Status
Please wait ...
Gene information
General information
TC010166 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 13693294..13696221
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010166
    iBeetle screen iB_04774

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010166 RA
      MCENGAEYT DTVDPRVHV ELERLNNAT DEINKLEVE 
      LDEARNNFR QLLCDSTAR VDALRVKLG MCVERAKPY 
      YEARFCANE ALKQTQVAA MKFERANSA HSAAREMVY 
      LAEQGLGGR TLDPAWQEM LNHATQRVN DAERERGIA 
      GTEHRIACM KHEASNAKV QSLQKELKR AIAKSSLSI 
      RRSFMTMSN IFAQHELVL LPYYEMKAH FNHLLEQQK 
      SKVQHYESQ VAAAKLTYA EALRNLEQI SDEIHQTRR 
      KASLLPPPN KQRSQDSNE SFLDDAQEF VDEYKSLPQ 
      TLGASASPI MSRMDDVEG YKNIRLNGS PGSPIENPS 
      VAQSHSSEW TEINLDSSS PEDDVPYRK LQGEEGEKP 
      RLIRQKTLP NPSIENEFS SIKSKMKLD TSISNWISR 
      SSAKNEGDS LNSSRRQSL DNILGPTGE KVKEIFSHG 
      MMMLNISSL TERRNSEPK PGLTEEKTR SSAKKIPSP 
      LEKTLTYLT AEEDNSDTE SLSSVDMLN EDQISSLML 
      DKDIGVVCE QVLGTPISE VVSLPQCAS SSHPSK
    CDS sequence
    • >TC010166 RA
      atgtgcgaa aatggggcc gaatacact gacacagtt 
      gacccaagg gtccacgta gaattagag cgtttaaat 
      aatgccact gatgaaatt aacaaattg gaagtcgaa 
      ttagacgaa gctaggaac aacttccgc caattacta 
      tgtgatagc acggcccga gtggacgct ctgcgagtg 
      aagctcggg atgtgcgtt gagagggcg aagccctac 
      tatgaggcc aggttttgc gcgaacgaa gcgttgaag 
      cagacgcaa gttgcggcc atgaagttt gagagggct 
      aatagtgca cacagtgca gctagggag atggtttat 
      ttggccgaa cagggatta ggggggcgc actttagat 
      cccgcatgg caagaaatg ctaaatcat gctactcaa 
      agagttaac gatgctgag cgtgagaga ggcattgca 
      ggaactgag cacaggatc gcttgtatg aaacatgaa 
      gcatctaat gctaaagta caaagctta caaaaggaa 
      ctgaaaagg gctatcgct aaaagcagt ttatccatt 
      agaaggtcc ttcatgaca atgagtaat atttttgca 
      caacacgaa ttggtgcta ctgccctat tatgagatg 
      aaggcccac tttaaccac ctcctcgag cagcagaag 
      tccaaagtt caacactac gagtctcaa gtggcagcc 
      gcaaagctc acttacgcc gaagccctc agaaatctg 
      gagcaaata tctgatgag atccaccaa actagaaga 
      aaggcctct ttgctgcct cctcccaac aagcaaaga 
      agccaagat tccaacgag tcattcctg gatgatgct 
      caagagttt gtagatgaa tataagagt cttccacaa 
      acgttggga gcctcagct agtccaatt atgtccagg 
      atggacgat gtagagggt tataagaat attaggttg 
      aatgggtcg cctggatcg cccattgaa aatccaagc 
      gttgcccaa agtcattcc agtgaatgg actgaaatt 
      aatctggat tcttccagt cctgaagac gacgtacct 
      tataggaaa ttgcaggga gaggaagga gagaagccg 
      agattgatc agacagaaa actttgccc aatccgagt 
      attgagaat gagttcagt tcgattaaa agcaagatg 
      aaactggat acgagcatt tcgaactgg atctcaagg 
      tcttcagcg aagaacgaa ggggattca ttaaattca 
      agtcggcga cagagttta gataacatt ttgggtcca 
      acgggtgag aaggtcaaa gagatattt tcccatggc 
      atgatgatg ctaaacata agctctttg actgaacgg 
      aggaacagt gaacctaag cctggtttg actgaggag 
      aaaacaaga agcagtgcc aagaaaatt ccaagtcct 
      ttggagaaa actttgaca tatctcacg gcagaagag 
      gataattct gatactgaa agcctctcc agcgttgat 
      atgttaaat gaggatcaa ataagctcg ttgatgctg 
      gacaaagac attggggtt gtctgtgag caggtttta 
      gggacgccg atctcggaa gtggtttcg ttgccgcaa 
      tgcgcttct tctagtcat ccaagcaaa taa
    mRNA sequence
    • >TC010166 RA
      tcaacctca aattttgtt tttatttct taatttatt 
      attccactc ataaagtaa gcactaaca atgcagtaa 
      aaaagtacc tcgaccaca aattcaata accatcaag 
      ggaacggct tgacaatcc tcgaatctg tgtataatg 
      tgcgaaaat ggggccgaa tacactgac acagttgac 
      ccaagggtc cacgtagaa ttagagcgt ttaaataat 
      gccactgat gaaattaac aaattggaa gtcgaatta 
      gacgaagct aggaacaac ttccgccaa ttactatgt 
      gatagcacg gcccgagtg gacgctctg cgagtgaag 
      ctcgggatg tgcgttgag agggcgaag ccctactat 
      gaggccagg ttttgcgcg aacgaagcg ttgaagcag 
      acgcaagtt gcggccatg aagtttgag agggctaat 
      agtgcacac agtgcagct agggagatg gtttatttg 
      gccgaacag ggattaggg gggcgcact ttagatccc 
      gcatggcaa gaaatgcta aatcatgct actcaaaga 
      gttaacgat gctgagcgt gagagaggc attgcagga 
      actgagcac aggatcgct tgtatgaaa catgaagca 
      tctaatgct aaagtacaa agcttacaa aaggaactg 
      aaaagggct atcgctaaa agcagttta tccattaga 
      aggtccttc atgacaatg agtaatatt tttgcacaa 
      cacgaattg gtgctactg ccctattat gagatgaag 
      gcccacttt aaccacctc ctcgagcag cagaagtcc 
      aaagttcaa cactacgag tctcaagtg gcagccgca 
      aagctcact tacgccgaa gccctcaga aatctggag 
      caaatatct gatgagatc caccaaact agaagaaag 
      gcctctttg ctgcctcct cccaacaag caaagaagc 
      caagattcc aacgagtca ttcctggat gatgctcaa 
      gagtttgta gatgaatat aagagtctt ccacaaacg 
      ttgggagcc tcagctagt ccaattatg tccaggatg 
      gacgatgta gagggttat aagaatatt aggttgaat 
      gggtcgcct ggatcgccc attgaaaat ccaagcgtt 
      gcccaaagt cattccagt gaatggact gaaattaat 
      ctggattct tccagtcct gaagacgac gtaccttat 
      aggaaattg cagggagag gaaggagag aagccgaga 
      ttgatcaga cagaaaact ttgcccaat ccgagtatt 
      gagaatgag ttcagttcg attaaaagc aagatgaaa 
      ctggatacg agcatttcg aactggatc tcaaggtct 
      tcagcgaag aacgaaggg gattcatta aattcaagt 
      cggcgacag agtttagat aacattttg ggtccaacg 
      ggtgagaag gtcaaagag atattttcc catggcatg 
      atgatgcta aacataagc tctttgact gaacggagg 
      aacagtgaa cctaagcct ggtttgact gaggagaaa 
      acaagaagc agtgccaag aaaattcca agtcctttg 
      gagaaaact ttgacatat ctcacggca gaagaggat 
      aattctgat actgaaagc ctctccagc gttgatatg 
      ttaaatgag gatcaaata agctcgttg atgctggac 
      aaagacatt ggggttgtc tgtgagcag gttttaggg 
      acgccgatc tcggaagtg gtttcgttg ccgcaatgc 
      gcttcttct agtcatcca agcaaataa atgagtaca 
      attaataag gaaagatat tattttttg gtcgagtta 
      ggtagacat atcagtgga ttttatttt tgagttcta 
      gtcatttca agctgcaca tggactgta aatgcaata 
      atacctgcc tctagctgc tattatgat gtaataaat 
      taacgcttg tatctgtgg tagagctag tcgatgagt 
      ttaaagcaa tattgtaaa agtttaaga ttgttatcg 
      aattttatc tgaataaat acctgaaga caaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY