Status
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Gene information
General information
TC010099 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 15202786..15222445
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010099
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04759

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010099 RA
      MILIVLVFV ACVNWTLAQ CPEGITGNC TIRHNETSP 
      KPPLLQAMN MLAEDDDHK GRALNFTMT ESNNTHDEH 
      GDLSDVTIN EDEEEHDKI TAHNVTVCS KGGLTYEEG 
      EKLEIGCES TCTCRNGKM QCEDRCVKP MVRKGKKIE 
      DPLCIEKDT DDECCAVLV CTADTETEP LEICTFKNH 
      TYTRGESFN KGCDEVCTC EIAGKTSCK PRCPPVNKT 
      NDKCVEVQD PNDSCCKKL LCDVTLDDH EADKDYSMQ 
      MHKLIEAKY LNESTVELQ FDPKLNEKE NSIFIEGSA 
      DQNDWKTYK IRAQKYVDV MDGTKYLKL ENSKESVKI 
      ESAATKTPL ENASSSACT YKNKSFELN EEYNDNCTS 
      LCVCKESGM KCLKLECPT YFGVDVLDP NCIEWETVP 
      PNFHPTPPN CCPQELKCK SNGSCVYEG HTYQNWQQL 
      PENVTGCEK RCYCEMGNV ECQNTCPPV TALPPPTLP 
      CPPHQAIVD HLPDDECCM YWVCSQESG RNETKMNAT 
      EIKTPSKIM GPLLVYKNH NKTDNKNPL EPFTPPNLP 
      QDENPYYHK NEKDKFLGP YNPNYKQNK KPGLGNNQD 
      ELLQFIHQH PEITNYPSG SVVEIHKPS FNNRPQHFI 
      PYVIPQNGH SDDLPPGIT LEQILQEVH KNANPNGHI 
      LPFPNHFTN GPVLLAPQS PILPRHNAT FPGQQFPGG 
      FPTMQQDDI SVTALEAVD AHTVRLAFV VPSVIVGLH 
      GRVEVRYTH HDDSDVNSW QLQVFAPPN DLIATPQLE 
      FDLLDLKPD TEYKIKITI TLRDLHNTP SSRIYKIRT 
      LKDLNATTL PPMIPIEPE LAISDINAT WVTVIWRKF 
      TEYELQFID GVQLRFKEI EGKIYDATP LIHRSVTSY 
      TLENLKPNT KYEIGIFFI PFPGQLTEL HAEHMLHFT 
      TANEIDTYG FNVTLDISH IKSTSVEIS WSGVPYPED 
      KYVNIYRAI YQSDSGKED HSTFKIAKR DSPTKTVIS 
      DLKPGTRYR LWLEVYLTN GKIKTSNVQ DFITKPRAA 
      PALGAATQS DKLSAGEQK GDYYGPLVI VAILAAIAI 
      MSTLILLLI LVRRHNQNK AAITPPPPR VSQSAYDNP 
      TYKVEIQQE TMGL
    CDS sequence
    • >TC010099 RA
      atgatactc atcgttctg gtgtttgtg gcgtgtgtg 
      aattggact ttagcacag tgtcccgaa ggaataacc 
      gggaattgt accatccga cataatgaa acgtcgccg 
      aaaccgccc ctgctccaa gcaatgaac atgctcgct 
      gaagatgac gaccacaaa gggcgagct cttaatttt 
      acaatgacc gaatcgaac aacacacat gacgaacat 
      ggagacctt agcgatgtc accatcaac gaagatgaa 
      gaagaacat gacaaaatc acagctcat aatgtcacc 
      gtttgctca aaaggaggt ttgacgtac gaagaagga 
      gaaaaatta gaaatcggc tgcgaatcg acgtgcacg 
      tgccgaaac gggaagatg caatgtgaa gatagatgt 
      gttaaaccg atggtgagg aaagggaag aaaattgaa 
      gatccactc tgcattgag aaggacaca gacgacgaa 
      tgttgcgcc gttttggtg tgcacagca gatacggaa 
      acggagcct ttagaaatc tgtacgttc aagaaccac 
      acttacact cgtggcgaa tcgttcaat aaaggctgc 
      gatgaagtt tgcacttgt gaaatcgca ggaaaaaca 
      tcctgcaaa ccgcgatgt cctcctgtt aataaaacg 
      aacgataaa tgtgtggaa gtgcaagac ccgaatgat 
      tcctgttgt aagaaattg ctttgcgat gtgactttg 
      gacgatcac gaagcggac aaggattat tccatgcaa 
      atgcataaa ttaatagaa gctaaatat ttaaatgaa 
      tcgacggtt gaattacaa tttgatccg aagctgaac 
      gagaaagag aactcgatt tttattgaa ggtagtgcc 
      gatcagaac gattggaaa acttataaa ataagagca 
      cagaaatac gtcgatgtt atggacgga actaaatat 
      ctcaaacta gaaaacagc aaagaaagt gttaaaatc 
      gaatcggct gcgacaaaa actccgctc gaaaacgct 
      tcctcatca gcttgcact tacaaaaac aagtctttt 
      gaattaaac gaggagtac aacgacaac tgtacgtct 
      ctttgcgtt tgtaaagag tcaggaatg aagtgtttg 
      aaacttgaa tgtcctact tatttcggc gtggacgtt 
      ctcgatccg aattgtatt gaatgggag acggttcct 
      ccgaatttc catccgacg cctccaaac tgttgtccg 
      caagagttg aaatgtaaa agcaacggt tcttgcgtc 
      tatgaaggt cacacttac caaaactgg cagcaattg 
      cctgagaac gtcaccggt tgcgaaaaa cgatgttat 
      tgcgaaatg ggaaatgtc gaatgtcaa aatacttgt 
      cctccggtg acggcctta ccgccccca acacttccc 
      tgtccgccg catcaagca atagtggat catctgcca 
      gatgacgag tgttgcatg tattgggtg tgcagtcaa 
      gagagtgga cgtaacgaa acaaagatg aacgcaacg 
      gaaataaaa acaccgtcg aaaatcatg ggaccgtta 
      ctagtttac aaaaaccat aacaaaacc gacaataaa 
      aatcctctc gaaccgttc acacctccg aatttgccc 
      caagacgaa aatccatac tatcacaaa aacgaaaaa 
      gacaagttt ctcggtcct tataatcca aattataaa 
      caaaacaag aaacctgga cttggaaat aaccaagac 
      gagttgtta caattcatt caccaacat cccgaaatc 
      acgaattat ccttcggga tccgttgtc gaaattcac 
      aaaccctcc ttcaataat cgcccccaa catttcatc 
      ccctatgtc atcccacag aacggtcat tcggacgac 
      ttaccacct ggcataacc ctcgaacaa attttgcaa 
      gaagtgcat aaaaacgca aacccgaat ggtcatatt 
      ttacccttc cctaatcat ttcactaac ggaccggta 
      cttttagcc ccacaaagt ccaattttg cctagacat 
      aacgcaacg tttccaggc cagcagttt ccagggggt 
      ttccccacg atgcaacag gatgatatt agtgtgacg 
      gcacttgaa gccgtggac gctcacacc gtgcgtttg 
      gcttttgtg gtcccttcg gtcattgtg ggcttgcat 
      ggacgagtg gaagtgcgc tacactcat cacgacgat 
      tcggacgta aattcgtgg caattgcaa gttttcgca 
      cctcccaat gacttgatc gccactcct caactcgaa 
      ttcgacctt ttagacctc aaacctgac accgaatac 
      aagatcaaa ataacgata acgttgcgc gacctccac 
      aacacgcca agtagccga atatacaaa ataagaact 
      ctgaaggat ttaaatgca acaactttg cctccgatg 
      atcccaatc gagcccgaa ttggccatc agcgacata 
      aatgccact tgggtcaca gtgatttgg cgcaaattc 
      accgaatac gaactgcaa ttcatcgat ggagttcag 
      ttacgattc aaggaaatt gagggaaaa atctacgac 
      gctacgcca cttatccac agatctgtg acaagttac 
      acactcgag aacctcaaa cctaacacg aaatacgaa 
      atcgggatt ttcttcatt ccatttccg ggacagttg 
      acggaacta cacgccgaa catatgctg cattttacc 
      acagccaac gaaatcgat acttacggc ttcaatgtt 
      actctggat atctctcat atcaagtca acaagtgtt 
      gagatttcc tggtccgga gtgccgtac ccggaagac 
      aaatacgtc aatatctac cgggcgatt taccaaagc 
      gattccggg aaggaggat catagcacg tttaaaatc 
      gccaagcgg gattccccc acgaaaacg gtcataagt 
      gacctaaaa ccgggcacg agatatcgc ttgtggttg 
      gaggtttac ctcaccaat ggtaaaatt aagacgagt 
