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Gene information
General information
TC010172 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 13643089..13645241
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010172
    iBeetle screen iB_04777

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    egg chamber not present - Number of animals showing the phenotype: 4

    tropharium shortened - Number of animals showing the phenotype: 4

    vitellarium shortened - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection



    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010172 RA
      MVKSPAVGI DLGTTYSCV GVWQHGKVE IIANDQGNR 
      TTPSYVAFT DTERLLGDA AKNQVAMNP SNTIFDAKR 
      LIGRKFDDP KIQQDIKHW PFKVINDCG KPKIQVEHK 
      GEIKKFAPE EISSMVLTK MKETAEAYL GTSVRDAVI 
      TVPAYFNDS QRQATKDAG VIAGLNVMR IINEPTAAA 
      LAYGLDKNL KGERNVLIF DLGGGTFDV SILTIDEGS 
      LFEVKATAG DTHLGGEDF DNRLVNHLA DEFKRKYKK 
      DLRSNPRAL RRLRTAAER AKRTLSSST EASIEIDAL 
      FDGIDFYTK ISRARFEEM NADLFRGTL QPVEKALTD 
      AKMDKGMIH DIVLVGGST RIPKIQQLL QNYFNGKPL 
      NLSINPDEA VAYGAAVQA AVLSGETDS KIQDVLLVD 
      VTPLSLGIE TAGGVMTKI IERNARIPC KQTQTFTTY 
      ADNQPAVTI QVFEGERAM TKDNNLLGT FDLTGIPPA 
      PRGVPKIEV TFDLDANGI LNVSAKDTS SGNSRNITI 
      KNDKGRLSQ KDIDRMVAE AEQYKEEDE KQRQRIAAR 
      NQLEGYIFQ LKQTISEQG SKLSEADKE TVTRECDSC 
      LQWLDANTL AEKEEYEDK QKQLSSICA PIMAKLYQQ 
      GGQGGMPGA GGSCGQQAG GFGGQRQGG PTIEEVD
    CDS sequence
    • >TC010172 RA
      atggtgaaa tctccagca gttggtatc gacttggga 
      accacctac tcctgcgtg ggagtctgg cagcatgga 
      aaagtcgag atcatcgcc aacgaccaa ggtaacaga 
      accacccca agttatgtt gccttcacc gacaccgag 
      cgtcttctc ggcgacgct gcgaagaac caagttgcc 
      atgaacccc agcaacacc atcttcgac gcgaaacga 
      ttaattgga aggaaattc gacgacccg aagatccag 
      caagatatc aaacactgg ccgtttaaa gttatcaat 
      gactgcggc aaacccaag atccaagtc gagcacaaa 
      ggcgaaatt aagaagttt gcaccggag gagatcagt 
      tcgatggtg ctgacgaaa atgaaggaa accgctgaa 
      gcgtatttg ggaacatct gtaagggat gctgttatc 
      acagtcccg gcatatttc aacgactcc cagaggcaa 
      gctacgaaa gatgccggt gttatcgcc ggtttgaac 
      gtcatgagg atcatcaat gaaccgaca gcagcggct 
      ttggcttat gggcttgac aagaatttg aagggcgaa 
      cgaaacgtc cttattttc gacctgggt ggcggcact 
      ttcgatgtt tccatctta accatcgac gaagggtcg 
      ttgttcgaa gttaaagcc actgcaggt gacacccac 
      ctgggtggt gaagacttc gacaaccgc cttgttaac 
      cacttggcc gacgaattc aaacgcaaa tacaagaaa 
      gatctgaga agcaatccg agggcttta cggcgattg 
      agaaccgcc gctgaaaga gcgaagcga accttgtct 
      tcaagcacc gaagcatcg attgaaatc gacgccctc 
      ttcgacgga atcgacttc tacaccaag atcagtcga 
      gcaaggttc gaagaaatg aacgccgat ttgttcagg 
      gggacacta caacctgtc gagaaagcc ctgaccgac 
      gctaaaatg gacaaggga atgatccac gacatcgtc 
      ctcgtcggc ggctccacc cgaatcccg aaaatccaa 
      caactgctc cagaactat ttcaacgga aaaccactg 
      aatctctca attaaccct gacgaagcc gtcgcctac 
      ggtgccgcc gtccaagcg gctgtcttg agcggtgaa 
      accgattcg aagatccag gacgtgctc ctcgtagac 
      gtgactccc ctatcacta ggtatcgaa accgccgga 
      ggcgtgatg acgaagatc atcgaacgc aacgcaaga 
      atcccatgc aagcaaacg caaaccttc acaacttac 
      gccgacaac caaccggcc gtcaccatc caagtcttc 
      gagggtgaa agagccatg acaaaagac aacaacctt 
      ttgggaacc ttcgattta acgggaatt ccaccagca 
      ccacgtgga gtcccgaaa atcgaagtc actttcgac 
      ctggacgcc aacggcatc ctgaacgtc tccgctaaa 
      gacaccagc tccggtaat tcacgcaat attaccatc 
      aagaacgac aaaggaaga ttatcacag aaagacatc 
      gataggatg gtggcagag gctgaacaa tataaagag 
      gaggatgag aagcagaga cagagaatt gcggccagg 
      aatcaatta gagggttac atcttccaa ttgaaacag 
      actatttcc gaacaaggg agcaaatta agcgaagct 
      gacaaagaa actgtaacg agggaatgt gacagctgc 
      ttgcagtgg ttggatgcc aacactctg gccgagaag 
      gaggaatat gaagataaa cagaaacaa ttatcgtcc 
      atctgtgct ccaattatg gctaaattg taccaacaa 
      ggcggtcag ggaggtatg cctggggct ggaggaagc 
      tgtggacaa caggccggc ggctttgga ggtcaacga 
      caaggagga ccaacaatt gaagaagtt gattaa
    mRNA sequence
    • >TC010172 RA
      tttgaattc aagcgattg aacaagcaa gtcaagcaa 
      gcgaagcta acaaagcgt tttctacaa agtgtttac 
      aagtgaatt tgaatcgaa gcaaagcaa atcagagaa 
      aatggtgaa atctccagc agttggtat cgacttggg 
      aaccaccta ctcctgcgt gggagtctg gcagcatgg 
      aaaagtcga gatcatcgc caacgacca aggtaacag 
      aaccacccc aagttatgt tgccttcac cgacaccga 
      gcgtcttct cggcgacgc tgcgaagaa ccaagttgc 
      catgaaccc cagcaacac catcttcga cgcgaaacg 
      attaattgg aaggaaatt cgacgaccc gaagatcca 
      gcaagatat caaacactg gccgtttaa agttatcaa 
      tgactgcgg caaacccaa gatccaagt cgagcacaa 
      aggcgaaat taagaagtt tgcaccgga ggagatcag 
      ttcgatggt gctgacgaa aatgaagga aaccgctga 
      agcgtattt gggaacatc tgtaaggga tgctgttat 
      cacagtccc ggcatattt caacgactc ccagaggca 
      agctacgaa agatgccgg tgttatcgc cggtttgaa 
      cgtcatgag gatcatcaa tgaaccgac agcagcggc 
      tttggctta tgggcttga caagaattt gaagggcga 
      acgaaacgt ccttatttt cgacctggg tggcggcac 
      tttcgatgt ttccatctt aaccatcga cgaagggtc 
      gttgttcga agttaaagc cactgcagg tgacaccca 
      cctgggtgg tgaagactt cgacaaccg ccttgttaa 
      ccacttggc cgacgaatt caaacgcaa atacaagaa 
      agatctgag aagcaatcc gagggcttt acggcgatt 
      gagaaccgc cgctgaaag agcgaagcg aaccttgtc 
      ttcaagcac cgaagcatc gattgaaat cgacgccct 
      cttcgacgg aatcgactt ctacaccaa gatcagtcg 
      agcaaggtt cgaagaaat gaacgccga tttgttcag 
      ggggacact acaacctgt cgagaaagc cctgaccga 
      cgctaaaat ggacaaggg aatgatcca cgacatcgt 
      cctcgtcgg cggctccac ccgaatccc gaaaatcca 
      acaactgct ccagaacta tttcaacgg aaaaccact 
      gaatctctc aattaaccc tgacgaagc cgtcgccta 
      cggtgccgc cgtccaagc ggctgtctt gagcggtga 
      aaccgattc gaagatcca ggacgtgct cctcgtaga 
      cgtgactcc cctatcact aggtatcga aaccgccgg 
      aggcgtgat gacgaagat catcgaacg caacgcaag 
      aatcccatg caagcaaac gcaaacctt cacaactta 
      cgccgacaa ccaaccggc cgtcaccat ccaagtctt 
      cgagggtga aagagccat gacaaaaga caacaacct 
      tttgggaac cttcgattt aacgggaat tccaccagc 
      accacgtgg agtcccgaa aatcgaagt cactttcga 
      cctggacgc caacggcat cctgaacgt ctccgctaa 
      agacaccag ctccggtaa ttcacgcaa tattaccat 
      caagaacga caaaggaag attatcaca gaaagacat 
      cgataggat ggtggcaga ggctgaaca atataaaga 
      ggaggatga gaagcagag acagagaat tgcggccag 
      gaatcaatt agagggtta catcttcca attgaaaca 
      gactatttc cgaacaagg gagcaaatt aagcgaagc 
      tgacaaaga aactgtaac gagggaatg tgacagctg 
      cttgcagtg gttggatgc caacactct ggccgagaa 
      ggaggaata tgaagataa acagaaaca attatcgtc 
      catctgtgc tccaattat ggctaaatt gtaccaaca 
      aggcggtca gggaggtat gcctggggc tggaggaag 
      ctgtggaca acaggccgg cggctttgg aggtcaacg 
      acaaggagg accaacaat tgaagaagt tgattaagc 
      attccttaa gaattttaa ctttaattt ctcttaaaa 
      acctttttt ttctaatag tattatgtt aaatgcagt 
      cactattga aataatgtg atattgtta tg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY