Status
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Gene information
General information
TC033829
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010071,TC010072
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9553585..9557185
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC033829
      iBeetle screen iB_04755

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC033829 RA
        MKVSARVKD EWLQIPCKD GTNTVRWLG EEAWRKYQK 
        LMQGVKIEE HDIINVIQE VRKTRGGAL LDPDDIISE 
        VLDNDDFVN IVLTTDTQV NVGVIPEEL KHVLEPIPS 
        NFLPADNYV FVDGSNLTT EMLVNLGNG EFKIKLSKE 
        AINSVLKSR SLVESLLGE ERSVYGITT GIGSFSEVS 
        IPYDKLEDL QYNIIRSHA AGVGNPLSP ARTRMLLAL 
        RINVLAKGY SGISLNTLQ GLVDAFNAS CLSWVPEQG 
        SVGASGDLA PLAHLALGI VGEGKMWSP KTGWGEAKR 
        VLKLNGLKP LVLGPKEAD ALVNGTQLI TSIGAEAVE 
        RALHIAKQA DVVAALTLE VLKGTSRAF DSDVQKIRP 
        HKGQIAVAK RLRALLHSD TYPSEIAES HRFCNKVQD 
        AYTLRCCPQ VHGIVHDTI DFVQGIIQT EMNSGTDNP 
        LVFADRGEI ISAGNFHGE YPAKVLDFL SIAVHELAS 
        MSEKRIERL VNPALSSLP AYLVKDGGL NSGFMVAHC 
        TAAALVSEN KVVCHPSSV DSMSTSAGQ EDHVSMGCF 
        AARKALQVI ENVENVIAI ELLAACQAI EFLRPLRTT 
        TPLEEVYAT VRSVVQPWD KDRFMAPDI EAVKKLLVE 
        NKIWNTVQH YMMFYHAPQ EQETRVFSP TASSLGPER 
        AKPRSSGRI SLKRKSFAQ EAEF
      CDS sequence
      • >TC033829 RA
        atgaaggtt tctgcccga gttaaagac gagtggtta 
        caaattcct tgcaaagat ggcacaaac acggttaga 
        tggctagga gaagaagca tggaggaag tatcagaaa 
        ttgatgcaa ggggtcaaa atcgaagaa cacgatata 
        atcaatgtc atacaagaa gtgaggaaa acgcgagga 
        ggtgcttta ttggatccc gacgacatc atctctgag 
        gttttagat aatgacgat tttgtcaac attgttcta 
        actacagac acgcaagtc aatgttggt gttatccca 
        gaggagctt aaacatgtt ttggagcca attccaagc 
        aattttctc ccggcggat aattacgtt tttgtggac 
        ggttctaac ttaactacg gaaatgctg gtgaattta 
        ggaaatgga gaatttaag attaagtta tcaaaagaa 
        gccatcaat agcgtcctc aaatctcgt tctttagtc 
        gaatccctt cttggggaa gagcgctcg gtttacggc 
        attacgaca ggcatcgga agcttctca gaagtcagc 
        attccgtat gataagctc gaagacctg caatacaac 
        ataatcaga tcgcacgcg gctggcgtg ggtaatcct 
        ttaagcccc gcaagaact agaatgttg ctcgcatta 
        aggattaac gttctagca aagggttac agcggcata 
        tccttaaac actctacag ggcctcgtc gacgcattt 
        aatgcttcg tgcttatcg tgggtaccg gaacaggga 
        tcagtgggc gccagcggc gacttggcc cctttagcc 
        cacttggcg ctcgggatt gtgggcgag ggcaagatg 
        tggagcccc aaaaccggc tggggagaa gccaaacgg 
        gttttgaaa ctaaatggg ctgaagcct ctggttctg 
        ggccccaaa gaggcggat gcactggtc aatgggacc 
        caacttatc actagcatt ggggccgaa gctgtggaa 
        agggctctg catatagca aaacaggcc gatgttgtt 
        gctgcgctt actttagag gtgttgaaa gggacgtct 
        cgtgcgttt gattcagac gtccagaag ataaggccc 
        cacaaagga cagatcgct gtagccaaa cgattgaga 
        gcgctgctt cactctgat acttatccg tccgaaatt 
        gcagaaagc cacaggttt tgtaataag gtccaagac 
        gcatacact ttgagatgt tgtccccag gttcacgga 
        attgttcac gacacaatc gattttgta caaggaatc 
        atccagact gagatgaac agcgggact gataatcct 
        cttgttttt gctgatcga ggcgaaatc atttcagcg 
        ggaaatttc cacggggaa taccctgcc aaagtttta 
        gacttccta agtatagcg gttcacgag ttggcttca 
        atgagcgaa aaacgaatc gagcgatta gtaaacccc 
        gcattaagc agccttccg gcttatctc gtgaaagat 
        ggaggcctc aattccggt ttcatggtg gcgcattgc 
        acggctgca gctttagtt tctgaaaac aaagttgtt 
        tgccaccct tcgtcagta gacagtatg agcacgagc 
        gccggtcag gaggatcac gtttcgatg ggatgtttc 
        gctgctcga aaagctctc caagtgatc gaaaatgtg 
        gaaaatgtg attgctatc gagctcctt gcggcatgt 
        caagcaatt gagtttttg aggcctttg aggacgacg 
        acgcctttg gaggaggtt tatgctaca gtgaggagt 
        gtggtccag ccttgggac aaggacagg tttatggcg 
        cccgatatt gaagccgtt aagaagctc ttagttgag 
        aataaaatt tggaatact gtgcaacat tatatgatg 
        ttttatcat gcgcctcaa gaacaagaa acgagggtt 
        tttagtccg actgcttcc agtttgggg ccggaaagg 
        gccaaacct aggagcagt ggaaggatt tcgctgaag 
        aggaagagc ttcgctcaa gaagcagag ttttga
      mRNA sequence
      • >TC033829 RA
        ttaacccag tattcattt taccatgaa ggtttctgc 
        ccgagttaa agacgagtg gttacaaat tccttgcaa 
        agatggcac aaacacggt tagatggct aggagaaga 
        agcatggag gaagtatca gaaattgat gcaaggggt 
        caaaatcga agaacacga tataatcaa tgtcataca 
        agaagtgag gaaaacgcg aggaggtgc tttattgga 
        tcccgacga catcatctc tgaggtttt agataatga 
        cgattttgt caacattgt tctaactac agacacgca 
        agtcaatgt tggtgttat cccagagga gcttaaaca 
        tgttttgga gccaattcc aagcaattt tctcccggc 
        ggataatta cgtttttgt ggacggttc taacttaac 
        tacggaaat gctggtgaa tttaggaaa tggagaatt 
        taagattaa gttatcaaa agaagccat caatagcgt 
        cctcaaatc tcgttcttt agtcgaatc ccttcttgg 
        ggaagagcg ctcggttta cggcattac gacaggcat 
        cggaagctt ctcagaagt cagcattcc gtatgataa 
        gctcgaaga cctgcaata caacataat cagatcgca 
        cgcggctgg cgtgggtaa tcctttaag ccccgcaag 
        aactagaat gttgctcgc attaaggat taacgttct 
        agcaaaggg ttacagcgg catatcctt aaacactct 
        acagggcct cgtcgacgc atttaatgc ttcgtgctt 
        atcgtgggt accggaaca gggatcagt gggcgccag 
        cggcgactt ggccccttt agcccactt ggcgctcgg 
        gattgtggg cgagggcaa gatgtggag ccccaaaac 
        cggctgggg agaagccaa acgggtttt gaaactaaa 
        tgggctgaa gcctctggt tctgggccc caaagaggc 
        ggatgcact ggtcaatgg gacccaact tatcactag 
        cattggggc cgaagctgt ggaaagggc tctgcatat 
        agcaaaaca ggccgatgt tgttgctgc gcttacttt 
        agaggtgtt gaaagggac gtctcgtgc gtttgattc 
        agacgtcca gaagataag gccccacaa aggacagat 
        cgctgtagc caaacgatt gagagcgct gcttcactc 
        tgatactta tccgtccga aattgcaga aagccacag 
        gttttgtaa taaggtcca agacgcata cactttgag 
        atgttgtcc ccaggttca cggaattgt tcacgacac 
        aatcgattt tgtacaagg aatcatcca gactgagat 
        gaacagcgg gactgataa tcctcttgt ttttgctga 
        tcgaggcga aatcatttc agcgggaaa tttccacgg 
        ggaataccc tgccaaagt tttagactt cctaagtat 
        agcggttca cgagttggc ttcaatgag cgaaaaacg 
        aatcgagcg attagtaaa ccccgcatt aagcagcct 
        tccggctta tctcgtgaa agatggagg cctcaattc 
        cggtttcat ggtggcgca ttgcacggc tgcagcttt 
        agtttctga aaacaaagt tgtttgcca cccttcgtc 
        agtagacag tatgagcac gagcgccgg tcaggagga 
        tcacgtttc gatgggatg tttcgctgc tcgaaaagc 
        tctccaagt gatcgaaaa tgtggaaaa tgtgattgc 
        tatcgagct ccttgcggc atgtcaagc aattgagtt 
        tttgaggcc tttgaggac gacgacgcc tttggagga 
        ggtttatgc tacagtgag gagtgtggt ccagccttg 
        ggacaagga caggtttat ggcgcccga tattgaagc 
        cgttaagaa gctcttagt tgagaataa aatttggaa 
        tactgtgca acattatat gatgtttta tcatgcgcc 
        tcaagaaca agaaacgag ggtttttag tccgactgc 
        ttccagttt ggggccgga aagggccaa acctaggag 
        cagtggaag gatttcgct gaagaggaa gagcttcgc 
        tcaagaagc agagttttg atttattta cagtacacg 
        tattgttat taattatta tcgttacca ataaactta 
        tcatttgac g
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY