| General information | |||
| TC033829
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 9553585..9557185 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC033829 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04755 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)
- >TC033829 RA
MKVSARVKD EWLQIPCKD GTNTVRWLG EEAWRKYQK LMQGVKIEE HDIINVIQE VRKTRGGAL LDPDDIISE VLDNDDFVN IVLTTDTQV NVGVIPEEL KHVLEPIPS NFLPADNYV FVDGSNLTT EMLVNLGNG EFKIKLSKE AINSVLKSR SLVESLLGE ERSVYGITT GIGSFSEVS IPYDKLEDL QYNIIRSHA AGVGNPLSP ARTRMLLAL RINVLAKGY SGISLNTLQ GLVDAFNAS CLSWVPEQG SVGASGDLA PLAHLALGI VGEGKMWSP KTGWGEAKR VLKLNGLKP LVLGPKEAD ALVNGTQLI TSIGAEAVE RALHIAKQA DVVAALTLE VLKGTSRAF DSDVQKIRP HKGQIAVAK RLRALLHSD TYPSEIAES HRFCNKVQD AYTLRCCPQ VHGIVHDTI DFVQGIIQT EMNSGTDNP LVFADRGEI ISAGNFHGE YPAKVLDFL SIAVHELAS MSEKRIERL VNPALSSLP AYLVKDGGL NSGFMVAHC TAAALVSEN KVVCHPSSV DSMSTSAGQ EDHVSMGCF AARKALQVI ENVENVIAI ELLAACQAI EFLRPLRTT TPLEEVYAT VRSVVQPWD KDRFMAPDI EAVKKLLVE NKIWNTVQH YMMFYHAPQ EQETRVFSP TASSLGPER AKPRSSGRI SLKRKSFAQ EAEF
- >TC033829 RA
atgaaggtt tctgcccga gttaaagac gagtggtta caaattcct tgcaaagat ggcacaaac acggttaga tggctagga gaagaagca tggaggaag tatcagaaa ttgatgcaa ggggtcaaa atcgaagaa cacgatata atcaatgtc atacaagaa gtgaggaaa acgcgagga ggtgcttta ttggatccc gacgacatc atctctgag gttttagat aatgacgat tttgtcaac attgttcta actacagac acgcaagtc aatgttggt gttatccca gaggagctt aaacatgtt ttggagcca attccaagc aattttctc ccggcggat aattacgtt tttgtggac ggttctaac ttaactacg gaaatgctg gtgaattta ggaaatgga gaatttaag attaagtta tcaaaagaa gccatcaat agcgtcctc aaatctcgt tctttagtc gaatccctt cttggggaa gagcgctcg gtttacggc attacgaca ggcatcgga agcttctca gaagtcagc attccgtat gataagctc gaagacctg caatacaac ataatcaga tcgcacgcg gctggcgtg ggtaatcct ttaagcccc gcaagaact agaatgttg ctcgcatta aggattaac gttctagca aagggttac agcggcata tccttaaac actctacag ggcctcgtc gacgcattt aatgcttcg tgcttatcg tgggtaccg gaacaggga tcagtgggc gccagcggc gacttggcc cctttagcc cacttggcg ctcgggatt gtgggcgag ggcaagatg tggagcccc aaaaccggc tggggagaa gccaaacgg gttttgaaa ctaaatggg ctgaagcct ctggttctg ggccccaaa gaggcggat gcactggtc aatgggacc caacttatc actagcatt ggggccgaa gctgtggaa agggctctg catatagca aaacaggcc gatgttgtt gctgcgctt actttagag gtgttgaaa gggacgtct cgtgcgttt gattcagac gtccagaag ataaggccc cacaaagga cagatcgct gtagccaaa cgattgaga gcgctgctt cactctgat acttatccg tccgaaatt gcagaaagc cacaggttt tgtaataag gtccaagac gcatacact ttgagatgt tgtccccag gttcacgga attgttcac gacacaatc gattttgta caaggaatc atccagact gagatgaac agcgggact gataatcct cttgttttt gctgatcga ggcgaaatc atttcagcg ggaaatttc cacggggaa taccctgcc aaagtttta gacttccta agtatagcg gttcacgag ttggcttca atgagcgaa aaacgaatc gagcgatta gtaaacccc gcattaagc agccttccg gcttatctc gtgaaagat ggaggcctc aattccggt ttcatggtg gcgcattgc acggctgca gctttagtt tctgaaaac aaagttgtt tgccaccct tcgtcagta gacagtatg agcacgagc gccggtcag gaggatcac gtttcgatg ggatgtttc gctgctcga aaagctctc caagtgatc gaaaatgtg gaaaatgtg attgctatc gagctcctt gcggcatgt caagcaatt gagtttttg aggcctttg aggacgacg acgcctttg gaggaggtt tatgctaca gtgaggagt gtggtccag ccttgggac aaggacagg tttatggcg cccgatatt gaagccgtt aagaagctc ttagttgag aataaaatt tggaatact gtgcaacat tatatgatg ttttatcat gcgcctcaa gaacaagaa acgagggtt tttagtccg actgcttcc agtttgggg ccggaaagg gccaaacct aggagcagt ggaaggatt tcgctgaag aggaagagc ttcgctcaa gaagcagag ttttga
- >TC033829 RA
ttaacccag tattcattt taccatgaa ggtttctgc ccgagttaa agacgagtg gttacaaat tccttgcaa agatggcac aaacacggt tagatggct aggagaaga agcatggag gaagtatca gaaattgat gcaaggggt caaaatcga agaacacga tataatcaa tgtcataca agaagtgag gaaaacgcg aggaggtgc tttattgga tcccgacga catcatctc tgaggtttt agataatga cgattttgt caacattgt tctaactac agacacgca agtcaatgt tggtgttat cccagagga gcttaaaca tgttttgga gccaattcc aagcaattt tctcccggc ggataatta cgtttttgt ggacggttc taacttaac tacggaaat gctggtgaa tttaggaaa tggagaatt taagattaa gttatcaaa agaagccat caatagcgt cctcaaatc tcgttcttt agtcgaatc ccttcttgg ggaagagcg ctcggttta cggcattac gacaggcat cggaagctt ctcagaagt cagcattcc gtatgataa gctcgaaga cctgcaata caacataat cagatcgca cgcggctgg cgtgggtaa tcctttaag ccccgcaag aactagaat gttgctcgc attaaggat taacgttct agcaaaggg ttacagcgg catatcctt aaacactct acagggcct cgtcgacgc atttaatgc ttcgtgctt atcgtgggt accggaaca gggatcagt gggcgccag cggcgactt ggccccttt agcccactt ggcgctcgg gattgtggg cgagggcaa gatgtggag ccccaaaac cggctgggg agaagccaa acgggtttt gaaactaaa tgggctgaa gcctctggt tctgggccc caaagaggc ggatgcact ggtcaatgg gacccaact tatcactag cattggggc cgaagctgt ggaaagggc tctgcatat agcaaaaca ggccgatgt tgttgctgc gcttacttt agaggtgtt gaaagggac gtctcgtgc gtttgattc agacgtcca gaagataag gccccacaa aggacagat cgctgtagc caaacgatt gagagcgct gcttcactc tgatactta tccgtccga aattgcaga aagccacag gttttgtaa taaggtcca agacgcata cactttgag atgttgtcc ccaggttca cggaattgt tcacgacac aatcgattt tgtacaagg aatcatcca gactgagat gaacagcgg gactgataa tcctcttgt ttttgctga tcgaggcga aatcatttc agcgggaaa tttccacgg ggaataccc tgccaaagt tttagactt cctaagtat agcggttca cgagttggc ttcaatgag cgaaaaacg aatcgagcg attagtaaa ccccgcatt aagcagcct tccggctta tctcgtgaa agatggagg cctcaattc cggtttcat ggtggcgca ttgcacggc tgcagcttt agtttctga aaacaaagt tgtttgcca cccttcgtc agtagacag tatgagcac gagcgccgg tcaggagga tcacgtttc gatgggatg tttcgctgc tcgaaaagc tctccaagt gatcgaaaa tgtggaaaa tgtgattgc tatcgagct ccttgcggc atgtcaagc aattgagtt tttgaggcc tttgaggac gacgacgcc tttggagga ggtttatgc tacagtgag gagtgtggt ccagccttg ggacaagga caggtttat ggcgcccga tattgaagc cgttaagaa gctcttagt tgagaataa aatttggaa tactgtgca acattatat gatgtttta tcatgcgcc tcaagaaca agaaacgag ggtttttag tccgactgc ttccagttt ggggccgga aagggccaa acctaggag cagtggaag gatttcgct gaagaggaa gagcttcgc tcaagaagc agagttttg atttattta cagtacacg tattgttat taattatta tcgttacca ataaactta tcatttgac g
