Status
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Gene information
General information
TC031214
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010148,TC010149
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 13958348..13970771
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031214
    iBeetle screen iB_04767

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031214 RA
      MLLLLVANL IILPVAISF FNDDLSTRW IAFNCLSDT 
      IFLIDIVVN FRTGIMQQD NAEQVILDP KLIAKHYLR 
      TWFFLDLIS SIPLDYIFL IFNQDYGDS FQILHAGRA 
      LRILRLAKL LSLVRLLRL SRLVRYVSQ WEEVYFLNM 
      ASVFMRIFN LICMMLLIG HWSGCLQFL VPMLQGFPA 
      NSWVAINEL QEAFWLEQY SWALFKAMS HMLCIGYGR 
      FPPQSLTDM WLTMLSMIS GATCYALFL GHATNLIQS 
      LDSSRRQYR EKQVEEYMA YRKLPREMR QRITEYFEH 
      RYQGKFFDE ECILGELSE KLREDVINY NCRSLVASV 
      PFFANADSN FVSDVVTKL KYEVFQPGD IIIKEGTIG 
      SKMYFIQEG IVDIVMANG EVATSLSDG SYFGEICLL 
      TNARRVASV RAETYCNLF SLSVDHFNA VLDQYPLMR 
      RTMESVAAE RLNKIGKNP NLLAHREED LGSESKTIN 
      AVVNALAAE ADHVNMSDE SMARLHSSE RSLQELGRT 
      LQRLTLPRP KSENNFASQ DMPLARGEH NGRPSFHKS 
      DTFHKESAF Q
    • >TC031214 RB
      MLLLLVANL IILPVAISF FNDDLSTRW IAFNCLSDT 
      IFLIDIVVN FRTGIMQQD NAEQVILDP KLIAKHYLR 
      TWFFLDLIS SIPLDYIFL IFNQDYGDS FQILHAGRA 
      LRILRLAKL LSLVRLLRL SRLVRYVSQ WEEVYFLNM 
      ASVFMRIFN LICMMLLIG HWSGCLQFL VPMLQGFPA 
      NSWVAINEL QEAFWLEQY SWALFKAMS HMLCIGYGR 
      FPPQSLTDM WLTMLSMIS GATCYALFL GHATNLIQS 
      LDSSRRQYR EKVKQVEEY MAYRKLPRE MRQRITEYF 
      EHRYQGKFF DEECILGEL SEKLREDVI NYNCRSLVA 
      SVPFFANAD SNFVSDVVT KLKYEVFQP GDIIIKEGT 
      IGSKMYFIQ EGIVDIVMA NGEVATSLS DGSYFGEIC 
      LLTNARRVA SVRAETYCN LFSLSVDHF NAVLDQYPL 
      MRRTMESVA AERLNKIGK NPNLLAHRE EDLGSESKT 
      INAVVNALA AEADHVNMS DESMARLHS SERSLQELG 
      RTLQRLTLP RPKSENNFA SQDMPLARG EHNGRPSFH 
      KSDTFHKES AFQ
    CDS sequence
    • >TC031214 RA
      atgcttctg ttgttagtg gccaacctc ataattcta 
      ccggtggct ataagtttt ttcaacgat gacttgagc 
      acccgctgg attgccttt aactgtctg tccgataca 
      atatttcta atcgatatt gtagtcaat tttagaaca 
      gggataatg cagcaagat aacgctgaa caggtcatt 
      ctcgacccc aagttgata gcgaaacac tacctgagg 
      acgtggttt ttcctcgac ctcatctcg tccatcccc 
      ttagattat atttttctt atatttaat caagattac 
      ggcgacagt ttccagatt ctccacgcg ggccgcgcc 
      ttacgaatc ttgcggctc gcgaaattg ttatcacta 
      gtgcgactg ctgcgatta tcccgtctt gtaagatat 
      gtcagtcaa tgggaagaa gtttatttt ttaaatatg 
      gcgagtgtt ttcatgagg atattcaat ctaatatgt 
      atgatgttg ctgattggt cactggtcc ggctgtctc 
      cagttctta gtacccatg ctccaggga ttccccgcc 
      aattcttgg gtggcaatc aacgaatta caggaggca 
      ttctggctg gagcaatat tcgtgggcc ttattcaaa 
      gccatgtcg catatgctt tgcatcggt tacggccgg 
      tttccaccc cagtcgttg acagatatg tggttgacg 
      atgctatcc atgatatct ggagccaca tgttatgcc 
