Status
Please wait ...
Gene information
General information
TC009998 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 10542148..10543572
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009998
    iBeetle screen iB_04740

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 8 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009998 RA
      MAVAKPSNR NAAFEDLSN VEFETSEDV EVIEQFKNM 
      HLKEELLRG VFAYGFEKP SAIQQRAIK PIIKGRDVI 
      AQAQSGTGK TATFSIAIL QSLDLQTRE TQVLCLSPT 
      RELAVQIQK VILALGDFM NVQCHACIG GTNLGEDIR 
      KLDYGQHVV SGTPGRVYD MIRRRALRT RSVKMLVLD 
      EADEMLNKG FKEQIYDVY RFLPPSTQV VLISATLPH 
      EILEITSKF MTDPIRILV KRDELTLEG IKQFFVAVE 
      REEWKFDTL CDLYDTLTI TQAVIFCNT KRKVDWLTE 
      KMRENNFTV SSMHGDMPQ KERDNIMKE FRSGQSRVL 
      ITTDVWARG IDVQQVSLV INYDLPNNR ELYIHRIGR 
      SGRFGRKGV AINFVKSDD IRILRDIEQ YYSTQIDEM 
      PMNVADLI
    CDS sequence
    • >TC009998 RA
      atggctgtc gcaaaacct tcaaaccgc aatgcggct 
      ttcgaagac ttgtcgaac gttgagttc gaaaccagc 
      gaagatgtg gaagtcatc gagcaattt aagaatatg 
      cacctgaag gaggagctc cttcgcggt gtctttgca 
      tatggtttt gagaaacca tcagctatc caacagagg 
      gctatcaag ccgataata aagggccga gatgttatc 
      gctcaagcc cagtcaggt accggcaaa acggccaca 
      ttctcgatc gctattttg cagtcgctt gacttgcaa 
      acgcgggaa acccaagta ctttgcttg tcgccaacg 
      agggagttg gccgtgcaa atccagaaa gttatcctg 
      gctttgggg gactttatg aacgtgcag tgtcacgcc 
      tgcattggt gggaccaat ctgggagag gatatcagg 
      aagctggat tatgggcaa catgttgtt agtggaaca 
      cctggaaga gtttacgac atgatcaga cggcgcgcc 
      cttaggacc aggtccgtc aaaatgctg gttttggac 
      gaagccgac gaaatgtta aacaaaggc ttcaaggag 
      caaatctac gacgtttac aggttcctg ccgccctcc 
      actcaagtc gtgttgatt tctgcgact ttgccccac 
      gagattttg gagatcacg tcgaagttc atgacggac 
      ccgatccgt attttggta aaacgtgat gaattaact 
      cttgaaggc atcaagcaa tttttcgtc gcggttgag 
      cgtgaggag tggaaattt gacacactt tgtgacttg 
      tacgatact ttaaccatc acgcaagct gttatcttt 
      tgcaacact aaacgcaaa gtcgattgg ttgactgaa 
      aaaatgcgc gaaaacaat ttcaccgtg agttccatg 
      cacggtgat atgccccag aaggagaga gataatatt 
      atgaaggag ttcaggtcg ggacagagt cgtgtactt 
      atcacgacc gatgtgtgg gcgagaggt atcgacgtc 
      cagcaagtc agtttggtc attaattac gatttgccg 
      aacaaccgt gagttgtac attcaccgt attgggcga 
      tctggcagg tttggtaga aagggtgtt gcgattaat 
      ttcgtcaag tcggacgat atcaggatt ttgcgggat 
      attgagcag tattattcc acacagatt gacgaaatg 
      ccgatgaat gtcgctgat ttaatttaa
    mRNA sequence
    • >TC009998 RA
      ttcaattaa atcatctaa actttactt taacacacg 
      ttctaattt ctgacggag gcaatggct gtcgcaaaa 
      ccttcaaac cgcaatgcg gctttcgaa gacttgtcg 
      aacgttgag ttcgaaacc agcgaagat gtggaagtc 
      atcgagcaa tttaagaat atgcacctg aaggaggag 
      ctccttcgc ggtgtcttt gcatatggt tttgagaaa 
      ccatcagct atccaacag agggctatc aagccgata 
      ataaagggc cgagatgtt atcgctcaa gcccagtca 
      ggtaccggc aaaacggcc acattctcg atcgctatt 
      ttgcagtcg cttgacttg caaacgcgg gaaacccaa 
      gtactttgc ttgtcgcca acgagggag ttggccgtg 
      caaatccag aaagttatc ctggctttg ggggacttt 
      atgaacgtg cagtgtcac gcctgcatt ggtgggacc 
      aatctggga gaggatatc aggaagctg gattatggg 
      caacatgtt gttagtgga acacctgga agagtttac 
      gacatgatc agacggcgc gcccttagg accaggtcc 
      gtcaaaatg ctggttttg gacgaagcc gacgaaatg 
      ttaaacaaa ggcttcaag gagcaaatc tacgacgtt 
      tacaggttc ctgccgccc tccactcaa gtcgtgttg 
      atttctgcg actttgccc cacgagatt ttggagatc 
      acgtcgaag ttcatgacg gacccgatc cgtattttg 
      gtaaaacgt gatgaatta actcttgaa ggcatcaag 
      caatttttc gtcgcggtt gagcgtgag gagtggaaa 
      tttgacaca ctttgtgac ttgtacgat actttaacc 
      atcacgcaa gctgttatc ttttgcaac actaaacgc 
      aaagtcgat tggttgact gaaaaaatg cgcgaaaac 
      aatttcacc gtgagttcc atgcacggt gatatgccc 
      cagaaggag agagataat attatgaag gagttcagg 
      tcgggacag agtcgtgta cttatcacg accgatgtg 
      tgggcgaga ggtatcgac gtccagcaa gtcagtttg 
      gtcattaat tacgatttg ccgaacaac cgtgagttg 
      tacattcac cgtattggg cgatctggc aggtttggt 
      agaaagggt gttgcgatt aatttcgtc aagtcggac 
      gatatcagg attttgcgg gatattgag cagtattat 
      tccacacag attgacgaa atgccgatg aatgtcgct 
      gatttaatt taaacgaat aacattttt tatgtcctt 
      atattatgt aagaagttt gtgta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY