Status
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Gene information
General information
TC010040 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9911096..9916580
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010040
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04749

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010040 RA
      MVLSTEWSQ VEIIDLVND GSGLGFGIV GGRSTGVVI 
      KSILPGGIA DKDSRLQSG DHILQIGDV NLRGLAADQ 
      VATVLRQAG AQVRMVVAR PVEPSSADF QSFGCSAPI 
      VPTKILTDP EELDRQLQQ NGYSSFYAY NDRGSCDDL 
      EQTDVNSID ANPASFNSL DVVVNNNNN TNGFSPTSE 
      SQVVIIPAD IEYTLPETE RFTVELTKN EYGLGITIA 
      GYVCEREDL SGIFVKSLN EGSEAFKCG KINTNDRIV 
      EVDGQSLQG FTNYEAVEK IKQTGGKVV LTLERYLRG 
      PKFEQLQEA LAIQEQKDL SPPSPSQTT LSWIPIEAA 
      EGQIEPEGE SVVSVNSEI YEQNLETKE IFIEEDFEA 
      NPDDDLEPP PRKMGEAII GSDTEIVVA NLTKLKGLG 
      ISLEGTVDV EGGIELRPH HYIRSILPE GPVGQNGKL 
      SSGDELLEV NGQKLLGIK HVEVVKILR ELPSTVRLV 
      CARKHEENR VINTSQDRE AFEARNILG GSLKNLLPQ 
      PEQRLLKAL SDTSINTSS TVTVTSEPS LQKAKSRSL 
      EVTNLAMWS EEVEYVELL KTDRGLGFS ILDYQDPLD 
      PKATVIVVR SLVPNGAAE HDGRITPGD RLISVNGKV 
      IKNVTLDQA VQALKGTLP GPVKLGISK PLPSSRASE 
      NVSTIGS
    CDS sequence
    • >TC010040 RA
      atggtgttg agcacggaa tggtcgcaa gttgagatt 
      atcgacttg gtgaacgat ggctcgggg ctggggttc 
      ggcatcgtc ggcggcaga agcacgggg gtcgtcata 
      aagagcatc ctcccaggg ggcatcgcc gacaaagac 
      agccgtctc cagagcggc gaccacatc ctccagatc 
      ggagacgtc aatttaagg gggctcgcc gccgaccaa 
      gttgccacc gttcttagg caggctggg gcacaggtc 
      aggatggtg gtcgcacgt cctgttgag ccttcttcc 
      gcggatttc cagtccttc ggctgttcg gctcccata 
      gttccgaca aaaatcctc accgatccg gaagagctc 
      gaccgccag ctccaacaa aacggctac tcctctttc 
      tacgcttac aacgaccgc ggttcttgc gacgacctc 
      gaacaaacc gacgtaaat tccatcgat gctaacccc 
      gcaagtttc aattcttta gacgtggtt gtcaacaac 
      aataataac acgaatggc ttctcgccc accagcgag 
      agtcaagtg gtgatcatc cccgccgac attgaatac 
      actcttcca gaaacggag cgcttcacc gtagaactg 
      acaaaaaac gaatacggg ctggggatt acgatagca 
      gggtacgtg tgcgagagg gaggatctc agtggaatc 
      ttcgtcaag agtttgaac gaaggcagc gaagccttt 
      aagtgcgga aagattaat actaacgat aggattgtg 
      gaagttgat ggacagtct ttgcaaggg tttacgaat 
      tacgaggct gtggagaag attaagcaa acgggaggg 
      aaagttgtg ttgactttg gagaggtac ttgagaggg 
      cccaagttc gagcaatta caagaagcg ttagctatt 
      caggaacaa aaggatttg agtccacct tcgccttcg 
      caaacgact ctcagttgg atacctatt gaagcagcc 
      gagggccag atcgagccg gaaggtgag tccgtcgtg 
      agcgtcaat agcgaaatc tacgagcaa aacctcgaa 
      actaaagaa atctttatc gaagaagac ttcgaggcg 
      aaccccgat gacgatcta gaaccgcca ccaagaaaa 
      atgggggag gctataatc ggcagcgac actgaaatc 
      gtcgtcgcc aacctgaca aaactcaaa ggcttgggc 
      ataagcctg gagggaaca gtcgacgtg gaaggaggc 
      atcgaatta cgtccccat cactacatc cgttccata 
      ctccccgaa ggccccgtc ggccaaaac ggcaaactc 
      agctcaggt gacgaactc ctggaagta aacggccaa 
      aaactcctc gggatcaag cacgtggaa gtcgtcaag 
      atcctccgc gagttgccg agcaccgtc cgcctggtc 
      tgtgctcgc aagcacgag gagaaccgc gtcatcaac 
      acctcccaa gaccgcgaa gctttcgaa gcgcggaac 
      atcctgggg ggcagtttg aaaaacctc ttaccccaa 
      ccggaacaa cgcctgctc aaagctcta agcgacacg 
      agtatcaac acttcgagc accgtgacc gtgaccagc 
      gagcccagc ttgcaaaaa gccaaatcg cgctcgctg 
      gaagtgacg aacttggcg atgtggtcg gaggaggtc 
      gagtatgtc gagttgctc aaaaccgat cggggtttg 
      ggcttctcg atcctggac taccaggac cctctggac 
      cccaaagcg accgtcata gtcgtgcgg agtctcgtg 
      cctaacggg gcggcggag cacgacggc cgcatcact 
      ccaggtgat cgactgatc agtgttaat ggcaaagta 
      atcaaaaat gtgaccctg gaccaggcg gtccaggcg 
      ctgaaaggg acactgccc ggtcctgtc aagttaggt 
      atctccaaa ccgttaccc agttccaga gccagcgaa 
      aacgtcagt acgataggg tcttga
    mRNA sequence
    • >TC010040 RA
      tatggcctc gttcagata accacaaaa acacttggt 
      gccagtctc cgcgatgtc tgcccgact tcggatcga 
      caaaaaagt gattgccgg cgtgccgtt cacgcttta 
      tcaaccgtc tcggacata acctaatcg gccaccgtt 
      ggaagagcg ccaagttcg aaggggccc ctttgagag 
      atgcggcta gagcgagga catcatggt gttgagcac 
      ggaatggtc gcaagttga gattatcga cttggtgaa 
      cgatggctc ggggctggg gttcggcat cgtcggcgg 
      cagaagcac gggggtcgt cataaagag catcctccc 
      agggggcat cgccgacaa agacagccg tctccagag 
      cggcgacca catcctcca gatcggaga cgtcaattt 
      aagggggct cgccgccga ccaagttgc caccgttct 
      taggcaggc tggggcaca ggtcaggat ggtggtcgc 
      acgtcctgt tgagccttc ttccgcgga tttccagtc 
      cttcggctg ttcggctcc catagttcc gacaaaaat 
      cctcaccga tccggaaga gctcgaccg ccagctcca 
      acaaaacgg ctactcctc tttctacgc ttacaacga 
      ccgcggttc ttgcgacga cctcgaaca aaccgacgt 
      aaattccat cgatgctaa ccccgcaag tttcaattc 
      tttagacgt ggttgtcaa caacaataa taacacgaa 
      tggcttctc gcccaccag cgagagtca agtggtgat 
      catccccgc cgacattga atacactct tccagaaac 
      ggagcgctt caccgtaga actgacaaa aaacgaata 
      cgggctggg gattacgat agcagggta cgtgtgcga 
      gagggagga tctcagtgg aatcttcgt caagagttt 
      gaacgaagg cagcgaagc ctttaagtg cggaaagat 
      taatactaa cgataggat tgtggaagt tgatggaca 
      gtctttgca agggtttac gaattacga ggctgtgga 
      gaagattaa gcaaacggg agggaaagt tgtgttgac 
      tttggagag gtacttgag agggcccaa gttcgagca 
      attacaaga agcgttagc tattcagga acaaaagga 
      tttgagtcc accttcgcc ttcgcaaac gactctcag 
      ttggatacc tattgaagc agccgaggg ccagatcga 
      gccggaagg tgagtccgt cgtgagcgt caatagcga 
      aatctacga gcaaaacct cgaaactaa agaaatctt 
      tatcgaaga agacttcga ggcgaaccc cgatgacga 
      tctagaacc gccaccaag aaaaatggg ggaggctat 
      aatcggcag cgacactga aatcgtcgt cgccaacct 
      gacaaaact caaaggctt gggcataag cctggaggg 
      aacagtcga cgtggaagg aggcatcga attacgtcc 
      ccatcacta catccgttc catactccc cgaaggccc 
      cgtcggcca aaacggcaa actcagctc aggtgacga 
      actcctgga agtaaacgg ccaaaaact cctcgggat 
      caagcacgt ggaagtcgt caagatcct ccgcgagtt 
      gccgagcac cgtccgcct ggtctgtgc tcgcaagca 
      cgaggagaa ccgcgtcat caacacctc ccaagaccg 
      cgaagcttt cgaagcgcg gaacatcct ggggggcag 
      tttgaaaaa cctcttacc ccaaccgga acaacgcct 
      gctcaaagc tctaagcga cacgagtat caacacttc 
      gagcaccgt gaccgtgac cagcgagcc cagcttgca 
      aaaagccaa atcgcgctc gctggaagt gacgaactt 
      ggcgatgtg gtcggagga ggtcgagta tgtcgagtt 
      gctcaaaac cgatcgggg tttgggctt ctcgatcct 
      ggactacca ggaccctct ggaccccaa agcgaccgt 
      catagtcgt gcggagtct cgtgcctaa cggggcggc 
      ggagcacga cggccgcat cactccagg tgatcgact 
      gatcagtgt taatggcaa agtaatcaa aaatgtgac 
      cctggacca ggcggtcca ggcgctgaa agggacact 
      gcccggtcc tgtcaagtt aggtatctc caaaccgtt 
      acccagttc cagagccag cgaaaacgt cagtacgat 
      agggtcttg aagcgacaa tttttcgac aattgtaaa 
      taacaatat tttagtact tggattttg gataagcac 
      aatcactgc tgccttttc gaatatttt tccgtagtt 
      aaggtgatt agatgacga atttataag ctgtaaggc 
      ttcaagggc acaaattcg tatgtattt tatacgcat 
      atcagctga tgaggtttt aaagtgtcc agacacttc 
      actttttta acgctctag tcgctaggt atatcagga 
      attaatgaa ggtaggcga tagacgggg gaagtcccc 
      gcttgtata gtccggagt ccggtcact agtgcttcg 
      atcagtttt cttctacaa gcaggttct tccataaat 
      caatacgta acaatggaa tttatgcac aatactgat 
      ctaaataag gaattgaat acacttgat gttttactt 
      acactgtgt aaataattg tgtggtgtt atgtgttac 
      aagtctgaa tcttgatat tcgtgccat gttttgttg 
      tgattttgt ttttgttaa gactgattt taaacaact 
      caatctgga ggtcaatat ttaagcaat atggcggtt 
      attatgtga taagttcat tggcctaag tttttaatt 
      ttggtgtgt gatgtgtac ataaaaaat cgatcgtat 
      tgtaaaaat aggataaat aataaaata cttgattcg 
      agttaaaac tcgagacgg aatatttat cgattcttg 
      ccagagttg atgaagagt tcctcctca aattgttaa 
      atagagtca ctggggggt ggttttgat ttgttgcat 
      gattaaatt aggtcccac cggaaatta ctattctta 
      gacactgtt caagcttca ttatttaca aaaaactat 
      cctctaatc ataattaca ataaattat gtaagtgac 
      g
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