      ctgttcctg ggccacgcc accaacctc atccaaagt 
      ttggattcc tcaaggagg cagtatcgc gagaagcaa 
      gtggaagag tacatggca taccggaag ctgcctcgg 
      gaaatgcgt caacgcatc accgaatac ttcgagcat 
      cggtaccag ggcaagttc ttcgacgag gagtgtatc 
      ctgggcgag ctgtcggag aagctgcgc gaggacgtt 
      ataaactac aattgtcgg tcgttggtg gcgtcggtc 
      ccattcttt gctaatgcc gactcgaat ttcgtttct 
      gatgtcgta actaagttg aaatatgaa gtcttccaa 
      ccaggcgat attataata aaagaggga acgatcggt 
      tccaaaatg tacttcata caagaaggt attgtcgat 
      attgttatg gcgaacggc gaagtagcc acaagcctc 
      agtgatggc tcatatttc ggcgaaatt tgcttgttg 
      acgaatgcg agacgtgta gcctctgtg agggcagaa 
      acttattgc aacttattt tcgctatct gttgatcat 
      tttaacgct gtactcgat cagtatcca cttatgcga 
      cgcacaatg gaatctgtc gccgcggag aggttgaac 
      aagatcggc aagaacccc aacctgctg gcgcacaga 
      gaggaggac ctgggcagc gagagcaag accatcaac 
      gccgtggtg aacgcgctc gcggcggag gccgaccac 
      gtcaacatg tccgacgag tcgatggcg cgcctgcac 
      tccagcgag cgcagcctc caggagctg gggcgcacg 
      ctgcagcgg ctgacgctg ccgcggccc aagtccgag 
      aacaacttc gcctcgcag gacatgccc ctggcgcgg 
      ggcgagcac aacggccgg ccttccttc cacaaatcc 
      gacacattt cacaaggag tcggcgttc caatga
    • >TC031214 RB
      atgcttctg ttgttagtg gccaacctc ataattcta 
      ccggtggct ataagtttt ttcaacgat gacttgagc 
      acccgctgg attgccttt aactgtctg tccgataca 
      atatttcta atcgatatt gtagtcaat tttagaaca 
      gggataatg cagcaagat aacgctgaa caggtcatt 
      ctcgacccc aagttgata gcgaaacac tacctgagg 
      acgtggttt ttcctcgac ctcatctcg tccatcccc 
      ttagattat atttttctt atatttaat caagattac 
      ggcgacagt ttccagatt ctccacgcg ggccgcgcc 
      ttacgaatc ttgcggctc gcgaaattg ttatcacta 
      gtgcgactg ctgcgatta tcccgtctt gtaagatat 
      gtcagtcaa tgggaagaa gtttatttt ttaaatatg 
      gcgagtgtt ttcatgagg atattcaat ctaatatgt 
      atgatgttg ctgattggt cactggtcc ggctgtctc 
      cagttctta gtacccatg ctccaggga ttccccgcc 
      aattcttgg gtggcaatc aacgaatta caggaggca 
      ttctggctg gagcaatat tcgtgggcc ttattcaaa 
      gccatgtcg catatgctt tgcatcggt tacggccgg 
      tttccaccc cagtcgttg acagatatg tggttgacg 
      atgctatcc atgatatct ggagccaca tgttatgcc 
      ctgttcctg ggccacgcc accaacctc atccaaagt 
      ttggattcc tcaaggagg cagtatcgc gagaaggtg 
      aagcaagtg gaagagtac atggcatac cggaagctg 
      cctcgggaa atgcgtcaa cgcatcacc gaatacttc 
      gagcatcgg taccagggc aagttcttc gacgaggag 
      tgtatcctg ggcgagctg tcggagaag ctgcgcgag 
      gacgttata aactacaat tgtcggtcg ttggtggcg 
      tcggtccca ttctttgct aatgccgac tcgaatttc 
      gtttctgat gtcgtaact aagttgaaa tatgaagtc 
      ttccaacca ggcgatatt ataataaaa gagggaacg 
      atcggttcc aaaatgtac ttcatacaa gaaggtatt 
      gtcgatatt gttatggcg aacggcgaa gtagccaca 
      agcctcagt gatggctca tatttcggc gaaatttgc 
      ttgttgacg aatgcgaga cgtgtagcc tctgtgagg 
      gcagaaact tattgcaac ttattttcg ctatctgtt 
      gatcatttt aacgctgta ctcgatcag tatccactt 
      atgcgacgc acaatggaa tctgtcgcc gcggagagg 
      ttgaacaag atcggcaag aaccccaac ctgctggcg 
      cacagagag gaggacctg ggcagcgag agcaagacc 
      atcaacgcc gtggtgaac gcgctcgcg gcggaggcc 
      gaccacgtc aacatgtcc gacgagtcg atggcgcgc 
      ctgcactcc agcgagcgc agcctccag gagctgggg 
      cgcacgctg cagcggctg acgctgccg cggcccaag 
      tccgagaac aacttcgcc tcgcaggac atgcccctg 
      gcgcggggc gagcacaac ggccggcct tccttccac 
      aaatccgac acatttcac aaggagtcg gcgttccaa 
      tga
    mRNA sequence
    • >TC031214 RA
      atttttatg cctaacaat acaaattaa ccaattaac 
      atctgttac tagcgattt ggttattta attttactg 
      ggatttgtg tatgcttct gttgttagt ggccaacct 
      cataattct accggtggc tataagttt tttcaacga 
      tgacttgag cacccgctg gattgcctt taactgtct 
      gtccgatac aatatttct aatcgatat tgtagtcaa 
      ttttagaac agggataat gcagcaaga taacgctga 
      acaggtcat tctcgaccc caagttgat agcgaaaca 
      ctacctgag gacgtggtt tttcctcga cctcatctc 
      gtccatccc cttagatta tatttttct tatatttaa 
      tcaagatta cggcgacag tttccagat tctccacgc 
      gggccgcgc cttacgaat cttgcggct cgcgaaatt 
      gttatcact agtgcgact gctgcgatt atcccgtct 
      tgtaagata tgtcagtca atgggaaga agtttattt 
      tttaaatat ggcgagtgt tttcatgag gatattcaa 
      tctaatatg tatgatgtt gctgattgg tcactggtc 
      cggctgtct ccagttctt agtacccat gctccaggg 
      attccccgc caattcttg ggtggcaat caacgaatt 
      acaggaggc attctggct ggagcaata ttcgtgggc 
      cttattcaa agccatgtc gcatatgct ttgcatcgg 
      ttacggccg gtttccacc ccagtcgtt gacagatat 
      gtggttgac gatgctatc catgatatc tggagccac 
      atgttatgc cctgttcct gggccacgc caccaacct 
      catccaaag tttggattc ctcaaggag gcagtatcg 
      cgagaagca agtggaaga gtacatggc ataccggaa 
      gctgcctcg ggaaatgcg tcaacgcat caccgaata 
      cttcgagca tcggtacca gggcaagtt cttcgacga 
      ggagtgtat cctgggcga gctgtcgga gaagctgcg 
      cgaggacgt tataaacta caattgtcg gtcgttggt 
      ggcgtcggt cccattctt tgctaatgc cgactcgaa 
      tttcgtttc tgatgtcgt aactaagtt gaaatatga 
      agtcttcca accaggcga tattataat aaaagaggg 
      aacgatcgg ttccaaaat gtacttcat acaagaagg 
      tattgtcga tattgttat ggcgaacgg cgaagtagc 
      cacaagcct cagtgatgg ctcatattt cggcgaaat 
      ttgcttgtt gacgaatgc gagacgtgt agcctctgt 
      gagggcaga aacttattg caacttatt ttcgctatc 
      tgttgatca ttttaacgc tgtactcga tcagtatcc 
      acttatgcg acgcacaat ggaatctgt cgccgcgga 
      gaggttgaa caagatcgg caagaaccc caacctgct 
      ggcgcacag agaggagga cctgggcag cgagagcaa 
      gaccatcaa cgccgtggt gaacgcgct cgcggcgga 
      ggccgacca cgtcaacat gtccgacga gtcgatggc 
      gcgcctgca ctccagcga gcgcagcct ccaggagct 
      ggggcgcac gctgcagcg gctgacgct gccgcggcc 
      caagtccga gaacaactt cgcctcgca ggacatgcc 
      cctggcgcg gggcgagca caacggccg gccttcctt 
      ccacaaatc cgacacatt tcacaagga gtcggcgtt 
      ccaatgacg ccactccac gcactagtg ctcggggtt 
      accaggaca gctgtgtca actgccggt gctggcgct 
      cgccacggc ggcactggc aattcgcac agaccgccg 
      ctgaactga cgacaaact tggggtgct ggtgcgtgc 
      cgctttgct gtgtcttgt attaagcaa tacgtgcga 
      cggacgtta tgctagaat tgtgcaata ttagatact 
      acattaagt tagctttaa ttattaggt gctcaataa 
      atttaatta tcctagaat aacaccgcc cggtttttg 
      gcagaacgc accagcagc tggacaatt tattgcaaa 
      cacgcgcaa actaccgcg atcatatca acttttgta 
      attacaatc agtgtcaga ttgttgaca aaggacctc 
      gaattaatg acaatgttt taatttaac aaaatacaa 
      taaaaatat aaatgaaaa aacagtagt gtcctggaa 
      tactggaag atgattttg cattaaact aaccaatat 
      cgttttatc tgttttgat tcaaaagaa ccgttgtgg 
      ttggttttg gtttttgta gatttcttc tgaaataaa 
      ccttcagca aaaggtgat tagtgactt ttacaaaaa 
      aagataggg taataacgt aatgaaaga cttaataat 
      tttcaaatg caagagcat cattaaaaa actttaaaa 
      tctgtgacc ataataaaa ctttataaa aacattgtg 
      atacttaaa acaaacaaa taaaagcaa cggaataac 
      gaaaaataa aaattaagt aaatgctat taataatat 
      aaattttat tgactttct atttagttt tcaatattt 
      tgcaattat ttcccattg tcctagttt tctgtatct 
      tccatatct gcacaaaaa tcgcttgaa cagaacaga 
      atttaaatt ttgcatttt atcttattt ttgtcaaat 
      gtgacatcg aggtattgt ttgtacatg ttttacata 
      aaataaaat aaaccgcgt tacgatatt cctaacgtt 
      atttgtttc aatttggct attttatgt gaggttttt 
      tgctatgtg tgtaaagtt acactttac taaacgatt 
      ttaaactta gtaaagttt tcgattact gaattaaat 
      acgaattgt ttttagtat atttcctac tatcaagta 
      atatgttgg aaatgctta aggaggaaa ctgttaaac 
      caaatgttc gaatgaaaa aaatggaaa aaattaaat 
      tgtaataaa gaaatcatc ttgtt
    • >TC031214 RB
      atttttatg cctaacaat acaaattaa ccaattaac 
      atctgttac tagcgattt ggttattta attttactg 
      ggatttgtg tatgcttct gttgttagt ggccaacct 
      cataattct accggtggc tataagttt tttcaacga 
      tgacttgag cacccgctg gattgcctt taactgtct 
      gtccgatac aatatttct aatcgatat tgtagtcaa 
      ttttagaac agggataat gcagcaaga taacgctga 
      acaggtcat tctcgaccc caagttgat agcgaaaca 
      ctacctgag gacgtggtt tttcctcga cctcatctc 
      gtccatccc cttagatta tatttttct tatatttaa 
      tcaagatta cggcgacag tttccagat tctccacgc 
      gggccgcgc cttacgaat cttgcggct cgcgaaatt 
      gttatcact agtgcgact gctgcgatt atcccgtct 
      tgtaagata tgtcagtca atgggaaga agtttattt 
      tttaaatat ggcgagtgt tttcatgag gatattcaa 
      tctaatatg tatgatgtt gctgattgg tcactggtc 
      cggctgtct ccagttctt agtacccat gctccaggg 
      attccccgc caattcttg ggtggcaat caacgaatt 
      acaggaggc attctggct ggagcaata ttcgtgggc 
      cttattcaa agccatgtc gcatatgct ttgcatcgg 
      ttacggccg gtttccacc ccagtcgtt gacagatat 
      gtggttgac gatgctatc catgatatc tggagccac 
      atgttatgc cctgttcct gggccacgc caccaacct 
      catccaaag tttggattc ctcaaggag gcagtatcg 
      cgagaaggt gaagcaagt ggaagagta catggcata 
      ccggaagct gcctcggga aatgcgtca acgcatcac 
      cgaatactt cgagcatcg gtaccaggg caagttctt 
      cgacgagga gtgtatcct gggcgagct gtcggagaa 
      gctgcgcga ggacgttat aaactacaa ttgtcggtc 
      gttggtggc gtcggtccc attctttgc taatgccga 
      ctcgaattt cgtttctga tgtcgtaac taagttgaa 
      atatgaagt cttccaacc aggcgatat tataataaa 
      agagggaac gatcggttc caaaatgta cttcataca 
      agaaggtat tgtcgatat tgttatggc gaacggcga 
      agtagccac aagcctcag tgatggctc atatttcgg 
      cgaaatttg cttgttgac gaatgcgag acgtgtagc 
      ctctgtgag ggcagaaac ttattgcaa cttattttc 
      gctatctgt tgatcattt taacgctgt actcgatca 
      gtatccact tatgcgacg cacaatgga atctgtcgc 
      cgcggagag gttgaacaa gatcggcaa gaaccccaa 
      cctgctggc gcacagaga ggaggacct gggcagcga 
      gagcaagac catcaacgc cgtggtgaa cgcgctcgc 
      ggcggaggc cgaccacgt caacatgtc cgacgagtc 
      gatggcgcg cctgcactc cagcgagcg cagcctcca 
      ggagctggg gcgcacgct gcagcggct gacgctgcc 
      gcggcccaa gtccgagaa caacttcgc ctcgcagga 
      catgcccct ggcgcgggg cgagcacaa cggccggcc 
      ttccttcca caaatccga cacatttca caaggagtc 
      ggcgttcca atgacgcca ctccacgca ctagtgctc 
      ggggttacc aggacagct gtgtcaact gccggtgct 
      ggcgctcgc cacggcggc actggcaat tcgcacaga 
      ccgccgctg aactgacga caaacttgg ggtgctggt 
      gcgtgccgc tttgctgtg tcttgtatt aagcaatac 
      gtgcgacgg acgttatgc tagaattgt gcaatatta 
      gatactaca ttaagttag ctttaatta ttaggtgct 
      caataaatt taattatcc tagaataac accgcccgg 
      tttttggca gaacgcacc agcagctgg acaatttat 
      tgcaaacac gcgcaaact accgcgatc atatcaact 
      tttgtaatt acaatcagt gtcagattg ttgacaaag 
      gacctcgaa ttaatgaca atgttttaa tttaacaaa 
      atacaataa aaatataaa tgaaaaaac agtagtgtc 
      ctggaatac tggaagatg attttgcat taaactaac 
      caatatcgt tttatctgt tttgattca aaagaaccg 
      ttgtggttg gttttggtt tttgtagat ttcttctga 
      aataaacct tcagcaaaa ggtgattag tgactttta 
      caaaaaaag atagggtaa taacgtaat gaaagactt 
      aataatttt caaatgcaa gagcatcat taaaaaact 
      ttaaaatct gtgaccata ataaaactt tataaaaac 
      attgtgata cttaaaaca aacaaataa aagcaacgg 
      aataacgaa aaataaaaa ttaagtaaa tgctattaa 
      taatataaa ttttattga ctttctatt tagttttca 
      atattttgc aattatttc ccattgtcc tagttttct 
      gtatcttcc atatctgca caaaaatcg cttgaacag 
      aacagaatt taaattttg cattttatc ttatttttg 
      tcaaatgtg acatcgagg tattgtttg tacatgttt 
      tacataaaa taaaataaa ccgcgttac gatattcct 
      aacgttatt tgtttcaat ttggctatt ttatgtgag 
      gttttttgc tatgtgtgt aaagttaca ctttactaa 
      acgatttta aacttagta aagttttcg attactgaa 
      ttaaatacg aattgtttt tagtatatt tcctactat 
      caagtaata tgttggaaa tgcttaagg aggaaactg 
      ttaaaccaa atgttcgaa tgaaaaaaa tggaaaaaa 
      ttaaattgt aataaagaa atcatcttg tt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY