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Gene information
General information
TC009974 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 10842988..10849775
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009974
    iBeetle screen iB_04736

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009974 RA
      MGSTSSKFK KYLQHGDEF AAMQIYQSS PELRKNLDP 
      NLSYGENHN HNTALHYAA KHGMKHLLR TFLNDLGGN 
      PNKKNGLNE SVLHSACQL NQTKSFSAQ ERRAACVQL 
      LLYWKGGPL ANGGRERVD LSSQDKEGN TALHWAAST 
      GLKRCVELL LAHGAPLFI ENNDKLTPC DLAMRGSHH 
      DIARLLESR MVFADNADL VNEDEIITE QEEVYSGLR 
      SQDLQEAKD QLLVDTSDM LKIPLFTAE ALLRNNEWS 
      RELLLEKWM KDPVECCQI AGVQAPSSV LKHAGSLES 
      SISAETNDE NEIMCEICL STISNWEQP VKMSCKHSF 
      CKTCWESYL TTKIQDGDA HHILCPAYQ CHILVPVEL 
      IEKLVSPDM ARRYLQFDI KAFVESNKS IKWCPIAGC 
      GRAVRLPEA EQTGNRVNN KSAPITSHA VDCGNAHFF 
      CWECLGEAH APCGCKQWQ EWQIKIAEI KPEELKASC 
      SGSEDAANC LWLVTNSKP CPNCKSPIQ KNEGCNHMK 
      CSKCKFDFC WVCQESWKR HSSATGGYF RCNRFEAVH 
      KADEKQGNL ISEALDRNN QMQEMSRFL HFYTRFRNH 
      ENSQKLEEP LLTSVRQKR EVLASSLGL KSTEDFGEK 
      GTKFIEDGV RELLKARRV LCGSYVYGY YLEDDGYNK 
      TIFEFMQNE LEEVTEKLS EMIARPYLR TPKAVIVQT 
      TALARRKRH EFVRAVAQG LIPPETPPS QRKRKRKYL 
      DTDLTQTSA WLRDCEWPE YDSDEDNEV SFKQFSGGS 
      PNSKEHQCG FLCSRHGCG RSDYNMELI IALEMSRLQ 
      MIEDRMKQA QANAITPDP SSSISNNSN ESADDQLKL 
      AIQLSLQES GAAEETVQS QPYQKSSAD WTVDYFLKS 
      LANHKIDLK SLDVNKLGT ETDRELFFR PCNGNEDGR 
      FQISDIHEK GFAFEDVDD YDDADVGLK RSHSTGDLC 
      VRRSGRGTR VRMNGDEPR YHLDSDHSS QHDDKPEDL 
      ARKILALPS TSVRTKQFL LLHHTYPEE ESYQGQSSI 
      EDTTTSDSI NADMEVCGI LSSVTDEDI GKSYNEEEE 
      IKKTEMTKI VECKKQTHN PFASLKAKL CSAHLPKTS 
      YLTRIAVNK SIGLLSSDH KVKKNCDIL KDDRCKSEK 
      HSPKSIKHQ KCCRGGSFS KSTGFLLED QRKSCNLRI 
      KICNSPDSK CRKGHTLET DSSNEYESE TGIPKSPTL 
      FISGVSISR TPEPKSPGM ILIAGGRQS RSSSLSVPV 
      PLTSCSLNI SSTSLNNTL PPEPFTPSL VRSTTPANS 
      RSNIPSPSN SSPKKEANA KSKSASEPE REREMFRFP 
      KSSTDGALS SVLHIQESN LSSDDFHEA LFLLERSPK 
      TRDSKRRKK SKKNKEKDD KKEDVSSVL
    CDS sequence
    • >TC009974 RA
      atgggaagc acttcatcc aaattcaaa aaatatctc 
      caacacggc gatgagttt gcagcgatg caaatttac 
      caaagttcc ccggaactc cgcaaaaac ctggaccct 
      aatttaagt tacggcgaa aaccacaac cacaacact 
      gctctacat tacgctgcc aaacacggt atgaagcat 
      ctactgagg acgtttcta aacgatttg ggaggcaat 
      ccgaacaag aaaaacggc ttaaacgaa tcggttcta 
      cacagcgct tgccagctc aaccaaacc aaaagtttc 
      agcgcacaa gagagaaga gctgcatgt gtccaactg 
      ctcctctat tggaaagga gggccgcta gccaacgga 
      ggccgagaa agggttgat ttaagctcg caagataag 
      gagggcaat acggccctg cactgggcc gcctccacc 
      ggcctcaag cgctgtgtc gaactctta ctagcacat 
      ggagctccc ctttttatc gaaaacaac gataaatta 
      actccgtgc gatttagca atgaggggg tcccatcac 
      gacattgct agactgcta gaaagccgg atggttttt 
      gctgataat gctgatttg gtgaacgaa gacgagata 
      attacagaa caagaggaa gtttacagt ggacttaga 
      agtcaagat ctacaagaa gctaaagac cagttgctt 
      gttgatacg tccgatatg cttaaaatc ccattattt 
      acagccgag gctcttttg cgaaataat gaatggtcc 
      cgtgagctt cttcttgaa aagtggatg aaagatccg 
      gttgaatgt tgccaaatc gctggtgtt caagctcct 
      tcttccgtt ctcaaacat gcaggttcc ttagaatcc 
      agtatttca gcagaaact aacgacgag aatgaaata 
      atgtgtgag atttgtttg tcgacaata tcaaactgg 
      gagcaacca gttaaaatg tcctgtaag cactccttt 
      tgtaaaact tgctgggaa agctacctc acaaccaaa 
      attcaggat ggcgatgcc catcacatc ctctgtcca 
      gcttatcag tgtcacatc ttagttccc gttgaactt 
      atcgagaag ttagtaagt cccgacatg gcgcggaga 
      tatctccag ttcgacata aaggccttt gtcgagagc 
      aataaaagc atcaaatgg tgtcccatc gccggttgc 
      ggccgagcg gtccggtta ccggaagcg gaacaaacc 
      ggaaaccgg gttaacaat aaatcagct ccgataacc 
      tcgcacgcg gtggactgc ggaaacgcg cattttttc 
      tgttgggag tgcttgggc gaagcacat gcaccttgc 
      ggatgtaaa caatggcaa gagtggcag atcaaaatc 
      gccgaaatt aaaccggaa gagcttaaa gcttcgtgc 
      agcggaagc gaagatgcc gccaactgc ctctggctt 
      gtcaccaac tcgaaaccc tgtccaaac tgcaaaagt 
      ccaatccaa aaaaacgaa ggctgtaac cacatgaag 
      tgttccaaa tgcaaattc gacttttgt tgggtgtgt 
      caagaatcg tggaagaga cacagttcc gcgaccggg 
      ggctatttc cgctgtaac cgcttcgag gccgttcat 
      aaggccgac gaaaagcaa ggaaattta atttccgaa 
      gcgctagac agaaataat caaatgcaa gaaatgtct 
      cgtttttta catttttat acgcgcttt agaaaccac 
      gaaaacagc cagaagctc gaagaaccg ctcctaacc 
      agcgttcgg caaaaaagg gaagtgctc gccagcagt 
      ctcggatta aaaagcacc gaagatttc ggggagaaa 
      ggaactaaa ttcatcgaa gacggtgtg cgggaatta 
      ttaaaagcc cggagagtt ttatgcggt tcttacgtt 
      tacggttat tacctggaa gacgacggc tataacaaa 
      accatcttt gaatttatg cagaacgag ttggaggaa 
      gtgaccgaa aagttgagc gaaatgata gcgcgcccc 
      tacttgcgc acaccgaaa gccgtgatt gtgcaaacc 
      acagccctg gcgaggcga aaaaggcac gaattcgtg 
      agggcagtg gcgcaaggg ctgatcccg ccggaaacg 
      ccgccctct caaagaaaa cgcaagcga aaatatctc 
      gacactgat ttaacgcaa acaagcgcc tggttgcgt 
      gactgtgaa tggcctgaa tacgattca gacgaggat 
      aatgaagta agtttcaaa caattttct ggaggctct 
      ccaaactcc aaagagcat caatgcggc tttttgtgc 
      agcagacac ggatgtggc cggtctgat tacaacatg 
      gagctgata atagctctc gagatgtct agacttcag 
      atgattgaa gataggatg aaacaagca caagccaat 
      gcaatcacg cccgatccc agctcgagt atttcgaat 
      aatagcaac gaaagtgcc gatgatcag ctcaaattg 
      gctattcag ctcagtttg caggagtcg ggggcggcg 
      gaagagacg gttcagagc caaccgtat caaaagagc 
      agtgcggac tggacggtt gattacttc ctcaagtct 
      ttagcgaac cacaaaatt gatctgaag agtctcgat 
      gttaacaaa cttggcacg gagactgac agggagttg 
      ttctttcgg ccttgtaac gggaatgaa gatggaagg 
      ttccaaatt tctgatatt cacgaaaaa ggttttgcg 
      tttgaagat gttgatgat tatgatgat gcggacgtt 
      ggtctgaaa cgctcacac tcgactgga gatctctgt 
      gtccgaaga agtgggaga ggaacaagg gttcggatg 
      aatggagat gaacctcgt taccacttg gattcagac 
      catagcagt cagcatgat gataaaccg gaagatctg 
      gcccggaaa attctagct ttgccttcc acctccgtg 
      cgtactaaa caatttctt ttgcttcat catacttac 
      ccagaggaa gagtcgtat caagggcaa agctcgatc 
      gaagatact acgacttcg gattcgata aacgccgac 
      atggaagtt tgtggtatt ttgagttcg gtgaccgat 
      gaagacatt ggtaaaagt tacaacgag gaagaagaa 
      ataaagaag actgaaatg acaaaaata gtcgaatgt 
      aagaaacaa acccacaat cctttcgcc tctctgaag 
      gcgaaattg tgttcagca cacctgcct aaaactagt 
      tacttgacg cggattgca gtgaacaaa agcatagga 
      cttctcagt agtgatcat aaagtaaaa aagaactgt 
      gatatcctc aaagatgac cgatgcaag agcgaaaag 
      cacagcccc aaaagcata aaacatcag aagtgttgc 
      cgcggcggt agtttctcc aagtccacg ggattcctg 
      ttggaagac cagcggaaa agttgcaac ttgcggata 
      aaaatctgc aacagtccc gactccaag tgccgcaaa 
      ggccacact ctggagacg gacagttcg aacgagtac 
      gaatccgag actggcatc cccaagtca cccacgttg 
      ttcatatcg ggagtgtcc atctcccga acgccagaa 
      ccaaaatca cccgggatg atcctaata gcgggtggt 
      agacaatcg aggtcgtct agtttaagt gtacctgtg 
      cctctaact tcgtgttct ttaaacatt tctagtact 
      agtcttaac aatactcta ccaccggaa ccgttcacg 
      ccatctctg gtccgttcg acaacccca gccaattca 
      cgctcgaat atccccagt ccctcgaac tcgagtccc 
      aaaaaagaa gccaatgcc aaatcgaag agcgccagc 
      gagccggaa cgcgagagg gaaatgttc aggtttccc 
      aagtcttcg acggacggt gctttgagc tcggtcttg 
      cacattcaa gaatcgaat ttaagttcg gacgacttc 
      cacgaagcg ctattcctg ctggaacgg tctcccaaa 
      acgagagac tcaaaacgg cgaaagaaa tccaagaag 
      aataaagag aaagatgat aaaaaggag gacgttagc 
      tccgtattg taa
    mRNA sequence
    • >TC009974 RA
      ttctgatac acactattt ggtcgctat catgaaaat 
      gtcaattcc ttgtcacaa atatcgcaa aatagataa 
      agaaaatgt cagatagta actcgccct gttactggc 
      tgagaaaat gggcatttt cgacacgaa gctgcgttt 
      cttgacaag aaaccgacc gctaacaag tacacatat 
      cgaattttt ttatacaac cgtttgaaa ttagtttag 
      aactccctc aacataatg ggaagcact tcatccaaa 
      ttcaaaaaa tatctccaa cacggcgat gagtttgca 
      gcgatgcaa atttaccaa agttccccg gaactccgc 
      aaaaacctg gaccctaat ttaagttac ggcgaaaac 
      cacaaccac aacactgct ctacattac gctgccaaa 
      cacggtatg aagcatcta ctgaggacg tttctaaac 
      gatttggga ggcaatccg aacaagaaa aacggctta 
      aacgaatcg gttctacac agcgcttgc cagctcaac 
      caaaccaaa agtttcagc gcacaagag agaagagct 
      gcatgtgtc caactgctc ctctattgg aaaggaggg 
      ccgctagcc aacggaggc cgagaaagg gttgattta 
      agctcgcaa gataaggag ggcaatacg gccctgcac 
      tgggccgcc tccaccggc ctcaagcgc tgtgtcgaa 
      ctcttacta gcacatgga gctcccctt tttatcgaa 
      aacaacgat aaattaact ccgtgcgat ttagcaatg 
      agggggtcc catcacgac attgctaga ctgctagaa 
      agccggatg gtttttgct gataatgct gatttggtg 
      aacgaagac gagataatt acagaacaa gaggaagtt 
      tacagtgga cttagaagt caagatcta caagaagct 
      aaagaccag ttgcttgtt gatacgtcc gatatgctt 
      aaaatccca ttatttaca gccgaggct cttttgcga 
      aataatgaa tggtcccgt gagcttctt cttgaaaag 
      tggatgaaa gatccggtt gaatgttgc caaatcgct 
      ggtgttcaa gctccttct tccgttctc aaacatgca 
      ggttcctta gaatccagt atttcagca gaaactaac 
      gacgagaat gaaataatg tgtgagatt tgtttgtcg 
      acaatatca aactgggag caaccagtt aaaatgtcc 
      tgtaagcac tccttttgt aaaacttgc tgggaaagc 
      tacctcaca accaaaatt caggatggc gatgcccat 
      cacatcctc tgtccagct tatcagtgt cacatctta 
      gttcccgtt gaacttatc gagaagtta gtaagtccc 
      gacatggcg cggagatat ctccagttc gacataaag 
      gcctttgtc gagagcaat aaaagcatc aaatggtgt 
      cccatcgcc ggttgcggc cgagcggtc cggttaccg 
      gaagcggaa caaaccgga aaccgggtt aacaataaa 
      tcagctccg ataacctcg cacgcggtg gactgcgga 
      aacgcgcat tttttctgt tgggagtgc ttgggcgaa 
      gcacatgca ccttgcgga tgtaaacaa tggcaagag 
      tggcagatc aaaatcgcc gaaattaaa ccggaagag 
      cttaaagct tcgtgcagc ggaagcgaa gatgccgcc 
      aactgcctc tggcttgtc accaactcg aaaccctgt 
      ccaaactgc aaaagtcca atccaaaaa aacgaaggc 
      tgtaaccac atgaagtgt tccaaatgc aaattcgac 
      ttttgttgg gtgtgtcaa gaatcgtgg aagagacac 
      agttccgcg accgggggc tatttccgc tgtaaccgc 
      ttcgaggcc gttcataag gccgacgaa aagcaagga 
      aatttaatt tccgaagcg ctagacaga aataatcaa 
      atgcaagaa atgtctcgt tttttacat ttttatacg 
      cgctttaga aaccacgaa aacagccag aagctcgaa 
      gaaccgctc ctaaccagc gttcggcaa aaaagggaa 
      gtgctcgcc agcagtctc ggattaaaa agcaccgaa 
      gatttcggg gagaaagga actaaattc atcgaagac 
      ggtgtgcgg gaattatta aaagcccgg agagtttta 
      tgcggttct tacgtttac ggttattac ctggaagac 
      gacggctat aacaaaacc atctttgaa tttatgcag 
      aacgagttg gaggaagtg accgaaaag ttgagcgaa 
      atgatagcg cgcccctac ttgcgcaca ccgaaagcc 
      gtgattgtg caaaccaca gccctggcg aggcgaaaa 
      aggcacgaa ttcgtgagg gcagtggcg caagggctg 
      atcccgccg gaaacgccg ccctctcaa agaaaacgc 
      aagcgaaaa tatctcgac actgattta acgcaaaca 
      agcgcctgg ttgcgtgac tgtgaatgg cctgaatac 
      gattcagac gaggataat gaagtaagt ttcaaacaa 
      ttttctgga ggctctcca aactccaaa gagcatcaa 
      tgcggcttt ttgtgcagc agacacgga tgtggccgg 
      tctgattac aacatggag ctgataata gctctcgag 
      atgtctaga cttcagatg attgaagat aggatgaaa 
      caagcacaa gccaatgca atcacgccc gatcccagc 
      tcgagtatt tcgaataat agcaacgaa agtgccgat 
      gatcagctc aaattggct attcagctc agtttgcag 
      gagtcgggg gcggcggaa gagacggtt cagagccaa 
      ccgtatcaa aagagcagt gcggactgg acggttgat 
      tacttcctc aagtcttta gcgaaccac aaaattgat 
      ctgaagagt ctcgatgtt aacaaactt ggcacggag 
      actgacagg gagttgttc tttcggcct tgtaacggg 
      aatgaagat ggaaggttc caaatttct gatattcac 
      gaaaaaggt tttgcgttt gaagatgtt gatgattat 
      gatgatgcg gacgttggt ctgaaacgc tcacactcg 
      actggagat ctctgtgtc cgaagaagt gggagagga 
      acaagggtt cggatgaat ggagatgaa cctcgttac 
      cacttggat tcagaccat agcagtcag catgatgat 
      aaaccggaa gatctggcc cggaaaatt ctagctttg 
      ccttccacc tccgtgcgt actaaacaa tttcttttg 
      cttcatcat acttaccca gaggaagag tcgtatcaa 
      gggcaaagc tcgatcgaa gatactacg acttcggat 
      tcgataaac gccgacatg gaagtttgt ggtattttg 
      agttcggtg accgatgaa gacattggt aaaagttac 
      aacgaggaa gaagaaata aagaagact gaaatgaca 
      aaaatagtc gaatgtaag aaacaaacc cacaatcct 
      ttcgcctct ctgaaggcg aaattgtgt tcagcacac 
      ctgcctaaa actagttac ttgacgcgg attgcagtg 
      aacaaaagc ataggactt ctcagtagt gatcataaa 
      gtaaaaaag aactgtgat atcctcaaa gatgaccga 
      tgcaagagc gaaaagcac agccccaaa agcataaaa 
      catcagaag tgttgccgc ggcggtagt ttctccaag 
      tccacggga ttcctgttg gaagaccag cggaaaagt 
      tgcaacttg cggataaaa atctgcaac agtcccgac 
      tccaagtgc cgcaaaggc cacactctg gagacggac 
      agttcgaac gagtacgaa tccgagact ggcatcccc 
      aagtcaccc acgttgttc atatcggga gtgtccatc 
      tcccgaacg ccagaacca aaatcaccc gggatgatc 
      ctaatagcg ggtggtaga caatcgagg tcgtctagt 
      ttaagtgta cctgtgcct ctaacttcg tgttcttta 
      aacatttct agtactagt cttaacaat actctacca 
      ccggaaccg ttcacgcca tctctggtc cgttcgaca 
      accccagcc aattcacgc tcgaatatc cccagtccc 
      tcgaactcg agtcccaaa aaagaagcc aatgccaaa 
      tcgaagagc gccagcgag ccggaacgc gagagggaa 
      atgttcagg tttcccaag tcttcgacg gacggtgct 
      ttgagctcg gtcttgcac attcaagaa tcgaattta 
      agttcggac gacttccac gaagcgcta ttcctgctg 
      gaacggtct cccaaaacg agagactca aaacggcga 
      aagaaatcc aagaagaat aaagagaaa gatgataaa 
      aaggaggac gttagctcc gtattgtaa ctacgtaag 
      actgtttcg ttgttattg ttgttaaaa acgtatcga 
      aatgcgttt ttgtgataa gtagccatg taagttttt 
      gtgctaaat tttaagtga ccaacatcc attgggcga 
      ttgctgcag ttaactaac ctatttaca caatatact 
      ttgatcttc aaaacctca acattttgc aaaaatatt 
      aatttttaa acaatttaa agaggctaa gcagcttaa 
      acatacaca cattgtaaa gtggtctga attattgtg 
      ctagaaagt ttttcttgc ccaaattgt atgaaacag 
      aaatttttt atttcacaa aacctattc gagttatgg 
      tgccataac atgtataaa acgaacagt catcagttt 
      ccctgtaat gtgaacaca agtttggga aaagtggct 
      gtaaattat taataaact taaaaatat tgtgtaata 
      ctaaaaata ttgtaaata ctgatgtat aaatttgta 
      ttaatagca aaacatctc attctttaa aaattctta 
      ctaaagata aaggtaatg agagatatc atgaaagta 
      cacgatttt gattacgat tgagacaac aaaattaaa 
      ctccaaacc ttttctaac taaatgtga tattttctg 
      aataaatca gttttctga ttatggttt tgcttggcc 
      tcttgtcct gccacatgt tctgtgtgt tttataatg 
      ttttaggaa aatcatatc actgaaaat ttgttaacg 
      aaattattg tcgtttggc gagactttt gtaatattt 
      tttgtagaa gatttttat aaaatttta tataagcac 
      acatttctt cgtttcttc ttttcgaaa ctttaggcc 
      taaaattta ttttcgact gaataagaa tatagtatt 
      aaaattcat agtgatatt ttcacttgt aaaggtaaa 
      taactttat actcgttat tttttaatt aatttaagt 
      gaagtaaat gtgagtatc ctcaaactt gccagattc 
      tagattaaa caatacgtt aaggcaaac actgaaagt 
      aatgagaaa tatataccc ccgttttag actgatttt 
      cttatttat gtaaaaata tttaaagga aatagtttt 
      ttaaagtgg atttatttt tgattcgta atattatta 
      accaactgt ctttgcatt tatatgtgc gcacaatat 
      acctaccta tacctactt tggttctta tataacatt 
      gccaattaa tctgaaaat attaaacat caataaatt 
      catgcacga attttttcc atctattgt caacgaaat 
      tgagatttt aattatttc ttttgattc agccgactt 
      taaagattg gacaataga tcctcaacc gaatgaaag 
      cttaattca agctgtcaa aaaaatgtt ttgttcgct 
      gtttattcg tgtttgata ttttcctaa tgaaatgag 
      acatgtaga atataaaaa tgtaatgtt gaatgttct 
      gtacctata acagtagcc gttagaaga tgaaacttg 
      tactgattg tgatttttt tagcagata ataaataaa 
      ccatttctg aaacg
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