tgttactgg ctgcccaac cggaattac 
      atttccggg tatatcgtt ttgagtgtg tttagtctt 
      ctgatgtgt gccgggctt ctggtgctg cttgtgctg 
      cagcccaag cccggcacg cctctcgcg gatatgact 
      tccggtgcc gtttgcggg atttccgtt ttgtctcta 
      gtgttggca tctgcagga gtcatgacg agttattgt 
      tgcaaatat ccgccacct gacaacagg gtggagcac 
      agcgtccca ggattcacg gtttaaaac cacttattt 
      attttatag taatataat tattatggt ttatattat 
      ttaatgact ggaaattgt aaatattat ttattgtag 
      gtattgatt gtttttatc taataaacg gttttataa 
      caa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY