Status
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Gene information
General information
TC010150 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 13951903..13955041
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC010150
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04768

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     thorax pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    thorax pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 6 (16 dpi)
    Days post injection

    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010150 RA
      MSSIEERLQ IKREPLDKW SIREQLALA VARNMDQNL 
TSVSRALKP FGEPNRPPD WFHQKNCAA QYGALLANV 
ETPKRKKRT SGGEVTIET PAEVILRKL LAERQAELK 
RLLAEEKAE YQKLQRDKA LLQSGNVSE EQLDIWCKE 
IDEEEHRKE QEALAHEQW LKERELRKQ EIERAWRPV 
KPSPVIGQK RKNSEAMDT IPEMENTTE EQKILVQEP 
PKPALSPLL TSLLKSPSQ VPNVTTSSI LHSAITNQR 
PTNTTTSPM IASLLSSST GETVSPGLQ LLVSTAIGQ 
EPAGEETQQ QNVNTNDIL DDANLQILK IDDLASSIL 
IQNGPLPEI KNDVDVIIS DLIENAQDI VADPEQHLQ 
LDGNGDLNL NLELEDFEE SETDMKDET KETEKRTET 
TTPAIDPFE FQEDPEVFE PAKPATAVK TDASKTVVP 
QDSDDRPQT TEEAPNSDN KHLTSNGEE SRDEVVEVK 
NEPTDDLDK KDKSTTPGS VEIVEVVVM DDEDTDKEV 
NKTEDPAHS VKAEPKEET AGESTEVPE IKSETDAKV 
SENEKTIVE IEKKDDRKL DESTTLTSD YNEELYDDI 
NMEVKVDKT GKAKRDYSR TKKKDDKEF DVLLAIEKA 
QRDESELVE ESFSDRDYS ELDRKDSKS RIKSENERS 
NSPWTEEED VSTIRTRRR LSTPATPID SVPGSPVSS 
IYFYDDDRE YRNWKKSIM LVYSRLAAN KYASLFSKP 
ITDDQAPGY HSVVYRPMD LLTIRKNIE NGAIRTTQE 
FQRDVLLML NNAIMYNKT NDTVYNMAR QMQQESMQQ 
IQILLQAQS QDLPVRRET RTSEPGCKR KRGNEENSR 
SKKRKDD
    CDS sequence
    • >TC010150 RA
      atgagttca attgaagaa cgtttacaa ataaagcgt 
gaaccgctg gataaatgg tcaatccga gaacagttg 
gctctagca gtggctcgt aacatggac cagaattta 
acaagtgtg agccgagct ttaaaaccc tttggtgaa 
cccaacaga ccccctgat tggttccac cagaagaac 
tgtgcagcc cagtatggg gctctttta gcgaatgtt 
gaaacacct aagaggaag aaaagaacg tctgggggc 
gaagtcact atcgaaacc cccgccgaa gtcattcta 
cgcaaactt ttggccgag agacaagct gagctcaag 
agactacta gcggaagaa aaagcagaa tatcagaaa 
ttgcagaga gataaagct ctgcttcag tcaggtaac 
gtaagtgaa gaacaactg gatatttgg tgtaaggaa 
atagatgaa gaggaacac aggaaggaa caagaagcc 
ctggcacac gaacagtgg ctaaaggag agggagtta 
cgaaaacag gaaatagaa agagcgtgg agaccggtt 
aagccatct ccggtgatt ggtcaaaaa cgtaaaaat 
tccgaagct atggacaca atcccagag atggaaaat 
acaacagaa gaacagaaa atcctagtg caggagccc 
cctaaaccg gccctgtcg ccacttctg acaagtctt 
ctgaaatca ccatcacaa gttccaaac gttacaact 
tcctcaata ttgcattca gcgattacc aaccaacgg 
ccaaccaac acaactact agccccatg attgccagt 
cttttaagc tcctcaact ggagaaaca gtttctcca 
ggattgcaa cttttggtc agtacagct ataggacaa 
gagccagcc ggagaagaa acccaacaa cagaatgtc 
aatactaac gatatcctc gatgatgcc aatctacaa 
