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Gene information
General information
TC031205
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC009951
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 11368939..11370598
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031205
    iBeetle screen iB_04732

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    larva segment random number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva segment sometimes fused - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031205 RA
      MTYWQKLGV PTLNPAMLF GDTKKMFLG ECTFGEQFL 
      DIYNKFKAR GVKHGGVYL GIKPFYLPL EPELVKHIM 
      QNDFEHFVN HGLYMDKEN DPLSGHLFN LEDEKWKNM 
      RNKLTPTFT SGKLKMMFQ TLANCTVGL QTIMDDADT 
      NHTPLDIKD ILGRFTTDI IGSVAFGLE CNSLKDPDA 
      LFRKYGRKV FEMDIWERL KMFTQFVLS HRVLLIKDT 
      VEYREKNNV SRKDFMHLL LQLKNRGSV TDDDKVTDD 
      HGKAQEKAL TLNELSAQA FVFFIAGFE TSSTTMTFA 
      LFELSTNPH IQDKLREEI NQVLEKYDD KLTYDAMME 
      LTYMEKVIN ETLRKYPPA PVLFRRCNK DYKVPNTSI 
      IIQKGIDVA IPVLGLHTD PDYYPNPED FDPERFSED 
      NKNKRPGFT WLPFGEGPR VCIGLRFGV LQTKVGLTA 
      LLKNYRVKL SPKTKMPLK MDKTSDFCR RRNLA
    CDS sequence
    • >TC031205 RA
      atgacctat tggcaaaaa ctaggggta ccaaccctg 
      aacccagcg atgctattc ggtgacact aaaaaaatg 
      ttccttgga gaatgcact tttggtgaa caatttctc 
      gacatttac aataagttt aaagcgcgt ggtgtaaaa 
      cacggtggt gtgtatttg ggtattaaa cccttttat 
      ctaccgtta gaaccggaa ctcgtcaaa cacatcatg 
      caaaacgat ttcgagcat tttgttaat cacggactt 
      tacatggac aaggagaac gaccctctg agtggacat 
      ttatttaac ttggaggat gagaaatgg aaaaacatg 
      cgaaataaa ttaacaccc actttcact tccgggaag 
      ttgaaaatg atgttccag actttggct aattgcact 
      gtcggctta caaacaata atggatgat gctgacacg 
      aaccacact cctcttgat atcaaagac atcctaggc 
      cggttcaca actgatata atcggttcg gttgcattc 
      ggtttagag tgcaacagt ctgaaagac cctgacgcg 
      ctgtttagg aagtacggg aggaaagtt tttgaaatg 
      gatatttgg gaacggcta aaaatgttt acacagttc 
      gtgctctca catagagtt ttactaatt aaagacaca 
      gtggaatat cgagagaaa aacaatgtg tcgagaaaa 
      gattttatg catttgttg ctacagttg aagaatagg 
      ggaagtgtt acagatgat gataaagta acagacgac 
      catggaaaa gcacaggag aaagctctc actctgaac 
      gagctttcg gcccaagct ttcgtgttt tttattgcc 
      gggtttgag acttcgtcc acgactatg acttttgct 
      ctgttcgaa ctatcaaca aatcctcac attcaggat 
      aaactaagg gaagaaata aaccaagtt ttggagaag 
      tatgatgat aaattaaca tacgatgct atgatggaa 
      ctgacctac atggaaaaa gtaataaat gaaacttta 
      cgaaaatat cctcccgct cctgtccta tttagaaga 
      tgcaacaaa gactacaaa gttccgaac acttctata 
      attatccaa aaaggcatt gatgtggct ataccagtg 
      ctaggattg catacagat ccggattat tatccaaat 
      ccggaagat tttgacccg gaacgtttt tctgaggat 
      aataaaaat aaaagaccg ggtttcact tggttaccg 
      tttggtgaa ggaccaaga gtttgtatt ggtttgcgt 
      tttggagta ttacaaacc aaagtggga ctaacagct 
      ttacttaag aattatagg gtaaaatta agcccaaaa 
      accaaaatg ccattgaaa atggataaa acttctgac 
      ttctgccga aggaggaat ttggcttga
    mRNA sequence
    • >TC031205 RA
      ctatttaaa atggagaat gacctattg gcaaaaact 
      aggggtacc aaccctgaa cccagcgat gctattcgg 
      tgacactaa aaaaatgtt ccttggaga atgcacttt 
      tggtgaaca atttctcga catttacaa taagtttaa 
      agcgcgtgg tgtaaaaca cggtggtgt gtatttggg 
      tattaaacc cttttatct accgttaga accggaact 
      cgtcaaaca catcatgca aaacgattt cgagcattt 
      tgttaatca cggacttta catggacaa ggagaacga 
      ccctctgag tggacattt atttaactt ggaggatga 
      gaaatggaa aaacatgcg aaataaatt aacacccac 
      tttcacttc cgggaagtt gaaaatgat gttccagac 
      tttggctaa ttgcactgt cggcttaca aacaataat 
      ggatgatgc tgacacgaa ccacactcc tcttgatat 
      caaagacat cctaggccg gttcacaac tgatataat 
      cggttcggt tgcattcgg tttagagtg caacagtct 
      gaaagaccc tgacgcgct gtttaggaa gtacgggag 
      gaaagtttt tgaaatgga tatttggga acggctaaa 
      aatgtttac acagttcgt gctctcaca tagagtttt 
      actaattaa agacacagt ggaatatcg agagaaaaa 
      caatgtgtc gagaaaaga ttttatgca tttgttgct 
      acagttgaa gaatagggg aagtgttac agatgatga 
      taaagtaac agacgacca tggaaaagc acaggagaa 
      agctctcac tctgaacga gctttcggc ccaagcttt 
      cgtgttttt tattgccgg gtttgagac ttcgtccac 
      gactatgac ttttgctct gttcgaact atcaacaaa 
      tcctcacat tcaggataa actaaggga agaaataaa 
      ccaagtttt ggagaagta tgatgataa attaacata 
      cgatgctat gatggaact gacctacat ggaaaaagt 
      aataaatga aactttacg aaaatatcc tcccgctcc 
      tgtcctatt tagaagatg caacaaaga ctacaaagt 
      tccgaacac ttctataat tatccaaaa aggcattga 
      tgtggctat accagtgct aggattgca tacagatcc 
      ggattatta tccaaatcc ggaagattt tgacccgga 
      acgtttttc tgaggataa taaaaataa aagaccggg 
      tttcacttg gttaccgtt tggtgaagg accaagagt 
      ttgtattgg tttgcgttt tggagtatt acaaaccaa 
      agtgggact aacagcttt acttaagaa ttatagggt 
      aaaattaag cccaaaaac caaaatgcc attgaaaat 
      ggataaaac ttctgactt ctgccgaag gaggaattt 
      ggcttgata tggaaaaat tgaactaaa cagtagtaa 
      ctgaatttt atacgagta ttttgcaca atttattgt 
      gattactta ataaattat taaaatata a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY