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Gene information
General information
TC010155 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 13860983..13886065
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010155
    iBeetle screen iB_04770

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 2 - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection



    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 6 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Pupal morphology
    abdomen size decreased - Number of animals showing the phenotype: 6

    Comment: all pupae showed the decreased abdomen. 3 females were adult at this timepoint and 1 was still prepupa. so the number of analyzable females for this phenotype were 6.
    abdomen segment circumference decreased - Number of animals showing the phenotype: 6
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010155 RA
      MRPSAFPRK IFALFVHVV LSAQFIRVY GVITLGSEV 
      FCSRIPGLT SRQREMCRS SPDAMVAVG DGIRLATAE 
      CKYQFRHQR WNCTGIDNP TSFGHVVIV GSREAAFTY 
      AISSAGVAY AVTSACARG NISACGCAP GPKLREPAP 
      SGWKWGGCS VDINFGMRF ARKFMDARE LEGDERSVM 
      NLHNNKAGR KAVKMSLLT ECKCHGVSG SCTMKTCWK 
      TLPGFRQVG DNLMKKYYR ARPVTSVPS SPGPRGIDS 
      FRRPRKVHL VLKKGKIAI KKMPKKSDL VFLQTSPNY 
      CERDLAAGS LGTVGRSCN RTSRGNDGC DLMCCGRGY 
      NTHQYTRTW QCRCKFHWC CYVDCDTCS ERTEEYTCK
    CDS sequence
    • >TC010155 RA
      atgcgccct agtgctttt cctcggaaa atcttcgca 
      ctttttgtg catgtggtc ctttccgca cagtttatc 
      agggtttat ggtgtcatc actctcggc tccgaagtc 
      ttctgcagc cgcatcccc ggcctcacc agccgccag 
      agggagatg tgccgctcc tcgccggac gccatggtg 
      gcggtgggc gacggcata cgccttgcc accgccgag 
      tgcaagtac cagttcagg caccagcgc tggaactgc 
      accggcatc gacaacccc acgtccttc ggacacgtc 
      gtcatcgta ggctcgaga gaagctgcc ttcacatac 
      gcgatttcc agtgccgga gtggcctac gccgtcacc 
      tcggcgtgc gcccgcggc aacatatcg gcctgtggc 
      tgtgcccca ggtcccaag ctgcgggag ccagccccg 
      tctggatgg aagtggggc ggctgtagc gtcgacatt 
      aacttcggc atgaggttt gcgcgaaaa tttatggac 
      gcccgagag ctcgaaggt gatgaaaga agtgtgatg 
      aatctccac aacaacaaa gctggaaga aaggctgta 
      aaaatgagt cttttgact gagtgcaaa tgccacggc 
      gtttctggg agctgcaca atgaagact tgttggaaa 
      actcttcca ggatttaga caagtaggt gataattta 
      atgaagaag tattaccga gcgcggccg gtaacatcc 
      gtcccctcg tcgcccggt ccccgaggg atcgactcg 
      ttccggagg ccgaggaag gtgcaccta gttttaaaa 
      aagggcaag atagctatt aagaagatg ccaaaaaaa 
      tccgattta gtgttcctg cagacgtcg cccaattac 
      tgcgagcgc gatctcgct gcaggatca ttagggacg 
      gtcgggaga tcctgcaac aggacttcc cgaggaaat 
      gacggttgc gatttaatg tgctgcggc agaggatac 
      aacacgcat cagtacacc aggacgtgg caatgtaga 
      tgcaagttt cactggtgt tgctacgtc gattgtgac 
      acctgcagc gaaagaact gaggaatat acctgcaaa 
      taa
    mRNA sequence
    • >TC010155 RA
      cgaaggagc gaaaagatg cgatgggct cgagcccca 
      tgtcaatcg ttgcgttta ttacataaa atacaaata 
      atcccaaca atgtttaat gctgtcata acttcgtta 
      ataacaagt taaacaatg gtggtttac tgcatatca 
      aataaagct gtatattgc cgtcgttcg ttgaaattt 
      tagatagtg acattttga agctgggcg tggaagaca 
      gcgtttccg attttgttg aaaaatttt acttttcaa 
      tcgcaatga ttcgtcggc tgtgtgatg tcatttagg 
      attgtgaag tgatgttca gtgatgatt tgtgaaggt 
      gataagcaa atgaattgg ctgaggact cagcatgcg 
      ccctagtgc ttttcctcg gaaaatctt cgcactttt 
      tgtgcatgt ggtcctttc cgcacagtt tatcagggt 
      ttatggtgt catcactct cggctccga agtcttctg 
      cagccgcat ccccggcct caccagccg ccagaggga 
      gatgtgccg ctcctcgcc ggacgccat ggtggcggt 
      gggcgacgg catacgcct tgccaccgc cgagtgcaa 
      gtaccagtt caggcacca gcgctggaa ctgcaccgg 
      catcgacaa ccccacgtc cttcggaca cgtcgtcat 
      cgtaggctc gagagaagc tgccttcac atacgcgat 
      ttccagtgc cggagtggc ctacgccgt cacctcggc 
      gtgcgcccg cggcaacat atcggcctg tggctgtgc 
      cccaggtcc caagctgcg ggagccagc cccgtctgg 
      atggaagtg gggcggctg tagcgtcga cattaactt 
      cggcatgag gtttgcgcg aaaatttat ggacgcccg 
      agagctcga aggtgatga aagaagtgt gatgaatct 
      ccacaacaa caaagctgg aagaaaggc tgtaaaaat 
      gagtctttt gactgagtg caaatgcca cggcgtttc 
      tgggagctg cacaatgaa gacttgttg gaaaactct 
      tccaggatt tagacaagt aggtgataa tttaatgaa 
      gaagtatta ccgagcgcg gccggtaac atccgtccc 
      ctcgtcgcc cggtccccg agggatcga ctcgttccg 
      gaggccgag gaaggtgca cctagtttt aaaaaaggg 
      caagatagc tattaagaa gatgccaaa aaaatccga 
      tttagtgtt cctgcagac gtcgcccaa ttactgcga 
      gcgcgatct cgctgcagg atcattagg gacggtcgg 
      gagatcctg caacaggac ttcccgagg aaatgacgg 
      ttgcgattt aatgtgctg cggcagagg atacaacac 
      gcatcagta caccaggac gtggcaatg tagatgcaa 
      gtttcactg gtgttgcta cgtcgattg tgacacctg 
      cagcgaaag aactgagga atatacctg caaataaca 
      caaatactc gcgtttttt acgaaagta gctctttgg 
      taggacttt ttacagtaa cattcgtac ttcgacgtt 
      cacaagtta ccataatta ttaaatgtt gtttctgtt 
      tctcatgta tataacact cttcaaata ctgatcttg 
      tacaggtgt taatgtcga tttaggtgt aagaatcgt 
      agccatttt gacaatcta attgttaac tagagaact 
      ccatttgac aaactaatt taattctag attagggat 
      tagacgtat gtagtagtt ggtaaatta tttattatt 
      taaaatcca ctcttgtac cgtgctttg taaaaccgc 
      aaatacatt tttacacat ttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY