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Gene information
General information
TC009964 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 11281609..11283313
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009964
    iBeetle screen iB_04733

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 4 number 2nd lateral oviduct: 2 - Number of animals showing the phenotype: 2
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    egg production: number no/almost no eggs -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009964 RA
      MDITGSLTT QNIQGTELA SILKELDVE YTLRLPELS 
      IRDILQLLN VYMSVAQNQ ITEYKFYNK ALNALNDSL 
      PTLDKKELL QFIFYLGVR KKCLESQTM LRQCQKTMT 
      NEFIDSLTS EELCIICIS TFKTSTKII NKALLEKVK 
      KYIDGNLSV LHDFPLFVT LVKTLRHNR CQDDNLLNT 
      ISYTIIFNK TYPLYTFPT ICHIMALFA DYFYYDKNL 
      FKIITQQGL EQLNDCKFV SRNVYLAHQ LRAKDIKRF 
      LWVLSMFSD LKLVNRSVL KTLFIPKII ERIDGGEIN 
      RDVASLVDI TLYLWMLNC HPHELVPYV FTKENVQVL 
      RRDSKNHLR LNLLLYAIL IEDPALAKT LPIKPSMVR 
      ENDYSSVFQ LRKRPLFER LLKNLDKIK KDLNISKFE 
      LSYEIPHIN IVGITGYRS GGTKAVYIE VLDGYTQIR 
      NVEFDVPVG LMALKLRLL DQFEEGVLV VSQRETDDM 
      TDSELINFL LDEIKLIC
    CDS sequence
    • >TC009964 RA
      atggatatt acgggcagc ttaacgact cagaatatc 
      caaggaact gagctcgct tcaatactg aaagaactc 
      gatgtcgaa tatacctta agactgcca gagttatca 
      attagagat attttacag ttgctaaac gtgtacatg 
      tctgtggcc caaaatcaa atcaccgaa tacaagttt 
      tacaataaa gcattgaac gccttaaat gattcactt 
      cctacttta gataagaaa gagttgcta cagttcatt 
      ttctacttg ggtgttagg aagaaatgt ctcgagtcg 
      caaacaatg ctgcgccag tgccagaaa actatgacc 
      aacgaattt atcgatagt ctaacaagc gaggaatta 
      tgcatcatt tgcatctca acattcaaa actagcact 
      aaaatcatc aacaaagca ctactcgag aaagtgaag 
      aaatacatt gatggcaac ttatctgtg ttacacgac 
      tttccatta tttgtaact ttagttaaa actttgcgg 
      cataatcgc tgccaagac gataatttg ctcaacacg 
      atttcctac accattatt tttaataaa acatatccc 
      ttgtacacc ttcccaacg atatgccac attatggcc 
      ctctttgcc gattatttc tactacgac aaaaatttg 
      tttaaaata atcacgcag cagggtttg gagcagctc 
      aatgattgt aaatttgtc tcgagaaat gtttactta 
      gcgcaccaa ttgcgggct aaggatatt aagcgcttc 
      ctgtgggtg ctgagcatg tttagtgat ttgaaactc 
      gtaaatcgg agcgttttg aaaactctg tttattccg 
      aaaattatc gaacgaatc gatggggga gaaataaac 
      agggatgtc gcctctttg gtcgacatc acgctgtat 
      ttgtggatg ctcaactgc catccgcat gaactagtg 
      ccttatgta ttcactaaa gaaaatgtc caggtgttg 
      cgaagggat tccaaaaat catttaaga ctgaattta 
      ttattgtat gcaatttta atcgaggat cctgcgtta 
      gctaagacg ttaccgatc aagccgtca atggtacga 
      gaaaatgat tattcgagt gtttttcaa ttacgcaaa 
      agaccgttg tttgaaagg ctgttgaaa aacttggat 
      aagattaaa aaggatcta aacatcagt aaattcgaa 
      ttgtcgtat gaaattcca cacattaat attgtgggg 
      atcacggga tacaggagt ggaggaacc aaggctgtg 
      tatatcgaa gttttagat gggtacact caaataaga 
      aacgtcgaa tttgatgtt cctgtaggt ttaatggcc 
      ctgaagcta agactgttg gaccagttt gaggagggt 
      gttttagtg gtttcgcag agagaaacg gacgacatg 
      acggattcc gaattaata aatttttta ttggatgag 
      attaaattg atctgttga
    mRNA sequence
    • >TC009964 RA
      cttttatta tatttaaat attgataaa gaacaaagt 
      acaaatccg ataattcct gcaacaaaa accttcaaa 
      aacatacgt cagaatgta aatcatttg tacaagtta 
      agcacgcaa gaaatcatg gatattacg ggcagctta 
      acgactcag aatatccaa ggaactgag ctcgcttca 
      atactgaaa gaactcgat gtcgaatat accttaaga 
      ctgccagag ttatcaatt agagatatt ttacagttg 
      ctaaacgtg tacatgtct gtggcccaa aatcaaatc 
      accgaatac aagttttac aataaagca ttgaacgcc 
      ttaaatgat tcacttcct actttagat aagaaagag 
      ttgctacag ttcattttc tacttgggt gttaggaag 
      aaatgtctc gagtcgcaa acaatgctg cgccagtgc 
      cagaaaact atgaccaac gaatttatc gatagtcta 
      acaagcgag gaattatgc atcatttgc atctcaaca 
      ttcaaaact agcactaaa atcatcaac aaagcacta 
      ctcgagaaa gtgaagaaa tacattgat ggcaactta 
      tctgtgtta cacgacttt ccattattt gtaacttta 
      gttaaaact ttgcggcat aatcgctgc caagacgat 
      aatttgctc aacacgatt tcctacacc attattttt 
      aataaaaca tatcccttg tacaccttc ccaacgata 
      tgccacatt atggccctc tttgccgat tatttctac 
      tacgacaaa aatttgttt aaaataatc acgcagcag 
      ggtttggag cagctcaat gattgtaaa tttgtctcg 
      agaaatgtt tacttagcg caccaattg cgggctaag 
      gatattaag cgcttcctg tgggtgctg agcatgttt 
      agtgatttg aaactcgta aatcggagc gttttgaaa 
      actctgttt attccgaaa attatcgaa cgaatcgat 
      gggggagaa ataaacagg gatgtcgcc tctttggtc 
      gacatcacg ctgtatttg tggatgctc aactgccat 
      ccgcatgaa ctagtgcct tatgtattc actaaagaa 
      aatgtccag gtgttgcga agggattcc aaaaatcat 
      ttaagactg aatttatta ttgtatgca attttaatc 
      gaggatcct gcgttagct aagacgtta ccgatcaag 
      ccgtcaatg gtacgagaa aatgattat tcgagtgtt 
      tttcaatta cgcaaaaga ccgttgttt gaaaggctg 
      ttgaaaaac ttggataag attaaaaag gatctaaac 
      atcagtaaa ttcgaattg tcgtatgaa attccacac 
      attaatatt gtggggatc acgggatac aggagtgga 
      ggaaccaag gctgtgtat atcgaagtt ttagatggg 
      tacactcaa ataagaaac gtcgaattt gatgttcct 
      gtaggttta atggccctg aagctaaga ctgttggac 
      cagtttgag gagggtgtt ttagtggtt tcgcagaga 
      gaaacggac gacatgacg gattccgaa ttaataaat 
      tttttattg gatgagatt aaattgatc tgttgacgt 
      ttagttaac aatttatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY