Status
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Gene information
General information
TC033802
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010021,TC010022
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 10092242..10109216
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033802
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04747

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 4 - Number of animals showing the phenotype: 3
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033802 RA
      MLRWLGPRK APTPNDGQA DPIPSILQD EDVTDDPLA 
      VLDTHRKGL KQIYSVSRS VFLPRVSLK NTCNTDNDV 
      CCAPPGVNP LFRDAPQKA PRQRSPQPG EKSSSSGYG 
      SSLDQFYSD PKLRDESEQ NQNFAKSVK PNRVGKNNR 
      SPPSSGSSS GTLTTIPEG RVDLPIPKP IWPDRKPHD 
      SLVDDALLR YTSLNQEQD FTTDRLDVS VKEVLVELL 
      NGINATMDG STGLTPEEM LKSVNEKIA ASIELLRLT 
      TEEEMKKLC VNLSNCRKI NSVLRAFSN SPERTVSDA 
      DELYQIPSG SSSSGFSDL HALPVFIHE DLGSVPNGV 
      RNAMIYGTL YRGKNLGSK PAKDAPPKR TSLRDVNDN 
      KPSVWEQYY GVRAVQEGE VKYAAKPTD VPLFPGGRP 
      EADFTLDVP RSELLSKRM KQDKKWRCR CRLLTSFLG 
      LVFFLLSVM AVSLMLTRG KRMFGSMM
    CDS sequence
    • >TC033802 RA
      atgttaaga tggcttgga ccgcgcaag gccccgacg 
      ccgaacgac ggtcaagca gatcctatc ccgagcatc 
      ctccaagac gaagacgtc accgacgac cccctcgct 
      gtcctagac acccaccgg aagggactc aagcagatc 
      tactcagtc agcaggtca gtcttcttg cccagagtt 
      agtctcaag aacacttgt aacactgac aatgacgtt 
      tgctgtgcg ccgccgggg gtaaacccc ctttttcgc 
      gacgctcca caaaaagcc ccccggcaa cgcagcccc 
      caaccaggc gaaaagtcc agctcgtcg gggtatggg 
      agcagtctc gaccagttc tactccgac ccaaaactc 
      cgtgatgaa agtgaacaa aaccaaaat tttgccaag 
      tcagtcaaa cccaaccgg gttggtaaa aacaaccgg 
      tcgccccca tcatctgga tcctcctct ggtaccttg 
      accaccatt cctgaaggt cgcgtcgac ctccccatc 
      cccaagccc atctggccg gacaggaaa ccccacgat 
      tccctcgtc gatgacgcg ctcctccgc tacaccagc 
      ctcaaccaa gagcaggac tttaccacc gaccgtcta 
      gacgtctcc gtcaaagag gtcctcgtg gagctcctc 
      aacgggatc aacgccacc atggacgga agcaccgga 
      ctaacccct gaagaaatg ctgaagtcc gtcaacgag 
      aaaatcgcc gctagcatc gaactcctg cgcttgacc 
      accgaggaa gaaatgaag aaactgtgc gttaacttg 
      agcaactgt cggaaaatc aattcggtc ctccgggct 
      ttcagtaac tctcccgag cgaactgtg agtgacgcc 
      gacgagttg taccagatt ccctcgggg tcgtcgagt 
      agtggtttt agtgatttg cacgctctc ccggttttc 
      atccacgag gacctgggg agtgtgccg aacggggtg 
      aggaacgcg atgatctac gggactctg taccggggc 
      aagaacctc gggagcaag cccgcgaag gacgcgccg 
      ccgaagagg acctccctg agggacgtc aacgacaat 
      aagccgagc gtgtgggag cagtactac ggggtgagg 
      gccgtgcag gaaggggag gtcaaatat gcggcaaaa 
      ccaacggat gtcccgctt tttcctggg ggccggccc 
      gaggcggac ttcaccctg gacgttccg cggtcggag 
      ctgctgtcc aaacgcatg aagcaggac aagaagtgg 
      cgatgtcgc tgtcggctg ctcacctcc ttcctgggg 
      ctggtcttt ttcctgctg tctgtgatg gctgtgagt 
      ctgatgctc acccgaggc aagcgcatg ttcggttcg 
      atgatgtga
    mRNA sequence
    • >TC033802 RA
      tgccggctg catgcgcga gaccttcgc caaagacca 
      agttatgtg ctgagcaag tgacagttc gttgacaga 
      agcccaaaa ccgagctgt taaggccgt cacatctcg 
      cactgtcat gctgaaagg tagctgaaa tccgccgaa 
      tcagaaatg ttaagatgg cttggaccg cgcaaggcc 
      ccgacgccg aacgacggt caagcagat cctatcccg 
      agcatcctc caagacgaa gacgtcacc gacgacccc 
      ctcgctgtc ctagacacc caccggaag ggactcaag 
      cagatctac tcagtcagc aggtcagtc ttcttgccc 
      agagttagt ctcaagaac acttgtaac actgacaat 
      gacgtttgc tgtgcgccg ccgggggta aaccccctt 
      tttcgcgac gctccacaa aaagccccc cggcaacgc 
      agcccccaa ccaggcgaa aagtccagc tcgtcgggg 
      tatgggagc agtctcgac cagttctac tccgaccca 
      aaactccgt gatgaaagt gaacaaaac caaaatttt 
      gccaagtca gtcaaaccc aaccgggtt ggtaaaaac 
      aaccggtcg cccccatca tctggatcc tcctctggt 
      accttgacc accattcct gaaggtcgc gtcgacctc 
      cccatcccc aagcccatc tggccggac aggaaaccc 
      cacgattcc ctcgtcgat gacgcgctc ctccgctac 
      accagcctc aaccaagag caggacttt accaccgac 
      cgtctagac gtctccgtc aaagaggtc ctcgtggag 
      ctcctcaac gggatcaac gccaccatg gacggaagc 
      accggacta acccctgaa gaaatgctg aagtccgtc 
      aacgagaaa atcgccgct agcatcgaa ctcctgcgc 
      ttgaccacc gaggaagaa atgaagaaa ctgtgcgtt 
      aacttgagc aactgtcgg aaaatcaat tcggtcctc 
      cgggctttc agtaactct cccgagcga actgtgagt 
      gacgccgac gagttgtac cagattccc tcggggtcg 
      tcgagtagt ggttttagt gatttgcac gctctcccg 
      gttttcatc cacgaggac ctggggagt gtgccgaac 
      ggggtgagg aacgcgatg atctacggg actctgtac 
      cggggcaag aacctcggg agcaagccc gcgaaggac 
      gcgccgccg aagaggacc tccctgagg gacgtcaac 
      gacaataag ccgagcgtg tgggagcag tactacggg 
      gtgagggcc gtgcaggaa ggggaggtc aaatatgcg 
      gcaaaacca acggatgtc ccgcttttt cctgggggc 
      cggcccgag gcggacttc accctggac gttccgcgg 
      tcggagctg ctgtccaaa cgcatgaag caggacaag 
      aagtggcga tgtcgctgt cggctgctc acctccttc 
      ctggggctg gtctttttc ctgctgtct gtgatggct 
      gtgagtctg atgctcacc cgaggcaag cgcatgttc 
      ggttcgatg atgtgacca aaatttttg tgatattcg 
      acgttttat cacattgat gcaccgaaa tactgccat 
      aagtttgct cattttgca ggtgccaaa tgctagtta 
      ccacgattt tgcagtatt agtcctgat gggctatta 
      gagttgtga taaaattat agtgtcatg taggtatta 
      tgtctttag agtgattgt aagtcgacc tttgccacg 
      aatcggggg atttggggc aactggagg cattatttt 
      tctttaaaa acacattct taacttatt gattcaggt 
      atttttcat ttattttta agaaatgta tttaactag 
      atatgttgc aagtttgag tgtaagtta atgtgtaaa 
      tgatgtgtc gaaaatgat ttgtgtgca aattattgt 
      atatattgt aataaataa acaaataaa ctgtta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY