Status
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Gene information
General information
TC010081 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9354362..9381342
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010081
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04758

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010081 RA
      MFANTTISS RSSSSCSRD SSMPSLRMR NPLSPKTSY 
      NPLFRHSDA FTSFDASHF IFQEQKRKD KKNPPILRR 
      YHSHDSHHN FDDMRIDSK DDTPDSTKS RASTGSESG 
      HEYEARDFL ERYSLPRVV RVSGGEPLL LYRCFDSFT 
      KIEARGVFG KKGKEKTDG QILHFPEGY SGWFSLTNE 
      KGEKSAVVY TSVLQLVRE QVCSFVSMQ PFTAYASQT 
      ADLTDNSGK VQYLKTQVR AGQLFQLRA VFQHKERPD 
      GGKNSTRSS RIPRSARDK DLANRYAQL VSQNNQELY 
      VPLTAKGEF YEIHSSMKA KLCFPGMLD KTGPLALDK 
      DCLYRITHL LRRVALPVK VKLITGPLP PGLPKDFTD 
      TFILENKFQ EPLMVTCTL PPLNQDIIQ HQISCINLN 
      SNVKLSKST LGFDSENRL FKSQRLQAA LAFCHKNVE 
      SWYREVRLA PDLKQEELC PECRKTSSE CEGNCAAPD 
      FDNRQIITK EVLEKFPKP KKWYKHFKL LGKNDEGPQ 
      FADLRDPLD GMEKGKSIE RYKDMSKLI EEKFGRKSY 
      NPVKKSASF MFSTKRIDL EETNHQVKN NPALLKCQS 
      LDTQLYSIA ETQKINQDR GSLRSYDFQ MCILDETSS 
      KDNLDEIKP ISEVKLKKT KPDLIPTLP KADPDHSYI 
      TERLCSEFH VKTKVQKSK CSKMEVRLS GRHQKSALT 
      VYEPQKTVI VQVHEVKSD GFVNVDDIP YSHVADEVC 
      QRPSGGKST DENIYAEIC ESSTCTRCD NSKLCECKR 
      TKKETEYCY VKLGSNGDS VTQSDSDEA IYNTLR
    CDS sequence
    • >TC010081 RA
      atgtttgcc aacacgacc atctcttca cggagcagt 
      tccagctgc agtcgcgac tcctcaatg ccctccctt 
      cgaatgcga aaccctctc tcacccaaa acctcctac 
      aaccctctt ttccgccac tctgacgct ttcaccagc 
      ttcgacgct tcccacttc atcttccaa gagcaaaaa 
      cggaaagac aagaagaat cctcccatt ctgcgacgg 
      taccactcc catgactct catcacaat ttcgacgac 
      atgcgaata gactcaaaa gacgacacc ccggactcg 
      accaaaagc agggctagt accggatcg gagtccggg 
      cacgagtac gaagccagg gattttctg gagcggtac 
      tctctacca agggttgtg cgagtaagt gggggcgag 
      cccttgctg ctgtatcgg tgctttgac agttttacg 
      aagattgaa gcgagaggg gtgtttggg aagaaggga 
      aaggagaag actgatggg caaatttta cattttcca 
      gaagggtac tcaggctgg ttctctttg acgaacgaa 
      aaaggtgaa aagagcgcc gtggtgtac acgtcggtt 
      ttgcaactc gtccgagaa caagtctgc tctttcgtc 
      agcatgcaa ccgttcacg gcctatgcc tcccaaacg 
      gcagacctt acagacaac agcggcaaa gtgcaatat 
      ttaaagacc caagtccgc gcgggccag ctcttccaa 
      ctccgggcg gtcttccag cataaggag cgccccgac 
      ggcggcaaa aactcgaca aggtcctcc aggatacct 
      cgctcggcc cgtgacaaa gaccttgca aaccgttac 
      gctcaactc gtcagtcaa aacaaccaa gaattgtac 
      gtcccactc acggcgaaa ggcgaattc tacgaaatc 
      cacagttcg atgaaagcg aagctctgc ttccctggg 
      atgttggat aaaactgga ccgctggct ctggacaaa 
      gactgtctt tatcgaatt acgcactta ttgagacga 
      gtggcactt ccagtcaaa gtcaaattg atcaccggc 
      ccgctgcca cccgggctg cccaaagat ttcacagat 
      actttcatc ctagagaac aaatttcaa gaacctcta 
      atggtcact tgtactcta ccacctctg aatcaagac 
      atcatccag caccaaatc agctgcatc aacctcaat 
      tctaatgtg aaattatca aaaagtacc ttgggcttt 
      gactccgaa aaccgcctc ttcaagtcg cagcgactc 
      caagcagct ttggcgttt tgtcacaaa aacgtcgaa 
      agttggtac cgggaagtc cgactagct cctgatttg 
      aaacaggag gaactttgc cccgaatgc aggaaaacg 
      tcaagtgaa tgtgagggc aactgtgcg gccccagac 
      ttcgacaat cgacaaatc ataacgaaa gaagtcttg 
      gaaaaattc ccaaaaccg aaaaaatgg tacaaacac 
      tttaaactt ctgggcaaa aacgacgaa ggcccccag 
      tttgccgac ctgagggac cccctcgac ggcatggaa 
      aagggcaag agcatagag cgatataaa gacatgtcg 
      aaactaatc gaagaaaaa ttcggccgg aaaagctac 
      aacccggtg aaaaaatcc gccagtttt atgttttcc 
      accaagcgt atcgacttg gaggaaacc aaccaccaa 
      gttaaaaac aaccctgct ttgctcaaa tgtcagagt 
      ttagacacc caactgtat tcaatcgct gaaacccag 
      aaaataaac caagaccgc ggctctctc cgaagctac 
      gacttccag atgtgcatt ttggacgaa accagcagc 
      aaagacaac ctcgacgaa atcaagccc atctccgag 
      gtgaagctg aagaagact aagccggac ctcatcccc 
      acgctaccc aaagccgat cccgaccat tcctacatc 
      accgagagg ctctgttcc gaattccat gtcaaaacc 
      aaagtacaa aaaagcaag tgctccaag atggaagtg 
      cgattgagt ggccgacac cagaagagc gcgctcacg 
      gtgtatgaa ccccaaaaa acggtgata gtgcaggtg 
      cacgaggtc aagtctgat ggctttgtt aacgttgat 
      gatataccg tacagccat gtggctgat gaggtgtgt 
      cagagaccc agtggcggg aaaagtacc gatgagaat 
      atttatgcg gaaatctgc gagagttcg acgtgtacg 
      agatgtgat aatagtaaa ttgtgcgag tgtaagagg 
      actaagaag gagacggag tattgttat gtgaagttg 
      ggctcaaat ggggacagt gtgacgcag tcagactcg 
      gacgaagct atatacaat acgctaaga tga
    mRNA sequence
    • >TC010081 RA
      cggcgctgc tttttcgca ttcacatct cttgcagga 
      accgcgtcg aagcagtcg cggagtcga cgaagagca 
      gtgaactta gagattgta gccaataat gactttata 
      tctgcaata attaaatag ctctgatga ctattgttg 
      cgggttttg cgcttgatc gcacgttga agtctccag 
      tgatcggtt gtgaaagtg cacattttg ggtgattct 
      agtgaccta tcgtcgaat ttcttggac tgaagtacg 
      atgtagaaa cctccagcg actccaaaa tgtttgcca 
      acacgacca tctcttcac ggagcagtt ccagctgca 
      gtcgcgact cctcaatgc cctcccttc gaatgcgaa 
      accctctct cacccaaaa cctcctaca accctcttt 
      tccgccact ctgacgctt tcaccagct tcgacgctt 
      cccacttca tcttccaag agcaaaaac ggaaagaca 
      agaagaatc ctcccattc tgcgacggt accactccc 
      atgactctc atcacaatt tcgacgaca tgcgaatag 
      actcaaaag acgacaccc cggactcga ccaaaagca 
      gggctagta ccggatcgg agtccgggc acgagtacg 
      aagccaggg attttctgg agcggtact ctctaccaa 
      gggttgtgc gagtaagtg ggggcgagc ccttgctgc 
      tgtatcggt gctttgaca gttttacga agattgaag 
      cgagagggg tgtttggga agaagggaa aggagaaga 
      ctgatgggc aaattttac attttccag aagggtact 
      caggctggt tctctttga cgaacgaaa aaggtgaaa 
      agagcgccg tggtgtaca cgtcggttt tgcaactcg 
      tccgagaac aagtctgct ctttcgtca gcatgcaac 
      cgttcacgg cctatgcct cccaaacgg cagacctta 
      cagacaaca gcggcaaag tgcaatatt taaagaccc 
      aagtccgcg cgggccagc tcttccaac tccgggcgg 
      tcttccagc ataaggagc gccccgacg gcggcaaaa 
      actcgacaa ggtcctcca ggatacctc gctcggccc 
      gtgacaaag accttgcaa accgttacg ctcaactcg 
      tcagtcaaa acaaccaag aattgtacg tcccactca 
      cggcgaaag gcgaattct acgaaatcc acagttcga 
      tgaaagcga agctctgct tccctggga tgttggata 
      aaactggac cgctggctc tggacaaag actgtcttt 
      atcgaatta cgcacttat tgagacgag tggcacttc 
      cagtcaaag tcaaattga tcaccggcc cgctgccac 
      ccgggctgc ccaaagatt tcacagata ctttcatcc 
      tagagaaca aatttcaag aacctctaa tggtcactt 
      gtactctac cacctctga atcaagaca tcatccagc 
      accaaatca gctgcatca acctcaatt ctaatgtga 
      aattatcaa aaagtacct tgggctttg actccgaaa 
      accgcctct tcaagtcgc agcgactcc aagcagctt 
      tggcgtttt gtcacaaaa acgtcgaaa gttggtacc 
      gggaagtcc gactagctc ctgatttga aacaggagg 
      aactttgcc ccgaatgca ggaaaacgt caagtgaat 
      gtgagggca actgtgcgg ccccagact tcgacaatc 
      gacaaatca taacgaaag aagtcttgg aaaaattcc 
      caaaaccga aaaaatggt acaaacact ttaaacttc 
      tgggcaaaa acgacgaag gcccccagt ttgccgacc 
      tgagggacc ccctcgacg gcatggaaa agggcaaga 
      gcatagagc gatataaag acatgtcga aactaatcg 
      aagaaaaat tcggccgga aaagctaca acccggtga 
      aaaaatccg ccagtttta tgttttcca ccaagcgta 
      tcgacttgg aggaaacca accaccaag ttaaaaaca 
      accctgctt tgctcaaat gtcagagtt tagacaccc 
      aactgtatt caatcgctg aaacccaga aaataaacc 
      aagaccgcg gctctctcc gaagctacg acttccaga 
      tgtgcattt tggacgaaa ccagcagca aagacaacc 
      tcgacgaaa tcaagccca tctccgagg tgaagctga 
      agaagacta agccggacc tcatcccca cgctaccca 
      aagccgatc ccgaccatt cctacatca ccgagaggc 
      tctgttccg aattccatg tcaaaacca aagtacaaa 
      aaagcaagt gctccaaga tggaagtgc gattgagtg 
      gccgacacc agaagagcg cgctcacgg tgtatgaac 
      cccaaaaaa cggtgatag tgcaggtgc acgaggtca 
      agtctgatg gctttgtta acgttgatg atataccgt 
      acagccatg tggctgatg aggtgtgtc agagaccca 
      gtggcggga aaagtaccg atgagaata tttatgcgg 
      aaatctgcg agagttcga cgtgtacga gatgtgata 
      atagtaaat tgtgcgagt gtaagagga ctaagaagg 
      agacggagt attgttatg tgaagttgg gctcaaatg 
      gggacagtg tgacgcagt cagactcgg acgaagcta 
      tatacaata cgctaagat gaagatgcg cttaaatct 
      aatttaaat atttttatt aataataat attataagt 
      aatattgtt ctcctacca ataaaatat ttaatacga 
      t
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