attttgaaa attgatgac ttggcgagc agtatcttg 
atccagaat ggcccactt ccagaaata aagaatgat 
gttgatgtt atcatctcc gatttgatt gagaacgcg 
caggatata gttgcagat ccggaacag catctacaa 
ctggacgga aacggtgat cttaatcta aatttggaa 
ctggaagat ttcgaagag agtgaaact gatatgaag 
gacgaaacc aaagaaaca gaaaaacgg acggaaact 
acaacaccg gcaatagat ccgtttgag ttccaagaa 
gatccggaa gtttttgaa ccagctaaa cctgcaaca 
gccgtgaaa acagacgct tctaaaact gtggtacca 
caggacagc gacgatcgg ccacaaact actgaagaa 
gccccaaat tcagacaat aaacacttg acttcaaat 
ggcgaagaa tctcgggat gaagtggta gaagtaaaa 
aacgaacca actgatgat ctcgataag aaagataaa 
agcacaact cctggatcc gtcgaaatt gtagaagtt 
gtagttatg gatgatgaa gacactgac aaggaagtc 
aataaaact gaagatcca gcccattcc gttaaagcg 
gagccaaag gaagaaaca gcaggagaa agtacagaa 
gttccggaa ataaaatca gaaacagac gccaaagtt 
tcagaaaat gaaaaaact attgtagag attgagaag 
aaagacgat aggaaactg gacgagtca accacattg 
acctccgac tataacgag gaattgtac gacgatatc 
aatatggaa gtgaaagtg gacaagacc gggaaagca 
aagcgcgat tattccagg acgaagaag aaggacgat 
aaagaattt gatgttctc ttggccatt gaaaaggcg 
caaagagac gaaagtgag ttggttgaa gagagtttc 
tctgaccgg gattacagc gaactggac cgtaaagac 
agcaaatca cggattaag agcgagaat gaaagatct 
aacagtccc tggacggag gaggaagat gtgtctaca 
atacggaca aggaggcga ctttcaact cctgcgact 
ccgattgat tcagttcca ggatcacca gtttccagt 
atatatttt tacgatgat gatcgagag tatcgcaat 
tggaagaag tctataatg cttgtgtat agtagattg 
gctgcaaat aaatacgcg tcactgttt tctaaacca 
atcaccgat gaccaagct ccagggtat cacagtgtg 
gtgtataga ccgatggat ttgttgacg attagaaag 
aatatagag aatggtgcg ataagaaca acgcaggag 
tttcaaaga gacgtcctt cttatgctt aataatgca 
attatgtat aataaaaca aatgatacg gtttataat 
atggcgaga caaatgcaa caagagtcg atgcaacaa 
atacagatt ctgttgcaa gcgcaatcg caggatttg 
cctgttagg agggaaact aggactagt gagccaggg 
tgtaagagg aagaggggt aatgaggag aattccagg 
agtaagaaa aggaaggat gattag
    mRNA sequence
    • >TC010150 RA
      cattacatc atcttacag tactgattg tggttgact 
ttctttaaa aatcaggca gaacttctg catagtttg 
accaggttt gacactttg acagttgac aaatataaa 
caacgttca agcttgtta taacgataa ttcttgtgt 
gcgaatttc tacttcaag aatgtatat tcagtgata 
ctattagtc actttcctg gcgaggttc ttgtatttg 
atttttggg attttggga caacataac ctcaaaatg 
agttcaatt gaagaacgt ttacaaata aagcgtgaa 
ccgctggat aaatggtca atccgagaa cagttggct 
ctagcagtg gctcgtaac atggaccag aatttaaca 
agtgtgagc cgagcttta aaacccttt ggtgaaccc 
aacagaccc cctgattgg ttccaccag aagaactgt 
gcagcccag tatggggct cttttagcg aatgttgaa 
acacctaag aggaagaaa agaacgtct gggggcgaa 
gtcactatc gaaaccccc gccgaagtc attctacgc 
aaacttttg gccgagaga caagctgag ctcaagaga 
ctactagcg gaagaaaaa gcagaatat cagaaattg 
cagagagat aaagctctg cttcagtca ggtaacgta 
agtgaagaa caactggat atttggtgt aaggaaata 
gatgaagag gaacacagg aaggaacaa gaagccctg 
gcacacgaa cagtggcta aaggagagg gagttacga 
aaacaggaa atagaaaga gcgtggaga ccggttaag 
ccatctccg gtgattggt caaaaacgt aaaaattcc 
gaagctatg gacacaatc ccagagatg gaaaataca 
acagaagaa cagaaaatc ctagtgcag gagccccct 
aaaccggcc ctgtcgcca cttctgaca agtcttctg 
aaatcacca tcacaagtt ccaaacgtt acaacttcc 
tcaatattg cattcagcg attaccaac caacggcca 
accaacaca actactagc cccatgatt gccagtctt 
ttaagctcc tcaactgga gaaacagtt tctccagga 
ttgcaactt ttggtcagt acagctata ggacaagag 
ccagccgga gaagaaacc caacaacag aatgtcaat 
actaacgat atcctcgat gatgccaat ctacaaatt 
ttgaaaatt gatgacttg gcgagcagt atcttgatc 
cagaatggc ccacttcca gaaataaag aatgatgtt 
gatgttatc atctccgat ttgattgag aacgcgcag 
gatatagtt gcagatccg gaacagcat ctacaactg 
gacggaaac ggtgatctt aatctaaat ttggaactg 
gaagatttc gaagagagt gaaactgat atgaaggac 
gaaaccaaa gaaacagaa aaacggacg gaaactaca 
acaccggca atagatccg tttgagttc caagaagat 
ccggaagtt tttgaacca gctaaacct gcaacagcc 
gtgaaaaca gacgcttct aaaactgtg gtaccacag 
gacagcgac gatcggcca caaactact gaagaagcc 
ccaaattca gacaataaa cacttgact tcaaatggc 
gaagaatct cgggatgaa gtggtagaa gtaaaaaac 
gaaccaact gatgatctc gataagaaa gataaaagc 
acaactcct ggatccgtc gaaattgta gaagttgta 
gttatggat gatgaagac actgacaag gaagtcaat 
aaaactgaa gatccagcc cattccgtt aaagcggag 
ccaaaggaa gaaacagca ggagaaagt acagaagtt 
ccggaaata aaatcagaa acagacgcc aaagtttca 
gaaaatgaa aaaactatt gtagagatt gagaagaaa 
gacgatagg aaactggac gagtcaacc acattgacc 
tccgactat aacgaggaa ttgtacgac gatatcaat 
atggaagtg aaagtggac aagaccggg aaagcaaag 
cgcgattat tccaggacg aagaagaag gacgataaa 
gaatttgat gttctcttg gccattgaa aaggcgcaa 
agagacgaa agtgagttg gttgaagag agtttctct 
gaccgggat tacagcgaa ctggaccgt aaagacagc 
aaatcacgg attaagagc gagaatgaa agatctaac 
agtccctgg acggaggag gaagatgtg tctacaata 
cggacaagg aggcgactt tcaactcct gcgactccg 
attgattca gttccagga tcaccagtt tccagtata 
tatttttac gatgatgat cgagagtat cgcaattgg 
aagaagtct ataatgctt gtgtatagt agattggct 
gcaaataaa tacgcgtca ctgttttct aaaccaatc 
accgatgac caagctcca gggtatcac agtgtggtg 
tatagaccg atggatttg ttgacgatt agaaagaat 
atagagaat ggtgcgata agaacaacg caggagttt 
caaagagac gtccttctt atgcttaat aatgcaatt 
atgtataat aaaacaaat gatacggtt tataatatg 
gcgagacaa atgcaacaa gagtcgatg caacaaata 
cagattctg ttgcaagcg caatcgcag gatttgcct 
gttaggagg gaaactagg actagtgag ccagggtgt 
aagaggaag aggggtaat gaggagaat tccaggagt 
aagaaaagg aaggatgat tagcattac gttttgcgg 
tattcggat aagctgttt gattttagt gattttttc 
tagtattag ttttattca tattgatat aaatatgaa 
caattttat gctcgtatt gttagaatt ttcgaatac 
aaatactga tgtacagtt ctttgtagc tcgtgatca 
aatacatta atatccgtg tttaataaa tgtaagcat 
atctactta ggatttctt atgtaagca tcgttgtgt 
aaatatttt tatattatg taatataaa atacctata 
act
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY