Status
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Gene information
General information
TC010157 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 13831580..13838415
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010157
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04771

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010157 RA
      MFARQFIRF ASYDSTRKN LKLTKASKL ICQGFTGKQ 
      ATFHCERAL EYGTLLVGG VTPGKGGKK HLGLPVFNT 
      VKEARDALK PDVSIIYVP PFAATKALH EALEAEIPL 
      IVCITEGIP QHDMVKFRY RLSRQDKSR LVGPNCPGI 
      IAPEQCKVG IMPAMIHKR GCIGIVSRS GTLTYEAVN 
      QTTKHGLGQ TLVVGIGGD PYNGTNFID CLDIFLKDP 
      DTKGIILIG EIGGCAEEE AAEFLKKNN TGDKRKPVV 
      SYIAGLSAP PGRRMGHAG AIISGGKGG APEKMAALR 
      NAGVIIANS PALMGDHLK TEMDNKIMM VTAPSEGTL 
      DEVITKNIA GLDRNINLQ KQTPLQKLR KRLRRRILV 
      DTLIFVIVA LLVCLVEFG IIPNTKLGF TCKDPKLSY 
      PFKGDTVSV TTLLISAYF GPILIITLV EVLKEHSFK 
      KVRLGTVWN YYRECLIGA TFVLLITEV IKVIVGEPR 
      PHFLDSCEP DVKCKEGAF IVEYNCTNT RLSNFFLSD 
      ITRSFPSGH TSVSLFIAL YCSYLIQIR IPSKSVSTL 
      TKPFLIAVC LTWCLLCSL SRITDRRHH WWDVLGGAF 
      LGLCGALYT LSLLHSKET KEKNRLSRT STSMTIMSE 
      KSKDTPSVV I
    CDS sequence
    • >TC010157 RA
      atgtttgca agacagttc atccgcttc gccagttac 
      gattcaacc cggaaaaat ctaaagctc acaaaagcc 
      tcaaagctc atatgtcaa ggcttcacg ggcaagcaa 
      gcaacattt cactgcgaa cgtgccctc gaatacggc 
      actctcctc gtcgggggc gtaactccg ggcaaaggg 
      ggcaagaaa catttaggc ctccctgtc ttcaacaca 
      gttaaggaa gcccgagac gccttaaaa cccgacgtt 
      agcataatc tacgtccct cccttcgcc gccactaaa 
      gccctacat gaagcccta gaggccgaa atcccccta 
      atagtgtgc ataaccgaa ggcataccc caacacgac 
      atggtcaag tttcgctac agattatcc cgccaagac 
      aaatccaga ttagtcggc ccgaactgc cccggaatt 
      atcgcccct gagcagtgc aaagtgggc ataatgccc 
      gcgatgatc cacaaaagg gggtgcatt gggatcgtg 
      tccaggtcc gggactctg acctacgag gcagtgaac 
      caaacgacc aagcatggg ctgggtcag accctggtt 
      gtgggcatt gggggagat ccatacaat gggacgaat 
      ttcatcgac tgtcttgat atttttctc aaagatcca 
      gacacaaaa ggaattatt ttaattggc gaaattggt 
      ggatgcgcc gaagaagaa gccgccgaa tttttaaag 
      aagaataat acaggggat aagagaaag ccggtagtt 
      agttacatt gcgggactt tcggcacct ccaggtcga 
      cgaatgggg cacgccggt gctattatt tcgggagga 
      aagggggga gccccggag aaaatggcg gctttgcga 
      aacgcggga gttattatt gcaaactcg cccgcgttg 
      atgggggac catttaaaa accgaaatg gataataaa 
      ataatgatg gttacagcg ccctctgaa ggaacgctc 
      gacgaagtc atcacgaaa aatatagca ggactcgat 
      agaaacatc aacctgcag aagcaaaca ccgttacaa 
      aaactgcgc aagcgttta cgtagacgg attttggtt 
      gatacgttg atatttgta atagttgcc ttattagta 
      tgtctagtc gaattcggc atcatcccc aatacaaaa 
      cttggcttc acttgcaag gacccgaag ctttcctat 
      ccctttaaa ggggacaca gtttccgtg acaacttta 
      ctgataagt gcttatttc ggacccatt cttatcata 
      accctagtg gaagtattg aaagaacac tcgttcaag 
      aaagtgcgt cttgggacc gtgtggaac tactacagg 
      gaatgcctt atcggcgcc acttttgtt cttctgata 
      acagaagta attaaagtc atcgtggga gagccccga 
      ccgcatttc ttggactcg tgtgagccc gacgttaag 
      tgcaaagaa ggcgctttc atagtcgaa tacaactgc 
      accaacacc cgcttgagc aactttttc ttgagcgac 
      atcacgaga tcttttccc tcaggacac acttccgtc 
      tccttgttc atcgctctt tattgctcg tatctcatc 
      caaattcgc attccctct aaatccgta agcactttg 
      accaaaccc ttcttgatc gctgtttgc ctcacatgg 
      tgtctgtta tgttccttg tcgaggata actgatcgg 
      cggcatcac tggtgggat gttctgggt ggggcgttt 
      ctgggactg tgtggtgca ctttacacg ttgagtctg 
      ctccatagc aaggaaacg aaagagaaa aaccgcctc 
      agcagaacc agcacgagt atgacaata atgtcagag 
      aagagcaaa gacactcca agtgttgtt atataa
    mRNA sequence
    • >TC010157 RA
      aaacaaaaa cgagctcgg cttgagttc ttcttaata 
      tgaatgatt ttgcctcac ctgaacggt gggttagtc 
      atcctgcga gtatccgac tcgtgttac ctgttatta 
      ttgaccaac acgtggttg gcttctgat gtcgcacaa 
      acagtacag tgtttgaag tttaaaagt gataagcaa 
      atatgaata gtgtttata atactaagg agatttaca 
      acattcggg gcgacatgt gctactcat ctgtcgcgt 
      cactgtcaa aaaaagaat cacaatgtt tgcaagaca 
      gttcatccg cttcgccag ttacgattc aacccggaa 
      aaatctaaa gctcacaaa agcctcaaa gctcatatg 
      tcaaggctt cacgggcaa gcaagcaac atttcactg 
      cgaacgtgc cctcgaata cggcactct cctcgtcgg 
      gggcgtaac tccgggcaa agggggcaa gaaacattt 
      aggcctccc tgtcttcaa cacagttaa ggaagcccg 
      agacgcctt aaaacccga cgttagcat aatctacgt 
      ccctccctt cgccgccac taaagccct acatgaagc 
      cctagaggc cgaaatccc cctaatagt gtgcataac 
      cgaaggcat accccaaca cgacatggt caagtttcg 
      ctacagatt atcccgcca agacaaatc cagattagt 
      cggcccgaa ctgccccgg aattatcgc ccctgagca 
      gtgcaaagt gggcataat gcccgcgat gatccacaa 
      aagggggtg cattgggat cgtgtccag gtccgggac 
      tctgaccta cgaggcagt gaaccaaac gaccaagca 
      tgggctggg tcagaccct ggttgtggg cattggggg 
      agatccata caatgggac gaatttcat cgactgtct 
      tgatatttt tctcaaaga tccagacac aaaaggaat 
      tattttaat tggcgaaat tggtggatg cgccgaaga 
      agaagccgc cgaattttt aaagaagaa taatacagg 
      ggataagag aaagccggt agttagtta cattgcggg 
      actttcggc acctccagg tcgacgaat ggggcacgc 
      cggtgctat tatttcggg aggaaaggg gggagcccc 
      ggagaaaat ggcggcttt gcgaaacgc gggagttat 
      tattgcaaa ctcgcccgc gttgatggg ggaccattt 
      aaaaaccga aatggataa taaaataat gatggttac 
      agcgccctc tgaaggaac gctcgacga agtcatcac 
      gaaaaatat agcaggact cgatagaaa catcaacct 
      gcagaagca aacaccgtt acaaaaact gcgcaagcg 
      tttacgtag acggatttt ggttgatac gttgatatt 
      tgtaatagt tgccttatt agtatgtct agtcgaatt 
      cggcatcat ccccaatac aaaacttgg cttcacttg 
      caaggaccc gaagctttc ctatccctt taaagggga 
      cacagtttc cgtgacaac tttactgat aagtgctta 
      tttcggacc cattcttat cataaccct agtggaagt 
      attgaaaga acactcgtt caagaaagt gcgtcttgg 
      gaccgtgtg gaactacta cagggaatg ccttatcgg 
      cgccacttt tgttcttct gataacaga agtaattaa 
      agtcatcgt gggagagcc ccgaccgca tttcttgga 
      ctcgtgtga gcccgacgt taagtgcaa agaaggcgc 
      tttcatagt cgaatacaa ctgcaccaa cacccgctt 
      gagcaactt tttcttgag cgacatcac gagatcttt 
      tccctcagg acacacttc cgtctcctt gttcatcgc 
      tctttattg ctcgtatct catccaaat tcgcattcc 
      ctctaaatc cgtaagcac tttgaccaa acccttctt 
      gatcgctgt ttgcctcac atggtgtct gttatgttc 
      cttgtcgag gataactga tcggcggca tcactggtg 
      ggatgttct gggtggggc gtttctggg actgtgtgg 
      tgcacttta cacgttgag tctgctcca tagcaagga 
      aacgaaaga gaaaaaccg cctcagcag aaccagcac 
      gagtatgac aataatgtc agagaagag caaagacac 
      tccaagtgt tgttatata atttaggga atttttata 
      cgttttcat tacttgatt acagttatt tattgtaca 
      tattaggac atttacgaa taaaatagc tacgtttag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY