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Gene information
General information
TC033829
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010071,TC010072 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9553585..9557185
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC033829
      iBeetle screen iB_04755

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
      Days post injection

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC033829 RA
        MKVSARVKD EWLQIPCKD GTNTVRWLG EEAWRKYQK 
LMQGVKIEE HDIINVIQE VRKTRGGAL LDPDDIISE 
VLDNDDFVN IVLTTDTQV NVGVIPEEL KHVLEPIPS 
NFLPADNYV FVDGSNLTT EMLVNLGNG EFKIKLSKE 
AINSVLKSR SLVESLLGE ERSVYGITT GIGSFSEVS 
IPYDKLEDL QYNIIRSHA AGVGNPLSP ARTRMLLAL 
RINVLAKGY SGISLNTLQ GLVDAFNAS CLSWVPEQG 
SVGASGDLA PLAHLALGI VGEGKMWSP KTGWGEAKR 
VLKLNGLKP LVLGPKEAD ALVNGTQLI TSIGAEAVE 
RALHIAKQA DVVAALTLE VLKGTSRAF DSDVQKIRP 
HKGQIAVAK RLRALLHSD TYPSEIAES HRFCNKVQD 
AYTLRCCPQ VHGIVHDTI DFVQGIIQT EMNSGTDNP 
LVFADRGEI ISAGNFHGE YPAKVLDFL SIAVHELAS 
MSEKRIERL VNPALSSLP AYLVKDGGL NSGFMVAHC 
TAAALVSEN KVVCHPSSV DSMSTSAGQ EDHVSMGCF 
AARKALQVI ENVENVIAI ELLAACQAI EFLRPLRTT 
TPLEEVYAT VRSVVQPWD KDRFMAPDI EAVKKLLVE 
NKIWNTVQH YMMFYHAPQ EQETRVFSP TASSLGPER 
AKPRSSGRI SLKRKSFAQ EAEF
      CDS sequence
      • >TC033829 RA
        atgaaggtt tctgcccga gttaaagac gagtggtta 
caaattcct tgcaaagat ggcacaaac acggttaga 
tggctagga gaagaagca tggaggaag tatcagaaa 
ttgatgcaa ggggtcaaa atcgaagaa cacgatata 
atcaatgtc atacaagaa gtgaggaaa acgcgagga 
ggtgcttta ttggatccc gacgacatc atctctgag 
gttttagat aatgacgat tttgtcaac attgttcta 
actacagac acgcaagtc aatgttggt gttatccca 
gaggagctt aaacatgtt ttggagcca attccaagc 
aattttctc ccggcggat aattacgtt tttgtggac 
ggttctaac ttaactacg gaaatgctg gtgaattta 
ggaaatgga gaatttaag attaagtta tcaaaagaa 
gccatcaat agcgtcctc aaatctcgt tctttagtc 
gaatccctt cttggggaa gagcgctcg gtttacggc 
attacgaca ggcatcgga agcttctca gaagtcagc 
attccgtat gataagctc gaagacctg caatacaac 
ataatcaga tcgcacgcg gctggcgtg ggtaatcct 
ttaagcccc gcaagaact agaatgttg ctcgcatta 
aggattaac gttctagca aagggttac agcggcata 
tccttaaac actctacag ggcctcgtc gacgcattt 
aatgcttcg tgcttatcg tgggtaccg gaacaggga 
tcagtgggc gccagcggc gacttggcc cctttagcc 
cacttggcg ctcgggatt gtgggcgag ggcaagatg 
tggagcccc aaaaccggc tggggagaa gccaaacgg 
gttttgaaa ctaaatggg ctgaagcct ctggttctg 
ggccccaaa gaggcggat gcactggtc aatgggacc 
caacttatc actagcatt ggggccgaa gctgtggaa 
agggctctg catatagca aaacaggcc gatgttgtt 
gctgcgctt actttagag gtgttgaaa gggacgtct 
cgtgcgttt gattcagac gtccagaag ataaggccc 
cacaaagga cagatcgct gtagccaaa cgattgaga 
gcgctgctt cactctgat acttatccg tccgaaatt 
gcagaaagc cacaggttt tgtaataag gtccaagac 
gcatacact ttgagatgt tgtccccag gttcacgga 
attgttcac gacacaatc gattttgta caaggaatc 
atccagact gagatgaac agcgggact gataatcct 
cttgttttt gctgatcga ggcgaaatc atttcagcg 
ggaaatttc cacggggaa taccctgcc aaagtttta 
gacttccta agtatagcg gttcacgag ttggcttca 
atgagcgaa aaacgaatc gagcgatta gtaaacccc 
gcattaagc agccttccg gcttatctc gtgaaagat 
ggaggcctc aattccggt ttcatggtg gcgcattgc 
acggctgca gctttagtt tctgaaaac aaagttgtt 
tgccaccct tcgtcagta gacagtatg agcacgagc 
gccggtcag gaggatcac gtttcgatg ggatgtttc 
gctgctcga aaagctctc caagtgatc gaaaatgtg 
gaaaatgtg attgctatc gagctcctt gcggcatgt 
caagcaatt gagtttttg aggcctttg aggacgacg 
acgcctttg gaggaggtt tatgctaca gtgaggagt 
gtggtccag ccttgggac aaggacagg tttatggcg 
cccgatatt gaagccgtt aagaagctc ttagttgag 
aataaaatt tggaatact gtgcaacat tatatgatg 
ttttatcat gcgcctcaa gaacaagaa acgagggtt 
tttagtccg actgcttcc agtttgggg ccggaaagg 
gccaaacct aggagcagt ggaaggatt tcgctgaag 
aggaagagc ttcgctcaa gaagcagag ttttga
      mRNA sequence
      • >TC033829 RA
        ttaacccag tattcattt taccatgaa ggtttctgc 
ccgagttaa agacgagtg gttacaaat tccttgcaa 
agatggcac aaacacggt tagatggct aggagaaga 
agcatggag gaagtatca gaaattgat gcaaggggt 
caaaatcga agaacacga tataatcaa tgtcataca 
agaagtgag gaaaacgcg aggaggtgc tttattgga 
tcccgacga catcatctc tgaggtttt agataatga 
cgattttgt caacattgt tctaactac agacacgca 
agtcaatgt tggtgttat cccagagga gcttaaaca 
tgttttgga gccaattcc aagcaattt tctcccggc 
ggataatta cgtttttgt ggacggttc taacttaac 
tacggaaat gctggtgaa tttaggaaa tggagaatt 
taagattaa gttatcaaa agaagccat caatagcgt 
cctcaaatc tcgttcttt agtcgaatc ccttcttgg 
ggaagagcg ctcggttta cggcattac gacaggcat 
cggaagctt ctcagaagt cagcattcc gtatgataa 
gctcgaaga cctgcaata caacataat cagatcgca 
cgcggctgg cgtgggtaa tcctttaag ccccgcaag 
aactagaat gttgctcgc attaaggat taacgttct 
agcaaaggg ttacagcgg catatcctt aaacactct 
acagggcct cgtcgacgc atttaatgc ttcgtgctt 
atcgtgggt accggaaca gggatcagt gggcgccag 
cggcgactt ggccccttt agcccactt ggcgctcgg 
gattgtggg cgagggcaa gatgtggag ccccaaaac 
cggctgggg agaagccaa acgggtttt gaaactaaa 
tgggctgaa gcctctggt tctgggccc caaagaggc 
ggatgcact ggtcaatgg gacccaact tatcactag 
cattggggc cgaagctgt ggaaagggc tctgcatat 
agcaaaaca ggccgatgt tgttgctgc gcttacttt 
agaggtgtt gaaagggac gtctcgtgc gtttgattc 
agacgtcca gaagataag gccccacaa aggacagat 
cgctgtagc caaacgatt gagagcgct gcttcactc 
tgatactta tccgtccga aattgcaga aagccacag 
gttttgtaa taaggtcca agacgcata cactttgag 
atgttgtcc ccaggttca cggaattgt tcacgacac 
aatcgattt tgtacaagg aatcatcca gactgagat 
gaacagcgg gactgataa tcctcttgt ttttgctga 
tcgaggcga aatcatttc agcgggaaa tttccacgg 
ggaataccc tgccaaagt tttagactt cctaagtat 
agcggttca cgagttggc ttcaatgag cgaaaaacg 
aatcgagcg attagtaaa ccccgcatt aagcagcct 
tccggctta tctcgtgaa agatggagg cctcaattc 
cggtttcat ggtggcgca ttgcacggc tgcagcttt 
agtttctga aaacaaagt tgtttgcca cccttcgtc 
agtagacag tatgagcac gagcgccgg tcaggagga 
tcacgtttc gatgggatg tttcgctgc tcgaaaagc 
tctccaagt gatcgaaaa tgtggaaaa tgtgattgc 
tatcgagct ccttgcggc atgtcaagc aattgagtt 
tttgaggcc tttgaggac gacgacgcc tttggagga 
ggtttatgc tacagtgag gagtgtggt ccagccttg 
ggacaagga caggtttat ggcgcccga tattgaagc 
cgttaagaa gctcttagt tgagaataa aatttggaa 
tactgtgca acattatat gatgtttta tcatgcgcc 
tcaagaaca agaaacgag ggtttttag tccgactgc 
ttccagttt ggggccgga aagggccaa acctaggag 
cagtggaag gatttcgct gaagaggaa gagcttcgc 
tcaagaagc agagttttg atttattta cagtacacg 
tattgttat taattatta tcgttacca ataaactta 
tcatttgac g
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY