Status
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Gene information
General information
TC010108 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 14842664..14844842
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010108
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04763

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 6 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 6 (19 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 4 (22 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010108 RA
      MAHGKTEKC EQDCYKGGT VLFMYLTGL GIRYWLMFS 
      RYQSIIANH IEISTPLNS WKRVSEGLC LQSKGINPY 
      EGDLLHEAP ITILFYKVL TQWWKLDIK TIFLAFDAG 
      TAVVLYFVA KKYMFHLFI EQEENKKQY ALNSKEFLL 
      SGSDFVNSP VFVASAFLF NPYTIFNCV GQSTVVFHN 
      FFIALFLFC MLSGSVILS VLSLAICTM MSFYPIVLI 
      IPLFLYSNG IYKSKFRAI LTPILFIAT LAAFITISI 
      GLSNGTAFM KNVYGFILT VPDLQPNIG LFWYFFTEM 
      FDHFRDLFI SSFQINATV LYLIPLSVK FRKDPFLLT 
      FAILCLISV FKSYPCLGD VGFVLSLLP CFIHLFNYS 
      QQGFLVGVI FLITTALGP ILWHLWIYS NSANANFYF 
      GVTLAFAIA QIFLVTDIL FAYLKREFC LNHGKERKI 
      DGKDALLIL E
    CDS sequence
    • >TC010108 RA
      atggcacat gggaaaacc gaaaaatgt gagcaagac 
      tgctacaaa ggcggaacc gttttattc atgtacttg 
      acggggctt ggtatccgg tattggctc atgttttcg 
      agatatcag tccataatt gcgaaccac atcgaaata 
      tctacacct ctgaactcc tggaagcga gtgtccgaa 
      gggctttgt ctccagtcc aaaggtatc aatccatac 
      gaaggggac ttacttcat gaagctccg ataacaata 
      ttattttat aaagtgttg acacagtgg tggaagttg 
      gacattaag actattttt ctcgccttt gatgcggga 
      acggccgtg gtattgtat ttcgtcgcg aagaaatac 
      atgtttcac ttatttatt gaacaagaa gagaataaa 
      aaacaatat gctctgaac agtaaggag ttcctgtta 
      tcgggctct gatttcgtc aattcgccc gtttttgtc 
      gcatcggcg tttctgttt aatccatac acgattttt 
      aactgcgtt ggacagtca actgtcgtg ttccataat 
      tttttcatc gctttgttt ttgttctgt atgttaagc 
      ggtagcgtt attttgagt gtactgtct ctggcaata 
      tgcacaatg atgtctttc taccccatt gtgttaatt 
      atcccactg tttttgtac tctaacgga atttacaaa 
      agcaaattt cgggcgatt ctcaccccg attttgttt 
      atcgccaca cttgcggct tttataacc atttcgatt 
      ggtttaagc aacggaacc gcatttatg aaaaacgtg 
      tacggtttc attctaaca gttccagac ttgcagcca 
      aatattggc ttgttttgg tattttttc accgaaatg 
      ttcgaccac tttcgcgac ttgttcatc agctcgttt 
      caaataaac gcaaccgtt ttgtattta attccactg 
      tcggtcaaa tttcgcaaa gacccgttt ttgctaacg 
      ttcgcgatt ttgtgtctg atttctgtt tttaagtcg 
      tatccgtgt ctgggcgat gttgggttc gtgttgagt 
      cttttgccc tgtttcata cacttgttc aattactcc 
      cagcaggga tttttagtt ggggtgatt tttcttatt 
      acgacagct ttgggtccg attttgtgg catttgtgg 
      atttattca aactccgca aatgcgaac ttttatttc 
      ggagtcact ttggccttt gccattgca cagattttt 
      cttgtgact gatattttg ttcgcgtat ttgaagcgc 
      gagttttgt ttgaatcat ggcaaggag aggaagatt 
      gatggaaag gacgccttg ctgatttta gagtga
    mRNA sequence
    • >TC010108 RA
      tcctgttcc tgtcacgat actaacctc actttattc 
      aattattta gtgggccgg taatcgcca atatttccg 
      aaaactgcc cctgaaaag tgtaaaata atggcacat 
      gggaaaacc gaaaaatgt gagcaagac tgctacaaa 
      ggcggaacc gttttattc atgtacttg acggggctt 
      ggtatccgg tattggctc atgttttcg agatatcag 
      tccataatt gcgaaccac atcgaaata tctacacct 
      ctgaactcc tggaagcga gtgtccgaa gggctttgt 
      ctccagtcc aaaggtatc aatccatac gaaggggac 
      ttacttcat gaagctccg ataacaata ttattttat 
      aaagtgttg acacagtgg tggaagttg gacattaag 
      actattttt ctcgccttt gatgcggga acggccgtg 
      gtattgtat ttcgtcgcg aagaaatac atgtttcac 
      ttatttatt gaacaagaa gagaataaa aaacaatat 
      gctctgaac agtaaggag ttcctgtta tcgggctct 
      gatttcgtc aattcgccc gtttttgtc gcatcggcg 
      tttctgttt aatccatac acgattttt aactgcgtt 
      ggacagtca actgtcgtg ttccataat tttttcatc 
      gctttgttt ttgttctgt atgttaagc ggtagcgtt 
      attttgagt gtactgtct ctggcaata tgcacaatg 
      atgtctttc taccccatt gtgttaatt atcccactg 
      tttttgtac tctaacgga atttacaaa agcaaattt 
      cgggcgatt ctcaccccg attttgttt atcgccaca 
      cttgcggct tttataacc atttcgatt ggtttaagc 
      aacggaacc gcatttatg aaaaacgtg tacggtttc 
      attctaaca gttccagac ttgcagcca aatattggc 
      ttgttttgg tattttttc accgaaatg ttcgaccac 
      tttcgcgac ttgttcatc agctcgttt caaataaac 
      gcaaccgtt ttgtattta attccactg tcggtcaaa 
      tttcgcaaa gacccgttt ttgctaacg ttcgcgatt 
      ttgtgtctg atttctgtt tttaagtcg tatccgtgt 
      ctgggcgat gttgggttc gtgttgagt cttttgccc 
      tgtttcata cacttgttc aattactcc cagcaggga 
      tttttagtt ggggtgatt tttcttatt acgacagct 
      ttgggtccg attttgtgg catttgtgg atttattca 
      aactccgca aatgcgaac ttttatttc ggagtcact 
      ttggccttt gccattgca cagattttt cttgtgact 
      gatattttg ttcgcgtat ttgaagcgc gagttttgt 
      ttgaatcat ggcaaggag aggaagatt gatggaaag 
      gacgccttg ctgatttta gagtgattt gtgatctag 
      atgttaacc acaataaat aagactttt gtacaaata 
      ttgttttat ttatttatt atataatta tagacttgt 
      ttgatgatt cgactaaca ttttgaact ggttttcta 
      atccacttt cccttatta gtggattaa acttgatca 
      taattttga ccatctgaa attaaagta gttataaat 
      attgtaata aaaagtaca attcattac atttttaat 
      aatattaat gtatttcct gtcaaaatt taaaagcac 
      cagtaaaag gtaggtcat ctttgacca ttcttgggt 
      taaaacaca ttttcttac aagtcttaa gatctgtgt 
      tggtgagtc ccagaagag gtcaaatgc tgaactttg 
      acgttctat acaatcttg agcctcttt tctcgtaat 
      aagtgtgtt attttctaa acaatgaat aagcaaaag 
      aagttcttt tacacacac ttccggaca ttttgtgtc 
      actgtttcc ttctctcca ctttttggc ctttttttt 
      actgatttt tccttcttt gctcacgtt tcttgtttg 
      tttactcgc aaatcgttt attcttttt cgtgaccac 
      ttctaaatt tcttttctg gagtttttc aaatatttc 
      cacattttc tccgtattc ttcctggtc gactgattc 
      tttccaaat cttttttct ttcctcatc aaggagaat 
      tttttttaa atcaaattt cgtcatttt ttattttaa 
      atacataac ctcaaaaca c
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