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Gene information
General information
TC010081 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9354362..9381342
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC010081
    iBeetle screen iB_04758

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010081 RA
      MFANTTISS RSSSSCSRD SSMPSLRMR NPLSPKTSY 
NPLFRHSDA FTSFDASHF IFQEQKRKD KKNPPILRR 
YHSHDSHHN FDDMRIDSK DDTPDSTKS RASTGSESG 
HEYEARDFL ERYSLPRVV RVSGGEPLL LYRCFDSFT 
KIEARGVFG KKGKEKTDG QILHFPEGY SGWFSLTNE 
KGEKSAVVY TSVLQLVRE QVCSFVSMQ PFTAYASQT 
ADLTDNSGK VQYLKTQVR AGQLFQLRA VFQHKERPD 
GGKNSTRSS RIPRSARDK DLANRYAQL VSQNNQELY 
VPLTAKGEF YEIHSSMKA KLCFPGMLD KTGPLALDK 
DCLYRITHL LRRVALPVK VKLITGPLP PGLPKDFTD 
TFILENKFQ EPLMVTCTL PPLNQDIIQ HQISCINLN 
SNVKLSKST LGFDSENRL FKSQRLQAA LAFCHKNVE 
SWYREVRLA PDLKQEELC PECRKTSSE CEGNCAAPD 
FDNRQIITK EVLEKFPKP KKWYKHFKL LGKNDEGPQ 
FADLRDPLD GMEKGKSIE RYKDMSKLI EEKFGRKSY 
NPVKKSASF MFSTKRIDL EETNHQVKN NPALLKCQS 
LDTQLYSIA ETQKINQDR GSLRSYDFQ MCILDETSS 
KDNLDEIKP ISEVKLKKT KPDLIPTLP KADPDHSYI 
TERLCSEFH VKTKVQKSK CSKMEVRLS GRHQKSALT 
VYEPQKTVI VQVHEVKSD GFVNVDDIP YSHVADEVC 
QRPSGGKST DENIYAEIC ESSTCTRCD NSKLCECKR 
TKKETEYCY VKLGSNGDS VTQSDSDEA IYNTLR
    CDS sequence
    • >TC010081 RA
      atgtttgcc aacacgacc atctcttca cggagcagt 
tccagctgc agtcgcgac tcctcaatg ccctccctt 
cgaatgcga aaccctctc tcacccaaa acctcctac 
aaccctctt ttccgccac tctgacgct ttcaccagc 
ttcgacgct tcccacttc atcttccaa gagcaaaaa 
cggaaagac aagaagaat cctcccatt ctgcgacgg 
taccactcc catgactct catcacaat ttcgacgac 
atgcgaata gactcaaaa gacgacacc ccggactcg 
accaaaagc agggctagt accggatcg gagtccggg 
cacgagtac gaagccagg gattttctg gagcggtac 
tctctacca agggttgtg cgagtaagt gggggcgag 
cccttgctg ctgtatcgg tgctttgac agttttacg 
aagattgaa gcgagaggg gtgtttggg aagaaggga 
aaggagaag actgatggg caaatttta cattttcca 
gaagggtac tcaggctgg ttctctttg acgaacgaa 
aaaggtgaa aagagcgcc gtggtgtac acgtcggtt 
ttgcaactc gtccgagaa caagtctgc tctttcgtc 
agcatgcaa ccgttcacg gcctatgcc tcccaaacg 
gcagacctt acagacaac agcggcaaa gtgcaatat 
ttaaagacc caagtccgc gcgggccag ctcttccaa 
ctccgggcg gtcttccag cataaggag cgccccgac 
ggcggcaaa aactcgaca aggtcctcc aggatacct 
cgctcggcc cgtgacaaa gaccttgca aaccgttac 
gctcaactc gtcagtcaa aacaaccaa gaattgtac 
gtcccactc acggcgaaa ggcgaattc tacgaaatc 
cacagttcg atgaaagcg aagctctgc ttccctggg 
atgttggat aaaactgga ccgctggct ctggacaaa 
gactgtctt tatcgaatt acgcactta ttgagacga 
gtggcactt ccagtcaaa gtcaaattg atcaccggc 
ccgctgcca cccgggctg cccaaagat ttcacagat 
actttcatc ctagagaac aaatttcaa gaacctcta 
atggtcact tgtactcta ccacctctg aatcaagac 
atcatccag caccaaatc agctgcatc aacctcaat 
tctaatgtg aaattatca aaaagtacc ttgggcttt 
gactccgaa aaccgcctc ttcaagtcg cagcgactc 
caagcagct ttggcgttt tgtcacaaa aacgtcgaa 
agttggtac cgggaagtc cgactagct cctgatttg 
aaacaggag gaactttgc cccgaatgc aggaaaacg 
tcaagtgaa tgtgagggc aactgtgcg gccccagac 
ttcgacaat cgacaaatc ataacgaaa gaagtcttg 
gaaaaattc ccaaaaccg aaaaaatgg tacaaacac 
tttaaactt ctgggcaaa aacgacgaa ggcccccag 
tttgccgac ctgagggac cccctcgac ggcatggaa 
aagggcaag agcatagag cgatataaa gacatgtcg 
aaactaatc gaagaaaaa ttcggccgg aaaagctac 
aacccggtg aaaaaatcc gccagtttt atgttttcc 
accaagcgt atcgacttg gaggaaacc aaccaccaa 
gttaaaaac aaccctgct ttgctcaaa tgtcagagt 
ttagacacc caactgtat tcaatcgct gaaacccag 
aaaataaac caagaccgc ggctctctc cgaagctac 
gacttccag atgtgcatt ttggacgaa accagcagc 
aaagacaac ctcgacgaa atcaagccc atctccgag 
gtgaagctg aagaagact aagccggac ctcatcccc 
acgctaccc aaagccgat cccgaccat tcctacatc 
accgagagg ctctgttcc gaattccat gtcaaaacc 
aaagtacaa aaaagcaag tgctccaag atggaagtg 
cgattgagt ggccgacac cagaagagc gcgctcacg 
gtgtatgaa ccccaaaaa acggtgata gtgcaggtg 
cacgaggtc aagtctgat ggctttgtt aacgttgat 
gatataccg tacagccat gtggctgat gaggtgtgt 
cagagaccc agtggcggg aaaagtacc gatgagaat 
atttatgcg gaaatctgc gagagttcg acgtgtacg 
agatgtgat aatagtaaa ttgtgcgag tgtaagagg 
actaagaag gagacggag tattgttat gtgaagttg 
ggctcaaat ggggacagt gtgacgcag tcagactcg 
gacgaagct atatacaat acgctaaga tga
    mRNA sequence
    • >TC010081 RA
      cggcgctgc tttttcgca ttcacatct cttgcagga 
accgcgtcg aagcagtcg cggagtcga cgaagagca 
gtgaactta gagattgta gccaataat gactttata 
tctgcaata attaaatag ctctgatga ctattgttg 
cgggttttg cgcttgatc gcacgttga agtctccag 
tgatcggtt gtgaaagtg cacattttg ggtgattct 
agtgaccta tcgtcgaat ttcttggac tgaagtacg 
atgtagaaa cctccagcg actccaaaa tgtttgcca 
acacgacca tctcttcac ggagcagtt ccagctgca 
gtcgcgact cctcaatgc cctcccttc gaatgcgaa 
accctctct cacccaaaa cctcctaca accctcttt 
tccgccact ctgacgctt tcaccagct tcgacgctt 
cccacttca tcttccaag agcaaaaac ggaaagaca 
agaagaatc ctcccattc tgcgacggt accactccc 
atgactctc atcacaatt tcgacgaca tgcgaatag 
actcaaaag acgacaccc cggactcga ccaaaagca 
gggctagta ccggatcgg agtccgggc acgagtacg 
aagccaggg attttctgg agcggtact ctctaccaa 
gggttgtgc gagtaagtg ggggcgagc ccttgctgc 
tgtatcggt gctttgaca gttttacga agattgaag 
cgagagggg tgtttggga agaagggaa aggagaaga 
ctgatgggc aaattttac attttccag aagggtact 
caggctggt tctctttga cgaacgaaa aaggtgaaa 
agagcgccg tggtgtaca cgtcggttt tgcaactcg 
tccgagaac aagtctgct ctttcgtca gcatgcaac 
cgttcacgg cctatgcct cccaaacgg cagacctta 
cagacaaca gcggcaaag tgcaatatt taaagaccc 
aagtccgcg cgggccagc tcttccaac tccgggcgg 
tcttccagc ataaggagc gccccgacg gcggcaaaa 
actcgacaa ggtcctcca ggatacctc gctcggccc 
gtgacaaag accttgcaa accgttacg ctcaactcg 
tcagtcaaa acaaccaag aattgtacg tcccactca 
cggcgaaag gcgaattct acgaaatcc acagttcga 
tgaaagcga agctctgct tccctggga tgttggata 
aaactggac cgctggctc tggacaaag actgtcttt 
atcgaatta cgcacttat tgagacgag tggcacttc 
cagtcaaag tcaaattga tcaccggcc cgctgccac 
ccgggctgc ccaaagatt tcacagata ctttcatcc 
tagagaaca aatttcaag aacctctaa tggtcactt 
gtactctac cacctctga atcaagaca tcatccagc 
accaaatca gctgcatca acctcaatt ctaatgtga 
aattatcaa aaagtacct tgggctttg actccgaaa 
accgcctct tcaagtcgc agcgactcc aagcagctt 
tggcgtttt gtcacaaaa acgtcgaaa gttggtacc 
gggaagtcc gactagctc ctgatttga aacaggagg 
aactttgcc ccgaatgca ggaaaacgt caagtgaat 
gtgagggca actgtgcgg ccccagact tcgacaatc 
gacaaatca taacgaaag aagtcttgg aaaaattcc 
caaaaccga aaaaatggt acaaacact ttaaacttc 
tgggcaaaa acgacgaag gcccccagt ttgccgacc 
tgagggacc ccctcgacg gcatggaaa agggcaaga 
gcatagagc gatataaag acatgtcga aactaatcg 
aagaaaaat tcggccgga aaagctaca acccggtga 
aaaaatccg ccagtttta tgttttcca ccaagcgta 
tcgacttgg aggaaacca accaccaag ttaaaaaca 
accctgctt tgctcaaat gtcagagtt tagacaccc 
aactgtatt caatcgctg aaacccaga aaataaacc 
aagaccgcg gctctctcc gaagctacg acttccaga 
tgtgcattt tggacgaaa ccagcagca aagacaacc 
tcgacgaaa tcaagccca tctccgagg tgaagctga 
agaagacta agccggacc tcatcccca cgctaccca 
aagccgatc ccgaccatt cctacatca ccgagaggc 
tctgttccg aattccatg tcaaaacca aagtacaaa 
aaagcaagt gctccaaga tggaagtgc gattgagtg 
gccgacacc agaagagcg cgctcacgg tgtatgaac 
cccaaaaaa cggtgatag tgcaggtgc acgaggtca 
agtctgatg gctttgtta acgttgatg atataccgt 
acagccatg tggctgatg aggtgtgtc agagaccca 
gtggcggga aaagtaccg atgagaata tttatgcgg 
aaatctgcg agagttcga cgtgtacga gatgtgata 
atagtaaat tgtgcgagt gtaagagga ctaagaagg 
agacggagt attgttatg tgaagttgg gctcaaatg 
gggacagtg tgacgcagt cagactcgg acgaagcta 
tatacaata cgctaagat gaagatgcg cttaaatct 
aatttaaat atttttatt aataataat attataagt 
aatattgtt ctcctacca ataaaatat ttaatacga 
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