Status
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Gene information
General information
TC033793
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010004,TC010005
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 10443252..10488117
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033793
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_04742

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033793 RA
      MCGPPFHRS GAPCTLRHV ANINKRHYA RGGRISCAL 
      EGSACAPRR LRRPHSVSE SVSSMAVAR RLCCSQAAG 
      GGPLLREPG PRSPDSLLD ITAKIVAEN IPFQRIEER 
      YDRIPEPVQ RRIIFWSFP RNERDICMY SSLSRVPPV 
      NAGAEPQNL SFCKGLKLL ETGCVDSVL QVGFHLSGI 
      VTASRTSPL QTTLPCGVQ EPPQKFKVS VSFDRCKIT 
      SVTCSCDTK DIFWCQHVV ALSLYRIRN AESVRLRVP 
      ISVIHLKMR DNNEDFFDQ FNQKSISKV KRFNFKEII 
      GKGSAKPTS DENAKIEVM STRVEAQKT INCEVPSKI 
      NRPTSLNTS LLKTGSSDA FQVDTKDKI LKKFSFKKQ 
      TVLKTTTSQ QSLESSSKQ DGCVGIFNK HVASSASSE 
      SSKKFNFKK QVTLNTNCG TLNNVAEAR PTQNGDFLE 
      TFKNHVTET SNKTTTSLE ILNTTQNEY VGTSPALGS 
      HNSSQAGPS SNFSADDYM DDETFASFL ACEMDLETP 
      QNNTKDVSP PPEATQSGV FTGRTDDSH EFRKKYPHT 
      EVMYEVLHQ KFGLRHFRP HQEEIINAS LTQQDCFVL 
      MPTGGGKSL CYQLPAVLM PGVTIVISP LRALISDQV 
      DKLNALDIP SAHLCSDVK KADVDVIFQ KLHVREPIL 
      KLLYLTPEK MSASGKVTD MIKSLYARN KLARFVIDE 
      VHCLSQWGH DFRPDYKQL SNLRKQYPE VPIICLTAT 
      ATKQVQGDV TNILGLKNP KTFIRSFNR PNIKYRVIP 
      KNGIKVVEE ITKLIKQRF YRKSGIIYC LCRADCEKL 
      AEDLCKLGI KAKAYHAGM SDSIREKQQ REWMQDQFH 
      VIVATIAFG MGIDKPDVR FVIHNSMPK SVEAFYQES 
      GRAGRDGEP SYSYLFYSY ADAGRLKRL MQMDRGVNK 
      NALHGHYEN LYQMVSFCE NIVDCRRYL QLIHLGEKF 
      DRKICMENK AMMCDNCEN IKNNKLSDV TKEARELGQ 
      LVHDLARLE NVTLLYVAD VYKGSKLKK IVEKRHDQH 
      RFYGGGSSL DRVDIQRIL INLVLRKIL TDFCTFNGD 
      FPVVYIKPG VKFTTVNQP DLKIMLSIR ERIAPVAKV 
      SMTKSSSDE SRSPTPSTS AAQPVAANK FKINAIKVQ 
      CHEELLEEC RRLALERSV TLSSIMNLS AIKNMSEVL 
      PNTKEDFMK IQHVTQANY EKFGEYFLK ITTKHREEL 
      NKLQTPVAS FESTRSSNA FDDSDWWLD SSQSTSGVK 
      RKSTSGYKK NAKRFKRNY YKKGKGKGK AKAKSTKKA 
      PKNNKKGNS LGLMPLK
    CDS sequence
    • >TC033793 RA
      atgtgtggg cccccattc caccgttct ggcgccccc 
      tgcaccttg cgccatgtg gcgaacata aacaaacga 
      cattacgca cgcgggggt cgaatcagc tgtgccttg 
      gaggggagc gcgtgcgcc cctcgccgc ctccgtcgg 
      ccgcactca gtcagtgag agtgtgtcg tcgatggcg 
      gtggccagg cggttgtgc tgctcccag gccgccggc 
      ggggggccc ttgctgcgg gagccgggg ccgcgctcc 
      ccggactcg ctgctcgac atcaccgcc aagatcgtc 
      gccgagaac atccccttc cagcggatc gaggagcgc 
      tacgaccgc atccccgag ccggtgcag cgcaggatc 
      atcttctgg tcgttcccg aggaacgag cgtgacata 
      tgcatgtac agttcgctg tcgcgagtg ccgcctgtt 
      aacgcgggg gccgagccc cagaacctc tccttctgc 
      aagggatta aagctgctg gagaccggg tgcgtcgac 
      agcgtactg caagttggt tttcatcta agcggtatc 
      gtaaccgcg tcccggact tcgcctctt cagacaacg 
      ttgccttgt ggagttcaa gagccacct caaaaattc 
      aaagtgtct gtgagtttt gacagatgc aaaattacc 
      tctgtgacc tgcagttgc gacacgaag gatatcttc 
      tggtgtcaa cacgtcgtg gcattgtcc ctctacaga 
      attcggaat gcggagagc gtccggttg cgggttcca 
      atttcagtg attcattta aaaatgcgc gataataac 
      gaagatttt ttcgatcag tttaatcaa aagtcgatc 
      tccaaagta aaaaggttc aatttcaag gaaattatc 
      ggaaaagga tcggcaaaa ccaacatca gacgaaaat 
      gcgaaaatt gaggttatg tctactaga gttgaagcc 
      caaaaaaca attaactgt gaagtccca tcgaaaata 
      aacagacca actagtcta aatactagc ttacttaaa 
      actggatcg tctgatgcg tttcaagtt gacactaaa 
      gacaaaatt cttaagaaa ttcagtttt aagaagcag 
      acggtctta aagacaact acttcccaa cagtcgttg 
      gaatcgagc tctaagcaa gacggctgt gttggcatt 
      ttcaataaa cacgtcgct agtagtgca agtagtgaa 
      tcttcgaaa aaatttaat tttaaaaag caagttacc 
      ctgaacaca aattgtggg accttgaat aatgtggct 
      gaggcaaga cctacacaa aacggagac ttccttgaa 
      acgttcaaa aaccatgtc actgagacc tccaataag 
      accaccaca tctctcgaa attttgaac accacacaa 
      aatgaatat gttggaact tctccagct ttaggttca 
      cataattcg tcccaagcg ggcccctca tctaatttt 
      tctgccgac gattacatg gatgacgaa acctttgct 
      agttttcta gcgtgtgag atggatttg gaaactcct 
      caaaataat actaaggac gtaagtccc ccacctgag 
      gccacgcag agtggcgtg tttaccggt cgcacagat 
      gattcccat gaatttcgc aaaaagtac ccccataca 
      gaagtcatg tatgaggtt ctccaccaa aaattcggg 
      ttgaggcac tttcggccc caccaagaa gaaatcata 
      aatgcgagt ttaacacag caggattgt ttcgttttg 
      atgccgacc ggtgggggg aaaagtctt tgttaccaa 
      ttacctgcc gttttgatg ccaggtgta acaatagta 
      atttcgcca ttacgtgct ctaatcagc gaccaagtc 
      gataaatta aatgctttg gatataccg tcggcgcat 
      ttatgttca gacgttaag aaggccgat gttgatgta 
      attttccaa aagcttcac gtccgggag ccaatcttg 
      aagctgctt tatttgact ccggagaaa atgtcggct 
      tcggggaaa gtaacagac atgattaag tcgttatac 
      gcacgtaat aaattggcg aggtttgtc attgatgaa 
      gttcattgt ttgtcgcaa tgggggcac gattttaga 
      ccggactat aaacagttg tctaactta cgtaaacag 
      taccctgaa gtaccgatc atttgtttg acggcaaca 
      gccactaag caagtccaa ggcgatgtt acaaacatc 
      cttggtctg aaaaaccct aaaacgttt atacggagt 
      ttcaatcgg cccaatatc aagtataga gttattcca 
      aaaaatggc attaaggtc gtagaggag ataacaaag 
      ctgatcaaa cagaggttt taccgaaaa tcagggatt 
      atctattgt ttgtgtcga gctgattgt gagaaacta 
      gcagaggat ctgtgtaaa ttaggcatc aaagccaag 
      gcatatcat gccggtatg agcgactct atccgcgaa 
      aagcaacaa agggagtgg atgcaagac cagtttcat 
      gtcatagtc gcaacgatt gcctttggt atggggatt 
      gacaaaccg gatgttaga ttcgtcatt cataactcc 
      atgccaaag tcagtagaa gcgttctat caagaatcg 
      ggaagagca ggccgtgat ggtgaacct tcctactcc 
      tatctcttc tactcgtac gctgacgct ggacgtttg 
      aaacgtcta atgcagatg gatcgcggt gtcaataaa 
      aacgcactt catggtcac tacgagaat ttataccaa 
      atggtgtct ttctgcgaa aacatagtc gattgtaga 
      cgctacttg caattgata catttaggc gaaaaattc 
      gatcggaaa atatgcatg gagaacaaa gctatgatg 
      tgcgacaac tgcgagaac attaaaaac aacaaacta 
      tcagacgtt acgaaagaa gcacgagag ttgggtcag 
      ttggtccac gatttagct cggcttgaa aatgtgact 
      ttgctttac gtcgctgat gtgtataaa gggtcaaaa 
      ttaaagaaa attgtagag aagagacat gaccagcat 
      aggttttat ggcgggggg agttctctg gaccgtgtt 
      gatatccaa aggattttg ataaactta gttttgcgg 
      aaaatcttg accgacttt tgtacattt aacggggat 
      tttccagtc gtctatata aaaccgggc gttaagttt 
      accactgtt aaccaacca gatctaaaa ataatgctt 
      tcgattaga gaaagaatt gctccggtt gcaaaagtg 
      tcaatgacg aaaagctcg tctgacgag tcaagaagc 
      ccaactccg tcgacttca gctgctcag ccagttgcc 
      gctaacaaa tttaaaatc aacgctatt aaagtccag 
      tgtcatgag gaactgctc gaggagtgt cgaagactg 
      gcactggaa cgcagtgtg actctctcc tctattatg 
      aacttgtca gctatcaaa aacatgtcg gaggttcta 
      ccaaacacg aaggaagat tttatgaaa atccaacac 
      gtgacacaa gcgaattac gaaaaattt ggagaatat 
      ttccttaaa ataacaaca aaacacaga gaggagttg 
      aataaattg cagacaccg gtagcttca tttgagagt 
      actcgcagt tcaaatgca tttgatgat agcgactgg 
      tggttggat agtagtcaa agtactagt ggagtaaaa 
      cgaaaatca acgtcgggc tataagaaa aatgcgaaa 
      cggtttaaa agaaattat tataagaaa ggaaaaggg 
      aaaggaaag gctaaagcc aagagtacg aaaaaagcg 
      ccgaaaaac aataaaaag gggaattcg ctaggctta 
      atgcctttg aaataa
    mRNA sequence
    • >TC033793 RA
      ttggagcaa tttttttcg aggtcagtg tgcttagag 
      cgcgtccct tcccatgtg tgggccccc attccaccg 
      ttctggcgc cccctgcac cttgcgcca tgtggcgaa 
      cataaacaa acgacatta cgcacgcgg gggtcgaat 
      cagctgtgc cttggaggg gagcgcgtg cgcccctcg 
      ccgcctccg tcggccgca ctcagtcag tgagagtgt 
      gtcgtcgat ggcggtggc caggcggtt gtgctgctc 
      ccaggccgc cggcggggg gcccttgct gcgggagcc 
      ggggccgcg ctccccgga ctcgctgct cgacatcac 
      cgccaagat cgtcgccga gaacatccc cttccagcg 
      gatcgagga gcgctacga ccgcatccc cgagccggt 
      gcagcgcag gatcatctt ctggtcgtt cccgaggaa 
      cgagcgtga catatgcat gtacagttc gctgtcgcg 
      agtgccgcc tgttaacgc gggggccga gccccagaa 
      cctctcctt ctgcaaggg attaaagct gctggagac 
      cgggtgcgt cgacagcgt actgcaagt tggttttca 
      tctaagcgg tatcgtaac cgcgtcccg gacttcgcc 
      tcttcagac aacgttgcc ttgtggagt tcaagagcc 
      acctcaaaa attcaaagt gtctgtgag ttttgacag 
      atgcaaaat tacctctgt gacctgcag ttgcgacac 
      gaaggatat cttctggtg tcaacacgt cgtggcatt 
      gtccctcta cagaattcg gaatgcgga gagcgtccg 
      gttgcgggt tccaatttc agtgattca tttaaaaat 
      gcgcgataa taacgaaga ttttttcga tcagtttaa 
      tcaaaagtc gatctccaa agtaaaaag gttcaattt 
      caaggaaat tatcggaaa aggatcggc aaaaccaac 
      atcagacga aaatgcgaa aattgaggt tatgtctac 
      tagagttga agcccaaaa aacaattaa ctgtgaagt 
      cccatcgaa aataaacag accaactag tctaaatac 
      tagcttact taaaactgg atcgtctga tgcgtttca 
      agttgacac taaagacaa aattcttaa gaaattcag 
      ttttaagaa gcagacggt cttaaagac aactacttc 
      ccaacagtc gttggaatc gagctctaa gcaagacgg 
      ctgtgttgg cattttcaa taaacacgt cgctagtag 
      tgcaagtag tgaatcttc gaaaaaatt taattttaa 
      aaagcaagt taccctgaa cacaaattg tgggacctt 
      gaataatgt ggctgaggc aagacctac acaaaacgg 
      agacttcct tgaaacgtt caaaaacca tgtcactga 
      gacctccaa taagaccac cacatctct cgaaatttt 
      gaacaccac acaaaatga atatgttgg aacttctcc 
      agctttagg ttcacataa ttcgtccca agcgggccc 
      ctcatctaa tttttctgc cgacgatta catggatga 
      cgaaacctt tgctagttt tctagcgtg tgagatgga 
      tttggaaac tcctcaaaa taatactaa ggacgtaag 
      tcccccacc tgaggccac gcagagtgg cgtgtttac 
      cggtcgcac agatgattc ccatgaatt tcgcaaaaa 
      gtaccccca tacagaagt catgtatga ggttctcca 
      ccaaaaatt cgggttgag gcactttcg gccccacca 
      agaagaaat cataaatgc gagtttaac acagcagga 
      ttgtttcgt tttgatgcc gaccggtgg ggggaaaag 
      tctttgtta ccaattacc tgccgtttt gatgccagg 
      tgtaacaat agtaatttc gccattacg tgctctaat 
      cagcgacca agtcgataa attaaatgc tttggatat 
      accgtcggc gcatttatg ttcagacgt taagaaggc 
      cgatgttga tgtaatttt ccaaaagct tcacgtccg 
      ggagccaat cttgaagct gctttattt gactccgga 
      gaaaatgtc ggcttcggg gaaagtaac agacatgat 
      taagtcgtt atacgcacg taataaatt ggcgaggtt 
      tgtcattga tgaagttca ttgtttgtc gcaatgggg 
      gcacgattt tagaccgga ctataaaca gttgtctaa 
      cttacgtaa acagtaccc tgaagtacc gatcatttg 
      tttgacggc aacagccac taagcaagt ccaaggcga 
      tgttacaaa catccttgg tctgaaaaa ccctaaaac 
      gtttatacg gagtttcaa tcggcccaa tatcaagta 
      tagagttat tccaaaaaa tggcattaa ggtcgtaga 
      ggagataac aaagctgat caaacagag gttttaccg 
      aaaatcagg gattatcta ttgtttgtg tcgagctga 
      ttgtgagaa actagcaga ggatctgtg taaattagg 
      catcaaagc caaggcata tcatgccgg tatgagcga 
      ctctatccg cgaaaagca acaaaggga gtggatgca 
      agaccagtt tcatgtcat agtcgcaac gattgcctt 
      tggtatggg gattgacaa accggatgt tagattcgt 
      cattcataa ctccatgcc aaagtcagt agaagcgtt 
      ctatcaaga atcgggaag agcaggccg tgatggtga 
      accttccta ctcctatct cttctactc gtacgctga 
      cgctggacg tttgaaacg tctaatgca gatggatcg 
      cggtgtcaa taaaaacgc acttcatgg tcactacga 
      gaatttata ccaaatggt gtctttctg cgaaaacat 
      agtcgattg tagacgcta cttgcaatt gatacattt 
      aggcgaaaa attcgatcg gaaaatatg catggagaa 
      caaagctat gatgtgcga caactgcga gaacattaa 
      aaacaacaa actatcaga cgttacgaa agaagcacg 
      agagttggg tcagttggt ccacgattt agctcggct 
      tgaaaatgt gactttgct ttacgtcgc tgatgtgta 
      taaagggtc aaaattaaa gaaaattgt agagaagag 
      acatgacca gcataggtt ttatggcgg ggggagttc 
      tctggaccg tgttgatat ccaaaggat tttgataaa 
      cttagtttt gcggaaaat cttgaccga cttttgtac 
      atttaacgg ggattttcc agtcgtcta tataaaacc 
      gggcgttaa gtttaccac tgttaacca accagatct 
      aaaaataat gctttcgat tagagaaag aattgctcc 
      ggttgcaaa agtgtcaat gacgaaaag ctcgtctga 
      cgagtcaag aagcccaac tccgtcgac ttcagctgc 
      tcagccagt tgccgctaa caaatttaa aatcaacgc 
      tattaaagt ccagtgtca tgaggaact gctcgagga 
      gtgtcgaag actggcact ggaacgcag tgtgactct 
      ctcctctat tatgaactt gtcagctat caaaaacat 
      gtcggaggt tctaccaaa cacgaagga agattttat 
      gaaaatcca acacgtgac acaagcgaa ttacgaaaa 
      atttggaga atatttcct taaaataac aacaaaaca 
      cagagagga gttgaataa attgcagac accggtagc 
      ttcatttga gagtactcg cagttcaaa tgcatttga 
      tgatagcga ctggtggtt ggatagtag tcaaagtac 
      tagtggagt aaaacgaaa atcaacgtc gggctataa 
      gaaaaatgc gaaacggtt taaaagaaa ttattataa 
      gaaaggaaa agggaaagg aaaggctaa agccaagag 
      tacgaaaaa agcgccgaa aaacaataa aaaggggaa 
      ttcgctagg cttaatgcc tttgaaata ataaatttg 
      ttctgtggg aggtgtaca taattttca gttcgttgt 
      taaggtcga aaatgggac aggatatgg ggtaatgaa 
      ataattttt atagaaaaa t
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