Status
Please wait ...
Gene information
General information
TC010010 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 10396603..10404797
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010010
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04743

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    2 larva on slide

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 10 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010010 RA
      MAYHYVVTA HKPTAVTAC VTGNFTSPG DLNLIVAKN 
      TKLEIYLVT PEGLRPVKE VALYGKVAV MKLFRPQHE 
      KKDLLFIVT MRYNAMILE CVNDADNLD IITKAHGNV 
      ADKIGKPSE TGILAVIDP KARVIGLRL YDGLFKIIP 
      LEKDNSELK ASNIRIDEL QVHDVEFLH GCANPTLIL 
      IHQDVNGRH VKTHEISLR EKEFVKVPW RQDNVETEA 
      SMIIPVPSP LGGAIIIGQ ENILYHDGI TPVVVAPAV 
      IKQSTIVCY AKVDPGGLR YLLGDMAGH LFMLFLEVD 
      NRGDGNDVV KDLKVELLG EIATPECIT YLDNGVLFI 
      GSRLGDSQL VKLTTKPNE SGSYVTVME SFTNLAPIL 
      DMCVVDLER QGQGQLVTC SGAFKEGSL RIIRNGIGI 
      QEHASIDLP GIKGMWALQ VASDGRYDN TLVLAFVGQ 
      TRVLSLNGE EVEETDIAG FASDQQTFF CGNVIHEQI 
      VQITPISAR LISAQNKTL LAEWKPPSD KNIAVVACN 
      TCQIVVSTG SILYYIEIH QNELIMKGV TTLDVEVAC 
      LDISPLGDG VTTSEYIAV GLWTDITAR ILRIPDLSE 
      ATKEYLGGE IIPRSVLMT CFEGHSYLL CALGDGSMF 
      YFVFHKDTG TLSDKKRVT LGTQPTVLR TFRSLSTTN 
      VFACSDRPT VIYSSNHKL VFSNVNMKE VNHMCSLNA 
      EAYPDSLAL ATDSSVTIG TIDEIQKLH IRTVPLQES 
      PKRIAYQEE SQTFGVLTA RIDIQDSTG LNPARPSAS 
      TMAQSTTSS SSVGSLSMS KSGSSSLIG GNIIPDYGQ 
      EVEVHNLLI IDQHTFEVL HAHQLMQQE YAMSIISTN 
      RLGGDMNEY YIVGTATVN PEESEPKQG RILIFQWND 
      NKLTQVSEK EIKGACYSL AEFNGKLLA SINSTVRLF 
      EWTVEKELR LECSHFNNI LTLFLKTKG DFILLGDLM 
      RSMTLLQYK TMEGSFEEI ARDYNPNWM TAVEILDDD 
      IFLGAENSF NIFVCQKDS AATTDEERS QMHEVGRFH 
      VGDMINVFR HGSLVMQNL GETSTPTTG CVLFGTVSG 
      AIGLVTQIT QDFYDFLLE LQNKLSTVI KSVGKIDHS 
      QWRAFNTDI KTEPSEGFI DGDLIESFL DLSHDKMKE 
      VADGLQITG EGGMKQDCT VDDLVKMVE DLTRIH
    CDS sequence
    • >TC010010 RA
      atggcttac cattacgtc gtcacggcc cacaagcca 
      acggctgta acggcctgt gttacaggc aactttact 
      tcccccggc gatttgaac ttaattgtg gccaaaaac 
      acaaaattg gagatatat ttagtcacc cccgaaggt 
      ctcaggcct gtcaaggaa gtcgcactg tatgggaaa 
      gttgccgtt atgaaatta ttcagaccc caacatgag 
      aagaaggat ttattgttt attgtgact atgcgttac 
      aatgctatg atccttgaa tgtgtgaat gatgccgat 
      aatttggat attatcact aaagcccac ggtaatgtt 
      gctgataaa atcgggaag ccatcagag acaggcatc 
      ttggccgtg attgacccc aaggcccga gtgatcggt 
      ttacgtctc tacgatggg ctgtttaaa attatacct 
      ctggaaaag gacaattct gaactaaaa gcgtcaaat 
      ataagaatt gatgagttg caagtgcat gacgtggag 
      ttcctccat ggttgtgcc aaccccacc ttgattctc 
      atccaccaa gacgtaaac gggcgtcat gtcaaaacc 
      cacgaaatc tccctcaga gaaaaagag tttgttaaa 
      gtcccttgg aggcaagac aatgtcgaa actgaggcc 
      tccatgatc atccctgtg ccatcgccg ctagggggt 
      gccataata atcggccaa gaaaacatc ctctaccac 
      gatggtatc actcccgtg gtcgtggcc cctgctgtt 
      atcaaacag agcaccatc gtatgctac gccaaggtt 
      gaccccggg ggcctgcgc tacctcctc ggtgacatg 
      gccgggcac cttttcatg ctatttctg gaagtggat 
      aatcggggc gacggtaac gacgtcgtt aaagacttg 
      aaggtggaa ttgttgggg gaaatcgcg acgcctgaa 
      tgcattacg tacttggat aatggagtt ttatttatt 
      ggatcgaga ttgggcgac tcgcagttg gtgaaactg 
      accactaaa ccaaacgag agtgggtcg tacgttacc 
      gtgatggag tcttttacg aatttggcg ccgattttg 
      gatatgtgt gtcgttgat ttggagaga caggggcag 
      ggacagttg gtgacgtgc tcgggggcc ttcaaggag 
      ggctccttg aggattata aggaatggt attgggatt 
      caggagcat gcgtcgatt gatttgccg gggattaag 
      ggcatgtgg gcgctgcaa gtggcatca gatggccgt 
      tacgacaat accttagtc ctcgctttc gtgggccaa 
      actcgcgtc ttgagcttg aacggtgag gaagttgaa 
      gaaacggac attgctggc ttcgcctcc gaccagcag 
      actttcttt tgcggcaac gtcatccac gaacaaatc 
      gtacaaatt accccaatc tctgcccgt ctaatatca 
      gctcaaaac aaaactttg ttggcggaa tggaaaccg 
      cctagcgat aaaaacata gcagtagtg gcatgcaac 
      acgtgccaa atagtggtt tcgaccggc tctattttg 
      tattatatt gaaatccac caaaatgaa ctgatcatg 
      aaaggcgtg accacactt gacgttgag gtggcttgt 
      ttggacata agccctctg ggggacggt gtaacaacc 
      tccgaatat atcgctgtg gggctctgg accgatatc 
      acagcccgg attttgcgc atacccgac ttgtctgaa 
      gccacaaag gagtattta gggggagaa attataccc 
      cgctcggtt ctaatgacc tgtttcgag ggtcatagc 
      tatttgttg tgtgccttg ggtgacggt tcaatgttc 
      tatttcgtg tttcacaaa gatacggga acgttaagt 
      gacaaaaaa cgcgtcact ttgggtacc caaccgact 
      gttttacgt accttccgc tcgttgagc actacgaat 
      gtttttgct tgttcggat agaccgact gtaatttat 
      tcatccaat cacaagctc gttttttct aacgttaac 
      atgaaggaa gttaatcat atgtgttcg cttaatgcc 
      gaggcatat ccggacagt ttagcttta gcaacagat 
      tcgtccgtc actattggg acaatcgat gagatacag 
      aagctacat attaggacg gtgccactg caggagtct 
      ccaaagaga atagcgtac caggaggag tcacagact 
      tttggtgtt ctgacggcc cgcatcgac atccaagac 
      tccaccggc ttgaacccg gctcgtccg agcgcttca 
      acgatggct cagtcgaca acatcttca agcagtgtc 
      gggtctctc tcaatgagt aaatcaggg agcagcagt 
      ttgatcggt ggtaacata attcccgac tacggccaa 
      gaagtcgaa gtccacaac ttgctcatt atcgatcaa 
      cacaccttc gaagtattg catgcccac caattgatg 
      cagcaagaa tatgcaatg tcgataatc tcaaccaat 
      cgcctaggt ggcgacatg aacgaatac tacatagtt 
      ggcactgcc acagtcaat ccagaagaa tcggaaccc 
      aagcaaggc cgcatctta attttccag tggaacgac 
      aataaacta acccaagtg tctgagaaa gaaatcaag 
      ggggcttgc tactccctt gcggagttc aacggcaaa 
      ctcctggcg agtatcaac agcacggtg aggctgttc 
      gagtggacg gtggaaaag gagctgcga ttggagtgc 
      tcccacttt aataatatt ctaactctg tttttgaaa 
      accaaaggc gattttatc ctcttgggt gacttgatg 
      cgcagcatg accttgctg cagtacaaa acaatggaa 
      ggtagcttt gaagaaatc gcgagggat tataacccg 
      aactggatg acggctgtt gaaatactc gatgacgac 
      attttccta ggggctgag aattcgttt aacatattc 
      gtttgtcag aaagacagt gccgctacg acggacgag 
      gaaaggtcg caaatgcac gaagtgggg cggtttcac 
      gtcggggac atgatcaac gtcttcaga cacggatct 
      ctagttatg caaaatctg ggagagact tcgacgccg 
      accacaggc tgtgtcttg tttggcaca gtcagtgga 
      gccataggt ttagtgacg cagataacc caagatttt 
      tacgacttt ctcctagag ctgcaaaat aaactatcg 
      acggttatt aaatcggtc ggtaaaatt gatcactcc 
      caatggaga gcgttcaac accgatatt aaaacagag 
      ccgagtgaa ggcttcatt gacggtgac ttgattgag 
      agtttcctc gatttgtcg cacgacaag atgaaggaa 
      gtggccgat ggattacag ataactggg gaaggtggc 
      atgaaacag gattgtacc gtcgatgat ttagtcaag 
      atggtcgaa gatttaacg aggattcac taa
    mRNA sequence
    • >TC010010 RA
      atacgaaaa atgatattt tgcgtcatt tagataatt 
      ttaagcaat aaatctcgt ggaatttgc agaatggct 
      taccattac gtcgtcacg gcccacaag ccaacggct 
      gtaacggcc tgtgttaca ggcaacttt acttccccc 
      ggcgatttg aacttaatt gtggccaaa aacacaaaa 
      ttggagata tatttagtc acccccgaa ggtctcagg 
      cctgtcaag gaagtcgca ctgtatggg aaagttgcc 
      gttatgaaa ttattcaga ccccaacat gagaagaag 
      gatttattg tttattgtg actatgcgt tacaatgct 
      atgatcctt gaatgtgtg aatgatgcc gataatttg 
      gatattatc actaaagcc cacggtaat gttgctgat 
      aaaatcggg aagccatca gagacaggc atcttggcc 
      gtgattgac cccaaggcc cgagtgatc ggtttacgt 
      ctctacgat gggctgttt aaaattata cctctggaa 
      aaggacaat tctgaacta aaagcgtca aatataaga 
      attgatgag ttgcaagtg catgacgtg gagttcctc 
      catggttgt gccaacccc accttgatt ctcatccac 
      caagacgta aacgggcgt catgtcaaa acccacgaa 
      atctccctc agagaaaaa gagtttgtt aaagtccct 
      tggaggcaa gacaatgtc gaaactgag gcctccatg 
      atcatccct gtgccatcg ccgctaggg ggtgccata 
      ataatcggc caagaaaac atcctctac cacgatggt 
      atcactccc gtggtcgtg gcccctgct gttatcaaa 
      cagagcacc atcgtatgc tacgccaag gttgacccc 
      gggggcctg cgctacctc ctcggtgac atggccggg 
      caccttttc atgctattt ctggaagtg gataatcgg 
      ggcgacggt aacgacgtc gttaaagac ttgaaggtg 
      gaattgttg ggggaaatc gcgacgcct gaatgcatt 
      acgtacttg gataatgga gttttattt attggatcg 
      agattgggc gactcgcag ttggtgaaa ctgaccact 
      aaaccaaac gagagtggg tcgtacgtt accgtgatg 
      gagtctttt acgaatttg gcgccgatt ttggatatg 
      tgtgtcgtt gatttggag agacagggg cagggacag 
      ttggtgacg tgctcgggg gccttcaag gagggctcc 
      ttgaggatt ataaggaat ggtattggg attcaggag 
      catgcgtcg attgatttg ccggggatt aagggcatg 
      tgggcgctg caagtggca tcagatggc cgttacgac 
      aatacctta gtcctcgct ttcgtgggc caaactcgc 
      gtcttgagc ttgaacggt gaggaagtt gaagaaacg 
      gacattgct ggcttcgcc tccgaccag cagactttc 
      ttttgcggc aacgtcatc cacgaacaa atcgtacaa 
      attacccca atctctgcc cgtctaata tcagctcaa 
      aacaaaact ttgttggcg gaatggaaa ccgcctagc 
      gataaaaac atagcagta gtggcatgc aacacgtgc 
      caaatagtg gtttcgacc ggctctatt ttgtattat 
      attgaaatc caccaaaat gaactgatc atgaaaggc 
      gtgaccaca cttgacgtt gaggtggct tgtttggac 
      ataagccct ctgggggac ggtgtaaca acctccgaa 
      tatatcgct gtggggctc tggaccgat atcacagcc 
      cggattttg cgcataccc gacttgtct gaagccaca 
      aaggagtat ttaggggga gaaattata ccccgctcg 
      gttctaatg acctgtttc gagggtcat agctatttg 
      ttgtgtgcc ttgggtgac ggttcaatg ttctatttc 
      gtgtttcac aaagatacg ggaacgtta agtgacaaa 
      aaacgcgtc actttgggt acccaaccg actgtttta 
      cgtaccttc cgctcgttg agcactacg aatgttttt 
      gcttgttcg gatagaccg actgtaatt tattcatcc 
      aatcacaag ctcgttttt tctaacgtt aacatgaag 
      gaagttaat catatgtgt tcgcttaat gccgaggca 
      tatccggac agtttagct ttagcaaca gattcgtcc 
      gtcactatt gggacaatc gatgagata cagaagcta 
      catattagg acggtgcca ctgcaggag tctccaaag 
      agaatagcg taccaggag gagtcacag acttttggt 
      gttctgacg gcccgcatc gacatccaa gactccacc 
      ggcttgaac ccggctcgt ccgagcgct tcaacgatg 
      gctcagtcg acaacatct tcaagcagt gtcgggtct 
      ctctcaatg agtaaatca gggagcagc agtttgatc 
      ggtggtaac ataattccc gactacggc caagaagtc 
      gaagtccac aacttgctc attatcgat caacacacc 
      ttcgaagta ttgcatgcc caccaattg atgcagcaa 
      gaatatgca atgtcgata atctcaacc aatcgccta 
      ggtggcgac atgaacgaa tactacata gttggcact 
      gccacagtc aatccagaa gaatcggaa cccaagcaa 
      ggccgcatc ttaattttc cagtggaac gacaataaa 
      ctaacccaa gtgtctgag aaagaaatc aagggggct 
      tgctactcc cttgcggag ttcaacggc aaactcctg 
      gcgagtatc aacagcacg gtgaggctg ttcgagtgg 
      acggtggaa aaggagctg cgattggag tgctcccac 
      tttaataat attctaact ctgtttttg aaaaccaaa 
      ggcgatttt atcctcttg ggtgacttg atgcgcagc 
      atgaccttg ctgcagtac aaaacaatg gaaggtagc 
      tttgaagaa atcgcgagg gattataac ccgaactgg 
      atgacggct gttgaaata ctcgatgac gacattttc 
      ctaggggct gagaattcg tttaacata ttcgtttgt 
      cagaaagac agtgccgct acgacggac gaggaaagg 
      tcgcaaatg cacgaagtg gggcggttt cacgtcggg 
      gacatgatc aacgtcttc agacacgga tctctagtt 
      atgcaaaat ctgggagag acttcgacg ccgaccaca 
      ggctgtgtc ttgtttggc acagtcagt ggagccata 
      ggtttagtg acgcagata acccaagat ttttacgac 
      tttctccta gagctgcaa aataaacta tcgacggtt 
      attaaatcg gtcggtaaa attgatcac tcccaatgg 
      agagcgttc aacaccgat attaaaaca gagccgagt 
      gaaggcttc attgacggt gacttgatt gagagtttc 
      ctcgatttg tcgcacgac aagatgaag gaagtggcc 
      gatggatta cagataact ggggaaggt ggcatgaaa 
      caggattgt accgtcgat gatttagtc aagatggtc 
      gaagattta acgaggatt cactaattt gtcaggtgc 
      gttattatt ttatatata acaccagtg aagtgttag 
      gatcttaag t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY