Status
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Gene information
General information
TC033857
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010158,TC010159
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 13823150..13831255
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033857
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04772

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    larva appendages partially irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head appendages random not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head appendages partially shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs partially shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen partially everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033857 RA
      MHPFYKILI LALIVYLAE LNILPQLSP SFKCKDPKI 
      SKKFTGDTV TATNLLIVT YFGPLILIP LIEVLRQNS 
      FKKINSGAV KTLYKNYLV GSLFVILLT ELLKRIVNE 
      ARPHFLHTC KPDTNELCQ PGTLISDYI CTNLQLSTS 
      SAKDLMRSF PSGHTSLST FGGFYCAYI VHTRLSKIR 
      GSLARLFLI AVCVSWSVG CSMSRIVDK KHHWWDVLA 
      GATLGTCCV FYTLRLLNK NKLLDSAAP SSKAQGTCC 
      DDPRALHPH GKPFLRYDA KVYGREMIA GPGGRLLVT 
      GKPEYFNQV LASLAVSLG TLTAGLGKG YSSPAIASL 
      QECDADAFP VSRQEASWL ASLSILGAF FGALLGCAA 
      VRWGRRRTL LLTGIPLSV SWLIIAFAV SVEVMCFAA 
      FISGFLIAI VQLAAQVYV SEIAAPSIR GGLSGMLKV 
      AGHAGVLVA YAAGAFLDW RQLAWLIAV APAMMCVAM 
      WKVPESPGF LVLRGRDDE AEKALRSVT SSHRVLLRT 
      LMTPGLIAC GLMFFQRFS GANAFYFYV VTVFKETFG 
      GTNPHSAAV AVAVVQFLS SLLSGLLVD SVGRLPLLV 
      ASSVLMSLA LASFGSFAY YEDAHHKNV PNLDWIPLL 
      CVLVFTVAF SLGISPISW LLVTELFSL EHRGLGTAL 
      ATAFSHLCS FIGVKTFVD FKELLGLHG AFWLYAAIS 
      VCGLCFVVC CVPETKGRD LTEIDTER
    CDS sequence
    • >TC033857 RA
      atgcatcct ttctacaaa atcctaatt cttgcttta 
      atagtgtat ttagcggaa ctaaatata ctgccacaa 
      ttaagcccc agtttcaaa tgcaaggac ccaaaaatt 
      tcgaaaaaa ttcaccggt gacactgtc actgccaca 
      aacctgctc atcgtgact tacttcgga ccattaata 
      ctcatccca ctgatcgaa gttctgaga caaaattcc 
      ttcaagaaa atcaattcg ggggcggtg aaaactttg 
      tacaaaaat tacctcgtg gggagctta ttcgtaatt 
      ttgctaaca gaactttta aaacgtatt gtaaatgag 
      gctcgacca cacttcctg cacacgtgc aaaccagac 
      acgaacgag ttatgccaa cctgggacc ctgatttct 
      gactatatc tgcaccaac ctgcagctt agtacttcg 
      agtgccaag gacttgatg aggtccttt ccctcagga 
      catacctcg ctgtccaca tttggcggc ttttactgc 
      gcttatatt gtccacaca aggctttct aaaattcgc 
      ggaagtttg gcacgacta tttctcatt gcggtatgt 
      gttagttgg agtgttgga tgctcgatg tcaaggata 
      gtagacaag aagcaccac tggtgggat gttttggca 
      ggagccact ctaggaacg tgctgcgtc ttttacact 
      ttacgcctc ttgaataaa aataaactt ttggacagt 
      gcggcaccc agttccaag gcgcaaggc acgtgctgc 
      gatgacccg agagcttta cacccacac ggtaagccc 
      ttcctgcga tacgatgca aaagtttac ggccgggaa 
      atgatcgcg ggacctgga ggccgcctc ctcgtaacc 
      ggcaaaccc gaatacttc aaccaagtg ctcgcctcg 
      ctcgccgtc tccctcggc actctcact gccggcctg 
      ggcaagggc tacagctcc ccggcgatc gcctctttg 
      caggaatgc gacgccgac gccttcccc gtctcacgc 
      caggaagcc agctggctc gccagcctc tcgatcctg 
      ggcgccttc ttcggcgcc ctcctcggc tgcgccgcc 
      gtccgctgg ggccgccgc cgcaccctc ctcctcacc 
      ggcatcccg ctctccgtc tcctggctc ataatcgcg 
      ttcgccgtg agcgtggag gtcatgtgc ttcgccgcc 
      ttcatcagc ggcttcctc atcgccatc gtccagctc 
      gccgctcaa gtatacgta agcgagatc gccgccccc 
      tccatccgg ggcggcctc agcggcatg ctcaaggtg 
      gcggggcac gcgggggtc ctggtggcc tacgccgcc 
      ggggccttc ctggactgg cggcagctc gcctggctc 
      atcgccgtg gccccggcc atgatgtgc gtggccatg 
      tggaaggtg cccgaaagc ccgggtttc ctggtgctc 
      cgggggcgc gacgacgag gccgagaag gcgctgcgg 
      agcgttacg tcgagtcac agagtcctg ctccggact 
      ctgatgact ccggggcta atcgcttgc ggtttgatg 
      ttcttccag cgattttcc ggagctaac gcgttctat 
      ttttacgtc gtaacggtc tttaaggag actttcgga 
      ggcacgaac ccccacagc gctgcggtg gccgtcgct 
      gttgtccaa ttcttgtca tctctgttg tccgggctt 
      ttggtggat agtgtcggg cgtttgccc cttcttgtc 
      gcgtccagt gtcttgatg tcgcttgct ttggcctcg 
      ttcgggtcc ttcgcttac tacgaagac gcgcatcac 
      aagaacgtt ccaaacttg gactggatt cccctgctt 
      tgtgtgttg gtatttacc gttgccttc tccctgggg 
      atcagtccg atctcgtgg ctgctcgtc accgaattg 
      ttttcgctg gaacatcgc ggtttgggc accgctcta 
      gctaccgct ttcagccac ctttgctcg ttcattgga 
      gtcaaaacg ttcgtcgat tttaaggag ctgctgggg 
      ctgcacggg gcgttttgg ctgtatgca gcgatttcg 
      gtctgcggt ttgtgcttt gttgtgtgc tgcgtcccg 
      gaaaccaaa ggaagggat ctcacggag attgatacg 
      gagaggtga
    mRNA sequence
    • >TC033857 RA
      gtttttgcg taatttggt agaaatgtg gaaaattat 
      tatcatcca ataatagaa gtacttcag gttagagcg 
      tttatagtg tatttaatt catttcaca ctcgtgtac 
      gcgctcaaa gactataca taatgcatc ctttctaca 
      aaatcctaa ttcttgctt taatagtgt atttagcgg 
      aactaaata tactgccac aattaagcc ccagtttca 
      aatgcaagg acccaaaaa tttcgaaaa aattcaccg 
      gtgacactg tcactgcca caaacctgc tcatcgtga 
      cttacttcg gaccattaa tactcatcc cactgatcg 
      aagttctga gacaaaatt ccttcaaga aaatcaatt 
      cgggggcgg tgaaaactt tgtacaaaa attacctcg 
      tggggagct tattcgtaa ttttgctaa cagaacttt 
      taaaacgta ttgtaaatg aggctcgac cacacttcc 
      tgcacacgt gcaaaccag acacgaacg agttatgcc 
      aacctggga ccctgattt ctgactata tctgcacca 
      acctgcagc ttagtactt cgagtgcca aggacttga 
      tgaggtcct ttccctcag gacatacct cgctgtcca 
      catttggcg gcttttact gcgcttata ttgtccaca 
      caaggcttt ctaaaattc gcggaagtt tggcacgac 
      tatttctca ttgcggtat gtgttagtt ggagtgttg 
      gatgctcga tgtcaagga tagtagaca agaagcacc 
      actggtggg atgttttgg caggagcca ctctaggaa 
      cgtgctgcg tcttttaca ctttacgcc tcttgaata 
      aaaataaac ttttggaca gtgcggcac ccagttcca 
      aggcgcaag gcacgtgct gcgatgacc cgagagctt 
      tacacccac acggtaagc ccttcctgc gatacgatg 
      caaaagttt acggccggg aaatgatcg cgggacctg 
      gaggccgcc tcctcgtaa ccggcaaac ccgaatact 
      tcaaccaag tgctcgcct cgctcgccg tctccctcg 
      gcactctca ctgccggcc tgggcaagg gctacagct 
      ccccggcga tcgcctctt tgcaggaat gcgacgccg 
      acgccttcc ccgtctcac gccaggaag ccagctggc 
      tcgccagcc tctcgatcc tgggcgcct tcttcggcg 
      ccctcctcg gctgcgccg ccgtccgct ggggccgcc 
      gccgcaccc tcctcctca ccggcatcc cgctctccg 
      tctcctggc tcataatcg cgttcgccg tgagcgtgg 
      aggtcatgt gcttcgccg ccttcatca gcggcttcc 
      tcatcgcca tcgtccagc tcgccgctc aagtatacg 
      taagcgaga tcgccgccc cctccatcc ggggcggcc 
      tcagcggca tgctcaagg tggcggggc acgcggggg 
      tcctggtgg cctacgccg ccggggcct tcctggact 
      ggcggcagc tcgcctggc tcatcgccg tggccccgg 
      ccatgatgt gcgtggcca tgtggaagg tgcccgaaa 
      gcccgggtt tcctggtgc tccgggggc gcgacgacg 
      aggccgaga aggcgctgc ggagcgtta cgtcgagtc 
      acagagtcc tgctccgga ctctgatga ctccggggc 
      taatcgctt gcggtttga tgttcttcc agcgatttt 
      ccggagcta acgcgttct atttttacg tcgtaacgg 
      tctttaagg agactttcg gaggcacga acccccaca 
      gcgctgcgg tggccgtcg ctgttgtcc aattcttgt 
      catctctgt tgtccgggc ttttggtgg atagtgtcg 
      ggcgtttgc cccttcttg tcgcgtcca gtgtcttga 
      tgtcgcttg ctttggcct cgttcgggt ccttcgctt 
      actacgaag acgcgcatc acaagaacg ttccaaact 
      tggactgga ttcccctgc tttgtgtgt tggtattta 
      ccgttgcct tctccctgg ggatcagtc cgatctcgt 
      ggctgctcg tcaccgaat tgttttcgc tggaacatc 
      gcggtttgg gcaccgctc tagctaccg ctttcagcc 
      acctttgct cgttcattg gagtcaaaa cgttcgtcg 
      attttaagg agctgctgg ggctgcacg gggcgtttt 
      ggctgtatg cagcgattt cggtctgcg gtttgtgct 
      ttgttgtgt gctgcgtcc cggaaacca aaggaaggg 
      atctcacgg agattgata cggagaggt gatcaaacg 
      ccaatgggg gaaaacgca aaatttcca ttagcgtag 
      tggagaggt attgctgat ttttgttat cagaatctt 
      ttgtgataa cgaaacgta caatcgtga tattttccc 
      ccaaaatca ggtttcttt catatcgag aaatttgtg 
      aaattcaat taaagaaaa caatctttg gcgccgagc 
      ttcactata tcaaaaatt attactaat ctagtttta 
      atcaattgt ggtccattt tgacaatac caccatatt 
      tgacaatat ttattagac aatattata atgttagag 
      tgattgact tatatcttt aaaacactt aggtgctat 
      ttagaagct ttagcaaag ataatcaat taatgtttc 
      aacctgatt aggttctag atgatgcaa tgtccattg 
      tcattccct tacaaatcc acttggatc ccgttgctt 
      taattacaa agatcaaca ataattaaa taaattaat 
      ggcaaacac taatttaca ttgctgcag taaatgagg 
      aagccttca ataatactg tttgtcatt ataatctga 
      acagctgga ttcaccgtg gtttaataa gttgggcgc 
      tgttatgta aaatgaaat cacccatcc tctgcctct 
      taaacgtaa ataatagtg tgtcaaagc ttgaagcat 
      ccaatagtt gtgtcagtg tctaagtgt tgattcgca 
      aaaaattac attttgcat aaccgtgtc caaataaga 
      taaagctca atttacatt agcccaatt ccttgacaa 
      tattttaca atgtagtgt gtaagagtt tatcagtaa 
      aaatttatt atctactcg ctggagttg ttgatgaat 
      tttcccatt gttgactat aaagaaaaa agcgttaac 
      aac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY