Status
Please wait ...
Gene information
General information
TC033836
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010198,TC010197
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 13132542..13138771
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033836
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04783

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033836 RA
      MSKKLMRTI IDDLSQVLQ PETNLPHAQ DTGLSLIIV 
      VASTLRLSV EYVKIYYRN DPVKWRPLL VLGVGLALF 
      HTCQCDETT EVEYSVSSS TSVEQAKNS TDGFPEPAA 
      LVDQNANHT REALTRDAS DHESVVFIG DTAGYVPIP 
      NLRTRDFPS APDDVDWHH PVESWDREY RRFRFNTTR 
      VQHIEAECQ DDYMKIRIG FNGTFTGLV YSAGYAYDP 
      DCMYINGTG RDYYEFYIQ LNRCGTLGK NTHNEDNRK 
      TPTKNFMWN TVTVQYNPL IEEEYDEHF KVTCEYGYD 
      FWKTVTFPF LDVEVATGN PVVFTLTPP ECYMEIRYG 
      YGTGGNRVT GPVRVGDPL TLIIYMRSK YDGFDIVVN 
      DCFAHNGGH KKIQLIDEY GCPVDDKLI SRFRGSWSE 
      TGVYETQVF AYMKTFRFT GSPALYIEC DVRMCHGRC 
      PSQPCHWRN VKNVRKRSV DSSPQTTTL PANIAPLSE 
      NVNLFQSLR VLQEGESEE NRNVTLTRY EVDPNSVCL 
      KTGWFSALL ALGGGAMCL LGGALLATC TRMRRVAID 
      KVLAGSQFN GYMNHKGSI N
    CDS sequence
    • >TC033836 RA
      atgagcaaa aagctcatg cgaacgatc atcgatgac 
      ttgagccag gttttgcaa cctgaaaca aatctgcct 
      cacgctcag gatacagga ctgtccctc atcatcgtg 
      gttgcgtca actctacgt ttgagtgta gagtatgtg 
      aagatatac tacagaaat gatcctgtg aagtggcga 
      ccccttctc gtcttaggc gtagggctg gcgcttttc 
      cacacatgt caatgcgac gaaaccacc gaagtagag 
      tatagtgtt agcagtagt actagcgtc gaacaagcg 
      aaaaacagc acggacggc tttccggag cccgcggct 
      ctggtggac caaaacgcc aaccacacc agagaggcc 
      ctcacgcgc gatgccagc gaccacgaa tccgtggtg 
      ttcattggc gacaccgcc ggatacgtc ccgatcccc 
      aatttgcgc acccgggac ttcccctcg gcgcccgac 
      gacgtggac tggcaccat ccggtcgaa agctgggac 
      agggagtat cggcgtttc cggttcaac accacgcgg 
      gtccaacat atcgaagcc gaatgccag gacgactac 
      atgaaaatc aggataggg ttcaatggg accttcacg 
      gggctggtc tactccgca ggctacgct tacgacccc 
      gattgcatg tacataaac ggcacaggc cgggattat 
      tacgaattt tacattcaa ttgaaccgt tgcggcact 
      ttggggaaa aacacgcat aatgaagat aatcggaag 
      actcctacg aaaaatttc atgtggaac accgtgacg 
      gtgcaatac aacccgctg atcgaggaa gaatacgac 
      gaacacttc aaagtgacg tgcgaatac ggctacgat 
      ttttggaag acagttaca tttccgttc ctagatgtt 
      gaagtggcg actggaaat ccggtggtg ttcactctc 
      acacctccg gaatgctac atggaaatc cggtacggt 
      tacggaacc ggcggtaat agagtcacc ggaccggtc 
      cgggtgggc gatcctttg accctcatt atttacatg 
      cggagcaaa tacgacggg tttgatatc gtggtaaac 
      gactgcttc gcacataac gggggccac aagaaaatc 
      caactcatt gacgagtac ggatgtccg gtcgacgat 
      aagctgatt tcgcgattt cggggctcg tggtcagag 
      accggagtt tacgaaacg caggttttc gcttacatg 
      aagacgttc cggtttacc ggatccccg gcgctttac 
      atcgaatgt gacgtcaga atgtgccac ggaaggtgt 
      ccgagccaa ccgtgccac tggcgaaac gtgaaaaac 
      gtcaggaaa cgttcggtg gactcgagc ccccagacc 
      accactttg cccgcaaac atcgccccc ttgtcggaa 
      aatgtgaac ctgttccag tctttgcgg gtgctacag 
      gagggcgaa tcggaagaa aaccgaaac gtcacgctg 
      actcgttac gaagtggac ccgaatagt gtttgtttg 
      aaaaccggc tggttctcc gcactccta gcactgggc 
      ggcggcgcg atgtgtctc ctggggggc gccctttta 
      gccacttgc acccgaatg agaagagtt gccatagat 
      aaagtatta gcaggttcc cagtttaac ggttacatg 
      aaccacaag ggcagtatt aattaa
    mRNA sequence
    • >TC033836 RA
      gggagtaaa taagcttta aattgatta aaaacaacc 
      actcagact gtcctgcat ccatgacat gagcaaaaa 
      gctcatgcg aacgatcat cgatgactt gagccaggt 
      tttgcaacc tgaaacaaa tctgcctca cgctcagga 
      tacaggact gtccctcat catcgtggt tgcgtcaac 
      tctacgttt gagtgtaga gtatgtgaa gatatacta 
      cagaaatga tcctgtgaa gtggcgacc ccttctcgt 
      cttaggcgt agggctggc gcttttcca cacatgtca 
      atgcgacga aaccaccga agtagagta tagtgttag 
      cagtagtac tagcgtcga acaagcgaa aaacagcac 
      ggacggctt tccggagcc cgcggctct ggtggacca 
      aaacgccaa ccacaccag agaggccct cacgcgcga 
      tgccagcga ccacgaatc cgtggtgtt cattggcga 
      caccgccgg atacgtccc gatccccaa tttgcgcac 
      ccgggactt cccctcggc gcccgacga cgtggactg 
      gcaccatcc ggtcgaaag ctgggacag ggagtatcg 
      gcgtttccg gttcaacac cacgcgggt ccaacatat 
      cgaagccga atgccagga cgactacat gaaaatcag 
      gatagggtt caatgggac cttcacggg gctggtcta 
      ctccgcagg ctacgctta cgaccccga ttgcatgta 
      cataaacgg cacaggccg ggattatta cgaatttta 
      cattcaatt gaaccgttg cggcacttt ggggaaaaa 
      cacgcataa tgaagataa tcggaagac tcctacgaa 
      aaatttcat gtggaacac cgtgacggt gcaatacaa 
      cccgctgat cgaggaaga atacgacga acacttcaa 
      agtgacgtg cgaatacgg ctacgattt ttggaagac 
      agttacatt tccgttcct agatgttga agtggcgac 
      tggaaatcc ggtggtgtt cactctcac acctccgga 
      atgctacat ggaaatccg gtacggtta cggaaccgg 
      cggtaatag agtcaccgg accggtccg ggtgggcga 
      tcctttgac cctcattat ttacatgcg gagcaaata 
      cgacgggtt tgatatcgt ggtaaacga ctgcttcgc 
      acataacgg gggccacaa gaaaatcca actcattga 
      cgagtacgg atgtccggt cgacgataa gctgatttc 
      gcgatttcg gggctcgtg gtcagagac cggagttta 
      cgaaacgca ggttttcgc ttacatgaa gacgttccg 
      gtttaccgg atccccggc gctttacat cgaatgtga 
      cgtcagaat gtgccacgg aaggtgtcc gagccaacc 
      gtgccactg gcgaaacgt gaaaaacgt caggaaacg 
      ttcggtgga ctcgagccc ccagaccac cactttgcc 
      cgcaaacat cgccccctt gtcggaaaa tgtgaacct 
      gttccagtc tttgcgggt gctacagga gggcgaatc 
      ggaagaaaa ccgaaacgt cacgctgac tcgttacga 
      agtggaccc gaatagtgt ttgtttgaa aaccggctg 
      gttctccgc actcctagc actgggcgg cggcgcgat 
      gtgtctcct ggggggcgc ccttttagc cacttgcac 
      ccgaatgag aagagttgc catagataa agtattagc 
      aggttccca gtttaacgg ttacatgaa ccacaaggg 
      cagtattaa ttaaatcaa atagactaa ttaacagtg 
      caattatta aaaaagctt tatttttat aacgcattt 
      acgaacagt gagtgatta tgaaacgta ttattaaat 
      taagtaact aattaataa aaacactca ttaatttat 
      tttacctaa ggattagca aacaagcac tagaataat 
      gtatgttag tgaagtttc tcttagttt tagcaaaaa 
      taaatacac atccatgga ggattagtt taaaaaatg 
      cgtaaactt cttcttctg catgtgtat tattaagtg 
      tgcgttaca tcgccagac atgtatcga acacttcca 
      aactgtaca taaacactt agttttagt ccgcaataa 
      acttttgga aatttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY