Status
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Gene information
General information
TC010074 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9530915..9550165
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010074
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04756

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010074 RA
      MADHGDFDD EELVSSNTN QRNWRGILI ALLVIVVVL 
      ALIVTSVVL LTPPDEGPR IKGARFKLQ DILSHDFQP 
      LRFNGTWIS GEELVYKDQ WGGISIMHA SNLSVRTLM 
      SNQTFLRLN PARFQLSAD QRYLLLAQN VQKLFRHSY 
      LAQYTIYDI QTGNDFPLR PMPETEEHP YLLLAEWAP 
      RGRGLIMVQ DYDIYYRKS PTSHTGYRI TNSAVPGVV 
      SHGVPDWLY EEEILGSNS ALWMSSDGL LLVFASFND 
      SLVEELRFP WYGSANEGR LYPDIRSLR YPKPGTRNP 
      KVTLTIADL ADTSNIKIK TVIPPVALA NVEYYFTSV 
      SWISLTEIS VVWLNRPQN LSLITICTS PKWDCRETQ 
      RITSDGHGW VDMGDSPVF GTDSSNYIT VAPVRDGPA 
      GYFRHAVSV NIPKRRIIP LTHGKYEVT RILTWDHSN 
      DVIYFLGIP ESKPGQQHL YRVTSVPPR TGATLPPPV 
      CLTCVPEPA PTTPPYFFM DEDIYLLKT KYWDEDEES 
      DVITTPAPK RRKKKPKVT PTEPSCLYH NAIFSPNSK 
      YYVLECLGP GIPYSVLYK TAMPKPKLI VNLQNNTKL 
      REMVAKMAM PQIKTFPVQ ISGGYQAHV RLHLPPGLR 
      EDEITRYPL VVQVYGGPG SQLVTERWR VDWNTYLTG 
      NKDFIIAQI DGRGSGGQG HQLLHKVYY KLGSVEVAD 
      QLEVTEYLR DTLHFIDKR RVAVWGWSY GGFVAALAL 
      ASPKSVFRC AIAVAPVTN WKLYDSAYT ERYMGLPNV 
      TDNYKGYDE ADVSKKAHL LKDKMFYLV HGSADDNVH 
      LQQSMALVK SLSEAGTLF RQQIYPDES HGLGGVKKH 
      LYKSMGQFF DDCFQKQVP PEFKAGLRN DVE
    CDS sequence
    • >TC010074 RA
      atggcagat cacggtgac ttcgacgac gaggagctc 
      gtgtccagc aacacgaac caaaggaat tggcgcgga 
      atcctcata gcgctcctc gtcatcgtg gtagttctg 
      gccttgatt gtgacatct gtagtcctc ctaactccc 
      ccagacgaa ggtccccga attaaagga gccagattt 
      aagctacag gacatcctc agccacgac ttccagccg 
      ttgaggttt aacggcacg tggatttca ggcgaagaa 
      ctagtctac aaggaccaa tggggcggc atcagcatt 
      atgcacgcg tccaacctg tctgttcgc actttgatg 
      tccaaccaa accttcttg cggttgaat ccagccaga 
      tttcagctc agtgcggac cagcgctat ctgcttctg 
      gcacagaac gtgcagaag ttgttcagg cactcctac 
      ttggcgcag tacacaatc tacgacatt cagacaggt 
      aacgatttc cctttgcgt ccgatgccg gaaacggaa 
      gagcacccc tacctgctc ttagccgaa tgggcgccc 
      cgaggccgc ggcctcata atggtgcag gattacgat 
      atttactac aggaagagt cccacttcg cacactggc 
      tataggata accaacagc gcggttccg ggcgtggtg 
      agtcacgga gttccggac tggctctac gaagaagag 
      atcctgggc agcaacagc gctttgtgg atgtctagc 
      gacggcctt ttgttagtt ttcgcgtct ttcaacgac 
      agtttggtg gaggagttg agattcccg tggtatgga 
      tcggcgaac gaaggccgg ctttatccc gatatccgg 
      tcgctccgg tatcccaaa cctggaacc agaaacccc 
      aaggtaacc ctgaccata gccgactta gccgacacg 
      tcgaatatc aagataaaa accgtgatc ccaccagtt 
      gcactggcc aatgtggaa tattacttc acttcagtt 
      tcttggatc agtttgacg gagatttca gtggtttgg 
      ttaaacagg ccgcagaac ttatccctg atcacaata 
      tgcaccagt cctaaatgg gattgccga gagacccaa 
      aggatcacc agcgacggt cacggctgg gtggacatg 
      ggcgacagc cccgtcttc ggcaccgac agcagcaat 
      tacatcacg gtggcaccc gtcagagac ggacccgcc 
      ggttatttc cgtcacgcc gtctccgtc aatatcccg 
      aagaggcga attattcct ctcacacat gggaagtac 
      gaggtgaca cgtattctt acttgggac cattcgaac 
      gatgtcatt tacttcttg ggaataccc gagagtaag 
      ccgggccag cagcacctc taccgagtg acttcagtc 
      ccgccacgg accggagcg acgctgcct cctccggtg 
      tgtctaacc tgcgtcccg gagcctgcc cctaccact 
      cctccttac ttcttcatg gacgaagat atctacctc 
      ctcaaaacc aaatactgg gatgaagac gaggaaagc 
      gatgtgatc acaacaccg gcgcccaag cggcgcaaa 
      aagaaacca aaagtaaca ccaactgaa ccttcatgc 
      ctctaccac aacgctata tttagccct aactcgaaa 
      tactacgtc ttggagtgc cttggtccg gggattccc 
      tacagtgtt ttgtacaag acggcgatg cccaagccg 
      aaacttatc gtcaatctg cagaacaat accaagttg 
      agggagatg gtggccaag atggccatg ccacaaatc 
      aagaccttc ccggtccag atttccggg gggtaccaa 
      gcgcatgta aggctgcac ttaccgccc ggcttgcgg 
      gaagatgag attaccagg tatccgctc gtcgtccaa 
      gtgtacggc ggtcctggt agtcagctg gtgacggaa 
      aggtggcga gtcgattgg aacacgtat ttgacagga 
      aacaaggac tttattatt gcacagatc gacggacga 
      gggtctgga ggtcaaggc caccaactt ctgcacaaa 
      gtttattac aaattgggg tcggtcgaa gtggctgat 
      cagctagaa gttacagaa tacttgcga gacacactc 
      cacttcatc gataaacgg agggtggcc gtttgggga 
      tggagttat ggtgggttc gtcgccgca ttagcgctg 
      gcaagtccc aaaagtgtt tttcgatgt gccatcgcc 
      gtggctccg gttaccaat tggaagctc tacgactcc 
      gcctatacg gagcgctac atgggcctg cctaacgtg 
      acggacaac tacaaagga tacgacgag gccgacgtt 
      agcaaaaaa gcgcatctg ctcaaggat aaaatgttt 
      tatctagtg cacggctcg gccgacgac aatgtccac 
      cttcagcaa tctatggct ttggtgaaa tccctctca 
      gaggctgga acgcttttc cgacagcag atttacccg 
      gacgaaagc cacgggttg ggcggagtg aaaaaacac 
      ttgtacaag agcatgggc caatttttc gacgactgc 
      ttccagaaa caagttcct ccagaattc aaagcggga 
      ttaaggaat gatgtcgag tag
    mRNA sequence
    • >TC010074 RA
      tcgttgata aagaaagac gcgtcgcga cgcctgctt 
      caaaactcc acttcagtg tgaccgaag aactgcgtt 
      cgagtcgtg catgaaatg agccccagc tcggcgaag 
      acggcgccg acttgctcc tgagttatg ctagtagtg 
      taggagtgc tcctcgtgg gtgtgcaca agacatggc 
      agatcacgg tgacttcga cgacgagga gctcgtgtc 
      cagcaacac gaaccaaag gaattggcg cggaatcct 
      catagcgct cctcgtcat cgtggtagt tctggcctt 
      gattgtgac atctgtagt cctcctaac tcccccaga 
      cgaaggtcc ccgaattaa aggagccag atttaagct 
      acaggacat cctcagcca cgacttcca gccgttgag 
      gtttaacgg cacgtggat ttcaggcga agaactagt 
      ctacaagga ccaatgggg cggcatcag cattatgca 
      cgcgtccaa cctgtctgt tcgcacttt gatgtccaa 
      ccaaacctt cttgcggtt gaatccagc cagatttca 
      gctcagtgc ggaccagcg ctatctgct tctggcaca 
      gaacgtgca gaagttgtt caggcactc ctacttggc 
      gcagtacac aatctacga cattcagac aggtaacga 
      tttcccttt gcgtccgat gccggaaac ggaagagca 
      cccctacct gctcttagc cgaatgggc gccccgagg 
      ccgcggcct cataatggt gcaggatta cgatattta 
      ctacaggaa gagtcccac ttcgcacac tggctatag 
      gataaccaa cagcgcggt tccgggcgt ggtgagtca 
      cggagttcc ggactggct ctacgaaga agagatcct 
      gggcagcaa cagcgcttt gtggatgtc tagcgacgg 
      ccttttgtt agttttcgc gtctttcaa cgacagttt 
      ggtggagga gttgagatt cccgtggta tggatcggc 
      gaacgaagg ccggcttta tcccgatat ccggtcgct 
      ccggtatcc caaacctgg aaccagaaa ccccaaggt 
      aaccctgac catagccga cttagccga cacgtcgaa 
      tatcaagat aaaaaccgt gatcccacc agttgcact 
      ggccaatgt ggaatatta cttcacttc agtttcttg 
      gatcagttt gacggagat ttcagtggt ttggttaaa 
      caggccgca gaacttatc cctgatcac aatatgcac 
      cagtcctaa atgggattg ccgagagac ccaaaggat 
      caccagcga cggtcacgg ctgggtgga catgggcga 
      cagccccgt cttcggcac cgacagcag caattacat 
      cacggtggc acccgtcag agacggacc cgccggtta 
      tttccgtca cgccgtctc cgtcaatat cccgaagag 
      gcgaattat tcctctcac acatgggaa gtacgaggt 
      gacacgtat tcttacttg ggaccattc gaacgatgt 
      catttactt cttgggaat acccgagag taagccggg 
      ccagcagca cctctaccg agtgacttc agtcccgcc 
      acggaccgg agcgacgct gcctcctcc ggtgtgtct 
      aacctgcgt cccggagcc tgcccctac cactcctcc 
      ttacttctt catggacga agatatcta cctcctcaa 
      aaccaaata ctgggatga agacgagga aagcgatgt 
      gatcacaac accggcgcc caagcggcg caaaaagaa 
      accaaaagt aacaccaac tgaaccttc atgcctcta 
      ccacaacgc tatatttag ccctaactc gaaatacta 
      cgtcttgga gtgccttgg tccggggat tccctacag 
      tgttttgta caagacggc gatgcccaa gccgaaact 
      tatcgtcaa tctgcagaa caataccaa gttgaggga 
      gatggtggc caagatggc catgccaca aatcaagac 
      cttcccggt ccagatttc cggggggta ccaagcgca 
      tgtaaggct gcacttacc gcccggctt gcgggaaga 
      tgagattac caggtatcc gctcgtcgt ccaagtgta 
      cggcggtcc tggtagtca gctggtgac ggaaaggtg 
      gcgagtcga ttggaacac gtatttgac aggaaacaa 
      ggactttat tattgcaca gatcgacgg acgagggtc 
      tggaggtca aggccacca acttctgca caaagttta 
      ttacaaatt ggggtcggt cgaagtggc tgatcagct 
      agaagttac agaatactt gcgagacac actccactt 
      catcgataa acggagggt ggccgtttg gggatggag 
      ttatggtgg gttcgtcgc cgcattagc gctggcaag 
      tcccaaaag tgtttttcg atgtgccat cgccgtggc 
      tccggttac caattggaa gctctacga ctccgccta 
      tacggagcg ctacatggg cctgcctaa cgtgacgga 
      caactacaa aggatacga cgaggccga cgttagcaa 
      aaaagcgca tctgctcaa ggataaaat gttttatct 
      agtgcacgg ctcggccga cgacaatgt ccaccttca 
      gcaatctat ggctttggt gaaatccct ctcagaggc 
      tggaacgct tttccgaca gcagattta cccggacga 
      aagccacgg gttgggcgg agtgaaaaa acacttgta 
      caagagcat gggccaatt tttcgacga ctgcttcca 
      gaaacaagt tcctccaga attcaaagc gggattaag 
      gaatgatgt cgagtagta cacttttaa cgatttcct 
      caactaagt gatgccaaa ctgaggatg tccctatcg 
      ctttatata caacatcca atttgattc cgacgggat 
      gttagtgat gaaagtgcc aaattttga tgttctcga 
      tgttagtag acttgcaat tgcaataag agtggaact 
      caggaaatc agaaggaac gcccttaaa cccctagaa 
      aacagaaca ataagggac accttgcgc tgttctgcg 
      tagctacaa tcagtatta attggcgaa gaacatgac 
      atatcttaa gtcgactcg tcttcaagg ttccacgtc 
      cctgcattt taatgaata tagtcaatg ataacttgt 
      ggatagtag attttaaaa tgaccaaat cctagatag 
      acaacctcc aataatcat tcaagtttc catgatatt 
      ttagctggt aaggatagt gttcagagg gtctatttc 
      gagccataa gccttaact ctgaccttc tcgtcattt 
      tagttcccg ttcttttat cgttttgta caaaaataa 
      gtcaatttt taacgatgg cgtactcaa ttctgaaca 
      ctggtgact tttatcatg tactcatct tgtaaatat 
      ttgattcgt aggtagctg tacagatat gggtccaat 
      aactgttct atttttcca aaagtgcac ggtgtgact 
      tacgcacga tctagcaat aaccatttc gctctaata 
      actcttgaa caatctgct agtcaaaat agaccattg 
      tacatatgg cttgaccca aacaatctg atagtctcg 
      atagatact aaaatagtg agattagta ggtttcagt 
      tggtagaaa tttgttatt gtacagcga ggaaaactt 
      taccattgt tgccaactt tgaaagctc aaaaaatgt 
      gagacgctg ttcaatatt gtttgtata taaaagaca 
      agttttttg aatgagttc ttttagaag agattgttt 
      tgttgttaa cacgcctta atttcgtcg atcatgtga 
      tacctccta tcatccgtt ttgaggaaa tttattgta 
      aatatctta aagacgttc ttgcatcta tcattcggt 
      ggttgttca cacaatagt atacttgaa gtagattat 
      tgaaaattt actagatta ttatattga aaattataa 
      aacaaataa agacaaatt caaact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY