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Gene information
General information
TC010181 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 13469505..13471211
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010181
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04779

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 10 (16 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010181 RA
      MNYNANTGN RPAHSRKVK RVSDVNAMG YTPYDPTQG 
      MPNNLYPNL GSESPAPNP YQQNFASPS FSSTQNYGV 
      PPNNPQMYG FNPASPYPN AQPPPNPQF GGVFGQPMV 
      QDMALQYGQ QLANTGKSM IKQEVEKYV PVNSLKYYF 
      AVDTKYVLS KLMLLFFPF THKDWSVKY EQDGPVQPR 
      FEINAPDLY IPTMAYVTY VLVAGMVLG MQQKFTPEQ 
      IGILASSAL AWFVVELAV YSCTLYIAN IKTTLRTFD 
      LLAFSGYKF VGIIVSILV SLIGAKTAY YCCLIYVNL 
      ALAFFLVRT LKAQVLVES NAQPTSYYG DVAPPTGNK 
      RRLYFLLFV AAVQPVLSW WLSFHLIGS PSPEKPVTE
    CDS sequence
    • >TC010181 RA
      atgaattac aacgcgaat acagggaac cggccagcc 
      catagccgg aaagttaaa agggttagt gacgtaaat 
      gccatgggg tacacaccg tacgatccc acgcaaggg 
      atgccaaac aatttgtac cctaatttg gggtccgaa 
      agccctgcc ccaaaccca tatcaacaa aattttgca 
      agcccgtcg tttagttca acccaaaat tatggggtg 
      ccaccaaac aacccccaa atgtatggg tttaatccc 
      gcttcaccc tatccaaac gcacagcca cccccgaat 
      ccgcagttc gggggcgtt tttggacaa ccaatggtc 
      caagacatg gccttgcag tatggacaa cagttggca 
      aatacggga aagtcgatg ataaaacaa gaagtggaa 
      aagtacgtt cctgtaaat agcctgaaa tattacttt 
      gctgtcgat acaaagtac gtgttgtca aagctgatg 
      ttactgttc ttccctttt acacataag gattggtcc 
      gttaagtat gagcaagat ggtcccgtc cagcctaga 
      ttcgagata aatgctcca gatctatat attccaaca 
      atggcttat gtaacttac gttttagtg gcggggatg 
      gttttaggc atgcaacaa aaattcacg cctgagcaa 
      atcgggatt ttggcttcg tcggcttta gcttggttt 
      gttgttgaa ttggcggtg tatagttgc actctttac 
      atagccaat atcaaaaca acacttaga acatttgat 
      ttgttagct ttcagtggt tataaattt gtggggatt 
      attgttagt attttggtg agcctgata ggcgcaaaa 
      acggcttat tactgttgt ttgatatac gtgaactta 
      gcgttagca tttttctta gtgcgaaca ttgaaagct 
      caagttctt gttgagagc aatgcccag cctacaagc 
      tattacggg gacgttgcc ccccctaca gggaacaag 
      cggaggtta tattttctg ttgtttgtg gcggcagtg 
      cagccagtg ctttcctgg tggttgtca tttcattta 
      atcggtagc ccaagccct gagaaacct gtaactgag 
      tga
    mRNA sequence
    • >TC010181 RA
      accaccaac tgtcaattt tgtaaagga agttgcaaa 
      ttacaaaac aaattatca tgctgtaat cggggtgtt 
      tgaaaaaat tattacact aattgtaat attatccca 
      acacactac attttaatc gggagtttg gtgctcaaa 
      caagctggg aaggagttt ggtcatgtg aaaaaatca 
      aaatgacag atgtctaag tgacgtgtc tctctgtgt 
      ttattcata caaaatcaa ctaatttaa gacttttta 
      catcgaaat ttatcaaaa ttgtcgtta tgaattaca 
      acgcgaata cagggaacc ggccagccc atagccgga 
      aagttaaaa gggttagtg acgtaaatg ccatggggt 
      acacaccgt acgatccca cgcaaggga tgccaaaca 
      atttgtacc ctaatttgg ggtccgaaa gccctgccc 
      caaacccat atcaacaaa attttgcaa gcccgtcgt 
      ttagttcaa cccaaaatt atggggtgc caccaaaca 
      acccccaaa tgtatgggt ttaatcccg cttcaccct 
      atccaaacg cacagccac ccccgaatc cgcagttcg 
      ggggcgttt ttggacaac caatggtcc aagacatgg 
      ccttgcagt atggacaac agttggcaa atacgggaa 
      agtcgatga taaaacaag aagtggaaa agtacgttc 
      ctgtaaata gcctgaaat attactttg ctgtcgata 
      caaagtacg tgttgtcaa agctgatgt tactgttct 
      tccctttta cacataagg attggtccg ttaagtatg 
      agcaagatg gtcccgtcc agcctagat tcgagataa 
      atgctccag atctatata ttccaacaa tggcttatg 
      taacttacg ttttagtgg cggggatgg ttttaggca 
      tgcaacaaa aattcacgc ctgagcaaa tcgggattt 
      tggcttcgt cggctttag cttggtttg ttgttgaat 
      tggcggtgt atagttgca ctctttaca tagccaata 
      tcaaaacaa cacttagaa catttgatt tgttagctt 
      tcagtggtt ataaatttg tggggatta ttgttagta 
      ttttggtga gcctgatag gcgcaaaaa cggcttatt 
      actgttgtt tgatatacg tgaacttag cgttagcat 
      ttttcttag tgcgaacat tgaaagctc aagttcttg 
      ttgagagca atgcccagc ctacaagct attacgggg 
      acgttgccc cccctacag ggaacaagc ggaggttat 
      attttctgt tgtttgtgg cggcagtgc agccagtgc 
      tttcctggt ggttgtcat ttcatttaa tcggtagcc 
      caagccctg agaaacctg taactgagt gaacttaac 
      tgcgcttgc taatttctt acatttgag cacctttaa 
      cctgttttt ttatgttaa cagtgagtt tatttgata 
      aatacatta tttttatgc ta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY