Status
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Gene information
General information
TC010059 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9649671..9652565
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010059
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04753

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    mature egg deposition blocked number 1st lateral oviduct: 4 number 2nd lateral oviduct: 3 - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 3
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010059 RA
      MSEEGVQHA QKSERFKRK RKKSFLKNA RKYAKKGCY 
      GRGSQLDAD TYQYFVRIM EAYRQGFDS DEDKAIFVN 
      NVFEQTVDQ EINCCCNQV GCRVIEMLI PFANDEVLK 
      RYMKCFGDE LRPLCCDRF ASHVIESLV AEAAKKSFK 
      PISKDDINA SCKEFVLKV SRFLLNNLE DYLWDTYGN 
      HVIRRCLES LAQIPNEKV KNDAKSEEP DIKVELPEE 
      FIEVIKDYC ERIMHWPQF GELCQTDVT SGFLQSLLK 
      ALKKADKKL LKKLLEKLL STNFAPEVE NSDSDNLPS 
      VFLSKPAMM VLETTLEVS KSKMFTQIF VKCFSGRLV 
      KLATTRSTN FTVQKLIMN CKEKSEFEL MFDELSSNF 
      KEIIVAGHT GIIWALAQS CKNLASKQG SFMQNLMKS 
      LDCVEEEKH KDFILCLCK FNDFDTTQK QSKENLQKD 
      KLSLHGTLI LQLLLEFNK PIKIVNGLL SLDQNELKA 
      LFSNTMGSH IVDSYVKSL YVGEKSREK LIRKMKGTY 
      QELASSKFG SRSFEAIWN AANLKNKLA IMEELVYKD 
      GSWSSSQYG KIIANKINL ALYKRNKEE WKNSLNTVK 
      KAEKLFEDI LK
    CDS sequence
    • >TC010059 RA
      atgtctgaa gaaggtgtc caacatgct caaaaaagt 
      gagcgtttc aaacgaaaa cgaaaaaaa tcatttttg 
      aaaaacgct agaaaatac gcgaaaaaa ggctgttac 
      ggtcgagga agtcaactg gatgctgat acttaccaa 
      tatttcgtt cgaattatg gaagcttac agacagggc 
      tttgattca gatgaggat aaagcaatt ttcgtaaat 
      aacgtattt gagcaaact gttgatcag gaaattaac 
      tgctgttgc aaccaggtg ggatgtcgg gttatcgaa 
      atgttgata ccttttgcc aatgatgaa gtgttgaaa 
      agatacatg aagtgtttt ggggacgag cttagaccc 
      ttgtgttgt gaccgattt gcgtcacat gtgattgaa 
      tccttggtg gcagaagca gccaagaag tcatttaaa 
      ccaatttct aaagatgac attaatgct agttgtaaa 
      gaatttgtt ttaaaagta agcagattt ttgcttaat 
      aatttggaa gactatcta tgggacaca tatgggaac 
      catgttatt agaaggtgt ttggagagt ttagcacaa 
      attccaaac gaaaaagtg aaaaatgat gctaaatct 
      gaagaacct gacataaaa gttgaactc ccagaagag 
      ttcattgaa gtcattaaa gactactgt gaaaggata 
      atgcactgg ccccagttt ggcgagttg tgccaaacc 
      gacgtcact tccgggttt ttgcaatct ttactaaaa 
      gcactcaaa aaggccgac aaaaaatta ctcaaaaaa 
      ctgctagag aaattgctt tcgaccaat tttgcacca 
      gaagttgaa aacagcgac agcgataat ctcccttct 
      gtgtttttg tctaaaccg gcaatgatg gtcctagag 
      actacttta gaagtttcc aaatcaaaa atgtttact 
      cagatattt gtgaagtgt ttttctggc cgacttgtt 
      aaattggcg actacaaga agtactaac tttaccgtg 
      caaaaatta ataatgaat tgtaaagaa aaatcagag 
      tttgagtta atgtttgat gaactgtcc agcaacttt 
      aaagaaatt atcgtagca ggtcacaca ggcataatc 
      tgggctctc gcccaaagt tgcaagaat ttggcctca 
      aagcaaggc agttttatg caaaacttg atgaagagt 
      ttggattgt gttgaagaa gaaaagcac aaagatttc 
      attttgtgt ttatgcaaa tttaatgat tttgataca 
      actcagaaa cagtcaaag gagaatctc caaaaagat 
      aaactgagt ttgcatggg accctaatt cttcaatta 
      ctactagag tttaataag ccaattaaa attgtcaat 
      ggtttatta agtttggac caaaatgaa ctcaaagcc 
      ttgttttcg aacacaatg ggtagccac atcgttgat 
      tcctacgtc aaaagtttg tatgtgggc gaaaaaagc 
      cgtgaaaaa ttaattagg aagatgaag gggacttac 
      caagagttg gcatcgtct aaattcggt tcaaggtca 
      tttgaagcg atctggaat gctgccaac ttgaaaaat 
      aaattggcg attatggaa gaactggtc tataaagac 
      gggtcatgg tctagttca cagtacggt aaaatcatt 
      gcgaataaa ataaatctc gctttatat aaacgtaac 
      aaagaagag tggaagaac tctcttaat accgtcaaa 
      aaagccgag aaattgttt gaagatatt ctgaaataa
    mRNA sequence
    • >TC010059 RA
      tttctgatt cgaatttag aagcataac ctctgtttt 
      tccacctca ttgtttcgt ttttatttc ttcttttta 
      ttaaacgta ttgactaaa aagcgtgac tatgtctga 
      agaaggtgt ccaacatgc tcaaaaaag tgagcgttt 
      caaacgaaa acgaaaaaa atcattttt gaaaaacgc 
      tagaaaata cgcgaaaaa aggctgtta cggtcgagg 
      aagtcaact ggatgctga tacttacca atatttcgt 
      tcgaattat ggaagctta cagacaggg ctttgattc 
      agatgagga taaagcaat tttcgtaaa taacgtatt 
      tgagcaaac tgttgatca ggaaattaa ctgctgttg 
      caaccaggt gggatgtcg ggttatcga aatgttgat 
      accttttgc caatgatga agtgttgaa aagatacat 
      gaagtgttt tggggacga gcttagacc cttgtgttg 
      tgaccgatt tgcgtcaca tgtgattga atccttggt 
      ggcagaagc agccaagaa gtcatttaa accaatttc 
      taaagatga cattaatgc tagttgtaa agaatttgt 
      tttaaaagt aagcagatt tttgcttaa taatttgga 
      agactatct atgggacac atatgggaa ccatgttat 
      tagaaggtg tttggagag tttagcaca aattccaaa 
      cgaaaaagt gaaaaatga tgctaaatc tgaagaacc 
      tgacataaa agttgaact cccagaaga gttcattga 
      agtcattaa agactactg tgaaaggat aatgcactg 
      gccccagtt tggcgagtt gtgccaaac cgacgtcac 
      ttccgggtt tttgcaatc tttactaaa agcactcaa 
      aaaggccga caaaaaatt actcaaaaa actgctaga 
      gaaattgct ttcgaccaa ttttgcacc agaagttga 
      aaacagcga cagcgataa tctcccttc tgtgttttt 
      gtctaaacc ggcaatgat ggtcctaga gactacttt 
      agaagtttc caaatcaaa aatgtttac tcagatatt 
      tgtgaagtg tttttctgg ccgacttgt taaattggc 
      gactacaag aagtactaa ctttaccgt gcaaaaatt 
      aataatgaa ttgtaaaga aaaatcaga gtttgagtt 
      aatgtttga tgaactgtc cagcaactt taaagaaat 
      tatcgtagc aggtcacac aggcataat ctgggctct 
      cgcccaaag ttgcaagaa tttggcctc aaagcaagg 
      cagttttat gcaaaactt gatgaagag tttggattg 
      tgttgaaga agaaaagca caaagattt cattttgtg 
      tttatgcaa atttaatga ttttgatac aactcagaa 
      acagtcaaa ggagaatct ccaaaaaga taaactgag 
      tttgcatgg gaccctaat tcttcaatt actactaga 
      gtttaataa gccaattaa aattgtcaa tggtttatt 
      aagtttgga ccaaaatga actcaaagc cttgttttc 
      gaacacaat gggtagcca catcgttga ttcctacgt 
      caaaagttt gtatgtggg cgaaaaaag ccgtgaaaa 
      attaattag gaagatgaa ggggactta ccaagagtt 
      ggcatcgtc taaattcgg ttcaaggtc atttgaagc 
      gatctggaa tgctgccaa cttgaaaaa taaattggc 
      gattatgga agaactggt ctataaaga cgggtcatg 
      gtctagttc acagtacgg taaaatcat tgcgaataa 
      aataaatct cgctttata taaacgtaa caaagaaga 
      gtggaagaa ctctcttaa taccgtcaa aaaagccga 
      gaaattgtt tgaagatat tctgaaata atgcaacaa 
      atatgtgaa tttttatat tgggaaagt acatcagtt 
      ttaaaatat aaataaaat aaataataa acactaaat 
      aacatggaa atgtacaat aatgaacta cctgcctaa 
      cgttttata cacgcacgt ttggaagcc aatttacta 
      aagtaattg cttttatca aatttaatt tttttgtta 
      gtaaattgg ttcccaaaa aattgtaaa agtgactag 
      attattatt acaacaaaa ctggacaaa gataaatcg 
      atcaacacc tcggatact gtgtgcatc tatttatct 
      ttgaattac ttgaaagtc gaagagacc agaatttga 
      aacgaaagt gatgatgcg aacggtaag tgcggcata 
      cggaaatct tatatttca ccatgtcag aaaacctca 
      ttgcagtgt atccatatc ttgaaaaga gatttctaa 
      ccccgaaaa ctttttggt aaaattatc tagcagaca 
      aactacaaa gtgtttatc tagtgagaa gggcactaa 
      aacaggtga ctacaaatc aaacgttcc ttttttgtt 
      agtttcgtt tacgttaca agattgcgc aacatttga 
      tacaggtga ggaacaaga caattaaat taggggaac 
      agagagcat taatcatat ttaatatga gaatagaaa 
      gtttttttc ccgaaaata atattaatt tacaaattt 
      tttatcaac ttgcgatcc atcccaaat ttttataac 
      gtctcctat cttattttt aatagtttg gctttaaat 
      tgcttctct gggacgata tggttctcc aaatccctt 
      ctcaacaaa tttctctgt tacatttgt aaaaaatat 
      agagtggaa cacaaattt atatttatt aatatattt 
      tgtaaagaa aa
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