Status
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Gene information
General information
TC010102 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 15168443..15178675
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC010102
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04760

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     head appendages partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head appendages partially orientation irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head bristle pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: ventral vt to close to dorsal gt
    head not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    legs shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    Pupal morphology
    pronotum elongated - Number of animals showing the phenotype: 5

    wings shape irregular - Number of animals showing the phenotype: 4

    abdomen circumference decreased - Number of animals showing the phenotype: 3

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010102 RA
      MAPGTSGGA IPAHHSPYD LRRKSPSHH DGPGPSTSA 
ATAGSPTSP ATPTAPAQG YTSAMLPAR KRPRRTCSA 
SYENCTNTA AHYLQYELP DEVLLTIFN YLLEQDLCR 
VSQVCKRFQ AIANDTEIW KRLYQSVYE YDLPLFNPA 
PCVFQFISP EESDLANPW KESFRQLYR GIHVRPGYQ 
DLTFKGRNL VYFNTIQAA LDYADERSG SNSTSGCLL 
VNNNHTEGT TQGALIFLH AGTYRGEFL VIDDSDIAL 
IGAAPGNVA ESVILERES ESTVMFVEG AKNAYCGHL 
TLKFSPDVT STVPHHKHY CLEVGENCS PTIDHCIIR 
STSVVGAAV CVSGAGANP VIRHCDISD CENVGLYVT 
DFAQGTYED NEISRNALA GIWVKNNAN PIMRRNHIH 
HGRDVGIFT FDSGMGYFE ANDIHNNRI AGFEVKAGA 
NPTVVQCEI HHGQTGGIY VHENGLGQF IDNKIHSNN 
FAGVWITSN SNPTIRRNE IYNGHQGGV YIFGEGRGL 
IEHNNIYGN ALAGIQIRT NSDPIVRHN KIHHGQHGG 
IYVHEKGQG LIEENEVYA NTLAGVWIT TGSSPVLRR 
NRIHSGKQV GVYFYDNGH GKLEDNDIF NHLYSGVQI 
RTGSNPVIR GNKIWGGQN GGVLVYNGG LGLLEQNEI 
FDNAMAGVW IKTDSNPTL KRNKIFDGR DGGICIFNG 
GKGILEEND IFRNAQAGV LISTQSHPI LRRNRIFDG 
LAAGVEITN NATATLESN QIFNNRFGG LCLASGVQP 
IVRGNKIFN NQDAVEKAV ANGQCLYKI SSYTSFPMH 
DFYRCQTCN TTDRNAICV NCIKTCHAG HDVEFIRHD 
RFFCDCGAG TLTNQCQLQ GEPTQDTDT LYDSAAPME 
SHTLMVN
    CDS sequence
    • >TC010102 RA
      atggcccct gggaccagc ggcggcgcc attccagcc 
catcactct ccttacgac cttcgacgt aagtctccg 
tcccatcac gacgggccg gggcccagc accagcgcc 
gcgacggca ggatcgccc acttccccg gcgactccg 
acggcacca gcacaaggg tacacttcc gccatgctt 
ccggcgagg aaacgtcca agacggact tgttccgct 
tcgtacgag aattgtaca aacacagcc gctcattat 
ctccaatac gagctgccg gacgaagtt ttgctcacg 
atattcaat tatttactc gaacaagat ttgtgtcgt 
gtgagtcaa gtgtgtaaa cggtttcag gccattgcg 
aatgatacc gagatttgg aaacgccta tatcagagt 
gtctatgaa tacgatttg cccttgttt aatccggca 
ccttgtgtt ttccaattt atcagtccg gaggagtct 
gatttggct aatccatgg aaggagtct ttccgtcag 
ttgtatcgc gggatacac gttcgacca ggttatcaa 
gacttgacg ttcaaagga cgcaatctt gtgtacttt 
aatacgata caagcggct ttagattat gccgatgaa 
cgtagtggt agtaatagt acgagcggt tgtctcttg 
gtcaataat aatcacact gagggtacc actcaaggc 
gcactcata tttctacat gccggtact tatcgtgga 
gagttcctc gtaatcgac gactcagac attgctctt 
ataggtgcg gcacctggg aacgtggcc gaatccgtg 
atactagaa cgagaatcc gaatcgacg gtgatgttc 
gtcgaaggt gcaaaaaac gcctactgt ggccatctt 
acgttgaaa ttttcacct gatgtcaca tctacagta 
cctcaccat aaacattat tgtctcgaa gttggagaa 
aattgtagt ccgactata gatcattgc attatacga 
agcacatca gttgttggt gcggccgtt tgtgtgagt 
ggtgccggc gcaaatccc gtcatacgt cattgtgat 
ataagtgat tgtgaaaat gtcggcttg tacgtcacc 
gattttgcc caaggaacg tatgaagat aacgagatc 
agtcggaat gcattggct ggtatttgg gtgaaaaat 
aacgcgaac ccgatcatg cgtcgtaat cacatacac 
catggaagg gacgtcgga atatttacg ttcgatagt 
ggaatgggt tatttcgaa gccaacgat attcataat 
aatcgaatt gccggtttt gaagtgaaa gcaggcgcg 
aaccccacc gtagtccaa tgtgagatc catcacggc 
caaaccggc ggtatctac gtccacgag aacggtctc 
ggtcaattc atcgataat aaaatccat tcgaataat 
ttcgccgga gtttggatc acatcaaat agcaatccg 
acgatacgt cgtaacgaa atctacaat gggcatcaa 
ggtggtgtt tatattttc ggagagggg cgcggttta 
atcgaacac aataacatc tacggtaac gcattagct 
ggcattcaa ataaggact aacagtgac cccatcgtg 
cgtcacaat aaaatccat cacggccaa cacggcggc 
atctacgtt cacgaaaaa ggtcaaggc ttaatcgag 
gagaacgaa gtgtacgca aacacacta gctggggtg 
tggataaca acggggagt tcaccggtt ttgagacga 
aatcggatc cattccggc aaacaagtc ggcgtttat 
ttctatgat aatggtcac ggaaagctc gaagataac 
gatattttc aatcacctt tattcgggc gtccagatc 
cggactggg agcaatccg gtgattcgc ggaaacaag 
atttggggc ggacaaaat gggggcgtt ttggtctac 
aacggcggg ctcggtttg ttggaacag aacgagatt 
tttgacaat gccatggcc ggagtttgg atcaagacc 
gattcaaac cccacactt aaacgtaat aagattttt 
gacggtcgt gacggagga atttgtatt tttaacggt 
gggaaaggg attttggaa gagaacgat attttccgg 
aatgcgcaa gctggtgtg ctcatttcg acgcagagt 
catccgatt ttgagacgg aatcggatt ttcgacggt 
ttggccgca ggcgtggaa attacgaat aacgcaacg 
gccacactt gaatctaac cagattttt aataatcgg 
ttcgggggc ttgtgtctg gcgagcggt gtgcaacca 
atagtgaga gggaataag atttttaat aatcaggat 
gcggtggag aaggctgta gcgaatgga cagtgtctc 
tacaagatt tcgagttat acgtcgttt ccgatgcac 
gatttttac aggtgtcag acttgtaat acgacggac 
aggaatgcg atctgtgtg aattgcatc aagacgtgt 
catgcagga catgatgtg gagtttatc agacatgac 
agattcttc tgcgattgt ggggctggt acgttaaca 
aatcagtgt cagttgcaa ggagagcca acccaagac 
acggacact ctgtacgat tcggctgct cctatggaa 
tctcacacc ttgatggtc aactaa
    mRNA sequence
    • >TC010102 RA
      cggagggca tccgtcgca agcctactt gattttgtt 
catgttgtg tgaaaaggt gtttttcgt gtccttatc 
ctgaaaggt gaatggatt acctgttgt gcggcgact 
tccagacgt gtaaactgg cgacataag tcaccgaca 
tcagcaagc atagcccga tggaacgaa aacaatcaa 
aaattccat agaaatcct gcaccattc tgacatttt 
aacggtcac gtgacgtca aaccagccc ggtgattgg 
ttggtccgg ttccgtggt tgtcggaac ggtagcgaa 
tatggccgc cagtgctag ttccagtgt ctcctttgt 
ttagagatt ggttttggt gctagtgct agaaaaaat 
gccgagcgc ctctttcac ggcctccag gtcgtacgt 
aaggaggtc ccgcaggaa gggcgctaa caggatccc 
gactccttc caggaccac gtctgcaga gccatgcga 
accttcctg tcctgtacc taaccaaaa cattccagc 
acctacggc ggccgctcc gggctctgg gggggcctt 
cagcaatgg cccctggga ccagcggcg gcgccattc 
cagcccatc actctcctt acgaccttc gacgtaagt 
ctccgtccc atcacgacg ggccggggc ccagcacca 
gcgccgcga cggcaggat cgcccactt ccccggcga 
ctccgacgg caccagcac aagggtaca cttccgcca 
tgcttccgg cgaggaaac gtccaagac ggacttgtt 
ccgcttcgt acgagaatt gtacaaaca cagccgctc 
attatctcc aatacgagc tgccggacg aagttttgc 
tcacgatat tcaattatt tactcgaac aagatttgt 
gtcgtgtga gtcaagtgt gtaaacggt ttcaggcca 
ttgcgaatg ataccgaga tttggaaac gcctatatc 
agagtgtct atgaatacg atttgccct tgtttaatc 
cggcacctt gtgttttcc aatttatca gtccggagg 
agtctgatt tggctaatc catggaagg agtctttcc 
gtcagttgt atcgcggga tacacgttc gaccaggtt 
atcaagact tgacgttca aaggacgca atcttgtgt 
actttaata cgatacaag cggctttag attatgccg 
atgaacgta gtggtagta atagtacga gcggttgtc 
tcttggtca ataataatc acactgagg gtaccactc 
aaggcgcac tcatatttc tacatgccg gtacttatc 
gtggagagt tcctcgtaa tcgacgact cagacattg 
ctcttatag gtgcggcac ctgggaacg tggccgaat 
ccgtgatac tagaacgag aatccgaat cgacggtga 
tgttcgtcg aaggtgcaa aaaacgcct actgtggcc 
atcttacgt tgaaatttt cacctgatg tcacatcta 
cagtacctc accataaac attattgtc tcgaagttg 
gagaaaatt gtagtccga ctatagatc attgcatta 
tacgaagca catcagttg ttggtgcgg ccgtttgtg 
tgagtggtg ccggcgcaa atcccgtca tacgtcatt 
gtgatataa gtgattgtg aaaatgtcg gcttgtacg 
tcaccgatt ttgcccaag gaacgtatg aagataacg 
agatcagtc ggaatgcat tggctggta tttgggtga 
aaaataacg cgaacccga tcatgcgtc gtaatcaca 
tacaccatg gaagggacg tcggaatat ttacgttcg 
atagtggaa tgggttatt tcgaagcca acgatattc 
ataataatc gaattgccg gttttgaag tgaaagcag 
gcgcgaacc ccaccgtag tccaatgtg agatccatc 
acggccaaa ccggcggta tctacgtcc acgagaacg 
gtctcggtc aattcatcg ataataaaa tccattcga 
ataatttcg ccggagttt ggatcacat caaatagca 
atccgacga tacgtcgta acgaaatct acaatgggc 
atcaaggtg gtgtttata ttttcggag aggggcgcg 
gtttaatcg aacacaata acatctacg gtaacgcat 
tagctggca ttcaaataa ggactaaca gtgacccca 
tcgtgcgtc acaataaaa tccatcacg gccaacacg 
gcggcatct acgttcacg aaaaaggtc aaggcttaa 
tcgaggaga acgaagtgt acgcaaaca cactagctg 
gggtgtgga taacaacgg ggagttcac cggttttga 
gacgaaatc ggatccatt ccggcaaac aagtcggcg 
tttatttct atgataatg gtcacggaa agctcgaag 
ataacgata ttttcaatc acctttatt cgggcgtcc 
agatccgga ctgggagca atccggtga ttcgcggaa 
acaagattt ggggcggac aaaatgggg gcgttttgg 
tctacaacg gcgggctcg gtttgttgg aacagaacg 
agatttttg acaatgcca tggccggag tttggatca 
agaccgatt caaacccca cacttaaac gtaataaga 
tttttgacg gtcgtgacg gaggaattt gtattttta 
acggtggga aagggattt tggaagaga acgatattt 
tccggaatg cgcaagctg gtgtgctca tttcgacgc 
agagtcatc cgattttga gacggaatc ggattttcg 
acggtttgg ccgcaggcg tggaaatta cgaataacg 
caacggcca cacttgaat ctaaccaga tttttaata 
atcggttcg ggggcttgt gtctggcga gcggtgtgc 
aaccaatag tgagaggga ataagattt ttaataatc 
aggatgcgg tggagaagg ctgtagcga atggacagt 
gtctctaca agatttcga gttatacgt cgtttccga 
tgcacgatt tttacaggt gtcagactt gtaatacga 
cggacagga atgcgatct gtgtgaatt gcatcaaga 
cgtgtcatg caggacatg atgtggagt ttatcagac 
atgacagat tcttctgcg attgtgggg ctggtacgt 
taacaaatc agtgtcagt tgcaaggag agccaaccc 
aagacacgg acactctgt acgattcgg ctgctccta 
tggaatctc acaccttga tggtcaact aaggttgtc 
gtctctcta attgttttt taatcgccg tactaattt 
atttcaaat cgacgaaag tgttcagaa acagtgttt 
attactttc taacagacg aaatattca atattgtgt 
ttttttaat gcacaaatt catgtttcg gtgtatgag 
aaatgctta gcaatatat ttacagcag tttacacac 
catttttag agttagaca atcaacaca ggcgcttcc 
aaagaccaa aattttgtt tgttttttt cagaaatta 
aggattgtt tttaaattc atcgtgttt tgttgttta 
ccaaaggta acgaatggc acaattata cattctttt 
ttgtattta ttcaatgat attggtaat aaaagtagt 
tttttgttt aatgttttt tttgaatct caaagatta 
aaaggatta tgcaccaca ttttaagca atatggtta 
atttctttc acggtttgg tcagtcacg aatacgtta 
tttttttga atagaaaaa gttataaca attatgatg 
attaataag attttgtac aggttattg ttagaattt 
gaaaaatat tgctgttta tgtttaagt ttatactta 
agttaatta ctgttatta ttttttaat tatttattt 
tatttagtt tagcgtaca agaatggat atgcggtct 
taatgactg ttgtatgaa tttttgttt tctccatat 
tgtattagt gaagatagc tttaaataa tatctgatg 
taaataagg ttgtgcgga gacaagttg agattcaga 
gtgttgtgc ggatgacga aaagttgtg tttatataa 
aaacgaaac aacgttgtt gcatgtgtg acaaataat 
gtaaaaaat cagtattta aattcgcgt ttgtttttt 
aggaaaatt ggttgtttg atagaaatg tgtaagtta 
aatgtgtat gtacgttgt atataataa ttattacaa 
aaatgcaat caataattt aaccgagag ctttcaaga 
tatgatttc acaatttca taacaggcc gaccaatct 
tttgaacgt ttaaaaaac tcaaatggg acgcctgta 
ctgttatat attgtatag gtacaataa ataaagtgc 
agtttttaa actggtgcc atattcaca tttacacat 
ttatcaagt agttggaag gaataccca tagatttaa 
ttatgtaag acatagtgt aattctttt tataccatt 
ttcttacgt tgactgatg tatacatag tgacaaaaa 
ctttattta actcttgcc acggttggg tgaaaacgt 
tgggtgcaa cgatgaacg gtattgtga tttttttct 
aggtagacg gcgaaagtt acacgaatt ccagttatt 
tttaagagt gactctttc caattgttt ttttattta 
tagttgttt gtggaagag ggttttgat tttttatac 
aaaaatcgc tcagatttg taaattaaa aactactca 
atacaaccc tacttatat a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY