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Gene information
General information
TC010062 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9596880..9599715
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010062
    iBeetle screen iB_04754

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 4 number 2nd lateral oviduct: 3 - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010062 RA
      MDATEYRIR GKELVDYIA DYLEDIRER RVFPDKKPG 
      FLRDLIPDS APIEGETWP RIFADIENV IMPGVTHWQ 
      SPHMHAYFP ALTSYPSML GEMLSNAIN CVGFTWASS 
      PAATELEMI VMNWLGKMI GLPENFLHR KNGSGGGVI 
      QTTASEATL ISLLAGRWQ AIKKYKELY PDAENGEIN 
      SKLVAYCSD QAHSSVEKA ALIGLVHIR YIECDENLS 
      MRGPELLQT IKKDRELGF IPFWVCATL GTTGACSFD 
      NLDEIGDVC VEEFLWLHV DAAYAGSAF ICPEYRVWL 
      KGVEKVDSI SFNPSKWLM VHFDCTAMW VKNSLALHE 
      AFNVDPVYL QHEYSGLAI DYMHWQIPL SKRFRAIKL 
      WFVIRNYGI TGLQKHIRE GVRLAQKFE ALVQSDARF 
      EIPAKRHLG LVVFRLVGD NIITETLWK KINSRGNIH 
      CVPAIIKGK YIIRFTVTS PRTTNEDIV KDWQEIKTV 
      ATEVLNEWT PGARPRVPL ADTKNRNEN FGTSLLLSN 
      SPMSPKIVN GSFAAIYDR DDVIAEYSK TIKLRQDAQ 
      NSPAMRRRI KGILMSGKQ FSLDSRLDL FHGIEAGTP 
      SPSEGLPPL EDYNESEDN GSTFSGEFE PNEPNDDFL 
      QVPNAPKQK RSKSVDHQV PISMQSEGY TTCKKCGHQ 
      IITIQK
    CDS sequence
    • >TC010062 RA
      atggacgcc actgaatac cggatccgg ggcaaagaa 
      ctcgtcgac tacattgcg gattatctg gaagacatc 
      cgggagcgc agagtgttt ccagacaag aaacccgga 
      ttcctccgg gacttgatt cccgacagc gctcccatc 
      gagggcgaa acctggccc cggattttc gcagacata 
      gaaaatgtc atcatgcca ggggtcacc cactggcag 
      agtccccac atgcatgcg tacttcccg gctttgacg 
      agctacccc tccatgctg ggggagatg ctctccaat 
      gccatcaac tgtgtggga tttacttgg gcgtccagt 
      ccggctgct accgaactg gagatgatc gtgatgaac 
      tggctgggg aagatgatc ggtcttccg gagaatttt 
      ttgcaccgg aaaaatggt agtggtggt ggggtcatc 
      cagaccact gctagcgag gccaccttg atcagtcta 
      ctggcaggt cgctggcag gcgattaag aagtacaag 
      gagctttat ccggacgcc gaaaatggc gaaattaac 
      agtaaatta gttgcttat tgttcggac caagctcac 
      tccagcgta gaaaaagca gccttgatc ggtttagtc 
      cacatccgg tatatcgaa tgcgacgag aacttatcg 
      atgcgtgga cccgaactg ttacagact atcaagaaa 
      gaccgcgag cttggattc ataccgttc tgggtttgt 
      gctactttg ggcacgaca ggtgcttgc tcctttgat 
      aatttagat gaaattgga gacgtttgt gtggaggag 
      tttttgtgg ttgcatgtc gatgcagcg tacgctggg 
      agcgccttt atatgccca gagtacaga gtttggcta 
      aaaggtgtt gaaaaggtt gactcaatt tcctttaat 
      ccatcgaaa tggctgatg gtacatttt gactgcacg 
      gcaatgtgg gttaaaaat agtttagca cttcatgaa 
      gccttcaac gtggacccg gtgtacctc caacacgaa 
      tattcaggt ctagcgatt gactatatg cattggcag 
      ataccgtta agtaagaga ttcagggcc attaaattg 
      tggtttgta attcgcaat tacggcatt accggactt 
      caaaaacac atacgcgag ggcgtgcgt ctcgctcaa 
      aaattcgag gctctcgtc cagtcggac gcccgtttt 
      gagatccca gccaagcgc catctaggg cttgttgtt 
      tttcgccta gtgggcgac aacattatc actgaaact 
      ctttggaaa aagatcaac tctcgagga aacatacac 
      tgcgttccg gccataatc aagggcaaa tacataatc 
      aggtttaca gtaacgtct ccgcgtacc acaaacgag 
      gacattgtg aaagactgg caggaaatc aaaactgtg 
      gcaactgaa gtcctcaac gagtggact cctggtgcc 
      cggccaaga gtccccctt gcggatacc aagaacagg 
      aacgagaat ttcgggaca agtctcttg ctttccaat 
      tcgccaatg tctcccaaa attgtcaat ggaagtttt 
      gcagccatt tacgaccgc gatgacgtg attgccgaa 
      tactccaaa acgattaaa ttgagacaa gatgcacag 
      aacagtcct gcaatgagg agaagaatc aaggggatt 
      ttaatgtcg ggaaaacag ttttcttta gattcgaga 
      ctagacttg ttccacggc atcgaagcc gggactcca 
      agtcccagt gaaggctta ccgcctttg gaagattat 
      aacgaaagt gaagacaac gggagcact ttttctgga 
      gaattcgag ccgaatgaa cccaacgat gattttctc 
      caagtgcca aatgcaccc aaacaaaaa cggtccaag 
      tcggtggat catcaagtc ccaatttca atgcaaagt 
      gagggttat acaacttgt aaaaaatgt gggcaccaa 
      ataatcaca atccagaaa taa
    mRNA sequence
    • >TC010062 RA
      ttcacgcac ccagatgga cgccactga ataccggat 
      ccggggcaa agaactcgt cgactacat tgcggatta 
      tctggaaga catccggga gcgcagagt gtttccaga 
      caagaaacc cggattcct ccgggactt gattcccga 
      cagcgctcc catcgaggg cgaaacctg gccccggat 
      tttcgcaga catagaaaa tgtcatcat gccaggggt 
      cacccactg gcagagtcc ccacatgca tgcgtactt 
      cccggcttt gacgagcta cccctccat gctggggga 
      gatgctctc caatgccat caactgtgt gggatttac 
      ttgggcgtc cagtccggc tgctaccga actggagat 
      gatcgtgat gaactggct ggggaagat gatcggtct 
      tccggagaa ttttttgca ccggaaaaa tggtagtgg 
      tggtggggt catccagac cactgctag cgaggccac 
      cttgatcag tctactggc aggtcgctg gcaggcgat 
      taagaagta caaggagct ttatccgga cgccgaaaa 
      tggcgaaat taacagtaa attagttgc ttattgttc 
      ggaccaagc tcactccag cgtagaaaa agcagcctt 
      gatcggttt agtccacat ccggtatat cgaatgcga 
      cgagaactt atcgatgcg tggacccga actgttaca 
      gactatcaa gaaagaccg cgagcttgg attcatacc 
      gttctgggt ttgtgctac tttgggcac gacaggtgc 
      ttgctcctt tgataattt agatgaaat tggagacgt 
      ttgtgtgga ggagttttt gtggttgca tgtcgatgc 
      agcgtacgc tgggagcgc ctttatatg cccagagta 
      cagagtttg gctaaaagg tgttgaaaa ggttgactc 
      aatttcctt taatccatc gaaatggct gatggtaca 
      ttttgactg cacggcaat gtgggttaa aaatagttt 
      agcacttca tgaagcctt caacgtgga cccggtgta 
      cctccaaca cgaatattc aggtctagc gattgacta 
      tatgcattg gcagatacc gttaagtaa gagattcag 
      ggccattaa attgtggtt tgtaattcg caattacgg 
      cattaccgg acttcaaaa acacatacg cgagggcgt 
      gcgtctcgc tcaaaaatt cgaggctct cgtccagtc 
      ggacgcccg ttttgagat cccagccaa gcgccatct 
      agggcttgt tgtttttcg cctagtggg cgacaacat 
      tatcactga aactctttg gaaaaagat caactctcg 
      aggaaacat acactgcgt tccggccat aatcaaggg 
      caaatacat aatcaggtt tacagtaac gtctccgcg 
      taccacaaa cgaggacat tgtgaaaga ctggcagga 
      aatcaaaac tgtggcaac tgaagtcct caacgagtg 
      gactcctgg tgcccggcc aagagtccc ccttgcgga 
      taccaagaa caggaacga gaatttcgg gacaagtct 
      cttgctttc caattcgcc aatgtctcc caaaattgt 
      caatggaag ttttgcagc catttacga ccgcgatga 
      cgtgattgc cgaatactc caaaacgat taaattgag 
      acaagatgc acagaacag tcctgcaat gaggagaag 
      aatcaaggg gattttaat gtcgggaaa acagttttc 
      tttagattc gagactaga cttgttcca cggcatcga 
      agccgggac tccaagtcc cagtgaagg cttaccgcc 
      tttggaaga ttataacga aagtgaaga caacgggag 
      cactttttc tggagaatt cgagccgaa tgaacccaa 
      cgatgattt tctccaagt gccaaatgc acccaaaca 
      aaaacggtc caagtcggt ggatcatca agtcccaat 
      ttcaatgca aagtgaggg ttatacaac ttgtaaaaa 
      atgtgggca ccaaataat cacaatcca gaaataagc 
      tctttaaat gttgtagct tttctctct tgtgttgta 
      acttgtaac ttttaaatt aaaggtaga cttactgct 
      gtgctatgc aataattct cctaatgca acatagctg 
      ttttagatc taagactgt atgcacgat ataaataaa 
      ttgatgttg atgtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY