| General information | |||
| TC009974 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 10842988..10849775 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC009974 | ||
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_04736 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC009974 RA
MGSTSSKFK KYLQHGDEF AAMQIYQSS PELRKNLDP NLSYGENHN HNTALHYAA KHGMKHLLR TFLNDLGGN PNKKNGLNE SVLHSACQL NQTKSFSAQ ERRAACVQL LLYWKGGPL ANGGRERVD LSSQDKEGN TALHWAAST GLKRCVELL LAHGAPLFI ENNDKLTPC DLAMRGSHH DIARLLESR MVFADNADL VNEDEIITE QEEVYSGLR SQDLQEAKD QLLVDTSDM LKIPLFTAE ALLRNNEWS RELLLEKWM KDPVECCQI AGVQAPSSV LKHAGSLES SISAETNDE NEIMCEICL STISNWEQP VKMSCKHSF CKTCWESYL TTKIQDGDA HHILCPAYQ CHILVPVEL IEKLVSPDM ARRYLQFDI KAFVESNKS IKWCPIAGC GRAVRLPEA EQTGNRVNN KSAPITSHA VDCGNAHFF CWECLGEAH APCGCKQWQ EWQIKIAEI KPEELKASC SGSEDAANC LWLVTNSKP CPNCKSPIQ KNEGCNHMK CSKCKFDFC WVCQESWKR HSSATGGYF RCNRFEAVH KADEKQGNL ISEALDRNN QMQEMSRFL HFYTRFRNH ENSQKLEEP LLTSVRQKR EVLASSLGL KSTEDFGEK GTKFIEDGV RELLKARRV LCGSYVYGY YLEDDGYNK TIFEFMQNE LEEVTEKLS EMIARPYLR TPKAVIVQT TALARRKRH EFVRAVAQG LIPPETPPS QRKRKRKYL DTDLTQTSA WLRDCEWPE YDSDEDNEV SFKQFSGGS PNSKEHQCG FLCSRHGCG RSDYNMELI IALEMSRLQ MIEDRMKQA QANAITPDP SSSISNNSN ESADDQLKL AIQLSLQES GAAEETVQS QPYQKSSAD WTVDYFLKS LANHKIDLK SLDVNKLGT ETDRELFFR PCNGNEDGR FQISDIHEK GFAFEDVDD YDDADVGLK RSHSTGDLC VRRSGRGTR VRMNGDEPR YHLDSDHSS QHDDKPEDL ARKILALPS TSVRTKQFL LLHHTYPEE ESYQGQSSI EDTTTSDSI NADMEVCGI LSSVTDEDI GKSYNEEEE IKKTEMTKI VECKKQTHN PFASLKAKL CSAHLPKTS YLTRIAVNK SIGLLSSDH KVKKNCDIL KDDRCKSEK HSPKSIKHQ KCCRGGSFS KSTGFLLED QRKSCNLRI KICNSPDSK CRKGHTLET DSSNEYESE TGIPKSPTL FISGVSISR TPEPKSPGM ILIAGGRQS RSSSLSVPV PLTSCSLNI SSTSLNNTL PPEPFTPSL VRSTTPANS RSNIPSPSN SSPKKEANA KSKSASEPE REREMFRFP KSSTDGALS SVLHIQESN LSSDDFHEA LFLLERSPK TRDSKRRKK SKKNKEKDD KKEDVSSVL
- >TC009974 RA
atgggaagc acttcatcc aaattcaaa aaatatctc caacacggc gatgagttt gcagcgatg caaatttac caaagttcc ccggaactc cgcaaaaac ctggaccct aatttaagt tacggcgaa aaccacaac cacaacact gctctacat tacgctgcc aaacacggt atgaagcat ctactgagg acgtttcta aacgatttg ggaggcaat ccgaacaag aaaaacggc ttaaacgaa tcggttcta cacagcgct tgccagctc aaccaaacc aaaagtttc agcgcacaa gagagaaga gctgcatgt gtccaactg ctcctctat tggaaagga gggccgcta gccaacgga ggccgagaa agggttgat ttaagctcg caagataag gagggcaat acggccctg cactgggcc gcctccacc ggcctcaag cgctgtgtc gaactctta ctagcacat ggagctccc ctttttatc gaaaacaac gataaatta actccgtgc gatttagca atgaggggg tcccatcac gacattgct agactgcta gaaagccgg atggttttt gctgataat gctgatttg gtgaacgaa gacgagata attacagaa caagaggaa gtttacagt ggacttaga agtcaagat ctacaagaa gctaaagac cagttgctt gttgatacg tccgatatg cttaaaatc ccattattt acagccgag gctcttttg cgaaataat gaatggtcc cgtgagctt cttcttgaa aagtggatg aaagatccg gttgaatgt tgccaaatc gctggtgtt caagctcct tcttccgtt ctcaaacat gcaggttcc ttagaatcc agtatttca gcagaaact aacgacgag aatgaaata atgtgtgag atttgtttg tcgacaata tcaaactgg gagcaacca gttaaaatg tcctgtaag cactccttt tgtaaaact tgctgggaa agctacctc acaaccaaa attcaggat ggcgatgcc catcacatc ctctgtcca gcttatcag tgtcacatc ttagttccc gttgaactt atcgagaag ttagtaagt cccgacatg gcgcggaga tatctccag ttcgacata aaggccttt gtcgagagc aataaaagc atcaaatgg tgtcccatc gccggttgc ggccgagcg gtccggtta ccggaagcg gaacaaacc ggaaaccgg gttaacaat aaatcagct ccgataacc tcgcacgcg gtggactgc ggaaacgcg cattttttc tgttgggag tgcttgggc gaagcacat gcaccttgc ggatgtaaa caatggcaa gagtggcag atcaaaatc gccgaaatt aaaccggaa gagcttaaa gcttcgtgc agcggaagc gaagatgcc gccaactgc ctctggctt gtcaccaac tcgaaaccc tgtccaaac tgcaaaagt ccaatccaa aaaaacgaa ggctgtaac cacatgaag tgttccaaa tgcaaattc gacttttgt tgggtgtgt caagaatcg tggaagaga cacagttcc gcgaccggg ggctatttc cgctgtaac cgcttcgag gccgttcat aaggccgac gaaaagcaa ggaaattta atttccgaa gcgctagac agaaataat caaatgcaa gaaatgtct cgtttttta catttttat acgcgcttt agaaaccac gaaaacagc cagaagctc gaagaaccg ctcctaacc agcgttcgg caaaaaagg gaagtgctc gccagcagt ctcggatta aaaagcacc gaagatttc ggggagaaa ggaactaaa ttcatcgaa gacggtgtg cgggaatta ttaaaagcc cggagagtt ttatgcggt tcttacgtt tacggttat tacctggaa gacgacggc tataacaaa accatcttt gaatttatg cagaacgag ttggaggaa gtgaccgaa aagttgagc gaaatgata gcgcgcccc tacttgcgc acaccgaaa gccgtgatt gtgcaaacc acagccctg gcgaggcga aaaaggcac gaattcgtg agggcagtg gcgcaaggg ctgatcccg ccggaaacg ccgccctct caaagaaaa cgcaagcga aaatatctc gacactgat ttaacgcaa acaagcgcc tggttgcgt gactgtgaa tggcctgaa tacgattca gacgaggat aatgaagta agtttcaaa caattttct ggaggctct ccaaactcc aaagagcat caatgcggc tttttgtgc agcagacac ggatgtggc cggtctgat tacaacatg gagctgata atagctctc gagatgtct agacttcag atgattgaa gataggatg aaacaagca caagccaat gcaatcacg cccgatccc agctcgagt atttcgaat aatagcaac gaaagtgcc gatgatcag ctcaaattg gctattcag ctcagtttg caggagtcg ggggcggcg gaagagacg gttcagagc caaccgtat caaaagagc agtgcggac tggacggtt gattacttc ctcaagtct ttagcgaac cacaaaatt gatctgaag agtctcgat gttaacaaa cttggcacg gagactgac agggagttg ttctttcgg ccttgtaac gggaatgaa gatggaagg ttccaaatt tctgatatt cacgaaaaa ggttttgcg tttgaagat gttgatgat tatgatgat gcggacgtt ggtctgaaa cgctcacac tcgactgga gatctctgt gtccgaaga agtgggaga ggaacaagg gttcggatg aatggagat gaacctcgt taccacttg gattcagac catagcagt cagcatgat gataaaccg gaagatctg gcccggaaa attctagct ttgccttcc acctccgtg cgtactaaa caatttctt ttgcttcat catacttac ccagaggaa gagtcgtat caagggcaa agctcgatc gaagatact acgacttcg gattcgata aacgccgac atggaagtt tgtggtatt ttgagttcg gtgaccgat gaagacatt ggtaaaagt tacaacgag gaagaagaa ataaagaag actgaaatg acaaaaata gtcgaatgt aagaaacaa acccacaat cctttcgcc tctctgaag gcgaaattg tgttcagca cacctgcct aaaactagt tacttgacg cggattgca gtgaacaaa agcatagga cttctcagt agtgatcat aaagtaaaa aagaactgt gatatcctc aaagatgac cgatgcaag agcgaaaag cacagcccc aaaagcata aaacatcag aagtgttgc cgcggcggt agtttctcc aagtccacg ggattcctg ttggaagac cagcggaaa agttgcaac ttgcggata aaaatctgc aacagtccc gactccaag tgccgcaaa ggccacact ctggagacg gacagttcg aacgagtac gaatccgag actggcatc cccaagtca cccacgttg ttcatatcg ggagtgtcc atctcccga acgccagaa ccaaaatca cccgggatg atcctaata gcgggtggt agacaatcg aggtcgtct agtttaagt gtacctgtg cctctaact tcgtgttct ttaaacatt tctagtact agtcttaac aatactcta ccaccggaa ccgttcacg ccatctctg gtccgttcg acaacccca gccaattca cgctcgaat atccccagt ccctcgaac tcgagtccc aaaaaagaa gccaatgcc aaatcgaag agcgccagc gagccggaa cgcgagagg gaaatgttc aggtttccc aagtcttcg acggacggt gctttgagc tcggtcttg cacattcaa gaatcgaat ttaagttcg gacgacttc cacgaagcg ctattcctg ctggaacgg tctcccaaa acgagagac tcaaaacgg cgaaagaaa tccaagaag aataaagag aaagatgat aaaaaggag gacgttagc tccgtattg taa
- >TC009974 RA
ttctgatac acactattt ggtcgctat catgaaaat gtcaattcc ttgtcacaa atatcgcaa aatagataa agaaaatgt cagatagta actcgccct gttactggc tgagaaaat gggcatttt cgacacgaa gctgcgttt cttgacaag aaaccgacc gctaacaag tacacatat cgaattttt ttatacaac cgtttgaaa ttagtttag aactccctc aacataatg ggaagcact tcatccaaa ttcaaaaaa tatctccaa cacggcgat gagtttgca gcgatgcaa atttaccaa agttccccg gaactccgc aaaaacctg gaccctaat ttaagttac ggcgaaaac cacaaccac aacactgct ctacattac gctgccaaa cacggtatg aagcatcta ctgaggacg tttctaaac gatttggga ggcaatccg aacaagaaa aacggctta aacgaatcg gttctacac agcgcttgc cagctcaac caaaccaaa agtttcagc gcacaagag agaagagct gcatgtgtc caactgctc ctctattgg aaaggaggg ccgctagcc aacggaggc cgagaaagg gttgattta agctcgcaa gataaggag ggcaatacg gccctgcac tgggccgcc tccaccggc ctcaagcgc tgtgtcgaa ctcttacta gcacatgga gctcccctt tttatcgaa aacaacgat aaattaact ccgtgcgat ttagcaatg agggggtcc catcacgac attgctaga ctgctagaa agccggatg gtttttgct gataatgct gatttggtg aacgaagac gagataatt acagaacaa gaggaagtt tacagtgga cttagaagt caagatcta caagaagct aaagaccag ttgcttgtt gatacgtcc gatatgctt aaaatccca ttatttaca gccgaggct cttttgcga aataatgaa tggtcccgt gagcttctt cttgaaaag tggatgaaa gatccggtt gaatgttgc caaatcgct ggtgttcaa gctccttct tccgttctc aaacatgca ggttcctta gaatccagt atttcagca gaaactaac gacgagaat gaaataatg tgtgagatt tgtttgtcg acaatatca aactgggag caaccagtt aaaatgtcc tgtaagcac tccttttgt aaaacttgc tgggaaagc tacctcaca accaaaatt caggatggc gatgcccat cacatcctc tgtccagct tatcagtgt cacatctta gttcccgtt gaacttatc gagaagtta gtaagtccc gacatggcg cggagatat ctccagttc gacataaag gcctttgtc gagagcaat aaaagcatc aaatggtgt cccatcgcc ggttgcggc cgagcggtc cggttaccg gaagcggaa caaaccgga aaccgggtt aacaataaa tcagctccg ataacctcg cacgcggtg gactgcgga aacgcgcat tttttctgt tgggagtgc ttgggcgaa gcacatgca ccttgcgga tgtaaacaa tggcaagag tggcagatc aaaatcgcc gaaattaaa ccggaagag cttaaagct tcgtgcagc ggaagcgaa gatgccgcc aactgcctc tggcttgtc accaactcg aaaccctgt ccaaactgc aaaagtcca atccaaaaa aacgaaggc tgtaaccac atgaagtgt tccaaatgc aaattcgac ttttgttgg gtgtgtcaa gaatcgtgg aagagacac agttccgcg accgggggc tatttccgc tgtaaccgc ttcgaggcc gttcataag gccgacgaa aagcaagga aatttaatt tccgaagcg ctagacaga aataatcaa atgcaagaa atgtctcgt tttttacat ttttatacg cgctttaga aaccacgaa aacagccag aagctcgaa gaaccgctc ctaaccagc gttcggcaa aaaagggaa gtgctcgcc agcagtctc ggattaaaa agcaccgaa gatttcggg gagaaagga actaaattc atcgaagac ggtgtgcgg gaattatta aaagcccgg agagtttta tgcggttct tacgtttac ggttattac ctggaagac gacggctat aacaaaacc atctttgaa tttatgcag aacgagttg gaggaagtg accgaaaag ttgagcgaa atgatagcg cgcccctac ttgcgcaca ccgaaagcc gtgattgtg caaaccaca gccctggcg aggcgaaaa aggcacgaa ttcgtgagg gcagtggcg caagggctg atcccgccg gaaacgccg ccctctcaa agaaaacgc aagcgaaaa tatctcgac actgattta acgcaaaca agcgcctgg ttgcgtgac tgtgaatgg cctgaatac gattcagac gaggataat gaagtaagt ttcaaacaa ttttctgga ggctctcca aactccaaa gagcatcaa tgcggcttt ttgtgcagc agacacgga tgtggccgg tctgattac aacatggag ctgataata gctctcgag atgtctaga cttcagatg attgaagat aggatgaaa caagcacaa gccaatgca atcacgccc gatcccagc tcgagtatt tcgaataat agcaacgaa agtgccgat gatcagctc aaattggct attcagctc agtttgcag gagtcgggg gcggcggaa gagacggtt cagagccaa ccgtatcaa aagagcagt gcggactgg acggttgat tacttcctc aagtcttta gcgaaccac aaaattgat ctgaagagt ctcgatgtt aacaaactt ggcacggag actgacagg gagttgttc tttcggcct tgtaacggg aatgaagat ggaaggttc caaatttct gatattcac gaaaaaggt tttgcgttt gaagatgtt gatgattat gatgatgcg gacgttggt ctgaaacgc tcacactcg actggagat ctctgtgtc cgaagaagt gggagagga acaagggtt cggatgaat ggagatgaa cctcgttac cacttggat tcagaccat agcagtcag catgatgat aaaccggaa gatctggcc cggaaaatt ctagctttg ccttccacc tccgtgcgt actaaacaa tttcttttg cttcatcat acttaccca gaggaagag tcgtatcaa gggcaaagc tcgatcgaa gatactacg acttcggat tcgataaac gccgacatg gaagtttgt ggtattttg agttcggtg accgatgaa gacattggt aaaagttac aacgaggaa gaagaaata aagaagact gaaatgaca aaaatagtc gaatgtaag aaacaaacc cacaatcct ttcgcctct ctgaaggcg aaattgtgt tcagcacac ctgcctaaa actagttac ttgacgcgg attgcagtg aacaaaagc ataggactt ctcagtagt gatcataaa gtaaaaaag aactgtgat atcctcaaa gatgaccga tgcaagagc gaaaagcac agccccaaa agcataaaa catcagaag tgttgccgc ggcggtagt ttctccaag tccacggga ttcctgttg gaagaccag cggaaaagt tgcaacttg cggataaaa atctgcaac agtcccgac tccaagtgc cgcaaaggc cacactctg gagacggac agttcgaac gagtacgaa tccgagact ggcatcccc aagtcaccc acgttgttc atatcggga gtgtccatc tcccgaacg ccagaacca aaatcaccc gggatgatc ctaatagcg ggtggtaga caatcgagg tcgtctagt ttaagtgta cctgtgcct ctaacttcg tgttcttta aacatttct agtactagt cttaacaat actctacca ccggaaccg ttcacgcca tctctggtc cgttcgaca accccagcc aattcacgc tcgaatatc cccagtccc tcgaactcg agtcccaaa aaagaagcc aatgccaaa tcgaagagc gccagcgag ccggaacgc gagagggaa atgttcagg tttcccaag tcttcgacg gacggtgct ttgagctcg gtcttgcac attcaagaa tcgaattta agttcggac gacttccac gaagcgcta ttcctgctg gaacggtct cccaaaacg agagactca aaacggcga aagaaatcc aagaagaat aaagagaaa gatgataaa aaggaggac gttagctcc gtattgtaa ctacgtaag actgtttcg ttgttattg ttgttaaaa acgtatcga aatgcgttt ttgtgataa gtagccatg taagttttt gtgctaaat tttaagtga ccaacatcc attgggcga ttgctgcag ttaactaac ctatttaca caatatact ttgatcttc aaaacctca acattttgc aaaaatatt aatttttaa acaatttaa agaggctaa gcagcttaa acatacaca cattgtaaa gtggtctga attattgtg ctagaaagt ttttcttgc ccaaattgt atgaaacag aaatttttt atttcacaa aacctattc gagttatgg tgccataac atgtataaa acgaacagt catcagttt ccctgtaat gtgaacaca agtttggga aaagtggct gtaaattat taataaact taaaaatat tgtgtaata ctaaaaata ttgtaaata ctgatgtat aaatttgta ttaatagca aaacatctc attctttaa aaattctta ctaaagata aaggtaatg agagatatc atgaaagta cacgatttt gattacgat tgagacaac aaaattaaa ctccaaacc ttttctaac taaatgtga tattttctg aataaatca gttttctga ttatggttt tgcttggcc tcttgtcct gccacatgt tctgtgtgt tttataatg ttttaggaa aatcatatc actgaaaat ttgttaacg aaattattg tcgtttggc gagactttt gtaatattt tttgtagaa gatttttat aaaatttta tataagcac acatttctt cgtttcttc ttttcgaaa ctttaggcc taaaattta ttttcgact gaataagaa tatagtatt aaaattcat agtgatatt ttcacttgt aaaggtaaa taactttat actcgttat tttttaatt aatttaagt gaagtaaat gtgagtatc ctcaaactt gccagattc tagattaaa caatacgtt aaggcaaac actgaaagt aatgagaaa tatataccc ccgttttag actgatttt cttatttat gtaaaaata tttaaagga aatagtttt ttaaagtgg atttatttt tgattcgta atattatta accaactgt ctttgcatt tatatgtgc gcacaatat acctaccta tacctactt tggttctta tataacatt gccaattaa tctgaaaat attaaacat caataaatt catgcacga attttttcc atctattgt caacgaaat tgagatttt aattatttc ttttgattc agccgactt taaagattg gacaataga tcctcaacc gaatgaaag cttaattca agctgtcaa aaaaatgtt ttgttcgct gtttattcg tgtttgata ttttcctaa tgaaatgag acatgtaga atataaaaa tgtaatgtt gaatgttct gtacctata acagtagcc gttagaaga tgaaacttg tactgattg tgatttttt tagcagata ataaataaa ccatttctg aaacg
