Status
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Gene information
General information
TC010113 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 14579164..14645100
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010113
    iBeetle screen iB_04764

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    leg musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    labrum not present - Penetrance (percentage of animals/eggs on slide): more than 80%

    antenna sometimes shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    labium swollen - Penetrance (percentage of animals/eggs on slide): more than 80%

    maxillary palp surface irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    Comment: stripes are missing
    labium distal surface irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    Comment: stripes are missing
    legs trochanter size decreased - Penetrance (percentage of animals/eggs on slide): more than 80%

    legs tibiotarsus size decreased - Penetrance (percentage of animals/eggs on slide): more than 80%

    legs tibiotarsus potentially fused - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs shortened - Penetrance (percentage of animals/eggs on slide): more than 80%

    abdomen tracheal opening not present - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection



    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010113 RA
      MRQCIHYRK LTACTSKSG PSAKNTRSS RLHPRIRIE 
      LKKPVYVSS ITYGEDLQE TVALRHLAT TKKPINSSP 
      EETSFEPKK TRAFYFIFH QTDVNVYHD YPSRGVLTV 
      LTLQIVNRI ATNDIIEEA TYSGIIDPS PEWHTLNHR 
      GPKATLTYR VRVKCDSHY YNATCTKFC RPRDDKFGH 
      YICDANGDK ECIEGWKGA TCDFAVCKP GCHPVHGKC 
      DNPGECNCR PGWRGDFCE QCEPYPGCK HGYCNGSSW 
      QCICDTNWG GILCDQDLN YCGTHEPCL NGGTCENTA 
      PDTYLCTCP EGFSGTNCE VVDNPCAPQ PCLHGGTCL 
      EAGGSFSCE CAPGWTGPT CNVDIDECA SAPCQNGGT 
      CIDLEDMFK CECPPAWEG NVCQFDADE CLTAPCINA 
      ISCANLVGD YRCKCRVGW MGKNCDSNI NDCVGQCQH 
      GATCIDLVN DYHCACQPG YTGRDCHTD IDDCASNPC 
      KNGGECVDQ VNGYRCICP VGFTGHECE NDYNHCSPD 
      PCQNGASCF NTQTDYYCH CPEGWLGKN CSESKAICD 
      NPPCDDEVE SCIAVGVGS GRNSAPSIC GEHGHCVNL 
      PGVGHRCQC QPGFTGKYC HENINDCKV NPCENGGTC 
      VDKINAFQC ICKEGWEGA LCNINTNDC SPNPCRNNG 
      SCVDRIADF ECNCKNGWK GKTCSLKDS HCDHTTCKN 
      GGTCQDLGK TFMCRCPPD WEGTTCHIA KQTACRSNP 
      CLNGGTCVN TGDYYQCIC RDGFEGVHC EDDVNDCIP 
      QPCQNGGKC IDGVNWFLC ECAPGFTGP DCKINVNDC 
      ASNPCGYGG TCLDRIDDF DCICSPGRS GKQCNGSCT 
      WEGHTLENN ITWQHECNT CLCLNGLVK CTRIWCGLG 
      NCRSSERVN CNNNQVCVP SPSESCLSP PCQPYGECR 
      DLESGRRVK PPSVPSPST CWPNQAVLS NTCARLTLL 
      LDRPKLPPG VTVESLCGD LRRLLANQQ AANDLQNEL 
      VLLCDLKMD YNDTVEVTL SGEEGVLDG IRVIGEAIS 
      RKQTTLLAL TSIVEVKVE TALVSEEQQ NNKYLIALV 
      CFIVIGLMG ALTVALLYV RQRRRNLGL SGMNLSPSS 
      DTCHRNHED EKSNNLQNE ENLRRYANP LKDDGGSMA 
      SINSAGACG LDLPKVSVV RPLSSMLPH DGSSEMLEM 
      ISEVDCPGS RKTMLVVPG TSNEANSMK LGDGLSPAH 
      RSSQIMLYK AQNPDVRKN TAAFDDSSG HKDFSKSII 
      NVNKQRTSQ NTSGSNDVL TVLV
    CDS sequence
    • >TC010113 RA
      atgcggcag tgcatacat tacaggaaa ctcacagcg 
      tgtacttcc aagagtggg ccctcggca aaaaacaca 
      cgatcatca cgattgcat ccgagaatc cggattgaa 
      ttgaagaaa cccgtctat gtgtcatca ataacgtat 
      ggtgaagac ctgcaggag acggtggca cttcgccac 
      cttgccacg accaaaaag cccatcaac agctccccg 
      gaggaaact tcattcgaa ccaaaaaag acccgagct 
      ttctacttt atttttcat caaaccgac gtaaatgtt 
      tatcacgac tatccatct cgtggcgtt ttgacggtt 
      ttgacgtta cagattgta aatagaata gctactaac 
      gatatcatt gaggaagca acgtattcg gggatcatc 
      gatccgagc cccgagtgg cacaccctg aaccacagg 
      ggtccaaaa gccacgctc acctacagg gtgcgtgtc 
      aaatgtgat agtcactat tacaatgcc acctgtacc 
      aagttctgt cgaccgcgt gatgacaag tttgggcat 
      tatatctgc gacgccaac ggcgacaaa gagtgcata 
      gagggctgg aagggcgcc acttgtgat ttcgctgtg 
      tgtaaaccc ggctgccat cccgtccac ggcaaatgt 
      gataatccc ggcgaatgc aattgccgg cctgggtgg 
      cgaggcgat ttctgcgag caatgcgaa ccctatcct 
      gggtgcaag catggctac tgcaatggg tcctcctgg 
      cagtgcatt tgcgacacc aactggggc ggcattctc 
      tgcgaccaa gatttaaat tactgcgga acccatgaa 
      ccgtgtttg aatgggggc acctgtgag aacacggcc 
      ccggacact tacttgtgc acatgtccg gaaggattt 
      tccggcacg aactgcgaa gttgtggac aatccatgc 
      gccccccag ccctgcctc cacggaggg acgtgtctg 
      gaagccgga ggctccttt agttgtgaa tgcgccccc 
      ggatggacg gggcccacc tgcaatgtg gacattgat 
      gaatgcgct tctgctccg tgtcagaac ggaggaacg 
      tgcatcgat ctggaggac atgttcaag tgcgaatgt 
      cccccagct tgggaaggc aacgtttgc caattcgat 
      gcagacgag tgcctgacg gccccttgc atcaatgcc 
      atctcctgt gcgaatctg gtcggagat tatcggtgc 
      aaatgtcga gtcggttgg atgggaaaa aactgcgat 
      agtaacatc aacgattgt gtgggacaa tgccaacac 
      ggcgccact tgtatcgat ttggtcaac gattaccac 
      tgtgcctgt caaccaggc tacacaggt cgcgattgc 
      cacacggac atcgacgac tgcgcctca aacccttgc 
      aaaaacggt ggcgagtgc gtggaccaa gtgaacggt 
      tatcgctgc atctgtcct gtgggattc accggccac 
      gagtgcgag aacgactac aaccattgc agtcccgac 
      ccgtgccaa aacggcgct tcctgcttc aacacgcaa 
      acggactac tactgccat tgtcctgaa ggatggctg 
      gggaagaac tgcagcgag agcaaggcg atttgcgac 
      aatccgccc tgcgatgac gaagtggaa agttgcata 
      gctgtggga gtaggaagt ggaaggaac agcgccccc 
      agcatttgt ggcgaacac gggcactgc gtgaaccta 
      cccggagtc ggacatcgc tgccaatgt cagcccgga 
      ttcaccgga aagtattgc cacgagaat ataaacgac 
      tgtaaagta aatccctgc gaaaatggc gggacttgc 
      gtcgacaaa atcaacgcc tttcagtgt atttgcaaa 
      gagggttgg gaaggcgct ctgtgcaac ataaacacg 
      aacgactgc agcccgaac ccttgccga aacaacggc 
      agttgcgtg gatcgaatc gccgacttc gagtgcaac 
      tgcaagaac ggctggaag ggcaaaacg tgctctctc 
      aaagacagc cactgcgac cacaccacg tgcaagaac 
      ggcggcact tgccaggat ttgggcaag acgttcatg 
      tgccgatgc cctcccgac tgggaaggc accacttgt 
      cacatcgcc aaacagacg gcgtgccgc tccaacccc 
      tgcctcaac ggcggcacc tgcgtcaac accggcgac 
      tactaccag tgcatctgc agggacggc ttcgagggc 
      gtgcactgc gaggacgac gtcaacgac tgcatcccc 
      cagccgtgc cagaacggc ggcaagtgc atcgacggc 
      gtcaactgg ttcctctgc gagtgcgcc cctggattc 
      acaggaccg gactgcaag atcaacgtc aacgactgc 
      gcctccaac ccctgcgga tacggaggc acttgcttg 
      gatagaatc gacgatttt gattgcatt tgctcgccc 
      ggaaggagc ggaaagcag tgtaacgga agttgcaca 
      tgggaaggc cacaccttg gagaacaac atcacgtgg 
      cagcacgaa tgcaatacg tgtctgtgc ctcaacgga 
      ttggtgaaa tgtacgagg atttggtgc ggtctggga 
      aactgcagg agttccgag agggtcaac tgcaataat 
      aaccaggtt tgcgtcccg tcgccgtcc gagtcgtgc 
      ttgtcaccg ccgtgtcaa ccctatgga gagtgcagg 
      gacttggaa agtggtcga agggtgaaa ccgccgtcg 
      gtgcccagt cctagcact tgttggccc aatcaggcc 
      gtcctcagc aacacttgt gccaggtta actttactc 
      ttggacagg cccaaatta ccaccaggt gtgaccgtg 
      gagagtctt tgtggcgat ttgaggcgg cttttggcc 
      aaccagcag gcagctaat gacttgcaa aatgaattg 
      gttctactg tgtgatctt aagatggac tacaacgac 
      accgtcgaa gtgactctg tctggcgag gaaggcgtc 
      ttggacgga atccgcgtg atcggcgag gcaatatcc 
      cgcaaacag acaaccctt ttagccctc acctccatc 
      gtcgaagta aaagttgag acggccctc gtgtccgaa 
      gagcaacaa aacaacaaa tatctgatc gcactcgtg 
      tgcttcatc gtcatcggc ttgatgggc gccctcacc 
      gtggccctc ctctacgtc cggcagcgg cggcgcaac 
      ctcggcctc agcggcatg aacctctcc ccctcctcc 
      gacacctgc caccgcaac cacgaggac gagaagtcc 
      aacaacctc caaaacgaa gagaacctg cgcagatac 
      gccaacccc ttgaaggac gacggcggc agcatggcc 
      agcatcaac tcggcgggc gcctgcggc ctggacctg 
      cccaaggtg agcgtggtg cggcctttg agctcgatg 
      ctgccgcac gacggctcc agcgagatg ctggagatg 
      atctccgag gtggactgc cccgggtcc aggaagacc 
      atgctggtg gtgcccggg accagcaac gaggccaac 
      agcatgaag ctgggcgat gggctgagt ccggcccat 
      cgcagcagc cagatcatg ctctacaaa gcgcaaaac 
      cccgatgtg aggaaaaac acggcggct ttcgacgac 
      tcctcggga cacaaagac tttagcaaa agtattatt 
      aatgtgaat aaacagagg acttcgcag aacacgagc 
      gggagtaac gacgtgctc acggtgctt gtgtaa
    mRNA sequence
    • >TC010113 RA
      atgcggcag tgcatacat tacaggaaa ctcacagcg 
      tgtacttcc aagagtggg ccctcggca aaaaacaca 
      cgatcatca cgattgcat ccgagaatc cggattgaa 
      ttgaagaaa cccgtctat gtgtcatca ataacgtat 
      ggtgaagac ctgcaggag acggtggca cttcgccac 
      cttgccacg accaaaaag cccatcaac agctccccg 
      gaggaaact tcattcgaa ccaaaaaag acccgagct 
      ttctacttt atttttcat caaaccgac gtaaatgtt 
      tatcacgac tatccatct cgtggcgtt ttgacggtt 
      ttgacgtta cagattgta aatagaata gctactaac 
      gatatcatt gaggaagca acgtattcg gggatcatc 
      gatccgagc cccgagtgg cacaccctg aaccacagg 
      ggtccaaaa gccacgctc acctacagg gtgcgtgtc 
      aaatgtgat agtcactat tacaatgcc acctgtacc 
      aagttctgt cgaccgcgt gatgacaag tttgggcat 
      tatatctgc gacgccaac ggcgacaaa gagtgcata 
      gagggctgg aagggcgcc acttgtgat ttcgctgtg 
      tgtaaaccc ggctgccat cccgtccac ggcaaatgt 
      gataatccc ggcgaatgc aattgccgg cctgggtgg 
      cgaggcgat ttctgcgag caatgcgaa ccctatcct 
      gggtgcaag catggctac tgcaatggg tcctcctgg 
      cagtgcatt tgcgacacc aactggggc ggcattctc 
      tgcgaccaa gatttaaat tactgcgga acccatgaa 
      ccgtgtttg aatgggggc acctgtgag aacacggcc 
      ccggacact tacttgtgc acatgtccg gaaggattt 
      tccggcacg aactgcgaa gttgtggac aatccatgc 
      gccccccag ccctgcctc cacggaggg acgtgtctg 
      gaagccgga ggctccttt agttgtgaa tgcgccccc 
      ggatggacg gggcccacc tgcaatgtg gacattgat 
      gaatgcgct tctgctccg tgtcagaac ggaggaacg 
      tgcatcgat ctggaggac atgttcaag tgcgaatgt 
      cccccagct tgggaaggc aacgtttgc caattcgat 
      gcagacgag tgcctgacg gccccttgc atcaatgcc 
      atctcctgt gcgaatctg gtcggagat tatcggtgc 
      aaatgtcga gtcggttgg atgggaaaa aactgcgat 
      agtaacatc aacgattgt gtgggacaa tgccaacac 
      ggcgccact tgtatcgat ttggtcaac gattaccac 
      tgtgcctgt caaccaggc tacacaggt cgcgattgc 
      cacacggac atcgacgac tgcgcctca aacccttgc 
      aaaaacggt ggcgagtgc gtggaccaa gtgaacggt 
      tatcgctgc atctgtcct gtgggattc accggccac 
      gagtgcgag aacgactac aaccattgc agtcccgac 
      ccgtgccaa aacggcgct tcctgcttc aacacgcaa 
      acggactac tactgccat tgtcctgaa ggatggctg 
      gggaagaac tgcagcgag agcaaggcg atttgcgac 
      aatccgccc tgcgatgac gaagtggaa agttgcata 
      gctgtggga gtaggaagt ggaaggaac agcgccccc 
      agcatttgt ggcgaacac gggcactgc gtgaaccta 
      cccggagtc ggacatcgc tgccaatgt cagcccgga 
      ttcaccgga aagtattgc cacgagaat ataaacgac 
      tgtaaagta aatccctgc gaaaatggc gggacttgc 
      gtcgacaaa atcaacgcc tttcagtgt atttgcaaa 
      gagggttgg gaaggcgct ctgtgcaac ataaacacg 
      aacgactgc agcccgaac ccttgccga aacaacggc 
      agttgcgtg gatcgaatc gccgacttc gagtgcaac 
      tgcaagaac ggctggaag ggcaaaacg tgctctctc 
      aaagacagc cactgcgac cacaccacg tgcaagaac 
      ggcggcact tgccaggat ttgggcaag acgttcatg 
      tgccgatgc cctcccgac tgggaaggc accacttgt 
      cacatcgcc aaacagacg gcgtgccgc tccaacccc 
      tgcctcaac ggcggcacc tgcgtcaac accggcgac 
      tactaccag tgcatctgc agggacggc ttcgagggc 
      gtgcactgc gaggacgac gtcaacgac tgcatcccc 
      cagccgtgc cagaacggc ggcaagtgc atcgacggc 
      gtcaactgg ttcctctgc gagtgcgcc cctggattc 
      acaggaccg gactgcaag atcaacgtc aacgactgc 
      gcctccaac ccctgcgga tacggaggc acttgcttg 
      gatagaatc gacgatttt gattgcatt tgctcgccc 
      ggaaggagc ggaaagcag tgtaacgga agttgcaca 
      tgggaaggc cacaccttg gagaacaac atcacgtgg 
      cagcacgaa tgcaatacg tgtctgtgc ctcaacgga 
      ttggtgaaa tgtacgagg atttggtgc ggtctggga 
      aactgcagg agttccgag agggtcaac tgcaataat 
      aaccaggtt tgcgtcccg tcgccgtcc gagtcgtgc 
      ttgtcaccg ccgtgtcaa ccctatgga gagtgcagg 
      gacttggaa agtggtcga agggtgaaa ccgccgtcg 
      gtgcccagt cctagcact tgttggccc aatcaggcc 
      gtcctcagc aacacttgt gccaggtta actttactc 
      ttggacagg cccaaatta ccaccaggt gtgaccgtg 
      gagagtctt tgtggcgat ttgaggcgg cttttggcc 
      aaccagcag gcagctaat gacttgcaa aatgaattg 
      gttctactg tgtgatctt aagatggac tacaacgac 
      accgtcgaa gtgactctg tctggcgag gaaggcgtc 
      ttggacgga atccgcgtg atcggcgag gcaatatcc 
      cgcaaacag acaaccctt ttagccctc acctccatc 
      gtcgaagta aaagttgag acggccctc gtgtccgaa 
      gagcaacaa aacaacaaa tatctgatc gcactcgtg 
      tgcttcatc gtcatcggc ttgatgggc gccctcacc 
      gtggccctc ctctacgtc cggcagcgg cggcgcaac 
      ctcggcctc agcggcatg aacctctcc ccctcctcc 
      gacacctgc caccgcaac cacgaggac gagaagtcc 
      aacaacctc caaaacgaa gagaacctg cgcagatac 
      gccaacccc ttgaaggac gacggcggc agcatggcc 
      agcatcaac tcggcgggc gcctgcggc ctggacctg 
      cccaaggtg agcgtggtg cggcctttg agctcgatg 
      ctgccgcac gacggctcc agcgagatg ctggagatg 
      atctccgag gtggactgc cccgggtcc aggaagacc 
      atgctggtg gtgcccggg accagcaac gaggccaac 
      agcatgaag ctgggcgat gggctgagt ccggcccat 
      cgcagcagc cagatcatg ctctacaaa gcgcaaaac 
      cccgatgtg aggaaaaac acggcggct ttcgacgac 
      tcctcggga cacaaagac tttagcaaa agtattatt 
      aatgtgaat aaacagagg acttcgcag aacacgagc 
      gggagtaac gacgtgctc acggtgctt gtgtaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY