| General information | |||
| TC010081 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 9354362..9381342 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC010081 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04758 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC010081 RA
MFANTTISS RSSSSCSRD SSMPSLRMR NPLSPKTSY NPLFRHSDA FTSFDASHF IFQEQKRKD KKNPPILRR YHSHDSHHN FDDMRIDSK DDTPDSTKS RASTGSESG HEYEARDFL ERYSLPRVV RVSGGEPLL LYRCFDSFT KIEARGVFG KKGKEKTDG QILHFPEGY SGWFSLTNE KGEKSAVVY TSVLQLVRE QVCSFVSMQ PFTAYASQT ADLTDNSGK VQYLKTQVR AGQLFQLRA VFQHKERPD GGKNSTRSS RIPRSARDK DLANRYAQL VSQNNQELY VPLTAKGEF YEIHSSMKA KLCFPGMLD KTGPLALDK DCLYRITHL LRRVALPVK VKLITGPLP PGLPKDFTD TFILENKFQ EPLMVTCTL PPLNQDIIQ HQISCINLN SNVKLSKST LGFDSENRL FKSQRLQAA LAFCHKNVE SWYREVRLA PDLKQEELC PECRKTSSE CEGNCAAPD FDNRQIITK EVLEKFPKP KKWYKHFKL LGKNDEGPQ FADLRDPLD GMEKGKSIE RYKDMSKLI EEKFGRKSY NPVKKSASF MFSTKRIDL EETNHQVKN NPALLKCQS LDTQLYSIA ETQKINQDR GSLRSYDFQ MCILDETSS KDNLDEIKP ISEVKLKKT KPDLIPTLP KADPDHSYI TERLCSEFH VKTKVQKSK CSKMEVRLS GRHQKSALT VYEPQKTVI VQVHEVKSD GFVNVDDIP YSHVADEVC QRPSGGKST DENIYAEIC ESSTCTRCD NSKLCECKR TKKETEYCY VKLGSNGDS VTQSDSDEA IYNTLR
- >TC010081 RA
atgtttgcc aacacgacc atctcttca cggagcagt tccagctgc agtcgcgac tcctcaatg ccctccctt cgaatgcga aaccctctc tcacccaaa acctcctac aaccctctt ttccgccac tctgacgct ttcaccagc ttcgacgct tcccacttc atcttccaa gagcaaaaa cggaaagac aagaagaat cctcccatt ctgcgacgg taccactcc catgactct catcacaat ttcgacgac atgcgaata gactcaaaa gacgacacc ccggactcg accaaaagc agggctagt accggatcg gagtccggg cacgagtac gaagccagg gattttctg gagcggtac tctctacca agggttgtg cgagtaagt gggggcgag cccttgctg ctgtatcgg tgctttgac agttttacg aagattgaa gcgagaggg gtgtttggg aagaaggga aaggagaag actgatggg caaatttta cattttcca gaagggtac tcaggctgg ttctctttg acgaacgaa aaaggtgaa aagagcgcc gtggtgtac acgtcggtt ttgcaactc gtccgagaa caagtctgc tctttcgtc agcatgcaa ccgttcacg gcctatgcc tcccaaacg gcagacctt acagacaac agcggcaaa gtgcaatat ttaaagacc caagtccgc gcgggccag ctcttccaa ctccgggcg gtcttccag cataaggag cgccccgac ggcggcaaa aactcgaca aggtcctcc aggatacct cgctcggcc cgtgacaaa gaccttgca aaccgttac gctcaactc gtcagtcaa aacaaccaa gaattgtac gtcccactc acggcgaaa ggcgaattc tacgaaatc cacagttcg atgaaagcg aagctctgc ttccctggg atgttggat aaaactgga ccgctggct ctggacaaa gactgtctt tatcgaatt acgcactta ttgagacga gtggcactt ccagtcaaa gtcaaattg atcaccggc ccgctgcca cccgggctg cccaaagat ttcacagat actttcatc ctagagaac aaatttcaa gaacctcta atggtcact tgtactcta ccacctctg aatcaagac atcatccag caccaaatc agctgcatc aacctcaat tctaatgtg aaattatca aaaagtacc ttgggcttt gactccgaa aaccgcctc ttcaagtcg cagcgactc caagcagct ttggcgttt tgtcacaaa aacgtcgaa agttggtac cgggaagtc cgactagct cctgatttg aaacaggag gaactttgc cccgaatgc aggaaaacg tcaagtgaa tgtgagggc aactgtgcg gccccagac ttcgacaat cgacaaatc ataacgaaa gaagtcttg gaaaaattc ccaaaaccg aaaaaatgg tacaaacac tttaaactt ctgggcaaa aacgacgaa ggcccccag tttgccgac ctgagggac cccctcgac ggcatggaa aagggcaag agcatagag cgatataaa gacatgtcg aaactaatc gaagaaaaa ttcggccgg aaaagctac aacccggtg aaaaaatcc gccagtttt atgttttcc accaagcgt atcgacttg gaggaaacc aaccaccaa gttaaaaac aaccctgct ttgctcaaa tgtcagagt ttagacacc caactgtat tcaatcgct gaaacccag aaaataaac caagaccgc ggctctctc cgaagctac gacttccag atgtgcatt ttggacgaa accagcagc aaagacaac ctcgacgaa atcaagccc atctccgag gtgaagctg aagaagact aagccggac ctcatcccc acgctaccc aaagccgat cccgaccat tcctacatc accgagagg ctctgttcc gaattccat gtcaaaacc aaagtacaa aaaagcaag tgctccaag atggaagtg cgattgagt ggccgacac cagaagagc gcgctcacg gtgtatgaa ccccaaaaa acggtgata gtgcaggtg cacgaggtc aagtctgat ggctttgtt aacgttgat gatataccg tacagccat gtggctgat gaggtgtgt cagagaccc agtggcggg aaaagtacc gatgagaat atttatgcg gaaatctgc gagagttcg acgtgtacg agatgtgat aatagtaaa ttgtgcgag tgtaagagg actaagaag gagacggag tattgttat gtgaagttg ggctcaaat ggggacagt gtgacgcag tcagactcg gacgaagct atatacaat acgctaaga tga
- >TC010081 RA
cggcgctgc tttttcgca ttcacatct cttgcagga accgcgtcg aagcagtcg cggagtcga cgaagagca gtgaactta gagattgta gccaataat gactttata tctgcaata attaaatag ctctgatga ctattgttg cgggttttg cgcttgatc gcacgttga agtctccag tgatcggtt gtgaaagtg cacattttg ggtgattct agtgaccta tcgtcgaat ttcttggac tgaagtacg atgtagaaa cctccagcg actccaaaa tgtttgcca acacgacca tctcttcac ggagcagtt ccagctgca gtcgcgact cctcaatgc cctcccttc gaatgcgaa accctctct cacccaaaa cctcctaca accctcttt tccgccact ctgacgctt tcaccagct tcgacgctt cccacttca tcttccaag agcaaaaac ggaaagaca agaagaatc ctcccattc tgcgacggt accactccc atgactctc atcacaatt tcgacgaca tgcgaatag actcaaaag acgacaccc cggactcga ccaaaagca gggctagta ccggatcgg agtccgggc acgagtacg aagccaggg attttctgg agcggtact ctctaccaa gggttgtgc gagtaagtg ggggcgagc ccttgctgc tgtatcggt gctttgaca gttttacga agattgaag cgagagggg tgtttggga agaagggaa aggagaaga ctgatgggc aaattttac attttccag aagggtact caggctggt tctctttga cgaacgaaa aaggtgaaa agagcgccg tggtgtaca cgtcggttt tgcaactcg tccgagaac aagtctgct ctttcgtca gcatgcaac cgttcacgg cctatgcct cccaaacgg cagacctta cagacaaca gcggcaaag tgcaatatt taaagaccc aagtccgcg cgggccagc tcttccaac tccgggcgg tcttccagc ataaggagc gccccgacg gcggcaaaa actcgacaa ggtcctcca ggatacctc gctcggccc gtgacaaag accttgcaa accgttacg ctcaactcg tcagtcaaa acaaccaag aattgtacg tcccactca cggcgaaag gcgaattct acgaaatcc acagttcga tgaaagcga agctctgct tccctggga tgttggata aaactggac cgctggctc tggacaaag actgtcttt atcgaatta cgcacttat tgagacgag tggcacttc cagtcaaag tcaaattga tcaccggcc cgctgccac ccgggctgc ccaaagatt tcacagata ctttcatcc tagagaaca aatttcaag aacctctaa tggtcactt gtactctac cacctctga atcaagaca tcatccagc accaaatca gctgcatca acctcaatt ctaatgtga aattatcaa aaagtacct tgggctttg actccgaaa accgcctct tcaagtcgc agcgactcc aagcagctt tggcgtttt gtcacaaaa acgtcgaaa gttggtacc gggaagtcc gactagctc ctgatttga aacaggagg aactttgcc ccgaatgca ggaaaacgt caagtgaat gtgagggca actgtgcgg ccccagact tcgacaatc gacaaatca taacgaaag aagtcttgg aaaaattcc caaaaccga aaaaatggt acaaacact ttaaacttc tgggcaaaa acgacgaag gcccccagt ttgccgacc tgagggacc ccctcgacg gcatggaaa agggcaaga gcatagagc gatataaag acatgtcga aactaatcg aagaaaaat tcggccgga aaagctaca acccggtga aaaaatccg ccagtttta tgttttcca ccaagcgta tcgacttgg aggaaacca accaccaag ttaaaaaca accctgctt tgctcaaat gtcagagtt tagacaccc aactgtatt caatcgctg aaacccaga aaataaacc aagaccgcg gctctctcc gaagctacg acttccaga tgtgcattt tggacgaaa ccagcagca aagacaacc tcgacgaaa tcaagccca tctccgagg tgaagctga agaagacta agccggacc tcatcccca cgctaccca aagccgatc ccgaccatt cctacatca ccgagaggc tctgttccg aattccatg tcaaaacca aagtacaaa aaagcaagt gctccaaga tggaagtgc gattgagtg gccgacacc agaagagcg cgctcacgg tgtatgaac cccaaaaaa cggtgatag tgcaggtgc acgaggtca agtctgatg gctttgtta acgttgatg atataccgt acagccatg tggctgatg aggtgtgtc agagaccca gtggcggga aaagtaccg atgagaata tttatgcgg aaatctgcg agagttcga cgtgtacga gatgtgata atagtaaat tgtgcgagt gtaagagga ctaagaagg agacggagt attgttatg tgaagttgg gctcaaatg gggacagtg tgacgcagt cagactcgg acgaagcta tatacaata cgctaagat gaagatgcg cttaaatct aatttaaat atttttatt aataataat attataagt aatattgtt ctcctacca ataaaatat ttaatacga t
