Status
Please wait ...
Gene information
General information
TC010150 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 13951903..13955041
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010150
    iBeetle screen iB_04768

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    thorax pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    thorax pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 6 (16 dpi)
    Days post injection


    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010150 RA
      MSSIEERLQ IKREPLDKW SIREQLALA VARNMDQNL 
      TSVSRALKP FGEPNRPPD WFHQKNCAA QYGALLANV 
      ETPKRKKRT SGGEVTIET PAEVILRKL LAERQAELK 
      RLLAEEKAE YQKLQRDKA LLQSGNVSE EQLDIWCKE 
      IDEEEHRKE QEALAHEQW LKERELRKQ EIERAWRPV 
      KPSPVIGQK RKNSEAMDT IPEMENTTE EQKILVQEP 
      PKPALSPLL TSLLKSPSQ VPNVTTSSI LHSAITNQR 
      PTNTTTSPM IASLLSSST GETVSPGLQ LLVSTAIGQ 
      EPAGEETQQ QNVNTNDIL DDANLQILK IDDLASSIL 
      IQNGPLPEI KNDVDVIIS DLIENAQDI VADPEQHLQ 
      LDGNGDLNL NLELEDFEE SETDMKDET KETEKRTET 
      TTPAIDPFE FQEDPEVFE PAKPATAVK TDASKTVVP 
      QDSDDRPQT TEEAPNSDN KHLTSNGEE SRDEVVEVK 
      NEPTDDLDK KDKSTTPGS VEIVEVVVM DDEDTDKEV 
      NKTEDPAHS VKAEPKEET AGESTEVPE IKSETDAKV 
      SENEKTIVE IEKKDDRKL DESTTLTSD YNEELYDDI 
      NMEVKVDKT GKAKRDYSR TKKKDDKEF DVLLAIEKA 
      QRDESELVE ESFSDRDYS ELDRKDSKS RIKSENERS 
      NSPWTEEED VSTIRTRRR LSTPATPID SVPGSPVSS 
      IYFYDDDRE YRNWKKSIM LVYSRLAAN KYASLFSKP 
      ITDDQAPGY HSVVYRPMD LLTIRKNIE NGAIRTTQE 
      FQRDVLLML NNAIMYNKT NDTVYNMAR QMQQESMQQ 
      IQILLQAQS QDLPVRRET RTSEPGCKR KRGNEENSR 
      SKKRKDD
    CDS sequence
    • >TC010150 RA
      atgagttca attgaagaa cgtttacaa ataaagcgt 
      gaaccgctg gataaatgg tcaatccga gaacagttg 
      gctctagca gtggctcgt aacatggac cagaattta 
      acaagtgtg agccgagct ttaaaaccc tttggtgaa 
      cccaacaga ccccctgat tggttccac cagaagaac 
      tgtgcagcc cagtatggg gctctttta gcgaatgtt 
      gaaacacct aagaggaag aaaagaacg tctgggggc 
      gaagtcact atcgaaacc cccgccgaa gtcattcta 
      cgcaaactt ttggccgag agacaagct gagctcaag 
      agactacta gcggaagaa aaagcagaa tatcagaaa 
      ttgcagaga gataaagct ctgcttcag tcaggtaac 
      gtaagtgaa gaacaactg gatatttgg tgtaaggaa 
      atagatgaa gaggaacac aggaaggaa caagaagcc 
      ctggcacac gaacagtgg ctaaaggag agggagtta 
      cgaaaacag gaaatagaa agagcgtgg agaccggtt 
      aagccatct ccggtgatt ggtcaaaaa cgtaaaaat 
      tccgaagct atggacaca atcccagag atggaaaat 
      acaacagaa gaacagaaa atcctagtg caggagccc 
      cctaaaccg gccctgtcg ccacttctg acaagtctt 
      ctgaaatca ccatcacaa gttccaaac gttacaact 
      tcctcaata ttgcattca gcgattacc aaccaacgg 
      ccaaccaac acaactact agccccatg attgccagt 
      cttttaagc tcctcaact ggagaaaca gtttctcca 
      ggattgcaa cttttggtc agtacagct ataggacaa 
      gagccagcc ggagaagaa acccaacaa cagaatgtc 
      aatactaac gatatcctc gatgatgcc aatctacaa 
      attttgaaa attgatgac ttggcgagc agtatcttg 
      atccagaat ggcccactt ccagaaata aagaatgat 
      gttgatgtt atcatctcc gatttgatt gagaacgcg 
      caggatata gttgcagat ccggaacag catctacaa 
      ctggacgga aacggtgat cttaatcta aatttggaa 
      ctggaagat ttcgaagag agtgaaact gatatgaag 
      gacgaaacc aaagaaaca gaaaaacgg acggaaact 
      acaacaccg gcaatagat ccgtttgag ttccaagaa 
      gatccggaa gtttttgaa ccagctaaa cctgcaaca 
      gccgtgaaa acagacgct tctaaaact gtggtacca 
      caggacagc gacgatcgg ccacaaact actgaagaa 
      gccccaaat tcagacaat aaacacttg acttcaaat 
      ggcgaagaa tctcgggat gaagtggta gaagtaaaa 
      aacgaacca actgatgat ctcgataag aaagataaa 
      agcacaact cctggatcc gtcgaaatt gtagaagtt 
      gtagttatg gatgatgaa gacactgac aaggaagtc 
      aataaaact gaagatcca gcccattcc gttaaagcg 
      gagccaaag gaagaaaca gcaggagaa agtacagaa 
      gttccggaa ataaaatca gaaacagac gccaaagtt 
      tcagaaaat gaaaaaact attgtagag attgagaag 
      aaagacgat aggaaactg gacgagtca accacattg 
      acctccgac tataacgag gaattgtac gacgatatc 
      aatatggaa gtgaaagtg gacaagacc gggaaagca 
      aagcgcgat tattccagg acgaagaag aaggacgat 
      aaagaattt gatgttctc ttggccatt gaaaaggcg 
      caaagagac gaaagtgag ttggttgaa gagagtttc 
      tctgaccgg gattacagc gaactggac cgtaaagac 
      agcaaatca cggattaag agcgagaat gaaagatct 
      aacagtccc tggacggag gaggaagat gtgtctaca 
      atacggaca aggaggcga ctttcaact cctgcgact 
      ccgattgat tcagttcca ggatcacca gtttccagt 
      atatatttt tacgatgat gatcgagag tatcgcaat 
      tggaagaag tctataatg cttgtgtat agtagattg 
      gctgcaaat aaatacgcg tcactgttt tctaaacca 
      atcaccgat gaccaagct ccagggtat cacagtgtg 
      gtgtataga ccgatggat ttgttgacg attagaaag 
      aatatagag aatggtgcg ataagaaca acgcaggag 
      tttcaaaga gacgtcctt cttatgctt aataatgca 
      attatgtat aataaaaca aatgatacg gtttataat 
      atggcgaga caaatgcaa caagagtcg atgcaacaa 
      atacagatt ctgttgcaa gcgcaatcg caggatttg 
      cctgttagg agggaaact aggactagt gagccaggg 
      tgtaagagg aagaggggt aatgaggag aattccagg 
      agtaagaaa aggaaggat gattag
    mRNA sequence
    • >TC010150 RA
      cattacatc atcttacag tactgattg tggttgact 
      ttctttaaa aatcaggca gaacttctg catagtttg 
      accaggttt gacactttg acagttgac aaatataaa 
      caacgttca agcttgtta taacgataa ttcttgtgt 
      gcgaatttc tacttcaag aatgtatat tcagtgata 
      ctattagtc actttcctg gcgaggttc ttgtatttg 
      atttttggg attttggga caacataac ctcaaaatg 
      agttcaatt gaagaacgt ttacaaata aagcgtgaa 
      ccgctggat aaatggtca atccgagaa cagttggct 
      ctagcagtg gctcgtaac atggaccag aatttaaca 
      agtgtgagc cgagcttta aaacccttt ggtgaaccc 
      aacagaccc cctgattgg ttccaccag aagaactgt 
      gcagcccag tatggggct cttttagcg aatgttgaa 
      acacctaag aggaagaaa agaacgtct gggggcgaa 
      gtcactatc gaaaccccc gccgaagtc attctacgc 
      aaacttttg gccgagaga caagctgag ctcaagaga 
      ctactagcg gaagaaaaa gcagaatat cagaaattg 
      cagagagat aaagctctg cttcagtca ggtaacgta 
      agtgaagaa caactggat atttggtgt aaggaaata 
      gatgaagag gaacacagg aaggaacaa gaagccctg 
      gcacacgaa cagtggcta aaggagagg gagttacga 
      aaacaggaa atagaaaga gcgtggaga ccggttaag 
      ccatctccg gtgattggt caaaaacgt aaaaattcc 
      gaagctatg gacacaatc ccagagatg gaaaataca 
      acagaagaa cagaaaatc ctagtgcag gagccccct 
      aaaccggcc ctgtcgcca cttctgaca agtcttctg 
      aaatcacca tcacaagtt ccaaacgtt acaacttcc 
      tcaatattg cattcagcg attaccaac caacggcca 
      accaacaca actactagc cccatgatt gccagtctt 
      ttaagctcc tcaactgga gaaacagtt tctccagga 
      ttgcaactt ttggtcagt acagctata ggacaagag 
      ccagccgga gaagaaacc caacaacag aatgtcaat 
      actaacgat atcctcgat gatgccaat ctacaaatt 
      ttgaaaatt gatgacttg gcgagcagt atcttgatc 
      cagaatggc ccacttcca gaaataaag aatgatgtt 
      gatgttatc atctccgat ttgattgag aacgcgcag 
      gatatagtt gcagatccg gaacagcat ctacaactg 
      gacggaaac ggtgatctt aatctaaat ttggaactg 
      gaagatttc gaagagagt gaaactgat atgaaggac 
      gaaaccaaa gaaacagaa aaacggacg gaaactaca 
      acaccggca atagatccg tttgagttc caagaagat 
      ccggaagtt tttgaacca gctaaacct gcaacagcc 
      gtgaaaaca gacgcttct aaaactgtg gtaccacag 
      gacagcgac gatcggcca caaactact gaagaagcc 
      ccaaattca gacaataaa cacttgact tcaaatggc 
      gaagaatct cgggatgaa gtggtagaa gtaaaaaac 
      gaaccaact gatgatctc gataagaaa gataaaagc 
      acaactcct ggatccgtc gaaattgta gaagttgta 
      gttatggat gatgaagac actgacaag gaagtcaat 
      aaaactgaa gatccagcc cattccgtt aaagcggag 
      ccaaaggaa gaaacagca ggagaaagt acagaagtt 
      ccggaaata aaatcagaa acagacgcc aaagtttca 
      gaaaatgaa aaaactatt gtagagatt gagaagaaa 
      gacgatagg aaactggac gagtcaacc acattgacc 
      tccgactat aacgaggaa ttgtacgac gatatcaat 
      atggaagtg aaagtggac aagaccggg aaagcaaag 
      cgcgattat tccaggacg aagaagaag gacgataaa 
      gaatttgat gttctcttg gccattgaa aaggcgcaa 
      agagacgaa agtgagttg gttgaagag agtttctct 
      gaccgggat tacagcgaa ctggaccgt aaagacagc 
      aaatcacgg attaagagc gagaatgaa agatctaac 
      agtccctgg acggaggag gaagatgtg tctacaata 
      cggacaagg aggcgactt tcaactcct gcgactccg 
      attgattca gttccagga tcaccagtt tccagtata 
      tatttttac gatgatgat cgagagtat cgcaattgg 
      aagaagtct ataatgctt gtgtatagt agattggct 
      gcaaataaa tacgcgtca ctgttttct aaaccaatc 
      accgatgac caagctcca gggtatcac agtgtggtg 
      tatagaccg atggatttg ttgacgatt agaaagaat 
      atagagaat ggtgcgata agaacaacg caggagttt 
      caaagagac gtccttctt atgcttaat aatgcaatt 
      atgtataat aaaacaaat gatacggtt tataatatg 
      gcgagacaa atgcaacaa gagtcgatg caacaaata 
      cagattctg ttgcaagcg caatcgcag gatttgcct 
      gttaggagg gaaactagg actagtgag ccagggtgt 
      aagaggaag aggggtaat gaggagaat tccaggagt 
      aagaaaagg aaggatgat tagcattac gttttgcgg 
      tattcggat aagctgttt gattttagt gattttttc 
      tagtattag ttttattca tattgatat aaatatgaa 
      caattttat gctcgtatt gttagaatt ttcgaatac 
      aaatactga tgtacagtt ctttgtagc tcgtgatca 
      aatacatta atatccgtg tttaataaa tgtaagcat 
      atctactta ggatttctt atgtaagca tcgttgtgt 
      aaatatttt tatattatg taatataaa atacctata 
      act
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY