Status
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Gene information
General information
TC010176 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 13493167..13494841
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010176
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04778

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010176 RA
      MLKTLNISN FQIIRCLTS SVKPEIQSH LTLLETTYA 
      KDDFTNVTD KIASHLGRN LHTKEHHPL SHVRQRIVN 
      YFYKNFVNG RGNPTFSVY DALPPVVTV DQNFDSLLT 
      PKDHPSRLK SDCYYLNRD YLLRAHMTA HQSELMRAG 
      LNNFLMIGD VYRRDEIDR THYPVFHQV DAVRLKTRD 
      ELFPKEQSL EIFEVGNNT QSTGTQQKQ ACHTLEAVK 
      LMEHELKTT LAGLAKELF GETIEYRWI DTYFPFTQP 
      SWELEVFHE NNWLELLGC GIMRQPILT NSGIVDRIG 
      WAFGIGLER VAMCLYKIP DIRLFWSKD TGFLNQFKV 
      KYDEDVTYR QISQYPQCI NDLSFWLPQ DREFSSNDF 
      YDLARNIGG DTIEQVVLV DEFTHPKLG KTSHCYRII 
      YRHMEKTLV QADVNVIHS EIAKVAAST LGVQIR
    CDS sequence
    • >TC010176 RA
      atgttgaaa actctaaat attagcaat ttccaaatc 
      atcagatgt ctcacatca tctgtaaaa cccgaaatc 
      caatctcat ttaacatta ctagaaacc acatatgct 
      aaagacgac ttcacgaat gtcaccgat aaaatcgcc 
      tcgcacttg ggtcgcaat ctccacaca aaagaacac 
      caccccctt agccacgtg cgtcaaaga attgtaaat 
      tatttctac aaaaatttt gtcaacggc cgcggaaac 
      ccaactttc tcagtgtat gacgccctg cccccggtg 
      gtaaccgta gaccaaaac tttgatagc ttactgacc 
      cccaaagac cacccgtcc cgcttgaaa agcgactgt 
      tattaccta aaccgggac tatttattg cgggcgcat 
      atgacggcc caccagtcg gagctgatg agggccggt 
      ttgaacaac tttctaatg attggagac gtctacaga 
      cgggacgaa atcgacaga acgcactac ccggtcttc 
      caccaggtg gacgcagtt cgtctgaag acacgagac 
      gagttgttc cccaaagag caaagcttg gagattttt 
      gaggtgggc aacaacacc cagagcacc ggaacccag 
      cagaaacag gcgtgtcac actctggaa gcggtcaag 
      ttgatggaa cacgaactg aaaacgact ctggcaggc 
      cttgccaag gagctgttt ggggagaca attgagtac 
      aggtggatt gatacatat tttccgttc actcaaccg 
      tcatgggag ctggaggtg tttcacgag aacaactgg 
      ttagaactg ctgggatgt gggataatg agacagccc 
      attttaacc aactctggc attgtggac aggattggc 
      tgggcgttc gggatcggg cttgaaagg gtcgcaatg 
      tgtttgtat aaaataccc gatattcgg ctcttctgg 
      tccaaagac actgggttt ttaaaccag tttaaagta 
      aaatatgac gaagacgtt acttataga caaatcagt 
      caatatcca caatgtatc aacgatctc agtttttgg 
      ctaccacaa gatagggaa ttttcgagt aatgatttc 
      tacgacttg gccagaaac attggaggt gatacaatt 
      gaacaagta gttttagtg gacgaattc acgcatcca 
      aaacttggg aaaacgagt cactgttac cgcattatc 
      taccggcat atggaaaaa actctggtc caggccgat 
      gtcaatgta attcacagc gaaatcgcc aaagttgca 
      gcctccaca ttgggagtc cagattaga taa
    mRNA sequence
    • >TC010176 RA
      ctaggtaac tattgcagt tttaaaaac ggggatgat 
      gttttttgg aataagtgt tggtttcga ttttttaat 
      ttctgacta cgatcataa tgcctttcg aatttgcct 
      tcgacggtg ttgtttgtt aaaaagaat gatttggga 
      taacatata atagaatac aattgcaac gagtgattt 
      tatctcgtt ttttgtttt taatttttt tgcgccaaa 
      ttagaggcg cacgcgctg tagactttt ttgccttca 
      aattcgaag gttatgtaa ggttaggaa tgtgatgac 
      aggtcttta aaatgttga aaactctaa atattagca 
      atttccaaa tcatcagat gtctcacat catctgtaa 
      aacccgaaa tccaatctc atttaacat tactagaaa 
      ccacatatg ctaaagacg acttcacga atgtcaccg 
      ataaaatcg cctcgcact tgggtcgca atctccaca 
      caaaagaac accaccccc ttagccacg tgcgtcaaa 
      gaattgtaa attatttct acaaaaatt ttgtcaacg 
      gccgcggaa acccaactt tctcagtgt atgacgccc 
      tgcccccgg tggtaaccg tagaccaaa actttgata 
      gcttactga cccccaaag accacccgt cccgcttga 
      aaagcgact gttattacc taaaccggg actatttat 
      tgcgggcgc atatgacgg cccaccagt cggagctga 
      tgagggccg gtttgaaca actttctaa tgattggag 
      acgtctaca gacgggacg aaatcgaca gaacgcact 
      acccggtct tccaccagg tggacgcag ttcgtctga 
      agacacgag acgagttgt tccccaaag agcaaagct 
      tggagattt ttgaggtgg gcaacaaca cccagagca 
      ccggaaccc agcagaaac aggcgtgtc acactctgg 
      aagcggtca agttgatgg aacacgaac tgaaaacga 
      ctctggcag gccttgcca aggagctgt ttggggaga 
      caattgagt acaggtgga ttgatacat attttccgt 
      tcactcaac cgtcatggg agctggagg tgtttcacg 
      agaacaact ggttagaac tgctgggat gtgggataa 
      tgagacagc ccattttaa ccaactctg gcattgtgg 
      acaggattg gctgggcgt tcgggatcg ggcttgaaa 
      gggtcgcaa tgtgtttgt ataaaatac ccgatattc 
      ggctcttct ggtccaaag acactgggt ttttaaacc 
      agtttaaag taaaatatg acgaagacg ttacttata 
      gacaaatca gtcaatatc cacaatgta tcaacgatc 
      tcagttttt ggctaccac aagataggg aattttcga 
      gtaatgatt tctacgact tggccagaa acattggag 
      gtgatacaa ttgaacaag tagttttag tggacgaat 
      tcacgcatc caaaacttg ggaaaacga gtcactgtt 
      accgcatta tctaccggc atatggaaa aaactctgg 
      tccaggccg atgtcaatg taattcaca gcgaaatcg 
      ccaaagttg cagcctcca cattgggag tccagatta 
      gataatgtc acagttaat ccctattta attaagcag 
      catattgtt gctcaaata attgtaaaa tattgcgaa 
      ataaagatt acattttag g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY