Status
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Gene information
General information
TC010102 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 15168443..15178675
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010102
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04760

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    head appendages partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head appendages partially orientation irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head bristle pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: ventral vt to close to dorsal gt
    head not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    legs shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection



    Pupal morphology
    pronotum elongated - Number of animals showing the phenotype: 5

    wings shape irregular - Number of animals showing the phenotype: 4

    abdomen circumference decreased - Number of animals showing the phenotype: 3

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010102 RA
      MAPGTSGGA IPAHHSPYD LRRKSPSHH DGPGPSTSA 
      ATAGSPTSP ATPTAPAQG YTSAMLPAR KRPRRTCSA 
      SYENCTNTA AHYLQYELP DEVLLTIFN YLLEQDLCR 
      VSQVCKRFQ AIANDTEIW KRLYQSVYE YDLPLFNPA 
      PCVFQFISP EESDLANPW KESFRQLYR GIHVRPGYQ 
      DLTFKGRNL VYFNTIQAA LDYADERSG SNSTSGCLL 
      VNNNHTEGT TQGALIFLH AGTYRGEFL VIDDSDIAL 
      IGAAPGNVA ESVILERES ESTVMFVEG AKNAYCGHL 
      TLKFSPDVT STVPHHKHY CLEVGENCS PTIDHCIIR 
      STSVVGAAV CVSGAGANP VIRHCDISD CENVGLYVT 
      DFAQGTYED NEISRNALA GIWVKNNAN PIMRRNHIH 
      HGRDVGIFT FDSGMGYFE ANDIHNNRI AGFEVKAGA 
      NPTVVQCEI HHGQTGGIY VHENGLGQF IDNKIHSNN 
      FAGVWITSN SNPTIRRNE IYNGHQGGV YIFGEGRGL 
      IEHNNIYGN ALAGIQIRT NSDPIVRHN KIHHGQHGG 
      IYVHEKGQG LIEENEVYA NTLAGVWIT TGSSPVLRR 
      NRIHSGKQV GVYFYDNGH GKLEDNDIF NHLYSGVQI 
      RTGSNPVIR GNKIWGGQN GGVLVYNGG LGLLEQNEI 
      FDNAMAGVW IKTDSNPTL KRNKIFDGR DGGICIFNG 
      GKGILEEND IFRNAQAGV LISTQSHPI LRRNRIFDG 
      LAAGVEITN NATATLESN QIFNNRFGG LCLASGVQP 
      IVRGNKIFN NQDAVEKAV ANGQCLYKI SSYTSFPMH 
      DFYRCQTCN TTDRNAICV NCIKTCHAG HDVEFIRHD 
      RFFCDCGAG TLTNQCQLQ GEPTQDTDT LYDSAAPME 
      SHTLMVN
    CDS sequence
    • >TC010102 RA
      atggcccct gggaccagc ggcggcgcc attccagcc 
      catcactct ccttacgac cttcgacgt aagtctccg 
      tcccatcac gacgggccg gggcccagc accagcgcc 
      gcgacggca ggatcgccc acttccccg gcgactccg 
      acggcacca gcacaaggg tacacttcc gccatgctt 
      ccggcgagg aaacgtcca agacggact tgttccgct 
      tcgtacgag aattgtaca aacacagcc gctcattat 
      ctccaatac gagctgccg gacgaagtt ttgctcacg 
      atattcaat tatttactc gaacaagat ttgtgtcgt 
      gtgagtcaa gtgtgtaaa cggtttcag gccattgcg 
      aatgatacc gagatttgg aaacgccta tatcagagt 
      gtctatgaa tacgatttg cccttgttt aatccggca 
      ccttgtgtt ttccaattt atcagtccg gaggagtct 
      gatttggct aatccatgg aaggagtct ttccgtcag 
      ttgtatcgc gggatacac gttcgacca ggttatcaa 
      gacttgacg ttcaaagga cgcaatctt gtgtacttt 
      aatacgata caagcggct ttagattat gccgatgaa 
      cgtagtggt agtaatagt acgagcggt tgtctcttg 
      gtcaataat aatcacact gagggtacc actcaaggc 
      gcactcata tttctacat gccggtact tatcgtgga 
      gagttcctc gtaatcgac gactcagac attgctctt 
      ataggtgcg gcacctggg aacgtggcc gaatccgtg 
      atactagaa cgagaatcc gaatcgacg gtgatgttc 
      gtcgaaggt gcaaaaaac gcctactgt ggccatctt 
      acgttgaaa ttttcacct gatgtcaca tctacagta 
      cctcaccat aaacattat tgtctcgaa gttggagaa 
      aattgtagt ccgactata gatcattgc attatacga 
      agcacatca gttgttggt gcggccgtt tgtgtgagt 
      ggtgccggc gcaaatccc gtcatacgt cattgtgat 
      ataagtgat tgtgaaaat gtcggcttg tacgtcacc 
      gattttgcc caaggaacg tatgaagat aacgagatc 
      agtcggaat gcattggct ggtatttgg gtgaaaaat 
      aacgcgaac ccgatcatg cgtcgtaat cacatacac 
      catggaagg gacgtcgga atatttacg ttcgatagt 
      ggaatgggt tatttcgaa gccaacgat attcataat 
      aatcgaatt gccggtttt gaagtgaaa gcaggcgcg 
      aaccccacc gtagtccaa tgtgagatc catcacggc 
      caaaccggc ggtatctac gtccacgag aacggtctc 
      ggtcaattc atcgataat aaaatccat tcgaataat 
      ttcgccgga gtttggatc acatcaaat agcaatccg 
      acgatacgt cgtaacgaa atctacaat gggcatcaa 
      ggtggtgtt tatattttc ggagagggg cgcggttta 
      atcgaacac aataacatc tacggtaac gcattagct 
      ggcattcaa ataaggact aacagtgac cccatcgtg 
      cgtcacaat aaaatccat cacggccaa cacggcggc 
      atctacgtt cacgaaaaa ggtcaaggc ttaatcgag 
      gagaacgaa gtgtacgca aacacacta gctggggtg 
      tggataaca acggggagt tcaccggtt ttgagacga 
      aatcggatc cattccggc aaacaagtc ggcgtttat 
      ttctatgat aatggtcac ggaaagctc gaagataac 
      gatattttc aatcacctt tattcgggc gtccagatc 
      cggactggg agcaatccg gtgattcgc ggaaacaag 
      atttggggc ggacaaaat gggggcgtt ttggtctac 
      aacggcggg ctcggtttg ttggaacag aacgagatt 
      tttgacaat gccatggcc ggagtttgg atcaagacc 
      gattcaaac cccacactt aaacgtaat aagattttt 
      gacggtcgt gacggagga atttgtatt tttaacggt 
      gggaaaggg attttggaa gagaacgat attttccgg 
      aatgcgcaa gctggtgtg ctcatttcg acgcagagt 
      catccgatt ttgagacgg aatcggatt ttcgacggt 
      ttggccgca ggcgtggaa attacgaat aacgcaacg 
      gccacactt gaatctaac cagattttt aataatcgg 
      ttcgggggc ttgtgtctg gcgagcggt gtgcaacca 
      atagtgaga gggaataag atttttaat aatcaggat 
      gcggtggag aaggctgta gcgaatgga cagtgtctc 
      tacaagatt tcgagttat acgtcgttt ccgatgcac 
      gatttttac aggtgtcag acttgtaat acgacggac 
      aggaatgcg atctgtgtg aattgcatc aagacgtgt 
      catgcagga catgatgtg gagtttatc agacatgac 
      agattcttc tgcgattgt ggggctggt acgttaaca 
      aatcagtgt cagttgcaa ggagagcca acccaagac 
      acggacact ctgtacgat tcggctgct cctatggaa 
      tctcacacc ttgatggtc aactaa
    mRNA sequence
    • >TC010102 RA
      cggagggca tccgtcgca agcctactt gattttgtt 
      catgttgtg tgaaaaggt gtttttcgt gtccttatc 
      ctgaaaggt gaatggatt acctgttgt gcggcgact 
      tccagacgt gtaaactgg cgacataag tcaccgaca 
      tcagcaagc atagcccga tggaacgaa aacaatcaa 
      aaattccat agaaatcct gcaccattc tgacatttt 
      aacggtcac gtgacgtca aaccagccc ggtgattgg 
      ttggtccgg ttccgtggt tgtcggaac ggtagcgaa 
      tatggccgc cagtgctag ttccagtgt ctcctttgt 
      ttagagatt ggttttggt gctagtgct agaaaaaat 
      gccgagcgc ctctttcac ggcctccag gtcgtacgt 
      aaggaggtc ccgcaggaa gggcgctaa caggatccc 
      gactccttc caggaccac gtctgcaga gccatgcga 
      accttcctg tcctgtacc taaccaaaa cattccagc 
      acctacggc ggccgctcc gggctctgg gggggcctt 
      cagcaatgg cccctggga ccagcggcg gcgccattc 
      cagcccatc actctcctt acgaccttc gacgtaagt 
      ctccgtccc atcacgacg ggccggggc ccagcacca 
      gcgccgcga cggcaggat cgcccactt ccccggcga 
      ctccgacgg caccagcac aagggtaca cttccgcca 
      tgcttccgg cgaggaaac gtccaagac ggacttgtt 
      ccgcttcgt acgagaatt gtacaaaca cagccgctc 
      attatctcc aatacgagc tgccggacg aagttttgc 
      tcacgatat tcaattatt tactcgaac aagatttgt 
      gtcgtgtga gtcaagtgt gtaaacggt ttcaggcca 
      ttgcgaatg ataccgaga tttggaaac gcctatatc 
      agagtgtct atgaatacg atttgccct tgtttaatc 
      cggcacctt gtgttttcc aatttatca gtccggagg 
      agtctgatt tggctaatc catggaagg agtctttcc 
      gtcagttgt atcgcggga tacacgttc gaccaggtt 
      atcaagact tgacgttca aaggacgca atcttgtgt 
      actttaata cgatacaag cggctttag attatgccg 
      atgaacgta gtggtagta atagtacga gcggttgtc 
      tcttggtca ataataatc acactgagg gtaccactc 
      aaggcgcac tcatatttc tacatgccg gtacttatc 
      gtggagagt tcctcgtaa tcgacgact cagacattg 
      ctcttatag gtgcggcac ctgggaacg tggccgaat 
      ccgtgatac tagaacgag aatccgaat cgacggtga 
      tgttcgtcg aaggtgcaa aaaacgcct actgtggcc 
      atcttacgt tgaaatttt cacctgatg tcacatcta 
      cagtacctc accataaac attattgtc tcgaagttg 
      gagaaaatt gtagtccga ctatagatc attgcatta 
      tacgaagca catcagttg ttggtgcgg ccgtttgtg 
      tgagtggtg ccggcgcaa atcccgtca tacgtcatt 
      gtgatataa gtgattgtg aaaatgtcg gcttgtacg 
      tcaccgatt ttgcccaag gaacgtatg aagataacg 
      agatcagtc ggaatgcat tggctggta tttgggtga 
      aaaataacg cgaacccga tcatgcgtc gtaatcaca 
      tacaccatg gaagggacg tcggaatat ttacgttcg 
      atagtggaa tgggttatt tcgaagcca acgatattc 
      ataataatc gaattgccg gttttgaag tgaaagcag 
      gcgcgaacc ccaccgtag tccaatgtg agatccatc 
      acggccaaa ccggcggta tctacgtcc acgagaacg 
      gtctcggtc aattcatcg ataataaaa tccattcga 
      ataatttcg ccggagttt ggatcacat caaatagca 
      atccgacga tacgtcgta acgaaatct acaatgggc 
      atcaaggtg gtgtttata ttttcggag aggggcgcg 
      gtttaatcg aacacaata acatctacg gtaacgcat 
      tagctggca ttcaaataa ggactaaca gtgacccca 
      tcgtgcgtc acaataaaa tccatcacg gccaacacg 
      gcggcatct acgttcacg aaaaaggtc aaggcttaa 
      tcgaggaga acgaagtgt acgcaaaca cactagctg 
      gggtgtgga taacaacgg ggagttcac cggttttga 
      gacgaaatc ggatccatt ccggcaaac aagtcggcg 
      tttatttct atgataatg gtcacggaa agctcgaag 
      ataacgata ttttcaatc acctttatt cgggcgtcc 
      agatccgga ctgggagca atccggtga ttcgcggaa 
      acaagattt ggggcggac aaaatgggg gcgttttgg 
      tctacaacg gcgggctcg gtttgttgg aacagaacg 
      agatttttg acaatgcca tggccggag tttggatca 
      agaccgatt caaacccca cacttaaac gtaataaga 
      tttttgacg gtcgtgacg gaggaattt gtattttta 
      acggtggga aagggattt tggaagaga acgatattt 
      tccggaatg cgcaagctg gtgtgctca tttcgacgc 
      agagtcatc cgattttga gacggaatc ggattttcg 
      acggtttgg ccgcaggcg tggaaatta cgaataacg 
      caacggcca cacttgaat ctaaccaga tttttaata 
      atcggttcg ggggcttgt gtctggcga gcggtgtgc 
      aaccaatag tgagaggga ataagattt ttaataatc 
      aggatgcgg tggagaagg ctgtagcga atggacagt 
      gtctctaca agatttcga gttatacgt cgtttccga 
      tgcacgatt tttacaggt gtcagactt gtaatacga 
      cggacagga atgcgatct gtgtgaatt gcatcaaga 
      cgtgtcatg caggacatg atgtggagt ttatcagac 
      atgacagat tcttctgcg attgtgggg ctggtacgt 
      taacaaatc agtgtcagt tgcaaggag agccaaccc 
      aagacacgg acactctgt acgattcgg ctgctccta 
      tggaatctc acaccttga tggtcaact aaggttgtc 
      gtctctcta attgttttt taatcgccg tactaattt 
      atttcaaat cgacgaaag tgttcagaa acagtgttt 
      attactttc taacagacg aaatattca atattgtgt 
      ttttttaat gcacaaatt catgtttcg gtgtatgag 
      aaatgctta gcaatatat ttacagcag tttacacac 
      catttttag agttagaca atcaacaca ggcgcttcc 
      aaagaccaa aattttgtt tgttttttt cagaaatta 
      aggattgtt tttaaattc atcgtgttt tgttgttta 
      ccaaaggta acgaatggc acaattata cattctttt 
      ttgtattta ttcaatgat attggtaat aaaagtagt 
      tttttgttt aatgttttt tttgaatct caaagatta 
      aaaggatta tgcaccaca ttttaagca atatggtta 
      atttctttc acggtttgg tcagtcacg aatacgtta 
      tttttttga atagaaaaa gttataaca attatgatg 
      attaataag attttgtac aggttattg ttagaattt 
      gaaaaatat tgctgttta tgtttaagt ttatactta 
      agttaatta ctgttatta ttttttaat tatttattt 
      tatttagtt tagcgtaca agaatggat atgcggtct 
      taatgactg ttgtatgaa tttttgttt tctccatat 
      tgtattagt gaagatagc tttaaataa tatctgatg 
      taaataagg ttgtgcgga gacaagttg agattcaga 
      gtgttgtgc ggatgacga aaagttgtg tttatataa 
      aaacgaaac aacgttgtt gcatgtgtg acaaataat 
      gtaaaaaat cagtattta aattcgcgt ttgtttttt 
      aggaaaatt ggttgtttg atagaaatg tgtaagtta 
      aatgtgtat gtacgttgt atataataa ttattacaa 
      aaatgcaat caataattt aaccgagag ctttcaaga 
      tatgatttc acaatttca taacaggcc gaccaatct 
      tttgaacgt ttaaaaaac tcaaatggg acgcctgta 
      ctgttatat attgtatag gtacaataa ataaagtgc 
      agtttttaa actggtgcc atattcaca tttacacat 
      ttatcaagt agttggaag gaataccca tagatttaa 
      ttatgtaag acatagtgt aattctttt tataccatt 
      ttcttacgt tgactgatg tatacatag tgacaaaaa 
      ctttattta actcttgcc acggttggg tgaaaacgt 
      tgggtgcaa cgatgaacg gtattgtga tttttttct 
      aggtagacg gcgaaagtt acacgaatt ccagttatt 
      tttaagagt gactctttc caattgttt ttttattta 
      tagttgttt gtggaagag ggttttgat tttttatac 
      aaaaatcgc tcagatttg taaattaaa aactactca 
      atacaaccc tacttatat a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY