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Gene information
General information
TC009970 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 11149803..11224391
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009970
    iBeetle screen iB_04735

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009970 RA
      MDCDSGEGI MKSDEEMGR DKPSGGSSA NHNHYSKSK 
      GSESRRMVD ITRDKPIKV AVRVVVPVR DHPKFNFVG 
      KLLGPKGNS LKRLQEDTM CKMAVLGRG SMKDRHKEE 
      ELRASGDPK FQHLSEELH VEISAFATP AEAHARIAY 
      ALAEVRRFL VPDYNDDIR QEQMWEMQV LSSQRNNNK 
      GDEASGGSS SGAEYAGED RLSSETVEA GPESPQDEH 
      PALRGIQPG TPSSTETTA SATTANGSQ LSPTNGIGN 
      GPATGGVLG VGGRKRPLL AGGLRTSMT PTKRTVMSL 
      LARARAAQA KQLPSTFQR GTPERTATL VPLLREDYI 
      TGAVNLNLI
    CDS sequence
    • >TC009970 RA
      atggactgt gattctggt gagggaatt atgaaatca 
      gacgaggag atgggtcgt gacaagccg tctggtgga 
      agctccgcc aaccacaat cattactcc aaaagtaaa 
      gggagcgag agtagaaga atggtagac attacgaga 
      gacaaaccg ataaaagtg gcagtcagg gtagttgtg 
      cctgtacga gaccatcct aaatttaat tttgttggt 
      aaacttctg gggcccaag ggtaactcg ttaaaaaga 
      ctccaagaa gacaccatg tgcaagatg gcagttctc 
      ggcagaggg tcgatgaaa gacaggcac aaggaagaa 
      gaactgcga gcgagcggc gacccgaag ttccaacac 
      ctgagcgaa gaactgcat gtagaaata agtgctttt 
      gcgactcct gctgaagct cacgccagg atcgcgtat 
      gctttggcg gaagtgcga agatttctt gttccggac 
      tacaacgac gacatccga caggaacaa atgtgggag 
      atgcaggtg ctttcgagt caaagaaac aacaacaaa 
      ggagacgaa gcgagtggt ggctcgagt tcgggggcg 
      gagtatgca ggtgaagac agattgagc agtgaaacg 
      gtggaagcg ggaccagaa agcccccaa gatgagcat 
      ccagcattg cgtggcatt cagccagga accccttcg 
      tcgacggag acgacggcc agcgcgacc acggccaac 
      gggagccaa ctgtccccg accaacggc attggcaac 
      gggccagcc acaggtggg gtcctgggc gtcggcggg 
      cgcaagaga ccgctgctg gcgggagga ctgaggacc 
      tccatgacc ccaaccaaa cgcacggtc atgagtctc 
      ctagccaga gccagggca gcgcaggcg aagcaatta 
      cctagcacc tttcaaaga ggcacaccg gagcgtact 
      gcaacattg gttcctctc ttgagggaa gattacatt 
      acgggagct gttaacctt aatttaatt taa
    mRNA sequence
    • >TC009970 RA
      ttcgtgctc caaggcgtc gtgacgtgc ttctgcata 
      ataacggcg gaaagtcca tgaggaaac tcgccggtt 
      ttttctcaa ttagcagca ttgctttac actcccttt 
      catgtaaca cgaacatgc tgggaagtt ttcgctttt 
      tcagaacct gaggcaaac aagctcgtt ggaccatgc 
      tgtaaacta catccaata gggcaagca catacatac 
      accgtcttc tctcgagac agccgtcta attgtgaag 
      ttgacactg gttttattg agctgcaga ctgtaaaaa 
      cttgttgct agaacggtg tcatcagca aaatatcgt 
      atcatttgt catgtcgta aaaacttta caacccttc 
      gtttcaaaa agaacagca aaggtgctt gcccctgtt 
      gattagggg cgcgagtaa tttttgagc tgtcactgt 
      ggagtggtc ggggagtga acctatgga ctgtgattc 
      tggtgaggg aattatgaa atcagacga ggagatggg 
      tcgtgacaa gccgtctgg tggaagctc cgccaacca 
      caatcatta ctccaaaag taaagggag cgagagtag 
      aagaatggt agacattac gagagacaa accgataaa 
      agtggcagt cagggtagt tgtgcctgt acgagacca 
      tcctaaatt taattttgt tggtaaact tctggggcc 
      caagggtaa ctcgttaaa aagactcca agaagacac 
      catgtgcaa gatggcagt tctcggcag agggtcgat 
      gaaagacag gcacaagga agaagaact gcgagcgag 
      cggcgaccc gaagttcca acacctgag cgaagaact 
      gcatgtaga aataagtgc ttttgcgac tcctgctga 
      agctcacgc caggatcgc gtatgcttt ggcggaagt 
      gcgaagatt tcttgttcc ggactacaa cgacgacat 
      ccgacagga acaaatgtg ggagatgca ggtgctttc 
      gagtcaaag aaacaacaa caaaggaga cgaagcgag 
      tggtggctc gagttcggg ggcggagta tgcaggtga 
      agacagatt gagcagtga aacggtgga agcgggacc 
      agaaagccc ccaagatga gcatccagc attgcgtgg 
      cattcagcc aggaacccc ttcgtcgac ggagacgac 
      ggccagcgc gaccacggc caacgggag ccaactgtc 
      cccgaccaa cggcattgg caacgggcc agccacagg 
      tggggtcct gggcgtcgg cgggcgcaa gagaccgct 
      gctggcggg aggactgag gacctccat gaccccaac 
      caaacgcac ggtcatgag tctcctagc cagagccag 
      ggcagcgca ggcgaagca attacctag cacctttca 
      aagaggcac accggagcg tactgcaac attggttcc 
      tctcttgag ggaagatta cattacggg agctgttaa 
      ccttaattt aatttaata atttgagcg ggcggacgt 
      acaatttat taggtttat ttaatagtt aatgagaaa 
      aaggcgatt gaaaattga tcagaggcg agaaaatta 
      attggaatt attgtagaa aatgattta tcggttccg 
      gtcccggac taggcaaac actgacgac attattaat 
      ttattggtc cggaaattc gaggcttac tttacatac 
      gggcttttt tgcgcttat tttcacgat gggatgatt 
      ttaagcagt gacacagaa atttggtct aattaatgc 
      tcgtgtttt ttctgcaaa ttttcaatc gcttcgtca 
      gatgtataa acagtttct gtttggata ttttcggtg 
      atgttgact gttattcat tctaatgta atcaattat 
      tacgcaatg cacggacca aatatgaat atttataat 
      caaaacaca aaatagtag tgcaatttt gaagcgtta 
      agtttactt tttgtacag atttagatt ttatcacaa 
      cgaatttaa agaataaat taatataat aatgtaaaa 
      agaggattt gttcctata atgcatata tggatgccg 
      ttgtctttt atttgtatt agtttgttt acgtgtttg 
      acgttttat gggagatgt gtacataca tattagaga 
      aatcgcata caataggtt tcattcgct tttctagaa 
      catatcgac tagacttaa attgtaagt gtaagagta 
      cctacctgg aaaaacaca attagttca gctcgagac 
      agacgttaa agccgaact aatccgtta aagttagtt 
      gccaaatta gtttttaaa ttagacatt aatatttta 
      gataatgat tgtaacagt ggcttttaa tattgtatg 
      cgttgatta caatgagtg taatgaaaa attttatag 
      tcactgtca ttaaataat tgataaggt aaatgtaag 
      gaactaaac cgataatgt taactacct aactgtaat 
      ttttaatat tacatttta ctgttagca tgtttaaaa 
      tgtcacagt taaaaaaat taatgttaa acatattgt 
      gcttgcaat attcacaca taaagcact cgaaatttc 
      tttcttaat gtgtttatt aaatataaa aattataaa 
      ata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY