Status
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Gene information
General information
TC010130 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 14250965..14282996
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010130
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04765

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010130 RA
      MAIYSQNFV KYIVVLLYL VQFINGSSH FLRNVDNAM 
      YRHRRSYEL RRKGKESVV DSNSVKSVS NGNKSGVFA 
      TENCTVVVA QIGGTATLP CVVRKFNNG VVSWIRKRD 
      YHLLTVGPT TYNTDDRFL VEHVRHLQN WGLLIKHVQ 
      LSDAGFYEC QMSTHPPTS ILIELKVTK ALAEIQGAP 
      DLYMRAGSL LRLVCTLRH STEPPAYVF WYHEQKMIN 
      YDPGVTVKE GRSSSVLLL QDADKSHNG NYTCSPSNA 
      VPASINVHV LNATAEEKP AAMQHANTS SSSSYSFAR 
      CSILLIALI PVIPNT
    CDS sequence
    • >TC010130 RA
      atggctata tattctcaa aatttcgta aaatacatc 
      gtggttttg ttgtattta gtccagttc atcaacgga 
      tcctcgcac tttttgcga aacgtcgac aatgccatg 
      tatcggcac agaaggagt tacgagctt cgtcggaaa 
      ggaaaagaa agtgttgta gatagcaat tcggtgaaa 
      agtgtgtca aatggcaat aagtctgga gtttttgca 
      acggaaaat tgcactgtg gtcgtcgca caaattgga 
      gggacggcg acgttgcct tgcgtcgtt agaaaattc 
      aataatggt gtcgtctcg tggattcgc aaacgggat 
      tatcacctc ctgacagtc ggtccaacg acatacaat 
      accgacgac cgcttcttg gtggaacat gttcgacat 
      ctgcaaaac tggggtctg cttatcaag cacgtgcag 
      ctctcggat gccggcttc tacgagtgc caaatgtcg 
      actcatcca cccacaagt attctcatc gaactcaag 
      gtcactaag gccttagcg gaaatccag ggtgccccc 
      gacttgtac atgcgagcg gggtcgttg cttcgctta 
      gtttgtacc ttgcggcac tcgacggag ccgcccgcc 
      tacgttttc tggtatcac gagcagaaa atgattaac 
      tacgacccg ggggtgacc gtcaaggag ggccgctcc 
      tccagcgtg ctcctgctc caggacgcc gataagagc 
      cacaacggc aactacact tgcagcccc tccaacgcg 
      gtgcccgca tccattaac gtccacgtc cttaacgcc 
      actgcagag gagaaacca gctgccatg cagcacgcc 
      aacaccagc tcatcctcg tcgtattcc ttcgccagg 
      tgcagcatt cttctcatt gccctcatt cccgtgata 
      ccaaacacg tga
    mRNA sequence
    • >TC010130 RA
      aagccaact gtgaactac tcggaaaag aataacaag 
      agagggagc ttggaaggg aaaaagttt tgcggcgag 
      ggcgcaaaa agcagggtg aattcagtg agtggggga 
      cattaagct aaaccaaaa tttagtgaa tgttaaact 
      agagtttgc caatgacgt gagttgcaa gaccgctaa 
      aatcaagat ggatgtcgt ctgaaaaaa gtgctggga 
      ctggaaacg agaatggct atatattct caaaatttc 
      gtaaaatac atcgtggtt ttgttgtat ttagtccag 
      ttcatcaac ggatcctcg cactttttg cgaaacgtc 
      gacaatgcc atgtatcgg cacagaagg agttacgag 
      cttcgtcgg aaaggaaaa gaaagtgtt gtagatagc 
      aattcggtg aaaagtgtg tcaaatggc aataagtct 
      ggagttttt gcaacggaa aattgcact gtggtcgtc 
      gcacaaatt ggagggacg gcgacgttg ccttgcgtc 
      gttagaaaa ttcaataat ggtgtcgtc tcgtggatt 
      cgcaaacgg gattatcac ctcctgaca gtcggtcca 
      acgacatac aataccgac gaccgcttc ttggtggaa 
      catgttcga catctgcaa aactggggt ctgcttatc 
      aagcacgtg cagctctcg gatgccggc ttctacgag 
      tgccaaatg tcgactcat ccacccaca agtattctc 
      atcgaactc aaggtcact aaggcctta gcggaaatc 
      cagggtgcc cccgacttg tacatgcga gcggggtcg 
      ttgcttcgc ttagtttgt accttgcgg cactcgacg 
      gagccgccc gcctacgtt ttctggtat cacgagcag 
      aaaatgatt aactacgac ccgggggtg accgtcaag 
      gagggccgc tcctccagc gtgctcctg ctccaggac 
      gccgataag agccacaac ggcaactac acttgcagc 
      ccctccaac gcggtgccc gcatccatt aacgtccac 
      gtccttaac gccactgca gaggagaaa ccagctgcc 
      atgcagcac gccaacacc agctcatcc tcgtcgtat 
      tccttcgcc aggtgcagc attcttctc attgccctc 
      attcccgtg ataccaaac acgtgatcc tgacaaggg 
      acgggatat atggactat ttgtgatgt tttacaagt 
      gatatcatg gtacttcgt aagcctaac tcaactttg 
      taaatttgt aaatatttg tttataaac gttcaacta 
      aaattggaa tatttatca ttgtatcat tgccaaaca 
      cttccatgt aatattact atgtcaaat aattcgatt 
      ttattttta ttgaataaa cgattcaat attaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY