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Gene information
General information
TC010154 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 13900518..13902065
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010154
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04769

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010154 RA
      MKALIVLSA VCLCVALAA EPEKKEATQ ETKKQDKRG 
      LIGLGDHGG FGGGDLGGH EFGGGGGIE LGGGGGGFE 
      GHGGFEGGF EGHGGFEGG IGGGGGGEH EHHHHEHVK 
      TIVVEKKVP VPYTVTKHV PYTVEKKVP YEVKVPVPQ 
      PYTVEKKIP VPVKEYIKV PYIVPQPYE VIKKIPYEV 
      KVPVPRPYE VKVPVPQPY TVEKKIPVP VKVPVPQPY 
      TVEKKVPYP VKVEIPVPQ PYTVEKKVP YEVKVPVDR 
      PYPVHVPKP YPVTVEKHI PYPVEKPVP YEVKVPVDK 
      PYPVEVPKP VPYHVKVPV PQPYTVEKK VPVEIEKPV 
      PVPVKVPVD KPYPVYKEI PYPVEKPVP YPVKIPVHI 
      PVKVHHHHH EESGGGFEG SFGGGYH
    CDS sequence
    • >TC010154 RA
      atgaaggcc ttgattgta ctgagcgct gtgtgcctg 
      tgcgtggct ctagctgct gagcctgaa aagaaagag 
      gcaacgcag gagaccaag aaacaggat aaacgcggg 
      ctcatcggg ctcggcgat catggagga tttggtggc 
      ggagacttg ggtggacac gagttcgga ggcggtggc 
      ggaattgag ctcggcggc ggcggcggc ggcttcgaa 
      ggccatggg ggcttcgaa ggcggcttc gagggccac 
      ggtgggttc gagggtgga atcgggggc ggtggcggt 
      ggcgaacac gagcaccac caccacgaa cacgtcaag 
      accatagtg gtcgaaaag aaggtccca gtgccctac 
      actgtgacc aaacacgtt ccctacacc gtggaaaag 
      aaagtaccc tacgaagtc aaagtaccc gttccacaa 
      ccctatact gtggagaag aaaatcccc gtgccagtc 
      aaggagtac atcaaagta ccctacatc gtcccccag 
      ccctacgag gtgatcaag aagatcccc tacgaagtc 
      aaagttcca gtgccaagg ccctacgaa gtcaaagtc 
      cccgttccc caaccctac accgtcgaa aagaaaatc 
      ccagttcca gtcaaagtg cccgtacca cagccctat 
      accgtcgaa aagaaagtt ccgtatcca gtcaaggta 
      gaaatccca gtcccacaa ccctacacc gtggagaaa 
      aaggtccct tacgaagtt aaggttcct gtggatagg 
      ccctacccc gtgcacgta cccaaaccc tacccagtc 
      actgtagaa aagcacatt ccgtaccca gtcgaaaaa 
      ccagtgcct tacgaggtt aaggtacca gtcgacaag 
      ccctacccc gtcgaagta cccaagccg gtaccatac 
      cacgtcaaa gtacccgtt cctcaaccc tacactgtt 
      gagaagaag gtgccagta gaaatcgag aaacccgta 
      cccgtaccc gtcaaggta cctgtagat aaaccatac 
      cccgtatac aaagaaatt ccatacccc gtagaaaaa 
      ccagtaccc tatcctgta aaaatcccc gtgcacata 
      cccgttaag gtccaccac caccaccac gaagagtct 
      ggtggcggt tttgaagga tctttcggc ggagggtac 
      cactag
    mRNA sequence
    • >TC010154 RA
      agccttcca catctccaa tcatgaagg ccttgattg 
      tactgagcg ctgtgtgcc tgtgcgtgg ctctagctg 
      ctgagcctg aaaagaaag aggcaacgc aggagacca 
      agaaacagg ataaacgcg ggctcatcg ggctcggcg 
      atcatggag gatttggtg gcggagact tgggtggac 
      acgagttcg gaggcggtg gcggaattg agctcggcg 
      gcggcggcg gcggcttcg aaggccatg ggggcttcg 
      aaggcggct tcgagggcc acggtgggt tcgagggtg 
      gaatcgggg gcggtggcg gtggcgaac acgagcacc 
      accaccacg aacacgtca agaccatag tggtcgaaa 
      agaaggtcc cagtgccct acactgtga ccaaacacg 
      ttccctaca ccgtggaaa agaaagtac cctacgaag 
      tcaaagtac ccgttccac aaccctata ctgtggaga 
      agaaaatcc ccgtgccag tcaaggagt acatcaaag 
      taccctaca tcgtccccc agccctacg aggtgatca 
      agaagatcc cctacgaag tcaaagttc cagtgccaa 
      ggccctacg aagtcaaag tccccgttc cccaaccct 
      acaccgtcg aaaagaaaa tcccagttc cagtcaaag 
      tgcccgtac cacagccct ataccgtcg aaaagaaag 
      ttccgtatc cagtcaagg tagaaatcc cagtcccac 
      aaccctaca ccgtggaga aaaaggtcc cttacgaag 
      ttaaggttc ctgtggata ggccctacc ccgtgcacg 
      tacccaaac cctacccag tcactgtag aaaagcaca 
      ttccgtacc cagtcgaaa aaccagtgc cttacgagg 
      ttaaggtac cagtcgaca agccctacc ccgtcgaag 
      tacccaagc cggtaccat accacgtca aagtacccg 
      ttcctcaac cctacactg ttgagaaga aggtgccag 
      tagaaatcg agaaacccg tacccgtac ccgtcaagg 
      tacctgtag ataaaccat accccgtat acaaagaaa 
      ttccatacc ccgtagaaa aaccagtac cctatcctg 
      taaaaatcc ccgtgcaca tacccgtta aggtccacc 
      accaccacc acgaagagt ctggtggcg gttttgaag 
      gatctttcg gcggagggt accactagt tgttgttag 
      ctcgttatt aattgttat gtaattgct atttttata 
      cttgtgctt gttaaattt cgtttcgac atttgctca 
      aattgaatc atttcctca ttaaattca taaagcgtg 
      cagtattat tacttagaa actgtgtta attagtata 
      atttatcta cttttataa taaatcatt tctacgtac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY