General information | |||
TC010150 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 13951903..13955041 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC010150 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_04768 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
thorax pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
thorax pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
abdomen pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
abdomen pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 6 (16 dpi)
- >TC010150 RA
MSSIEERLQ IKREPLDKW SIREQLALA VARNMDQNL TSVSRALKP FGEPNRPPD WFHQKNCAA QYGALLANV ETPKRKKRT SGGEVTIET PAEVILRKL LAERQAELK RLLAEEKAE YQKLQRDKA LLQSGNVSE EQLDIWCKE IDEEEHRKE QEALAHEQW LKERELRKQ EIERAWRPV KPSPVIGQK RKNSEAMDT IPEMENTTE EQKILVQEP PKPALSPLL TSLLKSPSQ VPNVTTSSI LHSAITNQR PTNTTTSPM IASLLSSST GETVSPGLQ LLVSTAIGQ EPAGEETQQ QNVNTNDIL DDANLQILK IDDLASSIL IQNGPLPEI KNDVDVIIS DLIENAQDI VADPEQHLQ LDGNGDLNL NLELEDFEE SETDMKDET KETEKRTET TTPAIDPFE FQEDPEVFE PAKPATAVK TDASKTVVP QDSDDRPQT TEEAPNSDN KHLTSNGEE SRDEVVEVK NEPTDDLDK KDKSTTPGS VEIVEVVVM DDEDTDKEV NKTEDPAHS VKAEPKEET AGESTEVPE IKSETDAKV SENEKTIVE IEKKDDRKL DESTTLTSD YNEELYDDI NMEVKVDKT GKAKRDYSR TKKKDDKEF DVLLAIEKA QRDESELVE ESFSDRDYS ELDRKDSKS RIKSENERS NSPWTEEED VSTIRTRRR LSTPATPID SVPGSPVSS IYFYDDDRE YRNWKKSIM LVYSRLAAN KYASLFSKP ITDDQAPGY HSVVYRPMD LLTIRKNIE NGAIRTTQE FQRDVLLML NNAIMYNKT NDTVYNMAR QMQQESMQQ IQILLQAQS QDLPVRRET RTSEPGCKR KRGNEENSR SKKRKDD
- >TC010150 RA
atgagttca attgaagaa cgtttacaa ataaagcgt gaaccgctg gataaatgg tcaatccga gaacagttg gctctagca gtggctcgt aacatggac cagaattta acaagtgtg agccgagct ttaaaaccc tttggtgaa cccaacaga ccccctgat tggttccac cagaagaac tgtgcagcc cagtatggg gctctttta gcgaatgtt gaaacacct aagaggaag aaaagaacg tctgggggc gaagtcact atcgaaacc cccgccgaa gtcattcta cgcaaactt ttggccgag agacaagct gagctcaag agactacta gcggaagaa aaagcagaa tatcagaaa ttgcagaga gataaagct ctgcttcag tcaggtaac gtaagtgaa gaacaactg gatatttgg tgtaaggaa atagatgaa gaggaacac aggaaggaa caagaagcc ctggcacac gaacagtgg ctaaaggag agggagtta cgaaaacag gaaatagaa agagcgtgg agaccggtt aagccatct ccggtgatt ggtcaaaaa cgtaaaaat tccgaagct atggacaca atcccagag atggaaaat acaacagaa gaacagaaa atcctagtg caggagccc cctaaaccg gccctgtcg ccacttctg acaagtctt ctgaaatca ccatcacaa gttccaaac gttacaact tcctcaata ttgcattca gcgattacc aaccaacgg ccaaccaac acaactact agccccatg attgccagt cttttaagc tcctcaact ggagaaaca gtttctcca ggattgcaa cttttggtc agtacagct ataggacaa gagccagcc ggagaagaa acccaacaa cagaatgtc aatactaac gatatcctc gatgatgcc aatctacaa attttgaaa attgatgac ttggcgagc agtatcttg atccagaat ggcccactt ccagaaata aagaatgat gttgatgtt atcatctcc gatttgatt gagaacgcg caggatata gttgcagat ccggaacag catctacaa ctggacgga aacggtgat cttaatcta aatttggaa ctggaagat ttcgaagag agtgaaact gatatgaag gacgaaacc aaagaaaca gaaaaacgg acggaaact acaacaccg gcaatagat ccgtttgag ttccaagaa gatccggaa gtttttgaa ccagctaaa cctgcaaca gccgtgaaa acagacgct tctaaaact gtggtacca caggacagc gacgatcgg ccacaaact actgaagaa gccccaaat tcagacaat aaacacttg acttcaaat ggcgaagaa tctcgggat gaagtggta gaagtaaaa aacgaacca actgatgat ctcgataag aaagataaa agcacaact cctggatcc gtcgaaatt gtagaagtt gtagttatg gatgatgaa gacactgac aaggaagtc aataaaact gaagatcca gcccattcc gttaaagcg gagccaaag gaagaaaca gcaggagaa agtacagaa gttccggaa ataaaatca gaaacagac gccaaagtt tcagaaaat gaaaaaact attgtagag attgagaag aaagacgat aggaaactg gacgagtca accacattg acctccgac tataacgag gaattgtac gacgatatc aatatggaa gtgaaagtg gacaagacc gggaaagca aagcgcgat tattccagg acgaagaag aaggacgat aaagaattt gatgttctc ttggccatt gaaaaggcg caaagagac gaaagtgag ttggttgaa gagagtttc tctgaccgg gattacagc gaactggac cgtaaagac agcaaatca cggattaag agcgagaat gaaagatct aacagtccc tggacggag gaggaagat gtgtctaca atacggaca aggaggcga ctttcaact cctgcgact ccgattgat tcagttcca ggatcacca gtttccagt atatatttt tacgatgat gatcgagag tatcgcaat tggaagaag tctataatg cttgtgtat agtagattg gctgcaaat aaatacgcg tcactgttt tctaaacca atcaccgat gaccaagct ccagggtat cacagtgtg gtgtataga ccgatggat ttgttgacg attagaaag aatatagag aatggtgcg ataagaaca acgcaggag tttcaaaga gacgtcctt cttatgctt aataatgca attatgtat aataaaaca aatgatacg gtttataat atggcgaga caaatgcaa caagagtcg atgcaacaa atacagatt ctgttgcaa gcgcaatcg caggatttg cctgttagg agggaaact aggactagt gagccaggg tgtaagagg aagaggggt aatgaggag aattccagg agtaagaaa aggaaggat gattag
- >TC010150 RA
cattacatc atcttacag tactgattg tggttgact ttctttaaa aatcaggca gaacttctg catagtttg accaggttt gacactttg acagttgac aaatataaa caacgttca agcttgtta taacgataa ttcttgtgt gcgaatttc tacttcaag aatgtatat tcagtgata ctattagtc actttcctg gcgaggttc ttgtatttg atttttggg attttggga caacataac ctcaaaatg agttcaatt gaagaacgt ttacaaata aagcgtgaa ccgctggat aaatggtca atccgagaa cagttggct ctagcagtg gctcgtaac atggaccag aatttaaca agtgtgagc cgagcttta aaacccttt ggtgaaccc aacagaccc cctgattgg ttccaccag aagaactgt gcagcccag tatggggct cttttagcg aatgttgaa acacctaag aggaagaaa agaacgtct gggggcgaa gtcactatc gaaaccccc gccgaagtc attctacgc aaacttttg gccgagaga caagctgag ctcaagaga ctactagcg gaagaaaaa gcagaatat cagaaattg cagagagat aaagctctg cttcagtca ggtaacgta agtgaagaa caactggat atttggtgt aaggaaata gatgaagag gaacacagg aaggaacaa gaagccctg gcacacgaa cagtggcta aaggagagg gagttacga aaacaggaa atagaaaga gcgtggaga ccggttaag ccatctccg gtgattggt caaaaacgt aaaaattcc gaagctatg gacacaatc ccagagatg gaaaataca acagaagaa cagaaaatc ctagtgcag gagccccct aaaccggcc ctgtcgcca cttctgaca agtcttctg aaatcacca tcacaagtt ccaaacgtt acaacttcc tcaatattg cattcagcg attaccaac caacggcca accaacaca actactagc cccatgatt gccagtctt ttaagctcc tcaactgga gaaacagtt tctccagga ttgcaactt ttggtcagt acagctata ggacaagag ccagccgga gaagaaacc caacaacag aatgtcaat actaacgat atcctcgat gatgccaat ctacaaatt ttgaaaatt gatgacttg gcgagcagt atcttgatc cagaatggc ccacttcca gaaataaag aatgatgtt gatgttatc atctccgat ttgattgag aacgcgcag gatatagtt gcagatccg gaacagcat ctacaactg gacggaaac ggtgatctt aatctaaat ttggaactg gaagatttc gaagagagt gaaactgat atgaaggac gaaaccaaa gaaacagaa aaacggacg gaaactaca acaccggca atagatccg tttgagttc caagaagat ccggaagtt tttgaacca gctaaacct gcaacagcc gtgaaaaca gacgcttct aaaactgtg gtaccacag gacagcgac gatcggcca caaactact gaagaagcc ccaaattca gacaataaa cacttgact tcaaatggc gaagaatct cgggatgaa gtggtagaa gtaaaaaac gaaccaact gatgatctc gataagaaa gataaaagc acaactcct ggatccgtc gaaattgta gaagttgta gttatggat gatgaagac actgacaag gaagtcaat aaaactgaa gatccagcc cattccgtt aaagcggag ccaaaggaa gaaacagca ggagaaagt acagaagtt ccggaaata aaatcagaa acagacgcc aaagtttca gaaaatgaa aaaactatt gtagagatt gagaagaaa gacgatagg aaactggac gagtcaacc acattgacc tccgactat aacgaggaa ttgtacgac gatatcaat atggaagtg aaagtggac aagaccggg aaagcaaag cgcgattat tccaggacg aagaagaag gacgataaa gaatttgat gttctcttg gccattgaa aaggcgcaa agagacgaa agtgagttg gttgaagag agtttctct gaccgggat tacagcgaa ctggaccgt aaagacagc aaatcacgg attaagagc gagaatgaa agatctaac agtccctgg acggaggag gaagatgtg tctacaata cggacaagg aggcgactt tcaactcct gcgactccg attgattca gttccagga tcaccagtt tccagtata tatttttac gatgatgat cgagagtat cgcaattgg aagaagtct ataatgctt gtgtatagt agattggct gcaaataaa tacgcgtca ctgttttct aaaccaatc accgatgac caagctcca gggtatcac agtgtggtg tatagaccg atggatttg ttgacgatt agaaagaat atagagaat ggtgcgata agaacaacg caggagttt caaagagac gtccttctt atgcttaat aatgcaatt atgtataat aaaacaaat gatacggtt tataatatg gcgagacaa atgcaacaa gagtcgatg caacaaata cagattctg ttgcaagcg caatcgcag gatttgcct gttaggagg gaaactagg actagtgag ccagggtgt aagaggaag aggggtaat gaggagaat tccaggagt aagaaaagg aaggatgat tagcattac gttttgcgg tattcggat aagctgttt gattttagt gattttttc tagtattag ttttattca tattgatat aaatatgaa caattttat gctcgtatt gttagaatt ttcgaatac aaatactga tgtacagtt ctttgtagc tcgtgatca aatacatta atatccgtg tttaataaa tgtaagcat atctactta ggatttctt atgtaagca tcgttgtgt aaatatttt tatattatg taatataaa atacctata act
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
