General information | |||
TC010062 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 9596880..9599715 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC010062 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_04754 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (16 dpi)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (16 dpi)
- >TC010062 RA
MDATEYRIR GKELVDYIA DYLEDIRER RVFPDKKPG FLRDLIPDS APIEGETWP RIFADIENV IMPGVTHWQ SPHMHAYFP ALTSYPSML GEMLSNAIN CVGFTWASS PAATELEMI VMNWLGKMI GLPENFLHR KNGSGGGVI QTTASEATL ISLLAGRWQ AIKKYKELY PDAENGEIN SKLVAYCSD QAHSSVEKA ALIGLVHIR YIECDENLS MRGPELLQT IKKDRELGF IPFWVCATL GTTGACSFD NLDEIGDVC VEEFLWLHV DAAYAGSAF ICPEYRVWL KGVEKVDSI SFNPSKWLM VHFDCTAMW VKNSLALHE AFNVDPVYL QHEYSGLAI DYMHWQIPL SKRFRAIKL WFVIRNYGI TGLQKHIRE GVRLAQKFE ALVQSDARF EIPAKRHLG LVVFRLVGD NIITETLWK KINSRGNIH CVPAIIKGK YIIRFTVTS PRTTNEDIV KDWQEIKTV ATEVLNEWT PGARPRVPL ADTKNRNEN FGTSLLLSN SPMSPKIVN GSFAAIYDR DDVIAEYSK TIKLRQDAQ NSPAMRRRI KGILMSGKQ FSLDSRLDL FHGIEAGTP SPSEGLPPL EDYNESEDN GSTFSGEFE PNEPNDDFL QVPNAPKQK RSKSVDHQV PISMQSEGY TTCKKCGHQ IITIQK
- >TC010062 RA
atggacgcc actgaatac cggatccgg ggcaaagaa ctcgtcgac tacattgcg gattatctg gaagacatc cgggagcgc agagtgttt ccagacaag aaacccgga ttcctccgg gacttgatt cccgacagc gctcccatc gagggcgaa acctggccc cggattttc gcagacata gaaaatgtc atcatgcca ggggtcacc cactggcag agtccccac atgcatgcg tacttcccg gctttgacg agctacccc tccatgctg ggggagatg ctctccaat gccatcaac tgtgtggga tttacttgg gcgtccagt ccggctgct accgaactg gagatgatc gtgatgaac tggctgggg aagatgatc ggtcttccg gagaatttt ttgcaccgg aaaaatggt agtggtggt ggggtcatc cagaccact gctagcgag gccaccttg atcagtcta ctggcaggt cgctggcag gcgattaag aagtacaag gagctttat ccggacgcc gaaaatggc gaaattaac agtaaatta gttgcttat tgttcggac caagctcac tccagcgta gaaaaagca gccttgatc ggtttagtc cacatccgg tatatcgaa tgcgacgag aacttatcg atgcgtgga cccgaactg ttacagact atcaagaaa gaccgcgag cttggattc ataccgttc tgggtttgt gctactttg ggcacgaca ggtgcttgc tcctttgat aatttagat gaaattgga gacgtttgt gtggaggag tttttgtgg ttgcatgtc gatgcagcg tacgctggg agcgccttt atatgccca gagtacaga gtttggcta aaaggtgtt gaaaaggtt gactcaatt tcctttaat ccatcgaaa tggctgatg gtacatttt gactgcacg gcaatgtgg gttaaaaat agtttagca cttcatgaa gccttcaac gtggacccg gtgtacctc caacacgaa tattcaggt ctagcgatt gactatatg cattggcag ataccgtta agtaagaga ttcagggcc attaaattg tggtttgta attcgcaat tacggcatt accggactt caaaaacac atacgcgag ggcgtgcgt ctcgctcaa aaattcgag gctctcgtc cagtcggac gcccgtttt gagatccca gccaagcgc catctaggg cttgttgtt tttcgccta gtgggcgac aacattatc actgaaact ctttggaaa aagatcaac tctcgagga aacatacac tgcgttccg gccataatc aagggcaaa tacataatc aggtttaca gtaacgtct ccgcgtacc acaaacgag gacattgtg aaagactgg caggaaatc aaaactgtg gcaactgaa gtcctcaac gagtggact cctggtgcc cggccaaga gtccccctt gcggatacc aagaacagg aacgagaat ttcgggaca agtctcttg ctttccaat tcgccaatg tctcccaaa attgtcaat ggaagtttt gcagccatt tacgaccgc gatgacgtg attgccgaa tactccaaa acgattaaa ttgagacaa gatgcacag aacagtcct gcaatgagg agaagaatc aaggggatt ttaatgtcg ggaaaacag ttttcttta gattcgaga ctagacttg ttccacggc atcgaagcc gggactcca agtcccagt gaaggctta ccgcctttg gaagattat aacgaaagt gaagacaac gggagcact ttttctgga gaattcgag ccgaatgaa cccaacgat gattttctc caagtgcca aatgcaccc aaacaaaaa cggtccaag tcggtggat catcaagtc ccaatttca atgcaaagt gagggttat acaacttgt aaaaaatgt gggcaccaa ataatcaca atccagaaa taa
- >TC010062 RA
ttcacgcac ccagatgga cgccactga ataccggat ccggggcaa agaactcgt cgactacat tgcggatta tctggaaga catccggga gcgcagagt gtttccaga caagaaacc cggattcct ccgggactt gattcccga cagcgctcc catcgaggg cgaaacctg gccccggat tttcgcaga catagaaaa tgtcatcat gccaggggt cacccactg gcagagtcc ccacatgca tgcgtactt cccggcttt gacgagcta cccctccat gctggggga gatgctctc caatgccat caactgtgt gggatttac ttgggcgtc cagtccggc tgctaccga actggagat gatcgtgat gaactggct ggggaagat gatcggtct tccggagaa ttttttgca ccggaaaaa tggtagtgg tggtggggt catccagac cactgctag cgaggccac cttgatcag tctactggc aggtcgctg gcaggcgat taagaagta caaggagct ttatccgga cgccgaaaa tggcgaaat taacagtaa attagttgc ttattgttc ggaccaagc tcactccag cgtagaaaa agcagcctt gatcggttt agtccacat ccggtatat cgaatgcga cgagaactt atcgatgcg tggacccga actgttaca gactatcaa gaaagaccg cgagcttgg attcatacc gttctgggt ttgtgctac tttgggcac gacaggtgc ttgctcctt tgataattt agatgaaat tggagacgt ttgtgtgga ggagttttt gtggttgca tgtcgatgc agcgtacgc tgggagcgc ctttatatg cccagagta cagagtttg gctaaaagg tgttgaaaa ggttgactc aatttcctt taatccatc gaaatggct gatggtaca ttttgactg cacggcaat gtgggttaa aaatagttt agcacttca tgaagcctt caacgtgga cccggtgta cctccaaca cgaatattc aggtctagc gattgacta tatgcattg gcagatacc gttaagtaa gagattcag ggccattaa attgtggtt tgtaattcg caattacgg cattaccgg acttcaaaa acacatacg cgagggcgt gcgtctcgc tcaaaaatt cgaggctct cgtccagtc ggacgcccg ttttgagat cccagccaa gcgccatct agggcttgt tgtttttcg cctagtggg cgacaacat tatcactga aactctttg gaaaaagat caactctcg aggaaacat acactgcgt tccggccat aatcaaggg caaatacat aatcaggtt tacagtaac gtctccgcg taccacaaa cgaggacat tgtgaaaga ctggcagga aatcaaaac tgtggcaac tgaagtcct caacgagtg gactcctgg tgcccggcc aagagtccc ccttgcgga taccaagaa caggaacga gaatttcgg gacaagtct cttgctttc caattcgcc aatgtctcc caaaattgt caatggaag ttttgcagc catttacga ccgcgatga cgtgattgc cgaatactc caaaacgat taaattgag acaagatgc acagaacag tcctgcaat gaggagaag aatcaaggg gattttaat gtcgggaaa acagttttc tttagattc gagactaga cttgttcca cggcatcga agccgggac tccaagtcc cagtgaagg cttaccgcc tttggaaga ttataacga aagtgaaga caacgggag cactttttc tggagaatt cgagccgaa tgaacccaa cgatgattt tctccaagt gccaaatgc acccaaaca aaaacggtc caagtcggt ggatcatca agtcccaat ttcaatgca aagtgaggg ttatacaac ttgtaaaaa atgtgggca ccaaataat cacaatcca gaaataagc tctttaaat gttgtagct tttctctct tgtgttgta acttgtaac ttttaaatt aaaggtaga cttactgct gtgctatgc aataattct cctaatgca acatagctg ttttagatc taagactgt atgcacgat ataaataaa ttgatgttg atgtt
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
