Status
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Gene information
General information
TC010011 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 10387264..10391147
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010011
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04744

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva appendages partially shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head appendages partially shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    legs shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    legs tibiotarsus shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    legs femur shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    legs femur potentially fused - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    legs trochanter potentially fused - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010011 RA
      MVRKTQTKS PGKKSRKSD PDISRSDVM FTSYSETSV 
      HKRNLCIVS FPIIFLFNI LRTLLYQIF VILRFVYCS 
      SSNYLVKKR QFECGESDQ QVATCVIEE ITEMTHVQR 
      VTGPGPGDP LLAKQKHHH RRAFEYISK ALKIDEENE 
      GQKDLAIEL YRKGITELE LGIAVQCWG GRGEVWERA 
      QRLHEKMKT NLAMAKDRL QFLESMDML HRQDVQKEP 
      KEAPKLSYT TNIPTPKLC FNKASGKKL SVASKRPPN 
      ATLAKSQTL PRSMGSRTA PIQPVRPFN KLSSTPPAV 
      KKQLSVPGT AGSPARKTT ASLATPSKS TSRGGKSPN 
      LRGVDTKLA QCILDEIVE GGLQVQWED IIGQDAAKQ 
      ALQEMVILP SLRPELFTG LRTPARGLL LFGPPGNGK 
      TLLARAVAT ECRATFFSI SAASLTSKY VGEGEKMVR 
      ALFAIAREL QPSIIFIDE VDSLLSERS NNEHEASRR 
      LKTEFLVEF DGLPSNPDS ERVVVMAAT NRPQELDEA 
      ALRRFPKRV YVTLPDLET RIRLFKMLL AKQGCSLTQ 
      QELKRLATL TEGYSASDL TALAKDAAL GPIRELQPE 
      QVKEMDPSA LRSITINDF LDSLKRIRR SVSPQSLVA 
      YEKWSLQYG DMSI
    CDS sequence
    • >TC010011 RA
      atggtgcgt aaaacccaa acgaaatca cctggcaaa 
      aaatcaaga aaaagtgac cccgacatt tcgagaagc 
      gatgtgatg tttacgtcg tactcagag acctctgtc 
      cacaagcga aacctatgc atcgtttcc ttcccgatt 
      atttttctg ttcaacatt ttgcgcact ttgctctac 
      caaatcttt gttatcctt cgtttcgtg tattgtagt 
      tcctcaaat tacctagta aagaaacgt cagtttgaa 
      tgtggtgag agtgaccaa caagttgcg acttgtgtc 
      attgaagag atcaccgaa atgacgcac gtccagagg 
      gtcacaggg ccggggccc ggggacccc cttctggcc 
      aagcagaag caccaccac cgccgcgcc ttcgagtac 
      atctccaag gcgctcaag atcgatgaa gagaacgaa 
      ggtcagaag gacttggcc atcgagttg tataggaaa 
      ggcatcacg gagctggaa ctggggatc gcggtgcag 
      tgctggggc ggccggggc gaggtgtgg gagcgggcc 
      cagcgcctc cacgaaaaa atgaaaacc aatcttgcc 
      atggccaag gacaggctg cagtttttg gaaagcatg 
      gatatgtta catcgacag gatgttcaa aaagaacca 
      aaagaggct cctaaactt agctacact accaatatt 
      cctactccc aaactttgt tttaacaaa gcttccggc 
      aagaaactc tccgtggcc agtaagcgc cccccgaac 
      gcgaccctg gccaaaagc caaaccttg ccccgttcg 
      atgggttcc cggacagcc cctattcag cccgtccgc 
      ccttttaac aagctctcg tcgactcct cctgccgtc 
      aaaaagcaa ctctcggtg ccgggcacc gccggttct 
      cccgctcgc aaaacaaca gcctcacta gcaactccg 
      agtaaatcg acgtcacgg ggcggcaaa tcacccaat 
      ttacgaggc gtcgacaca aaactagct caatgcatc 
      cttgacgag atcgtcgaa ggcggcctc caggtgcaa 
      tgggaggac atcatcggt caagacgcc gccaaacaa 
      gcgctgcaa gaaatggtg atcttgccg tcgctgcgg 
      cctgaactg ttcacgggg ctgcgcacc cccgcgcgg 
      gggctgctc ctgttcggg cccccaggc aacgggaag 
      acgttgcta gcgagggct gtggccacc gaatgtcgg 
      gcgacgttt ttctcaatc agtgccgcc agtctcact 
      tcgaagtac gtgggcgaa ggggagaaa atggtgcgg 
      gctttgttc gcaatcgcc cgggagttg caaccgtcg 
      attattttc atcgacgaa gtcgattcg ctcctctcc 
      gaacgctcc aacaacgag catgaggct agccgcagg 
      ttgaagacc gaatttctg gtcgagttc gacggactg 
      ccgagtaac cccgacagc gagcgggtc gtggtgatg 
      gcggcgacc aaccgcccc caggaactg gacgaagcc 
      gctctccga aggttcccg aagcgcgtc tacgtcacg 
      ctccccgat ctcgaaacg cgaattcga ttgtttaag 
      atgttgttg gcaaaacag ggttgttca cttacgcaa 
      caggaactg aaacgactg gccactctt actgaagga 
      tactcggct agtgattta acagctctg gccaaggat 
      gccgcgctg ggaccaatc agggagctg cagccggag 
      caagtgaag gagatggat cccagtgcg ctgaggagc 
      atcactata aatgatttt ctggattcg ctgaaacgg 
      atcaggagg agtgtgtcg ccgcagagc ttggtggcg 
      tacgagaag tggtcgttg cagtacgga gatatgtct 
      atttaa
    mRNA sequence
    • >TC010011 RA
      tattatgaa aaaaaaagt tggcagccc tgtaacagc 
      gtgagtgtg atggcatcc ggatggtgc gtaaaaccc 
      aaacgaaat cacctggca aaaaatcaa gaaaaagtg 
      accccgaca tttcgagaa gcgatgtga tgtttacgt 
      cgtactcag agacctctg tccacaagc gaaacctat 
      gcatcgttt ccttcccga ttatttttc tgttcaaca 
      ttttgcgca ctttgctct accaaatct ttgttatcc 
      ttcgtttcg tgtattgta gttcctcaa attacctag 
      taaagaaac gtcagtttg aatgtggtg agagtgacc 
      aacaagttg cgacttgtg tcattgaag agatcaccg 
      aaatgacgc acgtccaga gggtcacag ggccggggc 
      ccggggacc cccttctgg ccaagcaga agcaccacc 
      accgccgcg ccttcgagt acatctcca aggcgctca 
      agatcgatg aagagaacg aaggtcaga aggacttgg 
      ccatcgagt tgtatagga aaggcatca cggagctgg 
      aactgggga tcgcggtgc agtgctggg gcggccggg 
      gcgaggtgt gggagcggg cccagcgcc tccacgaaa 
      aaatgaaaa ccaatcttg ccatggcca aggacaggc 
      tgcagtttt tggaaagca tggatatgt tacatcgac 
      aggatgttc aaaaagaac caaaagagg ctcctaaac 
      ttagctaca ctaccaata ttcctactc ccaaacttt 
      gttttaaca aagcttccg gcaagaaac tctccgtgg 
      ccagtaagc gccccccga acgcgaccc tggccaaaa 
      gccaaacct tgccccgtt cgatgggtt cccggacag 
      cccctattc agcccgtcc gccctttta acaagctct 
      cgtcgactc ctcctgccg tcaaaaagc aactctcgg 
      tgccgggca ccgccggtt ctcccgctc gcaaaacaa 
      cagcctcac tagcaactc cgagtaaat cgacgtcac 
      ggggcggca aatcaccca atttacgag gcgtcgaca 
      caaaactag ctcaatgca tccttgacg agatcgtcg 
      aaggcggcc tccaggtgc aatgggagg acatcatcg 
      gtcaagacg ccgccaaac aagcgctgc aagaaatgg 
      tgatcttgc cgtcgctgc ggcctgaac tgttcacgg 
      ggctgcgca cccccgcgc gggggctgc tcctgttcg 
      ggcccccag gcaacggga agacgttgc tagcgaggg 
      ctgtggcca ccgaatgtc gggcgacgt ttttctcaa 
      tcagtgccg ccagtctca cttcgaagt acgtgggcg 
      aaggggaga aaatggtgc gggctttgt tcgcaatcg 
      cccgggagt tgcaaccgt cgattattt tcatcgacg 
      aagtcgatt cgctcctct ccgaacgct ccaacaacg 
      agcatgagg ctagccgca ggttgaaga ccgaatttc 
      tggtcgagt tcgacggac tgccgagta accccgaca 
      gcgagcggg tcgtggtga tggcggcga ccaaccgcc 
      cccaggaac tggacgaag ccgctctcc gaaggttcc 
      cgaagcgcg tctacgtca cgctccccg atctcgaaa 
      cgcgaattc gattgttta agatgttgt tggcaaaac 
      agggttgtt cacttacgc aacaggaac tgaaacgac 
      tggccactc ttactgaag gatactcgg ctagtgatt 
      taacagctc tggccaagg atgccgcgc tgggaccaa 
      tcagggagc tgcagccgg agcaagtga aggagatgg 
      atcccagtg cgctgagga gcatcacta taaatgatt 
      ttctggatt cgctgaaac ggatcagga ggagtgtgt 
      cgccgcaga gcttggtgg cgtacgaga agtggtcgt 
      tgcagtacg gagatatgt ctatttaat cattccgct 
      tttactttg gtgactttc gttttaatc tgtgatatt 
      atggtgttt ttgattgaa aattcgctg cctgttgat 
      tttgttgac ttgttttag tacaaaagg gagggagtt 
      tctcttttt atttgagcc gaaatgacg tattaaaat 
      attgaagta atgtagtta gactaggag tgtttttag 
      agtttgtgt ttactagca cgtttttta ttacttgat 
      ttaacattg ttttgtggt tgttcactt ggaccgtaa 
      caaaagtgt ctcaattca gacgttgct ttttccatg 
      acgtttgta atcttattg gtataataa aagtaaatt 
      gaaaga
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