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Gene information
General information
TC010196 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 13151554..13155861
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010196
    iBeetle screen iB_04782

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010196 RA
      MMTPLRLCA IYCLFSISS TSSAFPPSL RYPIPISVN 
      KITAANLTC DVRSFNATV TLQHPFKGL VFAKDFSHE 
      CNALGTLSN SVTINLPTA GCGIRLSSR ANEKGEKEL 
      VYSVVLVAQ QDRHLRQIA DQEKHLECV VKDDAFLIE 
      SQPVVDVLK NDLLERRGK SQRSGRMKK DQDLEDEIR 
      ETFSVARVW MEITPEGDE ANGGTLKVG EAAVLTIKS 
      TLPEGIGWK VVECSAHDG LGDSSQKLL DDYGCPTDE 
      LLFPVFRMG PVQVISVMK HQEAVSRFA AFKFPDRDR 
      LHLSCYLEL CRGTCPEVD CSDKNSTDG EVLDRLKVF 
      NSVEVLAPA IDDLRKSER LQLSEPTTS PFSSLPGDR 
      KFCVSPDKV AITFCILGV IFLCAVVVA ACALLRSRK 
      NGDESPFYP RSLFSSSTS GSAYGSKLL LHESPCMGQ 
      SPSRRMPYG RIL
    CDS sequence
    • >TC010196 RA
      atgatgaca ccgctccgg ttatgtgct atctactgc 
      ctcttttcg atttcttca accagctca gcgtttcct 
      ccaagcctg aggtaccca ataccaatt tcagttaac 
      aaaatcacc gccgctaat ttaacctgc gatgtgcga 
      agtttcaac gccactgtt accctccag cacccgttc 
      aaaggactt gtttttgcc aaagatttt tcgcacgaa 
      tgcaatgct ctcggaact ttatcaaac tctgtcaca 
      ataaacctc ccaactgcc ggttgtggg attagactg 
      agttcccga gcgaacgaa aaaggggag aaagaactg 
      gtctacagt gtggtcctg gtggcccaa caagaccgc 
      catttgcgc caaatagcc gaccaggaa aagcacctc 
      gagtgtgtg gtcaaagac gacgcgttc ctcatcgaa 
      agccaaccc gtggttgac gtcctaaag aacgatttg 
      ctcgaacga agaggcaaa agccagcga agtggtcgc 
      atgaaaaaa gaccaggat ctagaagac gagattcgc 
      gaaactttt agcgttgcc cgtgtttgg atggaaatc 
      actccggaa ggagacgaa gcaaatggt ggtactttg 
      aaagtcggc gaagcggca gttttgacc atcaagtcg 
      acgcttcct gagggaatc ggctggaag gtggttgag 
      tgcagcgcc catgatggt ctcggtgac tcctcgcaa 
      aaactgcta gacgattat gggtgtccc acggatgag 
      ctcctgttc ccagttttt cgaatgggt ccagtgcaa 
      gtcatctca gtcatgaaa caccaagaa gcggtctcg 
      aggttcgca gcgttcaag tttccggac agagaccga 
      ctccatttg tcgtgttat ctggaattg tgccgtggg 
      acttgtccc gaggtcgat tgctccgac aagaattca 
      actgatggc gaggtttta gaccggctg aaagtcttc 
      aatagtgtg gaggtgcta gcaccggct attgatgac 
      ttgaggaag agcgagagg ttgcagctc agcgaaccc 
      acaacatcc cctttttct agccttcca ggcgatcgg 
      aagttctgc gtctctcca gacaaagtc gccatcacg 
      ttttgtatc ttaggtgtc atattcctg tgtgctgtt 
      gtcgtcgca gcttgtgct ctgctcaga agtcgcaaa 
      aacggcgac gaaagtccg ttctatccc agaagtctg 
      ttttcgagc agcacaagc ggatcggcc tacggcagt 
      aagctgctc ctgcacgaa agcccgtgt atggggcag 
      tctccttcg agaaggatg ccttatggg cggatatta 
      taa
    mRNA sequence
    • >TC010196 RA
      actgcaaag tcccgtaaa aactgtatt tgtcaggta 
      acgctacgt acgttcgct cacctggct cacagttta 
      atcccaaca cggtttaag aatcgcaac actaaacga 
      aaatgcacg atattccta aatacacaa gtgtccagt 
      gagaaaact tcgaatttt tggacccac agatcagtt 
      gaatgatga caccgctcc ggttatgtg ctatctact 
      gcctctttt cgatttctt caaccagct cagcgtttc 
      ctccaagcc tgaggtacc caataccaa tttcagtta 
      acaaaatca ccgccgcta atttaacct gcgatgtgc 
      gaagtttca acgccactg ttaccctcc agcacccgt 
      tcaaaggac ttgtttttg ccaaagatt tttcgcacg 
      aatgcaatg ctctcggaa ctttatcaa actctgtca 
      caataaacc tcccaactg ccggttgtg ggattagac 
      tgagttccc gagcgaacg aaaaagggg agaaagaac 
      tggtctaca gtgtggtcc tggtggccc aacaagacc 
      gccatttgc gccaaatag ccgaccagg aaaagcacc 
      tcgagtgtg tggtcaaag acgacgcgt tcctcatcg 
      aaagccaac ccgtggttg acgtcctaa agaacgatt 
      tgctcgaac gaagaggca aaagccagc gaagtggtc 
      gcatgaaaa aagaccagg atctagaag acgagattc 
      gcgaaactt ttagcgttg cccgtgttt ggatggaaa 
      tcactccgg aaggagacg aagcaaatg gtggtactt 
      tgaaagtcg gcgaagcgg cagttttga ccatcaagt 
      cgacgcttc ctgagggaa tcggctgga aggtggttg 
      agtgcagcg cccatgatg gtctcggtg actcctcgc 
      aaaaactgc tagacgatt atgggtgtc ccacggatg 
      agctcctgt tcccagttt ttcgaatgg gtccagtgc 
      aagtcatct cagtcatga aacaccaag aagcggtct 
      cgaggttcg cagcgttca agtttccgg acagagacc 
      gactccatt tgtcgtgtt atctggaat tgtgccgtg 
      ggacttgtc ccgaggtcg attgctccg acaagaatt 
      caactgatg gcgaggttt tagaccggc tgaaagtct 
      tcaatagtg tggaggtgc tagcaccgg ctattgatg 
      acttgagga agagcgaga ggttgcagc tcagcgaac 
      ccacaacat ccccttttt ctagccttc caggcgatc 
      ggaagttct gcgtctctc cagacaaag tcgccatca 
      cgttttgta tcttaggtg tcatattcc tgtgtgctg 
      ttgtcgtcg cagcttgtg ctctgctca gaagtcgca 
      aaaacggcg acgaaagtc cgttctatc ccagaagtc 
      tgttttcga gcagcacaa gcggatcgg cctacggca 
      gtaagctgc tcctgcacg aaagcccgt gtatggggc 
      agtctcctt cgagaagga tgccttatg ggcggatat 
      tataacaca actttgtaa ataaatgtt tttggggcg 
      gttcaaggt gggtttggg gcttcgttt tgtctgggc 
      actcagccg ttctaaaac tggaacgcg aattcggac 
      gaagtactt ctattttta aacagtatt acatgaata 
      attactcgc tggaaggtt taatgtctt tattgtcga 
      aaattatta atccgcgag tagtcaaat tatgtttaa 
      ttataattg ttgtgatac gtcgaaaat aaaataaac 
      tatgtttta attc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY