Status
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Gene information
General information
TC010050 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9744495..9748631
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010050
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04752

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    eggs lost during procedure: potentially due to defects in egg-shells egg-morphology or egg-size, (14 dpi)
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010050 RA
      MDQIAENYQ LKWHSFGSY LHSCIATSL QNETFADVA 
      LVTIDGRQI MAHRFVLSA CSQYLHQVL KLQPRVTTA 
      LPLMIILPP EINYRTMKT LIQYMYSGE ATVSKDILE 
      PVLRGGDIL KVKGLWRPK EDESNENRL VKVSHKNKS 
      SKTEQKEAQ ESATSPGNS AAPKVLNYT INVMKTYDG 
      QKEGAKDKQ SQVKSGEQE KEKPKQNNQ NSVIKSTED 
      EKRTQKSTE SVDSEKTEQ SNDSLQFLV IKEEPIEWS 
      EVNESDMEL VDEKEVFST EMTIKPEIY MEDGEEQGL 
      YSPLTCELC SETFTLPAE WVRHVQTHT DMLPAKRQR 
      RGKSLQDED SDAPFPQLH CDLCQKYFP TPAEWVHHI 
      QNTHTEFEL HLSNKTAPS KVIKTPKSF GPAQNCCTI 
      CSKKFPSHA SLLIHKRTH TGEKPYMCE LCFKGFNVK 
      SNLLRHLRT VHDKIINPT EVDDGIKDE TTGD
    CDS sequence
    • >TC010050 RA
      atggaccaa atcgccgaa aactaccaa ctgaaatgg 
      cactcgttc ggctcctat ctccactcg tgcatcgca 
      acgtctctc cagaacgaa acttttgcc gatgtggct 
      cttgtcaca atagacggt cgccagata atggcccac 
      cgtttcgtc ctatccgcc tgcagccaa tacctccac 
      caagtcctc aaattacaa ccccgcgtg accacggct 
      ctcccgttg atgatcatc ctccctccc gaaatcaat 
      taccgaacc atgaaaacc ctcatccag tatatgtac 
      agtggagaa gccaccgtt tccaaagac atcctcgag 
      ccggttctc cgaggtggc gacatcctc aaagttaag 
      ggcttatgg cgtccgaaa gaggacgaa agtaacgaa 
      aatcggcta gtgaaggta tcgcacaaa aataagtct 
      agcaagacc gaacagaaa gaagctcaa gagtctgcg 
      acaagtccg ggaaattcg gcggcgccg aaggttctg 
      aactatact attaatgtg atgaagacg tacgacggc 
      cagaaggaa ggggcgaaa gacaagcaa agtcaggtc 
      aagagtggt gaacaggag aaggagaag cctaagcaa 
      aacaaccag aattcggtc atcaagagt acggaggac 
      gagaaaagg acgcagaaa agcacagaa tcggttgat 
      agtgagaag actgagcaa agcaacgac tcgcttcag 
      ttcctggtg attaaggag gagccgatc gagtggagc 
      gaagtcaac gagtccgac atggagcta gtggacgag 
      aaagaggtc ttcagcacg gaaatgacc atcaaaccg 
      gaaatctac atggaggat ggtgaagag caaggcttg 
      tattcaccc ttgacctgc gagctttgc tcggagact 
      ttcacgctt ccagccgag tgggtgcgc cacgtgcag 
      acccacacg gacatgttg ccggcaaag aggcaaaga 
      cgaggcaaa agtttacag gacgaagac tcagacgcg 
      ccttttcct cagctccac tgcgacctc tgtcaaaag 
      tacttccct acccctgcc gaatgggtg caccacatc 
      cagaatacg catacggag ttcgagctg cacctgtcg 
      aacaaaacc gcgcccagt aaagtgata aagacgcca 
      aagtcgttc ggccccgct cagaattgt tgcacgatc 
      tgttccaaa aagtttcct tctcatgct tccttgttg 
      atacataaa cgcacgcac accggcgag aaaccgtac 
      atgtgtgaa ttgtgtttt aaagggttt aacgttaag 
      agtaatctt ttaaggcat ttgaggact gtgcatgac 
      aagattatc aatccgacc gaggtggat gacgggatc 
      aaagacgag acaacgggg gattga
    mRNA sequence
    • >TC010050 RA
      ctgcagccg acggtgatg cgttcgtgt tgaccgcct 
      tgaaacgct gaaagacac catggacca aatcgccga 
      aaactacca actgaaatg gcactcgtt cggctccta 
      tctccactc gtgcatcgc aacgtctct ccagaacga 
      aacttttgc cgatgtggc tcttgtcac aatagacgg 
      tcgccagat aatggccca ccgtttcgt cctatccgc 
      ctgcagcca atacctcca ccaagtcct caaattaca 
      accccgcgt gaccacggc tctcccgtt gatgatcat 
      cctccctcc cgaaatcaa ttaccgaac catgaaaac 
      cctcatcca gtatatgta cagtggaga agccaccgt 
      ttccaaaga catcctcga gccggttct ccgaggtgg 
      cgacatcct caaagttaa gggcttatg gcgtccgaa 
      agaggacga aagtaacga aaatcggct agtgaaggt 
      atcgcacaa aaataagtc tagcaagac cgaacagaa 
      agaagctca agagtctgc gacaagtcc gggaaattc 
      ggcggcgcc gaaggttct gaactatac tattaatgt 
      gatgaagac gtacgacgg ccagaagga aggggcgaa 
      agacaagca aagtcaggt caagagtgg tgaacagga 
      gaaggagaa gcctaagca aaacaacca gaattcggt 
      catcaagag tacggagga cgagaaaag gacgcagaa 
      aagcacaga atcggttga tagtgagaa gactgagca 
      aagcaacga ctcgcttca gttcctggt gattaagga 
      ggagccgat cgagtggag cgaagtcaa cgagtccga 
      catggagct agtggacga gaaagaggt cttcagcac 
      ggaaatgac catcaaacc ggaaatcta catggagga 
      tggtgaaga gcaaggctt gtattcacc cttgacctg 
      cgagctttg ctcggagac tttcacgct tccagccga 
      gtgggtgcg ccacgtgca gacccacac ggacatgtt 
      gccggcaaa gaggcaaag acgaggcaa aagtttaca 
      ggacgaaga ctcagacgc gccttttcc tcagctcca 
      ctgcgacct ctgtcaaaa gtacttccc tacccctgc 
      cgaatgggt gcaccacat ccagaatac gcatacgga 
      gttcgagct gcacctgtc gaacaaaac cgcgcccag 
      taaagtgat aaagacgcc aaagtcgtt cggccccgc 
      tcagaattg ttgcacgat ctgttccaa aaagtttcc 
      ttctcatgc ttccttgtt gatacataa acgcacgca 
      caccggcga gaaaccgta catgtgtga attgtgttt 
      taaagggtt taacgttaa gagtaatct tttaaggca 
      tttgaggac tgtgcatga caagattat caatccgac 
      cgaggtgga tgacgggat caaagacga gacaacggg 
      ggattgagt cttgatgca ttttatgtg tacataaat 
      cgttctatt ctatattct taataaaat ttaatcgac 
      tca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY