Status
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Gene information
General information
TC033793
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010004,TC010005 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 10443252..10488117
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC033793
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04742

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033793 RA
      MCGPPFHRS GAPCTLRHV ANINKRHYA RGGRISCAL 
EGSACAPRR LRRPHSVSE SVSSMAVAR RLCCSQAAG 
GGPLLREPG PRSPDSLLD ITAKIVAEN IPFQRIEER 
YDRIPEPVQ RRIIFWSFP RNERDICMY SSLSRVPPV 
NAGAEPQNL SFCKGLKLL ETGCVDSVL QVGFHLSGI 
VTASRTSPL QTTLPCGVQ EPPQKFKVS VSFDRCKIT 
SVTCSCDTK DIFWCQHVV ALSLYRIRN AESVRLRVP 
ISVIHLKMR DNNEDFFDQ FNQKSISKV KRFNFKEII 
GKGSAKPTS DENAKIEVM STRVEAQKT INCEVPSKI 
NRPTSLNTS LLKTGSSDA FQVDTKDKI LKKFSFKKQ 
TVLKTTTSQ QSLESSSKQ DGCVGIFNK HVASSASSE 
SSKKFNFKK QVTLNTNCG TLNNVAEAR PTQNGDFLE 
TFKNHVTET SNKTTTSLE ILNTTQNEY VGTSPALGS 
HNSSQAGPS SNFSADDYM DDETFASFL ACEMDLETP 
QNNTKDVSP PPEATQSGV FTGRTDDSH EFRKKYPHT 
EVMYEVLHQ KFGLRHFRP HQEEIINAS LTQQDCFVL 
MPTGGGKSL CYQLPAVLM PGVTIVISP LRALISDQV 
DKLNALDIP SAHLCSDVK KADVDVIFQ KLHVREPIL 
KLLYLTPEK MSASGKVTD MIKSLYARN KLARFVIDE 
VHCLSQWGH DFRPDYKQL SNLRKQYPE VPIICLTAT 
ATKQVQGDV TNILGLKNP KTFIRSFNR PNIKYRVIP 
KNGIKVVEE ITKLIKQRF YRKSGIIYC LCRADCEKL 
AEDLCKLGI KAKAYHAGM SDSIREKQQ REWMQDQFH 
VIVATIAFG MGIDKPDVR FVIHNSMPK SVEAFYQES 
GRAGRDGEP SYSYLFYSY ADAGRLKRL MQMDRGVNK 
NALHGHYEN LYQMVSFCE NIVDCRRYL QLIHLGEKF 
DRKICMENK AMMCDNCEN IKNNKLSDV TKEARELGQ 
LVHDLARLE NVTLLYVAD VYKGSKLKK IVEKRHDQH 
RFYGGGSSL DRVDIQRIL INLVLRKIL TDFCTFNGD 
FPVVYIKPG VKFTTVNQP DLKIMLSIR ERIAPVAKV 
SMTKSSSDE SRSPTPSTS AAQPVAANK FKINAIKVQ 
CHEELLEEC RRLALERSV TLSSIMNLS AIKNMSEVL 
PNTKEDFMK IQHVTQANY EKFGEYFLK ITTKHREEL 
NKLQTPVAS FESTRSSNA FDDSDWWLD SSQSTSGVK 
RKSTSGYKK NAKRFKRNY YKKGKGKGK AKAKSTKKA 
PKNNKKGNS LGLMPLK
    CDS sequence
    • >TC033793 RA
      atgtgtggg cccccattc caccgttct ggcgccccc 
tgcaccttg cgccatgtg gcgaacata aacaaacga 
cattacgca cgcgggggt cgaatcagc tgtgccttg 
gaggggagc gcgtgcgcc cctcgccgc ctccgtcgg 
ccgcactca gtcagtgag agtgtgtcg tcgatggcg 
gtggccagg cggttgtgc tgctcccag gccgccggc 
ggggggccc ttgctgcgg gagccgggg ccgcgctcc 
ccggactcg ctgctcgac atcaccgcc aagatcgtc 
gccgagaac atccccttc cagcggatc gaggagcgc 
tacgaccgc atccccgag ccggtgcag cgcaggatc 
atcttctgg tcgttcccg aggaacgag cgtgacata 
tgcatgtac agttcgctg tcgcgagtg ccgcctgtt 
aacgcgggg gccgagccc cagaacctc tccttctgc 
aagggatta aagctgctg gagaccggg tgcgtcgac 
agcgtactg caagttggt tttcatcta agcggtatc 
gtaaccgcg tcccggact tcgcctctt cagacaacg 
ttgccttgt ggagttcaa gagccacct caaaaattc 
aaagtgtct gtgagtttt gacagatgc aaaattacc 
tctgtgacc tgcagttgc gacacgaag gatatcttc 
tggtgtcaa cacgtcgtg gcattgtcc ctctacaga 
attcggaat gcggagagc gtccggttg cgggttcca 
atttcagtg attcattta aaaatgcgc gataataac 
gaagatttt ttcgatcag tttaatcaa aagtcgatc 
tccaaagta aaaaggttc aatttcaag gaaattatc 
ggaaaagga tcggcaaaa ccaacatca gacgaaaat 
gcgaaaatt gaggttatg tctactaga gttgaagcc 
caaaaaaca attaactgt gaagtccca tcgaaaata 
aacagacca actagtcta aatactagc ttacttaaa 
actggatcg tctgatgcg tttcaagtt gacactaaa 
gacaaaatt cttaagaaa ttcagtttt aagaagcag 
acggtctta aagacaact acttcccaa cagtcgttg 
gaatcgagc tctaagcaa gacggctgt gttggcatt 
ttcaataaa cacgtcgct agtagtgca agtagtgaa 
tcttcgaaa aaatttaat tttaaaaag caagttacc 
ctgaacaca aattgtggg accttgaat aatgtggct 
gaggcaaga cctacacaa aacggagac ttccttgaa 
acgttcaaa aaccatgtc actgagacc tccaataag 
accaccaca tctctcgaa attttgaac accacacaa 
aatgaatat gttggaact tctccagct ttaggttca 
cataattcg tcccaagcg ggcccctca tctaatttt 
tctgccgac gattacatg gatgacgaa acctttgct 
agttttcta gcgtgtgag atggatttg gaaactcct 
caaaataat actaaggac gtaagtccc ccacctgag 
gccacgcag agtggcgtg tttaccggt cgcacagat 
gattcccat gaatttcgc aaaaagtac ccccataca 
gaagtcatg tatgaggtt ctccaccaa aaattcggg 
ttgaggcac tttcggccc caccaagaa gaaatcata 
aatgcgagt ttaacacag caggattgt ttcgttttg 
atgccgacc ggtgggggg aaaagtctt tgttaccaa 
ttacctgcc gttttgatg ccaggtgta acaatagta 
atttcgcca ttacgtgct ctaatcagc gaccaagtc 
gataaatta aatgctttg gatataccg tcggcgcat 
ttatgttca gacgttaag aaggccgat gttgatgta 
attttccaa aagcttcac gtccgggag ccaatcttg 
aagctgctt tatttgact ccggagaaa atgtcggct 
tcggggaaa gtaacagac atgattaag tcgttatac 
gcacgtaat aaattggcg aggtttgtc attgatgaa 
gttcattgt ttgtcgcaa tgggggcac gattttaga 
ccggactat aaacagttg tctaactta cgtaaacag 
taccctgaa gtaccgatc atttgtttg acggcaaca 
gccactaag caagtccaa ggcgatgtt acaaacatc 
cttggtctg aaaaaccct aaaacgttt atacggagt 
ttcaatcgg cccaatatc aagtataga gttattcca 
aaaaatggc attaaggtc gtagaggag ataacaaag 
ctgatcaaa cagaggttt taccgaaaa tcagggatt 
atctattgt ttgtgtcga gctgattgt gagaaacta 
gcagaggat ctgtgtaaa ttaggcatc aaagccaag 
gcatatcat gccggtatg agcgactct atccgcgaa 
aagcaacaa agggagtgg atgcaagac cagtttcat 
gtcatagtc gcaacgatt gcctttggt atggggatt 
gacaaaccg gatgttaga ttcgtcatt cataactcc 
atgccaaag tcagtagaa gcgttctat caagaatcg 
ggaagagca ggccgtgat ggtgaacct tcctactcc 
tatctcttc tactcgtac gctgacgct ggacgtttg 
aaacgtcta atgcagatg gatcgcggt gtcaataaa 
aacgcactt catggtcac tacgagaat ttataccaa 
atggtgtct ttctgcgaa aacatagtc gattgtaga 
cgctacttg caattgata catttaggc gaaaaattc 
gatcggaaa atatgcatg gagaacaaa gctatgatg 
tgcgacaac tgcgagaac attaaaaac aacaaacta 
tcagacgtt acgaaagaa gcacgagag ttgggtcag 
ttggtccac gatttagct cggcttgaa aatgtgact 
ttgctttac gtcgctgat gtgtataaa gggtcaaaa 
ttaaagaaa attgtagag aagagacat gaccagcat 
aggttttat ggcgggggg agttctctg gaccgtgtt 
gatatccaa aggattttg ataaactta gttttgcgg 
aaaatcttg accgacttt tgtacattt aacggggat 
tttccagtc gtctatata aaaccgggc gttaagttt 
accactgtt aaccaacca gatctaaaa ataatgctt 
tcgattaga gaaagaatt gctccggtt gcaaaagtg 
tcaatgacg aaaagctcg tctgacgag tcaagaagc 
ccaactccg tcgacttca gctgctcag ccagttgcc 
gctaacaaa tttaaaatc aacgctatt aaagtccag 
tgtcatgag gaactgctc gaggagtgt cgaagactg 
gcactggaa cgcagtgtg actctctcc tctattatg 
aacttgtca gctatcaaa aacatgtcg gaggttcta 
ccaaacacg aaggaagat tttatgaaa atccaacac 
gtgacacaa gcgaattac gaaaaattt ggagaatat 
ttccttaaa ataacaaca aaacacaga gaggagttg 
aataaattg cagacaccg gtagcttca tttgagagt 
actcgcagt tcaaatgca tttgatgat agcgactgg 
tggttggat agtagtcaa agtactagt ggagtaaaa 
cgaaaatca acgtcgggc tataagaaa aatgcgaaa 
cggtttaaa agaaattat tataagaaa ggaaaaggg 
aaaggaaag gctaaagcc aagagtacg aaaaaagcg 
ccgaaaaac aataaaaag gggaattcg ctaggctta 
atgcctttg aaataa
    mRNA sequence
    • >TC033793 RA
      ttggagcaa tttttttcg aggtcagtg tgcttagag 
cgcgtccct tcccatgtg tgggccccc attccaccg 
ttctggcgc cccctgcac cttgcgcca tgtggcgaa 
cataaacaa acgacatta cgcacgcgg gggtcgaat 
cagctgtgc cttggaggg gagcgcgtg cgcccctcg 
ccgcctccg tcggccgca ctcagtcag tgagagtgt 
gtcgtcgat ggcggtggc caggcggtt gtgctgctc 
ccaggccgc cggcggggg gcccttgct gcgggagcc 
ggggccgcg ctccccgga ctcgctgct cgacatcac 
cgccaagat cgtcgccga gaacatccc cttccagcg 
gatcgagga gcgctacga ccgcatccc cgagccggt 
gcagcgcag gatcatctt ctggtcgtt cccgaggaa 
cgagcgtga catatgcat gtacagttc gctgtcgcg 
agtgccgcc tgttaacgc gggggccga gccccagaa 
cctctcctt ctgcaaggg attaaagct gctggagac 
cgggtgcgt cgacagcgt actgcaagt tggttttca 
tctaagcgg tatcgtaac cgcgtcccg gacttcgcc 
tcttcagac aacgttgcc ttgtggagt tcaagagcc 
acctcaaaa attcaaagt gtctgtgag ttttgacag 
atgcaaaat tacctctgt gacctgcag ttgcgacac 
gaaggatat cttctggtg tcaacacgt cgtggcatt 
gtccctcta cagaattcg gaatgcgga gagcgtccg 
gttgcgggt tccaatttc agtgattca tttaaaaat 
gcgcgataa taacgaaga ttttttcga tcagtttaa 
tcaaaagtc gatctccaa agtaaaaag gttcaattt 
caaggaaat tatcggaaa aggatcggc aaaaccaac 
atcagacga aaatgcgaa aattgaggt tatgtctac 
tagagttga agcccaaaa aacaattaa ctgtgaagt 
cccatcgaa aataaacag accaactag tctaaatac 
tagcttact taaaactgg atcgtctga tgcgtttca 
agttgacac taaagacaa aattcttaa gaaattcag 
ttttaagaa gcagacggt cttaaagac aactacttc 
ccaacagtc gttggaatc gagctctaa gcaagacgg 
ctgtgttgg cattttcaa taaacacgt cgctagtag 
tgcaagtag tgaatcttc gaaaaaatt taattttaa 
aaagcaagt taccctgaa cacaaattg tgggacctt 
gaataatgt ggctgaggc aagacctac acaaaacgg 
agacttcct tgaaacgtt caaaaacca tgtcactga 
gacctccaa taagaccac cacatctct cgaaatttt 
gaacaccac acaaaatga atatgttgg aacttctcc 
agctttagg ttcacataa ttcgtccca agcgggccc 
ctcatctaa tttttctgc cgacgatta catggatga 
cgaaacctt tgctagttt tctagcgtg tgagatgga 
tttggaaac tcctcaaaa taatactaa ggacgtaag 
tcccccacc tgaggccac gcagagtgg cgtgtttac 
cggtcgcac agatgattc ccatgaatt tcgcaaaaa 
gtaccccca tacagaagt catgtatga ggttctcca 
ccaaaaatt cgggttgag gcactttcg gccccacca 
agaagaaat cataaatgc gagtttaac acagcagga 
ttgtttcgt tttgatgcc gaccggtgg ggggaaaag 
tctttgtta ccaattacc tgccgtttt gatgccagg 
tgtaacaat agtaatttc gccattacg tgctctaat 
cagcgacca agtcgataa attaaatgc tttggatat 
accgtcggc gcatttatg ttcagacgt taagaaggc 
cgatgttga tgtaatttt ccaaaagct tcacgtccg 
ggagccaat cttgaagct gctttattt gactccgga 
gaaaatgtc ggcttcggg gaaagtaac agacatgat 
taagtcgtt atacgcacg taataaatt ggcgaggtt 
tgtcattga tgaagttca ttgtttgtc gcaatgggg 
gcacgattt tagaccgga ctataaaca gttgtctaa 
cttacgtaa acagtaccc tgaagtacc gatcatttg 
tttgacggc aacagccac taagcaagt ccaaggcga 
tgttacaaa catccttgg tctgaaaaa ccctaaaac 
gtttatacg gagtttcaa tcggcccaa tatcaagta 
tagagttat tccaaaaaa tggcattaa ggtcgtaga 
ggagataac aaagctgat caaacagag gttttaccg 
aaaatcagg gattatcta ttgtttgtg tcgagctga 
ttgtgagaa actagcaga ggatctgtg taaattagg 
catcaaagc caaggcata tcatgccgg tatgagcga 
ctctatccg cgaaaagca acaaaggga gtggatgca 
agaccagtt tcatgtcat agtcgcaac gattgcctt 
tggtatggg gattgacaa accggatgt tagattcgt 
cattcataa ctccatgcc aaagtcagt agaagcgtt 
ctatcaaga atcgggaag agcaggccg tgatggtga 
accttccta ctcctatct cttctactc gtacgctga 
cgctggacg tttgaaacg tctaatgca gatggatcg 
cggtgtcaa taaaaacgc acttcatgg tcactacga 
gaatttata ccaaatggt gtctttctg cgaaaacat 
agtcgattg tagacgcta cttgcaatt gatacattt 
aggcgaaaa attcgatcg gaaaatatg catggagaa 
caaagctat gatgtgcga caactgcga gaacattaa 
aaacaacaa actatcaga cgttacgaa agaagcacg 
agagttggg tcagttggt ccacgattt agctcggct 
tgaaaatgt gactttgct ttacgtcgc tgatgtgta 
taaagggtc aaaattaaa gaaaattgt agagaagag 
acatgacca gcataggtt ttatggcgg ggggagttc 
tctggaccg tgttgatat ccaaaggat tttgataaa 
cttagtttt gcggaaaat cttgaccga cttttgtac 
atttaacgg ggattttcc agtcgtcta tataaaacc 
gggcgttaa gtttaccac tgttaacca accagatct 
aaaaataat gctttcgat tagagaaag aattgctcc 
ggttgcaaa agtgtcaat gacgaaaag ctcgtctga 
cgagtcaag aagcccaac tccgtcgac ttcagctgc 
tcagccagt tgccgctaa caaatttaa aatcaacgc 
tattaaagt ccagtgtca tgaggaact gctcgagga 
gtgtcgaag actggcact ggaacgcag tgtgactct 
ctcctctat tatgaactt gtcagctat caaaaacat 
gtcggaggt tctaccaaa cacgaagga agattttat 
gaaaatcca acacgtgac acaagcgaa ttacgaaaa 
atttggaga atatttcct taaaataac aacaaaaca 
cagagagga gttgaataa attgcagac accggtagc 
ttcatttga gagtactcg cagttcaaa tgcatttga 
tgatagcga ctggtggtt ggatagtag tcaaagtac 
tagtggagt aaaacgaaa atcaacgtc gggctataa 
gaaaaatgc gaaacggtt taaaagaaa ttattataa 
gaaaggaaa agggaaagg aaaggctaa agccaagag 
tacgaaaaa agcgccgaa aaacaataa aaaggggaa 
ttcgctagg cttaatgcc tttgaaata ataaatttg 
ttctgtggg aggtgtaca taattttca gttcgttgt 
taaggtcga aaatgggac aggatatgg ggtaatgaa 
ataattttt atagaaaaa t
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