      aatgttcaa gactttata acgaagccg agggcggcg 
      ccggcttta ggggcggcc acccaatcg gataaatta 
      tcagcaggg gagcaaaag ggcgactat tatgggccc 
      ctagtcatt gttgctatc ctagcggcg attgccatt 
      atgagtacg ttgattttg ttgctgatt ttagttagg 
      agacacaat cagaataaa gctgccatt acgccgccc 
      ccgccgagg gttagtcaa tcggcctac gataaccca 
      acgtacaaa gtggagatt caacaagag acaatgggg 
      ttatga
    mRNA sequence
    • >TC010099 RA
      gttttagtc ggacttttg ctatccagt ctggttgaa 
      tgaggtgtc attcttttt gattgtgac tgtaggtcg 
      atatacttg cgatagttt gtcaacaca caatacacg 
      ttacatatt aatggagaa tctccgatt ctacatccg 
      gtcgaatat ttcacaatt tgccttgtc atcggggga 
      atttggcaa agaaagtgt gttaaaacg cttggatgt 
      ccaaatgaa gactttcga accacgcat tttttaaac 
      ctttgacca cattgtgcc aatttttat ttttggaat 
      ttttcacct gatgtttac ttgcacctg aaaagggcc 
      atcctacaa tcggtgaac gtcgcccca ctatcacgg 
      ttagtcgct ggtaggctt ggcggaggc caaccatcg 
      ctcatggtt gtacaacag accgctgac cggccgcag 
      acctaccaa gaatttccc agtgatgtg atctaagct 
      gctatttcg agtgcggtg atcgactga cgatcacga 
      tgatactca tcgttctgg tgtttgtgg cgtgtgtga 
      attggactt tagcacagt gtcccgaag gaataaccg 
      ggaattgta ccatccgac ataatgaaa cgtcgccga 
      aaccgcccc tgctccaag caatgaaca tgctcgctg 
      aagatgacg accacaaag ggcgagctc ttaatttta 
      caatgaccg aatcgaaca acacacatg acgaacatg 
      gagacctta gcgatgtca ccatcaacg aagatgaag 
      aagaacatg acaaaatca cagctcata atgtcaccg 
      tttgctcaa aaggaggtt tgacgtacg aagaaggag 
      aaaaattag aaatcggct gcgaatcga cgtgcacgt 
      gccgaaacg ggaagatgc aatgtgaag atagatgtg 
      ttaaaccga tggtgagga aagggaaga aaattgaag 
      atccactct gcattgaga aggacacag acgacgaat 
      gttgcgccg ttttggtgt gcacagcag atacggaaa 
      cggagcctt tagaaatct gtacgttca agaaccaca 
      cttacactc gtggcgaat cgttcaata aaggctgcg 
      atgaagttt gcacttgtg aaatcgcag gaaaaacat 
      cctgcaaac cgcgatgtc ctcctgtta ataaaacga 
      acgataaat gtgtggaag tgcaagacc cgaatgatt 
      cctgttgta agaaattgc tttgcgatg tgactttgg 
      acgatcacg aagcggaca aggattatt ccatgcaaa 
      tgcataaat taatagaag ctaaatatt taaatgaat 
      cgacggttg aattacaat ttgatccga agctgaacg 
      agaaagaga actcgattt ttattgaag gtagtgccg 
      atcagaacg attggaaaa cttataaaa taagagcac 
      agaaatacg tcgatgtta tggacggaa ctaaatatc 
      tcaaactag aaaacagca aagaaagtg ttaaaatcg 
      aatcggctg cgacaaaaa ctccgctcg aaaacgctt 
      cctcatcag cttgcactt acaaaaaca agtcttttg 
      aattaaacg aggagtaca acgacaact gtacgtctc 
      tttgcgttt gtaaagagt caggaatga agtgtttga 
      aacttgaat gtcctactt atttcggcg tggacgttc 
      tcgatccga attgtattg aatgggaga cggttcctc 
      cgaatttcc atccgacgc ctccaaact gttgtccgc 
      aagagttga aatgtaaaa gcaacggtt cttgcgtct 
      atgaaggtc acacttacc aaaactggc agcaattgc 
      ctgagaacg tcaccggtt gcgaaaaac gatgttatt 
      gcgaaatgg gaaatgtcg aatgtcaaa atacttgtc 
      ctccggtga cggccttac cgcccccaa cacttccct 
      gtccgccgc atcaagcaa tagtggatc atctgccag 
      atgacgagt gttgcatgt attgggtgt gcagtcaag 
      agagtggac gtaacgaaa caaagatga acgcaacgg 
      aaataaaaa caccgtcga aaatcatgg gaccgttac 
      tagtttaca aaaaccata acaaaaccg acaataaaa 
      atcctctcg aaccgttca cacctccga atttgcccc 
      aagacgaaa atccatact atcacaaaa acgaaaaag 
      acaagtttc tcggtcctt ataatccaa attataaac 
      aaaacaaga aacctggac ttggaaata accaagacg 
      agttgttac aattcattc accaacatc ccgaaatca 
      cgaattatc cttcgggat ccgttgtcg aaattcaca 
      aaccctcct tcaataatc gcccccaac atttcatcc 
      cctatgtca tcccacaga acggtcatt cggacgact 
      taccacctg gcataaccc tcgaacaaa ttttgcaag 
      aagtgcata aaaacgcaa acccgaatg gtcatattt 
      tacccttcc ctaatcatt tcactaacg gaccggtac 
      ttttagccc cacaaagtc caattttgc ctagacata 
      acgcaacgt ttccaggcc agcagtttc cagggggtt 
      tccccacga tgcaacagg atgatatta gtgtgacgg 
      cacttgaag ccgtggacg ctcacaccg tgcgtttgg 
      cttttgtgg tcccttcgg tcattgtgg gcttgcatg 
      gacgagtgg aagtgcgct acactcatc acgacgatt 
      cggacgtaa attcgtggc aattgcaag ttttcgcac 
      ctcccaatg acttgatcg ccactcctc aactcgaat 
      tcgaccttt tagacctca aacctgaca ccgaataca 
      agatcaaaa taacgataa cgttgcgcg acctccaca 
      acacgccaa gtagccgaa tatacaaaa taagaactc 
      tgaaggatt taaatgcaa caactttgc ctccgatga 
      tcccaatcg agcccgaat tggccatca gcgacataa 
      atgccactt gggtcacag tgatttggc gcaaattca 
      ccgaatacg aactgcaat tcatcgatg gagttcagt 
      tacgattca aggaaattg agggaaaaa tctacgacg 
      ctacgccac ttatccaca gatctgtga caagttaca 
      cactcgaga acctcaaac ctaacacga aatacgaaa 
      tcgggattt tcttcattc catttccgg gacagttga 
      cggaactac acgccgaac atatgctgc attttacca 
      cagccaacg aaatcgata cttacggct tcaatgtta 
      ctctggata tctctcata tcaagtcaa caagtgttg 
      agatttcct ggtccggag tgccgtacc cggaagaca 
      aatacgtca atatctacc gggcgattt accaaagcg 
      attccggga aggaggatc atagcacgt ttaaaatcg 
      ccaagcggg attccccca cgaaaacgg tcataagtg 
      acctaaaac cgggcacga gatatcgct tgtggttgg 
      aggtttacc tcaccaatg gtaaaatta agacgagta 
      atgttcaag actttataa cgaagccga gggcggcgc 
      cggctttag gggcggcca cccaatcgg ataaattat 
      cagcagggg agcaaaagg gcgactatt atgggcccc 
      tagtcattg ttgctatcc tagcggcga ttgccatta 
      tgagtacgt tgattttgt tgctgattt tagttagga 
      gacacaatc agaataaag ctgccatta cgccgcccc 
      cgccgaggg ttagtcaat cggcctacg ataacccaa 
      cgtacaaag tggagattc aacaagaga caatggggt 
      tatgaaagt gaaagacct cgactggtg taaatatta 
      ggcaataga tttacgtgt aagaaatcc tgattaaga 
      attttgtat ttaatttta gagttaggt tatgtaata 
      taatattcc gactattgt gtattattt atttaaatt 
      tgtaggata tttaaacca ttcaagatg ttactgttt 
      gcaattgat attttccta aaattaaat aaaagaact 
      aaaaacat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY