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Gene information
General information
TC009999 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 10535618..10538382
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009999
    iBeetle screen iB_04741

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009999 RA
      MESTSGPLS SAKATRSGR KVRKTNDLL FFSFNASAV 
      EKEDENIKP TSSKHNKQN LPRLEFDES GKSDKGSPF 
      LFKEVQKIS PPPVPKNKR KIVKAKRVL RSTKQCELE 
      DKCNDETLD LTTKSLKEK SSENSVNLV SDGEGSSRF 
      ITVTEKMAQ DGHVKKENK HGKTPKVKR QSGKGEFSS 
      FRDIEDFKS LEEPSENCK SVNLSPLNE DEGSSSYIE 
      VNEKSTQDG RITEKNSPS NGTFPNFVA SSKTPKTKK 
      QNLSSLEAD ESGKGKFSS FREIEDSKS FENCKSVNL 
      SPVNEDEGS SSYIEVQEK TTTEKISPT IDLPKFNFV 
      ASTAKKEKK SETSKDKKQ NVPVIELDE SGSSSEESF 
      SFKEIENSA KHCEENDET FNVETSNSY LDRKPIKQP 
      SENCKSQEN DSNVQSEQL ISKIGQTTV SEQLSEKKE 
      SFGSNLIVS LLPSPISPS TQSLKDEAP PYLSVHSAE 
      DPIQPSLNG SSDETSIRR RTYELENSV NDISLLKSV 
      SGKDGTKSS LGDDNSEEY VTPQVMFSK VTQRKRWNK 
      VNTTPFQIK GSPRWISSP RPSTSKSTP GVTKPRVSP 
      KLKNPKLSA KKPLSNVKV PNFAAIHKR AMDRVESIV 
      EYKQRKEER AKMLISGKK PQKQAEKPE KVGRKQLRF 
      APDTAEKDK SMTFIPLPS TPLPKGVKK NFDESLASE 
      NEEEIQPPV FKFPKPTFA FETKIRSPV FKFTKPTFT 
      SEERSALEK KINNLASHI PRMQKTNFG FLRREANDE 
      RRALVEKNC NINKFGISN PFHSKSAKA QVRLEEIKS 
      VATKSKPVK NTLEARKCT IKGVRSNRR FELLMNMRK 
      GK
    CDS sequence
    • >TC009999 RA
      atggaaagc accagtggc cccttaagc agtgccaaa 
      gcaacaaga agtggacga aaagtcaga aaaacgaac 
      gatttgctt ttcttcagt tttaatgct tctgcagtg 
      gaaaaagaa gacgaaaat attaagcca acgtcgtca 
      aagcataat aaacagaat ttgcctcgt ttggagttc 
      gatgagagc gggaaaagt gacaaagga agccctttt 
      ttgttcaag gaagttcaa aaaatctcg ccgccacct 
      gtaccaaaa aataaaaga aaaatcgtc aaagctaag 
      agagttctt aggagtacc aagcaatgt gaactggaa 
      gacaagtgc aatgatgag acattagat ctcactaca 
      aaaagtttg aaggagaaa tcttccgaa aattctgta 
      aatcttgtg agtgatggt gagggaagt tcacgtttc 
      atcacagtc acggagaaa atggctcaa gatggacac 
      gtgaaaaaa gaaaataaa catggcaag acaccaaag 
      gtcaaaaga cagagtggg aaaggagag ttttcttcg 
      tttagagac attgaagat tttaaatct cttgaagaa 
      ccgtcagaa aattgcaag agcgtaaat ttgagccct 
      ttaaatgaa gatgaggga agttcaagc tacattgaa 
      gtcaacgag aagagtaca caagatgga cgtattaca 
      gagaaaaat agtcctagc aatggtact ttccccaat 
      tttgttgct tcaagcaag acaccaaag accaaaaaa 
      cagaatttg tcttctttg gaagcggac gaaagtggg 
      aaaggaaag ttttcttcg tttagggaa attgaagat 
      tcaaaatct tttgaaaat tgcaagtct gtaaacttg 
      agtcctgtg aatgaagat gagggaagt tcaagctac 
      attgaagtg caggaaaag actactaca gagaagatc 
      agtcctacc attgatttg cccaaattt aattttgtt 
      gcttcaacg gcgaaaaaa gaaaaaaaa tctgaaaca 
      tcaaaggac aaaaaacaa aatgtgcct gtcattgag 
      ttggacgag agtgggagc agcagtgaa gagtccttt 
      tcatttaaa gaaattgaa aattccgcc aagcattgt 
      gaggaaaat gatgagaca tttaatgtt gaaacaagt 
      aattcatat ttggatcga aaaccaatt aaacaaccg 
      tctgaaaat tgcaagagt caagaaaac gacagtaat 
      gtgcaaagt gaacagttg atttcaaag attggccaa 
      accactgtc tctgagcaa ttaagtgaa aagaaagag 
      tcttttggt tcaaacttg attgtcagt ttattgcca 
      agccccata tcaccttca acacaaagt ttaaaagat 
      gaagctcca ccgtatttg tctgtgcat tcagctgaa 
      gacccaatc cagccgtct ttgaatgga agcagtgat 
      gagacctct atcagaagg agaacgtac gaactggaa 
      aatagtgtt aatgatata tcactcttg aagtccgta 
      tcagggaaa gatggaacc aaatcgtcc cttggtgac 
      gataattca gaagagtat gtaacgccc caagtgatg 
      ttttctaaa gttactcaa agaaaaagg tggaataaa 
      gttaacacc acaccgttt caaattaag ggcagtccg 
      aggtggatt agcagtcca agaccgtca acctccaag 
      tcgacacca ggagttaca aagccacga gtttcgcca 
      aaattaaaa aatcccaaa ctctcagcg aaaaaaccc 
      ctttctaat gtcaaagtg ccaaacttt gcggcaatt 
      cacaaacga gcaatggat agggttgaa agtatagtt 
      gaatacaaa cagagaaag gaggaaaga gcaaagatg 
      ttaatatca gggaaaaag ccgcaaaaa caagcggaa 
      aaacccgag aaagtaggg agaaaacag ttgaggttt 
      gccccagac actgctgag aaggataaa tcgatgacg 
      tttattccc ttgccctca acaccattg ccaaagggt 
      gtgaagaag aactttgat gaaagtctt gcgagtgag 
      aacgaagag gaaatccaa cctcctgtc ttcaaattt 
      ccgaaacca actttcgcg ttcgagact aaaatacgc 
      tcgcctgtt ttcaagttc accaaaccg acttttaca 
      agtgaagag agaagtgca ctggaaaag aaaattaat 
      aaccttgca agtcacatc ccacgaatg cagaaaacc 
      aatttcgga ttccttagg cgtgaagca aacgatgaa 
      agaagagcc ctggtggag aagaattgc aatattaat 
      aaatttgga atttcaaac ccattccac tccaaaagt 
      gcaaaagca caagtcaga cttgaagaa atcaaaagt 
      gtcgccact aaaagcaag ccagttaaa aacacactt 
      gaggctcga aaatgcaca atcaagggg gtgaggtcg 
      aaccgaaga tttgaatta ttgatgaac atgaggaag 
      gggaagtag
    mRNA sequence
    • >TC009999 RA
      tgtcagatt caaaaaaac ggttttgtt gtcggtttg 
      tcatttgtg tgccttgtt tactgtaaa gttactata 
      aaaagcgaa taattggat ttaacacaa cgttaaagc 
      ttgcattaa aatgtcagt gaggtccag cagacgttt 
      aaataaaaa actaaatcc acctgaaat ggaaagcac 
      cagtggccc cttaagcag tgccaaagc aacaagaag 
      tggacgaaa agtcagaaa aacgaacga tttgctttt 
      cttcagttt taatgcttc tgcagtgga aaaagaaga 
      cgaaaatat taagccaac gtcgtcaaa gcataataa 
      acagaattt gcctcgttt ggagttcga tgagagcgg 
      gaaaagtga caaaggaag ccctttttt gttcaagga 
      agttcaaaa aatctcgcc gccacctgt accaaaaaa 
      taaaagaaa aatcgtcaa agctaagag agttcttag 
      gagtaccaa gcaatgtga actggaaga caagtgcaa 
      tgatgagac attagatct cactacaaa aagtttgaa 
      ggagaaatc ttccgaaaa ttctgtaaa tcttgtgag 
      tgatggtga gggaagttc acgtttcat cacagtcac 
      ggagaaaat ggctcaaga tggacacgt gaaaaaaga 
      aaataaaca tggcaagac accaaaggt caaaagaca 
      gagtgggaa aggagagtt ttcttcgtt tagagacat 
      tgaagattt taaatctct tgaagaacc gtcagaaaa 
      ttgcaagag cgtaaattt gagcccttt aaatgaaga 
      tgagggaag ttcaagcta cattgaagt caacgagaa 
      gagtacaca agatggacg tattacaga gaaaaatag 
      tcctagcaa tggtacttt ccccaattt tgttgcttc 
      aagcaagac accaaagac caaaaaaca gaatttgtc 
      ttctttgga agcggacga aagtgggaa aggaaagtt 
      ttcttcgtt tagggaaat tgaagattc aaaatcttt 
      tgaaaattg caagtctgt aaacttgag tcctgtgaa 
      tgaagatga gggaagttc aagctacat tgaagtgca 
      ggaaaagac tactacaga gaagatcag tcctaccat 
      tgatttgcc caaatttaa ttttgttgc ttcaacggc 
      gaaaaaaga aaaaaaatc tgaaacatc aaaggacaa 
      aaaacaaaa tgtgcctgt cattgagtt ggacgagag 
      tgggagcag cagtgaaga gtccttttc atttaaaga 
      aattgaaaa ttccgccaa gcattgtga ggaaaatga 
      tgagacatt taatgttga aacaagtaa ttcatattt 
      ggatcgaaa accaattaa acaaccgtc tgaaaattg 
      caagagtca agaaaacga cagtaatgt gcaaagtga 
      acagttgat ttcaaagat tggccaaac cactgtctc 
      tgagcaatt aagtgaaaa gaaagagtc ttttggttc 
      aaacttgat tgtcagttt attgccaag ccccatatc 
      accttcaac acaaagttt aaaagatga agctccacc 
      gtatttgtc tgtgcattc agctgaaga cccaatcca 
      gccgtcttt gaatggaag cagtgatga gacctctat 
      cagaaggag aacgtacga actggaaaa tagtgttaa 
      tgatatatc actcttgaa gtccgtatc agggaaaga 
      tggaaccaa atcgtccct tggtgacga taattcaga 
      agagtatgt aacgcccca agtgatgtt ttctaaagt 
      tactcaaag aaaaaggtg gaataaagt taacaccac 
      accgtttca aattaaggg cagtccgag gtggattag 
      cagtccaag accgtcaac ctccaagtc gacaccagg 
      agttacaaa gccacgagt ttcgccaaa attaaaaaa 
      tcccaaact ctcagcgaa aaaacccct ttctaatgt 
      caaagtgcc aaactttgc ggcaattca caaacgagc 
      aatggatag ggttgaaag tatagttga atacaaaca 
      gagaaagga ggaaagagc aaagatgtt aatatcagg 
      gaaaaagcc gcaaaaaca agcggaaaa acccgagaa 
      agtagggag aaaacagtt gaggtttgc cccagacac 
      tgctgagaa ggataaatc gatgacgtt tattccctt 
      gccctcaac accattgcc aaagggtgt gaagaagaa 
      ctttgatga aagtcttgc gagtgagaa cgaagagga 
      aatccaacc tcctgtctt caaatttcc gaaaccaac 
      tttcgcgtt cgagactaa aatacgctc gcctgtttt 
      caagttcac caaaccgac ttttacaag tgaagagag 
      aagtgcact ggaaaagaa aattaataa ccttgcaag 
      tcacatccc acgaatgca gaaaaccaa tttcggatt 
      ccttaggcg tgaagcaaa cgatgaaag aagagccct 
      ggtggagaa gaattgcaa tattaataa atttggaat 
      ttcaaaccc attccactc caaaagtgc aaaagcaca 
      agtcagact tgaagaaat caaaagtgt cgccactaa 
      aagcaagcc agttaaaaa cacacttga ggctcgaaa 
      atgcacaat caagggggt gaggtcgaa ccgaagatt 
      tgaattatt gatgaacat gaggaaggg gaagtaggt 
      ttagttttt gttttacat tttaacgtt tgtactcat 
      tacacattt aataa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY